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Summary of Contents for SCIEX C100HT
- Page 1 C100 Biologics Analyzer System Installation Guide RUO-IDV-09-3829-A June 2018...
- Page 2 SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX s sole and exclusive representations, warranties, and obligations.
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Page 3: Table Of Contents
Contents 1 Introduction..............................4 Hardware Overview................................5 Cartridge....................................7 Decommissioning and Disposal............................8 Chemical Precautions.................................8 2 Installation Instructions.........................10 Unpack and Place the System............................10 Unpack the System..............................10 Unpack the Controller and Monitor...........................10 Remove the Transport Lock..............................11 Connect the Cables................................12 Configure the Controller and the Software........................13 Set the Instrument ID...............................14 Install the Cartridge in the System...........................15 Verify the Alignment of the Transport System ........................16... -
Page 4: Introduction
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts not recommended by SCIEX or use of parts for any purpose other than their intended purpose can put the user at risk of harm or negatively impact system performance. -
Page 5: Hardware Overview
Introduction Hardware Overview Figure 1-1 Front and Side Panel Item Description Service door Cartridge door Sample door LED power indicator; green when the system is on, blue when it is off. Access cover Right side cover Installation Guide C100 Biologics Analyzer System RUO-IDV-09-3829-A 5 / 31... - Page 6 Introduction Figure 1-2 Back Panel Item Description Name plate FCC Compliance label Waste Electrical and Electronic Equipment label No User-serviceable Parts label For Research Use only label RoHS label RS-232 connector Voltage switch Power switch, mains supply connection, and fuse holder High Voltage label C100 Biologics Analyzer System...
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Page 7: Cartridge
Introduction Cartridge Note: Always allow the cartridge to stand in the cartridge stand at room temperature for at least 20 minutes before use. Figure 1-3 Cartridge in Cartridge Stand Item Description Gel reservoir Cartridge purge port Cartridge data key Capillary windows Cartridge stand Installation Guide C100... -
Page 8: Decommissioning And Disposal
• Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX Safety Data Sheets can be found at sciex.com/tech-regulatory. - Page 9 Introduction • Collect all spent liquids and dispose of them as hazardous waste. • Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or radioactive materials. Installation Guide C100 Biologics Analyzer System RUO-IDV-09-3829-A 9 / 31...
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Page 10: Installation Instructions
Installation Instructions Unpack and Place the System Refer to the “Checklists” in the Site Planning Guide for more information. Unpack the System WARNING! Lifting Hazard. Make sure that at least three people or a lifting device are available to lift the CE system. Follow established safe lifting procedures. Refer to the Site Planning Guide for the weights of system components. -
Page 11: Remove The Transport Lock
Installation Instructions Remove the Transport Lock 1. Open the sample door. 2. Turn the black knob counterclockwise to remove the transport lock. 3. Instruct the user to store the transport lock in a safe location for later use when moving the system. Installation Guide C100 Biologics Analyzer System... -
Page 12: Connect The Cables
Installation Instructions Connect the Cables Required Materials • In the instrument ship kit box: • System power cable • RS-232 cable • In the monitor carton: • mDP cable • Power cable • In the computer carton: • Mouse • Power cable •... -
Page 13: Configure The Controller And The Software
Click File > Exit. 7. Verify the printer for the C100HT instrument. a. In the 32 Karat Software Enterprise window, right-click the C100HT icon and then click Open offline. b. Type C100HTSW in the User name field and C100HTPrivate in the Password field, click C100HT in the Project list, and then click Login. -
Page 14: Set The Instrument Id
Installation Instructions CAUTION: Potential Wrong Result. Do not click (Maximize) to maximize any of the windows in the following step. If any of the windows are maximized, then the title bar buttons for windows in the DataReviewer software might not be visible. a. -
Page 15: Install The Cartridge In The System
Installation Instructions 3. Click (Service) and then click the Maintenance tab. 4. Click Instrument ID. 5. Type the serial number in the Instrument ID field and then click Apply. Install the Cartridge in the System CAUTION: Potential System Damage. Handle the cartridge with care. The tips of the capillaries are made of glass and are fragile. -
Page 16: Verify The Alignment Of The Transport System
Installation Instructions The Cartridge Status panel in the C100 software updates to display the cartridge type, ID, remaining runs, remaining normalization runs, status, and other information. Verify the Alignment of the Transport System Required Materials • 96-well plate • Empty buffer plate WARNING! Personal Injury Hazard. - Page 17 Installation Instructions 3. Click the Maintenance tab and then click Start. 4. Click Init to initialize and then put the 96-well plate and the buffer plate in the system. 5. Verify the M position. a. Click M and then verify that the transport system moves to the M row of the buffer plate. b.
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Page 18: Configure The Photomultiplier Tube (Pmt) Voltage
Installation Instructions 7. Finish the alignment. • If no adjustments are required, then the transport system is aligned. Click Cancel and then click Yes in the message to confirm. • If changes are required, go to step 8. 8. Type the new value in the SET X(D) field and then click SET X(D). Do the same thing for the SET X(M) and SET Z fields, and then click Save Positions. -
Page 19: Set The Photomultiplier Voltage
Installation Instructions 7. Click Send to instrument. The injection begins. 8. When the sample injection is complete, test the detector. Refer to Test the Detector. The raw counts should be between 14 000 and 15 000. • If at least one of the capillaries has a raw count that is approximately 15 000, then no changes are required. •... -
Page 20: Test The Detector
Installation Instructions b. Type RPMT and then click Send to instrument. The voltage appears below the command. 5. Change the voltage. Type WRPMTV <XXX> where <XXX> is the new voltage and then click Send to instrument. Type a value for the voltage that is based on the raw counts reported in the detector test. Refer to Test the Detector. -
Page 21: Review The Default Users
Installation Instructions At the end of the test, the status is shown and the results are saved to the SystemTest folder (by default C:\ProgramData\SCIEX\C100HT\Data\SystemTest). Review the Default Users The users in the following table are present by default. Refer to Table 2-1. -
Page 22: Troubleshooting
Troubleshooting Troubleshooting Tables Table 3-1 Initialization Symptom Possible Cause Corrective Action The system does not The power cable is not Connect the power cable. turn on. connected. The fuse is blown. Replace the fuse. Refer to "Replace the Fuse" in the Operator Guide. - Page 23 (Engage). If the problem persists, turn the system off and on. The cartridge data key was Insert the data key. If the problem persists, contact not detected. sciex.com/request-support. The cartridge door is open. Close the door. If the problem persists, contact sciex.com/request-support. Cannot start a run.
- Page 24 LEDs are lit. Refer to "Test the Detector" in the Operator Guide. If the test fails, then contact sciex.com/request-support. Blocked purge filter: Perform a filter check. Refer to "Test the Filter" in the Operator Guide. If the filter is blocked, then replace it.
- Page 25 • If the test is successful, then the C100 Biologics Analyzer system works correctly and there is an issue with the sample preparation. Contact sciex.com/ request-support. • If the test fails, then there is a problem with the hardware. There are no peaks.
- Page 26 Troubleshooting Table 3-2 Operation (continued) Symptom Possible Cause Corrective Action The enzyme activity was low. Inspect the peak intensity for the IST peak. If it is above 4 RFU, then digest the sample again, adding more PNGase F enzyme or using PNGase F from a different lot.
- Page 27 The Sciex PDF Writer software Make sure that the Sciex PDF Writer software is is not selected as the default selected as the printer for the C100HT instrument in printer in the 32 Karat the 32 Karat software. Refer to Configure the Controller software.
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Page 28: Run A Test Sample
Troubleshooting Run a Test Sample This test can be used to determine if there is an issue with the system hardware. Required Materials • 12-well tube strip • 20 µL pipette • Calibration solution • C100 Glycan Labeling and Analysis buffer tray 1. -
Page 29: Export Log Files For Troubleshooting
Troubleshooting Figure 3-1 Test Sample Gel Image Figure 3-2 Test Sample Electropherogram Export Log Files for Troubleshooting This option is only available to users with the Basic User or Advanced User role. Note: This option is also available to users with the Service User role. Installation Guide C100 Biologics Analyzer System... - Page 30 Troubleshooting 1. In the C100 software, click to go to Service mode. 2. In Service mode, click System Check or Maintenance. 3. At the bottom of the pane, click Troubleshooting folder. 4. In the Troubleshooting Setup dialog, specify the settings for the export. a.
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Page 31: Service Kit Bill Of Materials
Service Kit Bill of Materials Note: In addition to the Service Kit (PN C21029), a C100 Glycan Labeling and Analysis cartridge (PN C15918) is required. Part Description Quantity Number C13796 12-well tube strip (5-pack) 609801 96-well sample plate C13795 C100 buffer tray (3-pack) C13794 Calibration solution...
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