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Summary of Contents for Sartorius Microsart Series
- Page 1 Operating Instructions Original Operating Instructions ® Microsart Manifold Vacuum Filtration System for the Microbiological Quality Control of Liquid Samples 1000061444 Manual_Microsart_Manifold_W166001-e.indb 1 28.02.19 10:03...
- Page 2 Manual_Microsart_Manifold_W166001-e.indb 2 28.02.19 10:03...
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Page 3: Table Of Contents
Contents Contents 1 About these Instructions . . . . . . . . . . . . . . . . . . . . . 6 1 .1 Scope . - Page 4 Contents ® 4 .1 .5 Microsart Manifolds for 500 ml Stainless-steel Funnel . . . . . . . . . . . . 18 4 .2 Selecting an Installation Site . . . . . . . . . . . . . 19 4 .3 Unpacking .
- Page 5 14 Sartorius Service . . . . . . . . . . . . . . . . . . .
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Page 6: About These Instructions
About these Instructions About these Instructions Scope These instructions are part of the product . These instructions apply to the product in the following versions: ® ® Product for Microsart funnel and Microsart @filter units Model ® 1-way Microsart manifold 168M1-MS ®... -
Page 7: Accompanying Documents
About these Instructions Accompanying Documents In addition to these instructions, observe the following documentation: − Operating instructions ® Microsart e .jet Transfer Pump Target Groups These instructions are addressed to the following target groups . The target groups must possess the specified knowledge . Target group Knowledge and responsibilities User... -
Page 8: Symbols Used
About these Instructions Symbols Used 1.4.1 Warnings in Operating Instructions WARNING Denotes a danger with the risk that death or severe injury may result if it is not avoided . CAUTION Denotes a hazard that may result in moderate or minor injury if it is not avoided . NOTICE Denotes a danger with the risk that property damage may result if it is not avoided . -
Page 9: Safety Precautions
You must not modify or repair the product or make any technical changes . Any retrofitting or technical changes to the product are only permitted with prior written permission from Sartorius . Personnel Qualification If persons who do not possess sufficient knowledge about safely handling the product are working on the product, those people can injure themselves or others . -
Page 10: Significance Of These Instructions
Observe the maintenance and cleaning intervals (for intervals and maintenance tasks, see Chapter “7 Cleaning and Maintenance”, page 28) . Have any malfunctions or damage repaired immediately by Sartorius Service . Action in the Event of an Emergency If there is immediate danger of personal injury or equipment damage, e .g ., due to malfunctions or dangerous situations, the product must be immediately taken out of operation . -
Page 11: Accessories, Consumables, And Spare Parts
− Loss of the sample to be tested − Contamination of the sample to be tested Only use approved accessories, consumables, and spare parts supplied by Sartorius . Only use accessories, consumables, and spare parts that are in proper working order (see Chapter “15 Accessories, Consumables, and Spare Parts”, page 43) . -
Page 12: Product Description
Product Description Product Description Product Overview ® Fig . 1: Microsart manifolds Designation Designation 6-way manifold 2-way round arch manifold 3-way manifold Serial number Base support with sintered frit Distribution number 1-way manifold “Stop” position “Start” position ® Microsart Manifold Operating Instructions Manual_Microsart_Manifold_W166001-e.indb 12 28.02.19 10:03... -
Page 13: Manifold
Product Description 3.1.1 Structure of 1-way|3-way|6-way Manifold Fig . 2: Body of 1-way manifold (example) Designation Designation Cover cap Hose with quick-lock coupling Knurled screw with flat spring Valve stopcock Ventilation hole Body of 1-way manifold Silicone foot ® Microsart Manifold Operating Instructions Manual_Microsart_Manifold_W166001-e.indb 13... -
Page 14: Manifold
Product Description 3.1.2 Structure of 2-way Round Arch Manifold Fig . 3: Body of 2-way round arch manifold Designation Designation Knurled screw with flat spring Body of 2-way round arch manifold Ventilation hole Silicone foot with adjustable Valve stopcock stainless-steel housing Connector for adaptation to the ®... -
Page 15: Base Support
. In order to filter the liquid sample through the filter, the manifold must first be connected to a vacuum source (Sartorius recommends using a Microsart e .jet vacuum pump) . -
Page 16: Installation
Installation Installation Scope of Delivery ® ® ® 4.1.1 Microsart Manifolds for Microsart Funnels and Microsart @filter Manifolds 1-way 2-way 3-way 6-way Manifold Base support for 47 mm filter Sintered frit Vent filter Cover cap to seal the output Quick coupling for adapting to the ®... -
Page 17: Microsart ® Manifolds For Biosart ® 250 Funnel
Installation ® ® 4.1.3 Microsart Manifolds for Biosart 250 Funnel Manifolds 1-way 3-way 6-way Manifold Base support for 47 mm and 50 mm filters Sintered frit Silicone seal Plug to seal the ventilation opening Cover cap to seal the output Silicone hose with quick-lock coupling, 20 cm Operating instructions ®... -
Page 18: Stainless-Steel Funnel
Installation ® 4.1.5 Microsart Manifolds for 500 ml Stainless-steel Funnel Manifolds 1-way 3-way 6-way Manifold Base support for 47 mm and 50 mm filters Sintered frit Silicone seal 500 ml stainless-steel funnel Cover (for 500 ml) Seal (for 500 ml) Plug to seal the ventilation opening Cover cap to seal the output Silicone hose with quick-lock coupling, 20 cm... -
Page 19: Selecting An Installation Site
Installation Selecting an Installation Site Make sure that the following conditions are met at the installation site: Condition Features Ambient conditions − Suitability tested (see Chapter “13 .5 Ambient Conditions at the Installation Site”, page 40 for ambient conditions) Setup surface −... -
Page 20: Unpacking
Installation Unpacking Procedure Unpack the product . If the product is stored temporarily: Observe the storage information (see Chapter “11 .1 Storage”, page 36) . Install the product at the intended installation location . Keep all parts of the original packaging, e .g . to return the product . -
Page 21: Getting Started
Getting Started Getting Started ® Integration of the Microsart Manifold in the Process Structure Requirements The product is connected with the vacuum pump (see Chapter “4 .4 Installing the Product”, page 20) . The vacuum pump is switched off . The vacuum pump is connected to the power supply at the installation site . -
Page 22: Operation
Operation Operation Preparing the Process Procedure Ensure that the base support is decontaminated before the process begins (see Chapter “7 .6 ® Autoclaving Microsart Manifold (Body) and Base Support”, page 32) . 6.1.1 Preparing the Base Support (When Using ® Microsart @filters) Procedure... -
Page 23: Starting The Process
Operation 6.1.3 Preparing the Base Support (When Using ® Stainless-Steel Funnels, the Microsart ® Funnel and Biosart 250) Procedure Remove the sterile membrane filter from the packaging using tweezers . Carefully use tweezers to place the membrane filter on the base support with the grid facing upward . Place the funnel (1) on the base support (3) and fix it to the pins provided using the bracket (2) . -
Page 24: Finishing The Process
Operation Finishing the Process Procedure After fully vacuuming the liquid sample (filtration unit or funnel no longer contains liquid), turn the valve stopcock back to the “Stop” position . Remove the membrane filter with tweezers and transfer it to a corresponding culture medium . Repositioning the Process Structure Procedure Finish the process (see Chapter “6 .3 Finishing the... -
Page 25: Removing The Cover Cap
Operation 6.4.2 Removing the Cover Cap Procedure With your thumb on the front face, push the cover cap towards the connector (1) and pull back the outer sleeve of the connector with your index and middle fingers . Then release your thumb and remove the cap from the connector . -
Page 26: Connecting Two Manifolds
Operation 6.4.5 Connecting Two Manifolds When processing a large number of samples, two manifolds can be connect to each other using a connection coupling in order to increase the filtration capacity . Procedure Hold the first manifold (1) . Push the connection coupling (2) onto the connector until the stop . -
Page 27: Removing The Base Support
Operation 6.4.7 Removing the Base Support Remove the sintered frits for cleaning purposes . The base support can also be removed for maintenance purposes . Procedure Hold the manifold at the drain pipe of the manifold (2) . Lift out the base support (1) . 6.4.8 Attaching the Base Support Procedure... -
Page 28: Cleaning And Maintenance
Cleaning and Maintenance Cleaning and Maintenance Cleaning Product Surfaces Requirements The process is complete (see Chapter “6 .3 Finishing the Process”, page 24) . Procedure Disconnect the product from the vacuum pump . NOTICE Corrosion or damage to the product due to unsuitable cleaning agents! Do not use corrosive, chloride-containing or aggressive cleaning agents . -
Page 29: Cleaning The Manifold
Cleaning and Maintenance Interval Component Action Chapter, page Weekly / Manifold Autoclave the manifold . 7 .6, 32 depending on application Annually O-ring The O-rings shift . 7 .4, 30 Only in the event Silicone foot Replace the silicone feet . 7 .7, 32 of leakage / damage... -
Page 30: Replacing The O-Rings
Cleaning and Maintenance Replacing the O-Rings Procedure Remove the base support (see Chapter “6 .4 .7 Removing the Base Support”, page 27) . Notice The O-ring is damaged by improper use . Only replace the O-ring using a suitable tool and avoid any damage . Carefully remove the O-ring from the base support groove . - Page 31 Cleaning and Maintenance Cleaning the Valve Stopcock Procedure Clean the valve stopcock using a clean cloth . Ensure that the contact surfaces are clean and free from particles . Grease the valve stopcock on the contact surface using new high-vacuum grease . Inserting the Valve Stopcock Procedure Insert the valve stopcock into the manifold until...
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Page 32: Autoclaving Microsart ® Manifold (Body)
Cleaning and Maintenance ® Autoclaving Microsart Manifold (Body) and Base Support Procedure Remove the ventilation filter or blind plug . ® Strip down the Microsart manifold system: Remove the filtration unit . Remove all hoses . Autoclave the manifold with base support . Replacing the Silicone Feet Procedure Turn the manifold upside down . -
Page 33: Malfunctions
. If the valve stopcock has stress marks, contact Sartorius Service . ® The Microsart One or more silicone Affix a new silicone foot 7 .7, 32... - Page 34 . If the possible; a high lubricated and may malfunction reoccurs: amount of force have integrated Contact Sartorius must be used for somewhat into the Service . the rotation . matching piece . The valve stopcock The Socket screw in Contact Sartorius ®...
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Page 35: Decommissioning
Maintenance”, page 28) the manifold after it has been decommissioned (see Chapter “9 Decommis- sioning”, page 35) . Sartorius recommends carrying out the internal transport of the manifold in the original packaging . The original packaging can be requested from Sartorius . -
Page 36: Storage And Shipping
Conditions at the Installation Site”, page 40) . 11.2 Returning the Product and Parts Defective products or parts can be sent back to Sartorius . Returned products must be clean, decontaminated, and packed in their original packaging . Transport damage as well as measures for subsequent cleaning and disinfection of the product or parts by Sartorius shall be charged to the sender . -
Page 37: Disposal
. WARNING Risk of injury due to contaminated products. Products contaminated with hazardous materials (NBC contamination) will not be accepted by Sartorius for repair or disposal . Operating Instructions Microsart® Manifold Manual_Microsart_Manifold_W166001-e.indb 37 28.02.19 10:03... -
Page 38: Technical Data
Technical Data Technical Data 13.1 Dimensions and Weights Unit Value Dimensions (L x W x H) ® Microsart 1-way stainless-steel manifold 176 x 120 x 98 ® Microsart 2-way round arch manifold 246 x 130 x 118 ® Microsart 3-way stainless-steel manifold 474 x 120 x 98 ®... -
Page 39: Permitted Operating Pressure
Technical Data Unit Value ® Microsart 2-way stainless-steel manifold Quick seal to connect to a ® Microsart e .jet pump 166MP-4 Permitted hoses Type: Silicone hose Outer diameter (OD) Inner diameter (ID) 13.3 Permitted Operating Pressure Unit Value Maximum operating pressure Only operate under vacuum mbar abs . -
Page 40: Ambient Conditions At The Installation Site
Technical Data 13.5 Ambient Conditions at the Installation Site Unit Value Installation site: Conventional laboratory 4000 rooms, max . height above sea level Temperature °C +5 – +40 Relative humidity At temperatures of up to 31°C Decreasing linearly at temperatures from 31°C to 40°C 13.6 Materials Stainless steel 1 .4404... -
Page 41: Approved Cleaning Agents
Technical Data Polysulfone (PSU) Coupling with hose nozzle* Polyvinylidene fluoride (PVDF) Connector*** Silicone Gasket** Seal in Biosart Adapter** Seal for funnel cover** O-ring (connector)** O-ring (base support) Hose Silicone foot Not for the 2-way round arch manifold ** Not all manifold variants *** Only for the 2-way round arch manifold 13.7 Approved Cleaning Agents Unit... -
Page 42: Sartorius Service
Sartorius Service Sartorius Service Sartorius Service is available for queries regarding the product . Please visit the Sartorius website (www .sartorius .com) for information about the service addresses, services provided, or to contact a local representative . When contacting Sartorius Service with questions about the system or in the event of malfunctions, be sure to have the product information, e .g . -
Page 43: Accessories, Consumables, And Spare Parts
Accessories, Consumables, and Spare Parts Accessories, Consumables, and Spare Parts 15.1 Accessories This table contains an excerpt of the accessories that can be ordered . For information on other products, contact Sartorius . Item Order number ® Minisart SRP25, vent filter... -
Page 44: Microsart @Filter, 100 Ml
Accessories, Consumables, and Spare Parts ® 15.2.2 Microsart @filter, 100 ml These tables contain excerpts of the consumables that can be ordered . For information on other products, contact Sartorius . Item Order number ® Microsart @filter, 100 ml, with cellulose nitrate membrane filter, 6D01--10-06--BL white with black grid, 0 . -
Page 45: Microsart @Filter, 250 Ml
Accessories, Consumables, and Spare Parts ® 15.2.3 Microsart @filter, 250 ml These tables contain excerpts of the consumables that can be ordered . For information on other products, contact Sartorius . Item Order number ® Microsart @filter, 250 ml, with cellulose nitrate membrane 16D01--25-06--BK filter, white with black grid, 0 .45 µm, in bags... -
Page 46: 100 Monitors
Accessories, Consumables, and Spare Parts ® 15.2.4 Biosart 100 Monitors Item Order number BS 100 Monitor, cellulose nitrate membrane filter, white 16401-47-06----K with black grid, 0 .45 µm, in trays BS 100 Monitor, cellulose nitrate membrane filter, white 16401-47-07----K with black grid, 0 .2 µm, in trays BS 100 Monitor, cellulose nitrate membrane filter, white 16401-47-H6----K with black grid, 0 .45 µm high flow, in trays... -
Page 47: Biosart ® 250 Funnels
Accessories, Consumables, and Spare Parts ® 15.2.5 Biosart 250 Funnels Item Order number ® Biosart 250 Funnel, sterile 16407--25----ALK 15.2.6 Membrane Filters Item Order number Cellulose nitrate, white with black grid, sterile, 11406--47----ACN 0 .45 µm, 47 mm, 100 pcs Cellulose nitrate, white with black grid, sterile, 11406--47----ACR 0 .45 µm, 47 mm, 1000 pcs... -
Page 48: Spare Parts
Accessories, Consumables, and Spare Parts 15.3 Spare Parts This table contains an excerpt of spare parts that can be ordered . For information on other products, contact Sartorius . Item Order number Blind coupling for locking the manifold 168SP-A0001 ®... - Page 49 Manual_Microsart_Manifold_W166001-e.indb 49 28.02.19 10:03...
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- Page 52 Sartorius Lab Instruments GmbH & Co. KG Otto-Brenner-Strasse 20 37079 Goettingen, Germany Phone: +49.551.308.0 www.sartorius.com The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice.
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Is the microsart ejet going to effectively filter viscous cordial samples from a 6 way stainless steel manifold with stainless steel 100mL funnels