BIOMET GPS III Manual

Process Steps
Step One: Blood Draw Step Two: Load Blood
Unscrew cap on center port No.1 and discard
Attach supplied 18 gauge needle to 60ml
syringe. Withdraw 5ml of ACD-A (citrate green packaging post.
anticoagulant). 55ml of blood is then drawn
Slowly load blood into center port.
from the patient according to facility protocol.
Mini GPS® III System: Attach supplied 18
gauge needle to 30ml syringe. Withdraw 3ml
of ACD-A. 27ml of blood is then drawn from
the patient according to facility protocol.
Spin
Close the lid by rotating the lid latch Clockwise. "Latched" indicator
will illuminate. The speed (3200 RPM) and time (15 minutes) are
preset. Press green button to start spin. Once spin is complete,
press red button to illuminate the "Unlocked" indicator. Twist latch
counter-clockwise to open lid.
Platelet Separation System
Remove protective cover on white cap and
discard. Screw white cap onto center port.
Step Four: PPP Extraction
To extract the platelet poor plasma (PPP), remove yellow cap on side
port No. 2 and connect the 30ml syringe. Slowly tilt the tube while
withdrawing. Replace yellow cap.
Push Open/Stop button on control panel. The "unlocked" indicator
will illuminate. Turn latch counter-clockwise to open lid. Place tube
into centrifuge.
Mini GPS® III System:
If using the mini kit, mini purple buckets must
be inserted into the centrifuge.
PRP Suspension and Extraction
Remove red cap on side port No. 3 and
With 10ml syringe attached, suspend the
connect 10ml syringe. Withdraw 2ml.
platelets by gently shaking the tube for
30 seconds.
Mini GPS® III System: Remove red cap on
side part No. 3 and connect 10ml syringe.
Withdraw 1ml.
Step Three: Balance
Fill GPS® III System counterbalance with 60ml of sterile saline and
place into opposite side of centrifuge.
Mini GPS® III System: Fill purple mini Counterbalance with 30ml of
sterile saline and place into opposite side of centrifuge.
Extract the remaining platelet rich plasma
(PRP) contents into the attached 10ml syringe.