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DANA DI-100F User Manual

Inversed fluoresence microscope
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Inversed Fluoresence Microscope
DI-100F
USER MANUAL
This manual is written for Metallurgical Microscope of DI-100F ..For safety and for
exerting the best performance, making you familiar with the instrument entirely, it is
strongly recommended that you read this manual carefully before using the microscope.

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Summary of Contents for DANA DI-100F

  • Page 1 DI-100F USER MANUAL This manual is written for Metallurgical Microscope of DI-100F ..For safety and for exerting the best performance, making you familiar with the instrument entirely, it is strongly recommended that you read this manual carefully before using the microscope.

  • Page 2: Table Of Contents

    Content DI-100F …………………………………………………………………… Contents ………………………………………………………………… User Notices ……………………………………………………… 1．Components Name ………………………………………………………………… 2．Assembly 2.1 Installment figure……………………………………………………… 2.2 Installment steps…………………………………………………… …………………………………………… 3.Adjustment and opertation 3.1 Lamp adjustment for fluorescence observation ………………… 3.2 Lamp adjustment for biological observation……………………… …………………………………… 4Microscope photography and video 4.1Microscope Photography………………………………………………...

  • Page 3: User Notices

    Notices DI-100F Safety Note 1. The epi-fluorescent attachment is a precise instrument. Open the box carefully, and avoid dropping the accessories to ground and causing damage to them. 2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and virations.
  • Page 4 DI-100F Maintenance and Storage Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). Since solvents such as ether and alcohol are highly flammable, they must be handled carefully.

  • Page 5: 1．Components Name

    Phase contrast slider Lamp eyepiece Illumination bracket Condenser Protection barrier Stage Stage inserted plate Objectives nosepiece 。 4× 。 10× 。 20× 。 40× Body NFP-1 Fluorescence power supplier reflected fluorescence units B excitation G excitation DI-100F inverted fluorescence microscope...

  • Page 6: 2．Assembly

    2Installation DI-100 2.1 Installing diagram The following figure shows the installation sequence of the components. The number in the figure shows the installation steps.  Before installing, be sure every components is clean, do not score any parts or glass surface. Keep well with the supplied hexagon wrench.

  • Page 7: Installment Steps

    2.2 Installment steps DI-100F 2.2.1 Installing trinocular head(fig 1) ① Loosen the setscrew ② and insert the trinocular Viewing Head ① into the body correctly , screw down with bolt③. ③ ② 2.2.2 Installing eyepiece(fig 2) 图 1 Insert the eyepiece④ into tube until they are against.
  • Page 8 DI-100F 2.2.4 Installing lamp house(fig4、fig5) Insert the plug A into hole A of power cord. then insert theplug B into the B hole of the condenser till there are against. (Fig 6)。 Replace lamp 1. Turn the swith to off position when using or need replacement.pull out the lamp house and...
  • Page 9 DI-100F 2.2.6 Installing the objective (fig8、fig9) 1. Turning the coarse fusing knob① like the figure shows till the nosepiece get to its lowest position. ★For ensuring the safety of the instruction on transportation, the nosepiece is located in the lowest position and the tension adjustment collar②...
  • Page 10 DI-100F ● Be sure to use a GCQ-100 mercury burner. ② ③ ● Be sure to mount positive pole(the wide head) before the other , or the damage to the burner may occur. ● Never subject the burner to excessive force when mounting the Mercury Burner.
  • Page 11 DI-100F 2.2.10 Installing the stage lengthen splint and ① the mechanical ruler (fig 14、15) ◎ Stage lengthen splint can be installed in either side of the stage to enlarge the work surface. But you can’t install the mechanical ruler together.

  • Page 12: Adjustment And Opertation

    3. Adjustment and operation DI-100F 3.1.1 Connecting power Set the main switch of the power supply unit to“I” （ON） . It will stabilize in 5 to 10 minutes after ignition. ● Some mercury burners may not ignite the first time the power is turned on due to variance in production, and the safety mechanism in the starter in such a case.
  • Page 13 DI-100F 3.1.4 Adjusting the interpupillar distance (fig3、4) When observing with two eyes, hold on the left and right prism holder, turn around the axis, adjusting the interpupillar distance until the left and right fields of view coincide completely. ★ The reticle on the interpupillar distance indicator③, pointed by the spot “.”...
  • Page 14 DI-100F 3.1.7 Centering mercury burner (Fig.4-6) ◎Before proceeding to center the burner, wait for the arc image to stabilize to protect against glare during arc image centering, it should be viewed across the excitation light protective shield. 1． Switch the light shutter ① to“●” position to shut off the light path.
  • Page 15 Note： ● When the hour counter indicates ”100.0”,set the main switch to “o”( OFF) for safety, wait for more than 10 minutes, then replace the lamp burner after making sure that the lamp housing has cooled down.. A mercury burner seals high-pressure gas inside. If the burner is used beyond its service life, stress may accumulate inside the burner, and in the worst (but very rare) case, the burner could explode.。...
  • Page 16 DI-100F Centering the mirror reflected image (Fig.6) ★ The mirror reflected image has been centered before leaving the factory. Do not adjust the knob⑦ please if not necessary. Only when the burner has been centered precisely, can the knob⑦ be adjusted.
  • Page 17 DI-100F 3.1.9 Using color filters（fig11） ◎ Selecting the appropriate color filters according your need, it became more effective to observe or photography the specimen. Especially, we suggest using the LBD color filter, which can compensate more neutral colors. ◎ You could pile up a group of color filters to the filter holder, if you ensure they are level and the whole thickness is less than 11mm.

  • Page 18: Lamp Adjustment For Biological Observation

    DI-100F 3.2 Reflected observing illumination adjustment 3.2.1Turn power,adjusting brightness(fig 13) ② Connect the power, turn on the main switch①(shown on the fig.16)which on the bottom side of the base to “－”(on) Turning the brightness adjustment knob ① clockwise, the voltage raise, and the brightness strengthen;...
  • Page 19 4. Using the CT to look the light ring’s image① and the phase contrast ring’s image②, if the light ring’s image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring②. 5.

  • Page 20: 4Microscope Photography And Video

    4.Microscope photography and video DI-100F 4.1 Microscope video 4.1.1 selecting the light path（fig.1） ★ just used in the trinocular observation Pulling out the light path selector lever, until you heard the “clicked”. ★ In the dark specimen observation, you can make the focus by both eyes at first, then change the light path.
  • Page 21 DI-100F 4.2 Microscope photography 4.2.1 selecting the light path camera ★ just used trinocular observation The operation diagram is shown iand the details reference is in 4-1-1。 4-2-2 Installing the photography set （fig.3） 1. Loosen the locking bolt① on the trinocular viewing tube, and take out the dust cap②.

  • Page 22: 5．Technical Specifications

    5. Outfit DI-100F 5.1 Specification Optical system Infinite optical system DI-100F Excitation units excitation Dichroic Barrier mirror filter ● Blue Excitation BP460～490 DM500 BA510 Reflected light ● Green Excitation BP480～550 DM570 BA590 source Ultraviolet BP330～385 DM400 BA650 ● Excitation Violet Excitation BP400～410...
  • Page 23 stroke:37.7mm per rotation. Fine stroke :0.2mm per rotation  Use indoor  Altitude: Maximum 2000 m  Operation Temperature: 5℃~40℃（41°F~109°F）  condition Maximum Relative Humidity: 80% at 31℃（88°F）, then Fall Linear.  70% at 34℃（93°F）, 60% at 37℃（104°F）,50% at 40℃（104°F）. ...

  • Page 24: Objective Specification

    DI-100F 5.2 Objective Specifications COVER NUMERICAL WORKING CONJUGATE FOCUS SLIP TYPE MAGNIFICATION APERTURE DISTANCE DISTANCE DISTANCE THICKNESS （N.A） （mm） （mm） （mm） Infinite ∞ ─ 0.13 16.3 Long Working Distance ∞ Plan 1.2mm Achromatic Objective Infinite ∞ 0.17 0.25 Long Working Distance ∞...

  • Page 25: 6．Troubleshooting

    6. Troubleshooting DI-100F Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s performance. If the problem is happened, please take proper measures according to the follow table. If you can’t solve the trouble by the supplied methods, please contact with the sales department of our company.
  • Page 26 phase phase contrast Use a the culture dish contrast effect observation, bottom whose bottom thickness is thickness of the culture dish less than 1.2mm is more than 1.2mm. Change to the phase contrast Use a bright field objective objective The condenser ring is not Adjust the condenser ring to coincident with the objective match the objective phase...
  • Page 27 DI-100F SOLUTION PROBLEM REASON PAGE II、Mechanical Part: The tension adjustment 1.The coarse focus Loose properly collar is too tight knob is hard to run 2.The image can’t stay on the focal The tension adjustment Tighten properly plane in the process...
  • Page 28 Adjusting the focus system, 1.the image make the double reticle and Focus incorrectly unfocused the specimen distinctly to The problem is unavoidable There is faintness It is a inherent character of if you used an achromatic around the image the achromatic objective objective 3.The indoor...
  • Page 29 Fax: 86-574-87229542 Authorized dealer for Nexcope line microscope in US: Ample Scientific L.L.C. Phone: (678)380-0369 Add: 4220 Steve Reynolds Boulevard, Suite 9 Norcross, GA 30093 Dana International Ltd. #1620,Samgu BLD,Hangangro-3Ga 16-49,Yongsan-Gu,Seoul,Korea . Postcode: 140-712 Phone: +82-2-6351-8161 Fax: +82-2-6351-8166 jsungk123@naver.com 담당 : 정성구 부장...

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