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Barnstead Thermolyne Corporation LAB-LINE 460 Operation Manual

Automatic co2 incubators
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Barnstead Thermolyne Corporation LAB-LINE 460 Operation Manual

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  • Page 1 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...
  • Page 2 8/01 OPERATION MANUAL MANUAL NO. 057-588-00 REV. O LAB-LINE  AUTOMATIC CO INCUBATORS MODEL NO. WATER-JACKETED AUTOMATIC CO Models With Thermal Conductivity Sensor: 460, 460JPN, 460RH*, 460-1, 460-1CE, 462, 462-1, 462-1CE, 464, 464RH, 464-1, 464-1CE Models With Infrared Sensor: 465, 465RH*, 465-1, 467, 467-1, 469, 469-1, AIR-JACKETED AUTOMATIC CO Models With Thermal Conductivity Sensor: 490, 490RH*, 490-1, 490-1CE, 492, 492-1, 492-1CE,...
  • Page 3 TABLE OF CONTENTS Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 4 SECTION TITLE Introduction Description Specifications Features Installation Operation Maintenance Replacement Parts Warranty Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 5 BE ADVISED: IT IS MOST IMPORTANT THAT THE USER FOLLOW INSTALLATION INSTRUCTIONS EXACTLY AS WRITTEN. FAILURE TO DO SO IS LIKELY TO LEAD TO IMPROPER OPERATION, ERRONEOUS CALIBRATIONS AND POSSIBLE DAMAGE TO THE EQUIPMENT. UNDER NO CIRCUMSTANCES SHOULD THE USER ATTEMPT OPERATION WITHOUT THIS INFORMATION. THE FOLLOWING EQUIPMENT IS TO BE SUPPLIED BY THE USER: •...
  • Page 6 CERTIFICATION OF DECONTAMINATION: We cannot accept for service or credit a product that has been exposed to or contaminated with chemically or biologically toxic or infectious substances or subjected to radioactivity without first being certified as free from said contamination. Please have your Medical and/or Safety Officer sign this form certifying that proper decontamination procedures have been followed to render the product safe and free from hazards.
  • Page 7 SECTION 1 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 8 INTRODUCTION THANK YOU for selecting Lab-Line Instruments for your equipment needs. For maximum value and ease of start-up, PLEASE PROCEED AS FOLLOWS: • Inspect the carton and contents for shipping damage. Notify the carrier immediately if damage is found. • Use the Accessory Checklist when unpacking to verify that the complete unit has been received.

  • Page 9: Front View

    DESCRIPTION BASIC COMPONENTS, AN INITIAL OVERVIEW: FRONT VIEW: 1. CONTROL PANEL 6. INNER GLASS DOOR 2. REAR WALL COVER 7. INNER GLASS DOOR, KNOB 3. SIDE PANEL 8. OUTER STEEL DOOR/GASKET 4. FEET, LEVELING 9. SAMPLE PORT 5. INNER GLASS DOOR 10.

  • Page 10: Rear View

    BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T) REAR VIEW GAS INLET PORT CIRCUIT BREAKERS WATER OVERFLOW OUTLET (460/465 SERIES ONLY) TOGGLE SWITCH FOR RH DISPLAY (460RH, 465RH, 490RH, 495RH) RS232 DATA PINS CONNECTOR CORDSET POWER SWITCH OPTIONAL ALARM RELAY OUTPUT OUTLET (120 VAC UNITS ONLY) DESCRIPTION: (Con’t) INFRARED CONTROL OF CO...
  • Page 11 This sensor is impervious to relative humidity and is less susceptible to drift and re-calibration. By measuring the absorption of infrared light within a CO atmosphere, the highest level of sensitivity is provided. THERMAL-CONDUCTIVITY CONTROL OF CO This sensor measures CO within an atmosphere by the heat of conduction method and provides precise and reliable control.

  • Page 12: Specifications

    SPECIFICATIONS ELECTRICAL REQUIREMENTS, MODEL VOLTS AMPS WATTS WATER-JACKETED MODELS: 50/60 460JPN* 100 50/60 460-1* 230/240 50/60 462* 50/60 12.5 1500 462-1* 230/240 50/60 1500 464* 50/60 12.5 1500 464-1* 230/240 50/60 1500 465** 50/60 465-1** 230/240 50/60 467** 50/60 12.5 1500 467-1** 230/240 50/60...
  • Page 13 TEMPERATURE RANGE: WITH IR SENSOR (465/495 SERIES): Ambient +5ºC to 55ºC WITH TC SENSOR: Ambient +5ºC to 60ºC CONTROL: ±0.1ºC TEMPERATURE UNIFORMITY: ±0.25ºC ALGORITHM: TEMPERATURE: PID CO2: SmartGas® CARBON DIOXIDE TENSION: Range: 0% to 20% Control: ±0.1% CAPACITY PER CHAMBER: 460/490 SERIES: 6.2 cu.

  • Page 14: Control Panel

    FEATURES The control panel for all models has a series of command keys which enables the user to enter set point values for temperature, carbon dioxide, door heat and to calibrate the unit as required. LED readouts provide the user with current status information regarding the foregoing parameters.
  • Page 15 CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 COMMAND KEY: Pressing the SET key and then the CO allows the user with the Up and Down keys to enter the desired carbon dioxide level in the Incubator. •...
  • Page 16 CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 TEMP COMMAND KEY: Pressing the SET key and then the TEMP command key allows the user with the Up and Down arrow keys to establish the desired set point temperature in the Incubator. •...
  • Page 17 CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 DOOR HEAT COMMAND KEY: • When the SET key is pressed followed by the DOOR-HEAT key, the level of heat can be entered with the Up and Down keys as a percentage value being applied to the inner glass door.
  • Page 18 CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 HI-LIMIT THERMOSTAT: This is a backup temperature controller that takes charge in the event the microprocessor fails. The hi-limit thermostat will then control the temperature at slightly above the set point. HI-LIMIT STATUS LAMP: Indicates when the hi-limit is active.
  • Page 19 LED READOUT DISPLAY: ºC LOW WATER %CO2 LOW WATER INDICATING LIGHT: Low water conditions can be detrimental to chamber integrity, as well as temperature control. To warn of this condition, a solid green light will appear just below the LED display area to remind the user to take corrective action, i.e.
  • Page 20 LED READOUT DISPLAY: RH Calibrate RH Alarm Set point When the toggle switch located on the incubator’s back panel, is placed in the up position (normal operation), this LED will display actual chamber RH. Placing the same toggle switch in the down position will display the RH alarm set point and will also disable the unit’s audible alarm.
  • Page 21 TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE PROPER SEQUENCING IS IMPORTANT IN OPERATING COMMAND KEYS: To enter commands correctly, it is important to press the keys in SEQUENCE. Do not hold down one key and press another key—the action will not be accepted by the system.
  • Page 22 DOOR AJAR WARNING: In case the door is inadvertently left open beyond a 5 minute period, an audible beep is sounded and is synchronized with the flashing of the DOOR-HEAT indicator light to warn the user to correct the condition. AUDIBLE AND VISUAL ALARMS: The following table summarizes the audible and visual alarms that are initiated upon the occurrence of various events.

  • Page 23: Shipping Carton

    rear panel for user access. Enclosed with the incubator is a mating connector that can be customer wired and used for interfacing with a Central Alarm. NOTE: The connector is polarized. The rear panel outputs are as shown here: “CLOSES” on Fault (Normally Open COMMON WHILE IN OPERATION) Terminal...

  • Page 24: Electrical Requirements

    Open the carton carefully making certain that all parts are accounted for before packaging materials are discarded. After unpacking, if damage is found, promptly report it to the carrier and request a damage inspection promptly. IMPORTANT: Failure to request an inspection of damage within a few days after receipt of shipment absolves the carrier from any liability for damage.
  • Page 25 REAR COVER PANEL SHELVING/PLENUM INSTALLATION-SELF-SUPPORTING SYSTEM ASSEMBLED TOP PLENUM SHELF SUPPORT SHELF SUPPORT SIDE PANEL SIDE PANEL REAR COVER PANEL INSTALLATION: (Con’t) SHELVING AND PLENUM INSTALLATION-4 STEPS: NOTE: NO TOOLS OR FASTENERS ARE REQUIRED. THE COMPONENTS FIT TOGETHER BY HANGING FROM STANDOFFS. TOP PLENUM, HEAD-ON VIEW: Artisan Technology Group - Quality Instrumentation ...
  • Page 26 KEYWAY STUDS (ONTO WHICH THE CHAMBER’S REAR COVER PANEL KEYHOLE SLOTS WILL FIT) TOP PLENUM, DOWN VIEW: REAR KEYHOLE SLOTS (ONE IN EACH CORNER) FAN VENTS SPACERS STUDS ASSURE THAT CIRCULATION FAN BLADES DO NOT STRIKE THE PLENUM) FRONT Open the outer and inner front chamber doors. Hold the top plenum with the spacer studs up and the keyway studs toward the rear.
  • Page 27 With the top plenum properly secured to the chamber ceiling, a space on each side of the plenum is created into which the top of each of the shelf support side panels will fit. Tilt a support side panel at an angle and move it into the chamber. Carefully slide the panel over the studs protruding from one side of the chamber wall and lock it into place.
  • Page 28 STOPPER PUSHED FLUSH AGAINST INSIDE REAR OF CHAMBER WALL, SIDE VIEW OUTSIDE CHAMBER REAR INSIDE CHAMBER FRONT TOP PLENUM, UP VIEW: FRONT BASE HEAD LOCKING GROMMET FOUR KEYLOCK HOLES REAR Press a locking grommet, two supplied, into the two front keyhole slots adjacent to the stud from which the top plenum is secured.

  • Page 29: Installation Procedure

    INSTALLATION: (Con’t) INSTALLATION OF GAS FILTER INTERFACE ASSEMBLY KIT #018-514-00: KIT #018-514-00 INCLUDES: • 4, Hose Clamps (#250-331-00) • 1, In-Line Filter (#525-034-00) • 1, 10 ft. (4.57m) length of 1/4” (6.35mm) ID PVC tubing (#720-418-00) INSTALLATION PROCEDURE: See next page for illustration. •...
  • Page 30 INSTALLATION: (Con’t) INSTALLATION OF GAS FILTER INTERFACE ASSEMBLY KIT #018-514-00: (Con’t) BACK OF UNIT REAR PANEL VIEW, EXTERIOR: IN-LINE FILTER BACK OF UNIT #525-034-00 HOSE CLAMP HOSE CLAMP #250-331-00 #250-331-00 1/4” ID TUBING #720-418-00 HOSE CLAMP 1/4” HOSE BARB #250-331-00 CONNECTIONS (CO2 FITTING) SET PRESSURE AT 10 PSI.
  • Page 31 INSTALLATION: (Con’t) STANDARD AND SIPHON TYPE CO GAS CYLINDERS STANDARD TYPE (CORRECT) SIPHON TYPE (INCORRECT) 1. Compressed Gas Association 1. Warning tag indicating that #320 connector. cylinder is siphoning type. 2. Gas headspace. 2.* Aluminum ring. 3. Liquid CO filled to 68% 3.* Gold band.
  • Page 32 INSTALLATION: (Con’t) 460 SERIES, WATER-JACKETED MODELS, FILLING WITH WATER: Capacity of the water jacket is 10.7 gallons/40.5 liters of water. Fill the jacket only with distilled water. For optimum Incubator life, use a distilled water with an electrical resistivity of 500,000 ohms to 1 MEG ohm maximum, as measured between opposite faces of a centimeter cube of an aqueous solution per ASTM D 1125-82.
  • Page 33 INSTALLATION: (Con’t) INITIAL FILLING OF WATER JACKET, 460 SERIES: 1. Turn unit on. The low-water LED will display a low-water message. 2. To fill the water jacket, open the unit’s front door and locate water inlet, top left under front control panel. FRONT/LEFT OF UNIT: LED READOUT DISPLAY WATER INLET (FEMALE)
  • Page 34 INSTALLATION: (Con’t) 460 SERIES: NOTE: PUSH THE QUICK-CONNECT ON THE WATER INLET UNTIL IT CLICKS BEFORE INSERTING TUBING TO INSURE A SECURE CONNECTION. THE SMALL QUICK-CONNECT RESET BUTTON ON THE LEFT SIDE OF THE WATER INLET CAN BE PUSHED IF THE WATER INLET WAS INADVERTENTLY SET.
  • Page 35 INSTALLATION: (Con’t) DRAINING OF WATER JACKET, 460 SERIES: With the use of distilled water, periodic draining and refilling of the water jacket is not necessary. In the event the incubator is moved however, the water jacket should be drained. The drain is located at the left front bottom of the cabinet and is opened when the 1/4”...

  • Page 36: Operation

    SECTION 6 OPERATION DANGER: DO NOT USE IN THE PRESENCE OF FLAMMABLE OR COMBUSTIBLE MATERIALS OR EXPLOSIVE GASES. DO NOT USE IN THE PRESENCE OF PRESSURIZED OR SEALED CONTAINERS—FIRE OR EXPLOSION MAY RESULT, CAUSING DEATH OR SEVERE INJURY. WARNING: DO NOT HEAT ANY SUBSTANCE ABOVE A TEMPERATURE WHICH WILL CAUSE IT TO EMIT TOXIC FUMES—DEATH OR SEVERE INJURY MAY RESULT.

  • Page 37: Password Feature

    OPERATION: (Con’t) PASSWORD FEATURE: To prevent unauthorized entry to values which have been set, a password feature is available with your unit. SET, CAL, CO and TEMP keys have the numbers 1, 2, 3 and 4 below that are used to enter or disable a password. Select a 3-digit password from 001 through 999 FYI: 000 is not a valid password).
  • Page 38 OPERATION: (Con’t) SETTING OPERATING TEMPERATURE AND HI-LIMIT SETPOINT: (Con’t) • After this time has elapsed, rotate the HI-LIMIT thermostat counterclockwise while watching the red lamp. When the lamp is lit, you have adjusted the HI-LIMIT set point to be equal to the operating set point. NOTE: DO NOT LEAVE HI-LIMIT AT THIS SETTING •...

  • Page 39: Temperature Calibration

    OPERATION: (Con’t) DECONTAM™ PROCEDURE: AIR JACKETED MODELS WITH THERMAL CONDUCTIVITY SENSOR ONLY: The decontam cycle is initiated by sequentially pressing SET, TEMP and holding the UP arrow key until the temperature reaches 100ºC. The unit will hold this temperature for a period of 12 hours and the time elapsed during the cycle is displayed on the LED readout;...
  • Page 40 OPERATION: (Con’t) HINTS ON USING THE FYRITE*: The following information is intended to supplement that found in the Fyrite manual and is based on experience working with users of our Incubators. One of the most important aspects of using the Fyrite procedure and one that is frequently overlooked is the condition of the fluid.
  • Page 41 OPERATION: (Con’t) HINTS ON USING THE FYRITE: (Con’t) Inspect the Fyrite filter in the clear plastic casing between the hoses for any contamination or growth. Change it if necessary. CAUTION: MAKE SURE THE FILTER IS MOIST BEFORE YOU TAKE YOUR READING.
  • Page 42 OPERATION: (Con’t) TAKING A FYRITE READING: (Con’t) Depress the plunger and hold it down. It is very important not to allow the plunger to spring up—if this occurs. you will be repeating an already existing reading. It will not matter how many times you pump the squeeze bulb, as long as you hold the plunger down you will introduce only one sample to the Fyrite.
  • Page 43 OPERATION: (Con’t) RS232 STATISTICS FOR 465/495 SERIES INCUBATORS: (Con’t) • The output format used is as follows: PRINTER (CTS USE): DOUBLE LINE: ⇒ Temp = XXX.X C(LF)(CR) <<Line Feed/Carriage Return ⇒ CO2 = XX.X% (LF)(CR)(LF)(CR) • Capture Program (PC): Single Line: ⇒...

  • Page 44: Maintenance

    SECTION 7 MAINTENANCE BE ADVISED: NOTE: MAKE NO ATTEMPT TO SERVICE OR REPAIR A LAB-LINE PRODUCT UNDER WARRANTY BEFORE CONSULTING YOUR LAB-LINE DEALER. AFTER THE WARRANTY PERIOD, SUCH CONSULTATION IS STILL ADVISED, ESPECIALLY WHEN THE REPAIR MAY BE TECHNICALLY SOPHISTICATED OR DIFFICULT. IF ASSISTANCE IS NEEDED BEYOND WHAT THE DISTRIBUTOR CAN PROVIDE, PLEASE CALL THE LAB-LINE CUSTOMER RELATIONS DEPARTMENT AT (563) 556-2241 OR (800) 522-5463.
  • Page 45 MAINTENANCE: (Con’t) THE ALLOY CALLED STAINLESS: Stainless steel is an alloy of steel with chromium and nickel that increase the metal’s resistance to rust and corrosion. Yet, if not properly cared , stainless steel can rust and corrode. Exposure to air provides the passivation, or oxide layer coating, for clean stainless by producing a thin, durable chromium-oxide film that forms rapidly on the alloy surface to give stainless its characteristic “stainless”...
  • Page 46 MAINTENANCE: (Con’t) BE ADVISED: NEVER USE THE FOLLOWING ON STAINLESS STEEL: Aqua regia Ferric chloride Iodine Sodium acid Sodium azide Chemical spills, especially those agents listed here, should be removed as soon as possible and the stainless steel surface cleaned with mild soapy water followed by a clean water rinse.
  • Page 47 MAINTENANCE: (Con’t) STAINLESS CLEANING METHODS: (Con’t) • Citric acid based cleaners. • Bathroom tub and tile cleaners. • A mixture of 20% nitric acid and 1.5% hydrofluoric acid (or hyrochloric acid). Swab solution on surface allowing it to remain until rust is loosened. Immediately follow with a clean water rise.

  • Page 48: Replacement Parts

    SECTION 8 REPLACEMENT PARTS 120 VAC INCUBATORS (WITH PLUG): DESCRIPTION PART NUMBER Axial Fan: 160-136-00 Blower Motor: 370-278-00 Circuit Breaker, 5 Amp: 330-118-00 Circuit Breaker, 10 Amp: 330-119-00 Fuse, 1 Amp: 330-369-00 Cordset: 470-311-00 Foot, Leveling: 790-401-00 Gasket, Outer Door: 530-246-00 Gasket, Glass Door: 530-258-00...

  • Page 49: Warranty

    240 VAC INCUBATORS (WITHOUT PLUG): DESCRIPTION PART NUMBER Axial Fan: 160-136-00 Blower Motor: 370-279-00 Circuit Breaker, 10 Amp: 330-119-00 Fuse, 1 Amp: 330-369-00 Cordset: 470-311-00 Foot, Leveling: 790-401-00 Gasket, Outer Door: 530-246-00 Gasket, Glass Door: 530-258-00 Heater, Back & Bottom: 340-350-01 Printed Circuit Board Assembly: 460:...
  • Page 50 Page 1 of 2 LAB-LINE INSTRUMENTS, INC. (“Lab-Line”) warrants that the product manufactured by Lab-Line shall be free of defects in materials and workmanship for a period of time defined on the following page from the first to occur of (i) the date the product is sold by Lab-Line or (ii) the date the product is purchased by the original retail customer (the “Commencement Date”).
  • Page 51 12 MONTH PARTS WARRANTY: • All Environmental Chambers • Low Temperature B. O. D. Incubators • Animal Study Chamber • Controlled Environment Centers • Biological Work Station • Refrigerators, Freezers • Chromatography Refrigerators (5 year parts warranty on compressor only) •...
  • Page 52 FIRST IN INSTRUMENTS SERVICING SCIENCE, INDUSTRY, RESEARCH AND EDUCATION SINCE 1908. Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 53 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

  • Page 54: Accessory Checklist

    ACCESSORY CHECKLIST The following loose parts and accessories are packed with this unit. Before discarding any packing materials, please be sure that nothing has been overlooked. MODEL NO: 460, 460JPN, 460RH, 460-1, 460-1CE, 462, 462-1, 462-1CE, 464, 464-1, 464-1CE, 465, 465RH, 465-1, 467, 467-1, 469, 469-1, 490, 490RH, 490-1, 490-1CE, 492, 492-1, 492-1CE, 494, 494-1, 494-1CE, 495, 495JPN, 495RH, 495-1, 497, 497-1, 499, 499-1, 391, 391-1, 391-2, 391-3...
  • Page 55 Artisan Technology Group is your source for quality new and certified-used/pre-owned equipment SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT • FAST SHIPPING AND DELIVERY Experienced engineers and technicians on staff Sell your excess, underutilized, and idle used equipment at our full-service, in-house repair center We also offer credit for buy-backs and trade-ins •...

This manual is also suitable for:

Lab-line 460jpnLab-line 460rhLab-line 462Lab-line 460-1ceLab-line 462-1ceLab-line 462-1 ... Show all

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