Taylor-Wharton TW-357 Operating And Maintenance Instruction Manual

Laboratory archival biological storage cryostorage system

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TW-357 REV E

LABS

(Laboratory Archival Biological Storage)

CRYOSTORAGE SYSTEM

OPERATING AND MAINTENANCE INSTRUCTION

REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN FORM # TW-10 P/N 7950-8052

BEFORE ATTEMPTING TO INSTALL, OPERATE OR PERFORM MAINTENANCE ON THIS

CRYOSTORAGE SYSTEM.

DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDERSTAND

THESE INSTRUCTIONS. DO NOT PERMIT UNTRAINED PERSONS TO USE OR

MAINTAIN THIS UNIT. IF YOU DO NOT FULLY UNDERSTAND THESE INSTRUCTIONS,

CONTACT YOUR SUPPLIER FOR FURTHER INFORMATION.

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Summary of Contents for Taylor-Wharton TW-357

Page 1 TW-357 REV E LABS (Laboratory Archival Biological Storage) CRYOSTORAGE SYSTEM OPERATING AND MAINTENANCE INSTRUCTION REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN FORM # TW-10 P/N 7950-8052 BEFORE ATTEMPTING TO INSTALL, OPERATE OR PERFORM MAINTENANCE ON THIS CRYOSTORAGE SYSTEM. DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDERSTAND THESE INSTRUCTIONS.
Page 2 TW-357 REV E...
Page 3: Table Of Contents
Control On Battery Backup Version Solenoid Valve INTERFACE SOFTWARE MAINTENANCE Strainer Cleaning Instructions Defrosting Your LAB-Series CryoStorage System Cleaning Your Taylor-Wharton CryoStorage System Making Adjustments to the LABS KRYOS Sensor Assembly Removing/Installing the Solenoid Valve Sensor Plug Schematic LABS Plumbing Assembly TROUBLESHOOTING...
Page 4: Warning
TW-357 REV E WARNING The following safety precautions are for your protection. Before installing, operating, or maintaining this unit read and follow all safety precautions in this section and in reference publications. Failure to observe all safety precautions can result in property damage, personal injury, or possibly death.
Page 5 Any Freight damage claims are your responsibility. Cryostorage systems are delivered to your carrier from Taylor-Wharton’s dock in new condition. When you receive our product you may expect it to be in that same condition. For your own protection, take time to visually inspect each shipment in the presence of the carrier’s agent before you accept delivery.
Page 6: General Information
• Specific Menu Descriptions under a main category are listed in Italics. Taylor-Wharton LABS CryoStorage Systems are designed for applications where extremely low temperature storage of biological products is required. They are also appropriate for industrial or other applications where liquid nitrogen temperatures and high capacity are needed.
Page 7: Labs Cryostorage Specifications
LABS Cryostorage Specifications Liquid nitrogen at atmospheric pressure weighs 1.78 lb./liter (0.8 kg/liter). All Taylor-Wharton Gas Equipment cryostorage systems are designed to support the full weight of liquid nitrogen and a complete stainless steel or aluminum inventory control system with boxes and specimens.
Page 8 Diagnostics: Temp. Calibration: Communications: Logging Capacity: Battery: Designed exclusively for all Taylor-Wharton LABS CryoStorage Systems 16.5 VAC, 40 Voltamps – Standard Battery Backup Version 24 VAC, 40 Voltamps (No Battery) (For power outlets other than North American 110 VAC contact Taylor-Wharton Customer Service.)
Page 9: Installation
If there is damage to the system during transit, be sure to file proper claims promptly with the carrier and insurance company. Please advise Taylor-Wharton of such filings. In case of parts or accessory shortages, advise Taylor-Wharton immediately. Taylor-Wharton cannot be responsible for any missing parts unless notified within 60 days of shipment.
Page 10: Power Supply Connection
Measure and make note of the “Start Fill”, “Stop Fill” and temperature sensors from the bottom of the appropriate sensor tube. You will need this information to set up the new controller. Remove old sensor wires. Calibrate new KRYOS control to new temperature sensor. Reverse procedure to install KRYOS control. TW-357 REV E...
Page 11: Operation
TW-357 REV E OPERATION These instructions are for operators experienced with cryogenic equipment. Before operating the system, become familiar with the safety precautions in this manual and in reference publications. Make certain all applicable provisions set forth in the Installation Section have been followed before placing a system in operation.
Page 12: Control Components
TW-357 REV E Control Components Interface Panel The KRYOS Interface panel, which the user will interact with, contains the vacuum fluorescent display as well as the number keypad, power button, help button and the soft-key control buttons. (See figure 1.0 below.) Figure 1.0 KRYOS Interface Panel...
Page 13 If your power source differs, or is subject to disruption or line surges due to other equipment on line, consult your Taylor- Wharton representative.
Page 14: Operating Parameters
On the interface panel, push (MENU, 2, 1). Insert the “Start Fill”, “Stop Fill” (Thermistor) sensors until the #6 sensor changes on the display from G (gas) to L (liquid). Return to the main (MENU). Dress wires and seal with stopper. TW-357 REV E...
Page 15: Adding An Inventory Control System
Always wear gloves when handling ICS racks or stored product, as they are very cold – read the precautions in the Safety section of these instructions, and in Taylor-Wharton publication TW-10 “Handle With Care”, for more detail on handling product stored in liquid nitrogen.
Page 16 81 Vial Polycarbonate, Cardboard, or Stainless Steel Boxes LABS-20K Racks BOXES-100 13 x 2 x 25 N-374187-92 R10K-9C44 (Case of 24 Polycarbonate) Figure 5.0 Vial Storage on Racks TW-357 REV E LABS-40K BOXES-25 VIAL N-374180 CAPACITY (Case of 36 Max. allowable Polycarbonate) 79300 41600...
Page 17 Blood Bag Storage in Cassette LABS-20K LABS-80K Fenwal 4R9951 Blood Bags – ask about other Bag types PART NUMBER CAPACITY LABS-80K 2100 LABS-40K 1204 LABS-20K Figure 6.0 Fenwal 4R59951 Blood Bag Rack Arrangements LABS-40K FRAMES CASSETTES 2100 1204 TW-357 REV E...
Page 18 Blood Bag Cassette Blood Bag Storage in Cassette LABS-20K LABS-80K Fenwal 4R9953 Blood Bags – ask about other Bag types PART NUMBER CAPACITY LABS-80K 1505 LABS-40K LABS-20K Figure 7.0 Fenwal 4R59953 Blood Bag Rack Arrangements LABS-40K FRAMES CASSETTES 1505 TW-357 REV E...
Page 19 Blood Bag Storage in Cassette LABS-80K Fenwal 4R5462 – 9955 Blood Bags – ask about other Bag types PART NUMBER CAPACITY LABS-80K 1050 LABS-40K LABS-20K Figure 8.0 Fenwal 4R5462 – 9955 Blood Bag Rack Arrangements LABS-20K LABS-40K FRAMES CASSETTES TW-357 REV E 1050...
Page 20: Control Operation
TW-357 REV E Control Operation This section of the operating manual is for Taylor-Wharton approved equipment that uses the KRYOS controller. Introduction The KRYOS temperature and LN2 level controller is designed for easy operation and uninterrupted, reliable service. This controller will maintain the selected temperature and liquid level range of LN2 in your refrigerator as well as provide audible and visual alarms for any alarm conditions that may occur.
Page 21 TW-357 REV E Main Display Screen The main display screen consists of 4 lines of information. Line 1: Displays the current status of the control. It indicates if all systems are normal or if any errors have been detected. Error messages disappear when the error is corrected.
Page 22 6.1 RS-232 Settings 6.2 Control Options 6.3 Display Brightness Action 6.4 About this Control TW-357 REV E 4.4.1 Thermocouple #1 Log Rate 4.4.2 Thermocouple #2 Log Rate 4.5.1 Erase System Logs 4.5.2 Erase Error Logs 4.5.3 Erase Temperature Log #1 4.5.4 Erase Temperature Log #2...
Page 23 TW-357 REV E Help Screens The Help button provides help to the user at any point in the menu system. The help message is displayed and the user is then prompted to press a button to return to the menu system.
Page 24 TW-357 REV E Sensor Type The sensor type can be set through the menu system (MENU, 2, 4). The sensor type selection should match the sensor type that is being used in the system. This is a 4-sensor, a 7-sensor (Freeze-Guard) or an 8-sensor array.
Page 25 TW-357 REV E System Alarms The Low LN2 Supply Alarm can be set through the menu system (MENU, 3, 3, 1). This alarm is activated if the solenoid valve is not closed within a designated time period after a fill starts. The solenoid valve can be closed either automatically (the LN2 level reaches the STOP FILL sensor) or manually (the stop fill button is pressed) to stop the timer which activates this alarm.
Page 26 REMOTE ALARM LOGGING is accessible through the menu system of the control (MENU, 4, 2, 3). The menu choice turns on/off the logging of the remote alarm activation. The choices are ENABLE OR DISABLE. Records data in the error log. TW-357 REV E...
Page 27 TW-357 REV E OPEN THERMOCOUPLE LOGGING is accessible through the menu system of the control (MENU, 4, 2, 4). This menu choice turns on/off the logging of the thermocouple open alarm. The choices are ENABLE and DISABLE. Records data in the error log.
Page 28 TW-357 REV E The MENU PASSWORD can be set through the menu system of the control (MENU, 5, 2). The menu password requires entry of a 4-digit password before allowing access to the menu system to change any control settings. Follow the directions on the display to set a new password or to disable a password.
Page 29 9999 when asked for the password. The KRYOS will display an 8- digit number. Call your distributor or Taylor-Wharton with the 8-digit number to obtain a unique 8-digit number to type into the control. When this number is entered, all passwords are cleared.
Page 30 TW-357 REV E Control Range CONTROL RANGE can be accessed through the menu system of the control (MENU, 6, 2, 3, 2, 2). KRYOS maintains a temperature range around the user-selected temperature. A tighter range maintains a temperature very close to the selected temperature at the cost of greater LN2 usage. A broad range provides more temperature variability but the LN2 usage is less.
Page 31: Control On Battery Backup Version
72 hours of continuous service without AC power. The system is designed for full operational service during a power failure, including full solenoid valve operation. The battery backup system can be ordered from Taylor-Wharton and should be factory installed. Differences between the regular Kryos and the battery backed Kryos: Please see the chart below for the differences between the two systems.
Page 32: Interface Software
Contact Taylor-Wharton for details. Please have your Cryostorage System serial number, KRYOS serial number and version number as it appears in ABOUT THIS CONTROL (MENU, 6, 4).
Page 33: Maintenance
A flow rate of 5 to 10 SCFM is sufficient to prevent water from collecting inside the unit. This flow of purge gas will also speed the warming of the unit to ambient temperature. TW-357 REV E...
Page 34: Cleaning Your Taylor-Wharton Cryostorage System
The vessel must be defrosted, cleaned, and sterilized regardless of the type of stored product, prior to return to Taylor-Wharton for repair or maintenance. Prior to cleaning, the unit must be warmed and defrosted following the defrosting procedure described above.
Page 35: Making Adjustments To The Labs Kryos Sensor Assembly
3/8 in. (9.53 mm) per day. If your measurement indicates a drop in excess of 3/4 in. (19 mm) per day, please contact your Taylor-Wharton distributor or Taylor-Wharton for further information. Please have your serial number, this manual and service history available.
Page 36 (P/N: EPROM-Kryos40) 2. The optional 4-thermistor sensor can be purchased from Taylor-Wharton to work with the Kryos control (works in either version of the software). This sensor allows the user to physically place the start fill and stop fill sensor at the appropriate level locations in the refrigerator.
Page 37: Removing/Installing The Solenoid Valve
Sensor Probes. The sensor probes used to detect liquid level by Taylor-Wharton controllers are temperature- sensitive resistors called “thermistors.” Their resistance to electrical current flow changes greatly with their temperature.
Page 38: Sensor Plug Schematic
TW-357 REV E Sensor Plug Schematic Figure 9.0 Pin View Schematics...
Page 39: Labs Plumbing Assembly
LABS Plumbing Assembly Freeze-Guard Sensor Male Branch 90 deg Elbow Solenoid Valve 90 deg Elbow Male Branch Reducer Strainer Female Connector Figure 10.0 Plumbing Assembly TW-357 REV E Relief Valve Tube Relief Valve...
Page 40: Troubleshooting
• Press FILL button to re-open fill solenoid valve. • Continue to lower the sensor until the STOP FILL thermistor is immersed in the LN2 . The fill should stop after a confirming (CHECK) SPLASH GUARD period. TW-357 REV E...
Page 41 TEMPERATURE. Follow the on-screen instructions. Hold the thermocouple in each bath until the control completes its self-calibration. • Make sure the thermocouple is clean and dry before and after each bath. • Reposition the thermocouple at the elevation the customer wants to monitor or control. TW-357 REV E...
Page 42 2. The switch can be bumped or moved during shipment. Adjust switch up until closed hinge will actuate switch lever. QCF (Quick Chill Feature) Will Not Operate See LID OPEN ALARM To activate or adjust times: Select MENU, USER OPTIONS, CONTROL OPTIONS, LID/DEFOG SETTINGS, QUICK CHILL. TW-357 REV E...
Page 43 SETTINGS, AUTO DE-FOG. Push Buttons Will Not Respond 1. Check all ribbon cable connectors on back of display panel. 2. Moisture may have migrated into the sealed faceplate. Call Taylor-Wharton for a Return Authorization. Liquid Level Readout is Incorrect Liquid level is determined by a seven-thermistor assembly inside a protective sensor tube. The user may adjust the desired START FILL elevations and STOP FILL elevations for the pool of nitrogen with the confines of the four inches.
Page 44: Factory Settings
Hi Temp #1 Log Enabled Hi Temp #2 Log Disabled Fill Logging Enabled Lid Open/Closed Log Enabled User Access Log Disabled Thermocouple #1 Rate Log Enabled Thermocouple #2 Rate Log Disabled Freeze-Guard: Valve Open Duration Disabled Valve De-icing Enabled TW-357 REV E...
Page 45: Replacement Parts
REPLACEMENT PARTS Refrigerator Parts (LABS) Lid Assembly……………………………………………………………LA80-9C85/LA40-9C85/LA20-9C85 Caster, ……………….. …………………………………………………………… 7300-8040 Decal, Warning……………………………………………………………………. R23K-9C42 Decal, Warning………………………………………………………………… Inventory Rack 25 Vials [13 shelves tall for 2 in (51mm.) boxes] (ea.)…………… R10K-9C44 Inventory Rack 100 Vials [13 shelves tall for 2 in (51mm.) boxes] (ea.) Level Controller Electrical/Mechanical Parts Controller KRYOS ……...…………………………………………………………...
Page 46 Date:_________________________________________________________________________________________ Date:_________________________________________________________________________________________ 1 All Taylor-Wharton CryoStorage Systems must be cleaned and sterilized prior to return to Taylor-Wharton for repair or maintenance and must be accompanied by a written statement to this effect. Any LABS-Service CryoStorage System received without this statement will be returned to the sender, freight collect. Contact Customer Service at 800-898-2657 for information.
Page 47 4075 Hamilton Blvd. Theodore, Alabama 36582 U.S.A. Telephone (251) 443-8680 Fax (251) 443-2250 In U.S. and Canada: (800) TW TANKS (898-2657) ©2002 Harsco Corporation...

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