Summary of Contents for Mascom MC513000
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Page 1: Operating Manual
Operating Manual GC-APPI Inferface for: Orbitrap Fusion Tribrid and Orbitrap Fusion Lumos Tribrid P/N: MC513000 Revision B... - Page 2 GC-APPI-Interface © 2018 MasCom Technologies GmbH Original Operating Instructions The manual describes the installation and operation of the GC-APPI-Interface for the Orbitrap Fusion series MS systems. Revision History: Revision A: published in December 2018 Revision B: published in May 2019 MasCom Technologies GmbH Sophie-Germain-Str.
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Page 3: Safety Instructions
We also recommend watching the GC-APPI- Interface installation video to familiarize yourself with the procedure. This video has been provided on a USB stick with the interface. Alternatively, you can download it using the following link: http://www.mascom- bremen.de/html/en/downloads/gc-appi- information GC-APPI Interface Page 3... - Page 4 Important information Any changes or modification to the GC-APPI interface, undertaken without our consent, may endanger the functionality and/or safety, and will result in the termination of the declaration of conformity. Tools required for source switching Changing from ESI to GC-APPI on the Exactive or LTQ Orbitrap series instruments only requires a few tools: Figure A: Screwdriver for removing the spray cone...
- Page 5 Figure D: Special tool for removing the transfer tube on Obritrap Fusion only Tools used for the GC column connection on the GC injector are described in the GC manual. GC-APPI Interface Page 5...
- Page 6 Installation of the GC-APPI Interface Before installing the GC-APPI-Interface you have to remove the ESI sprayer. Make sure the mass spectrometer is set to “Off”, and the heated ion transfer tube is set to room temperature (see Figure 1 and 4). Caution: Removing the ESI sprayer while the instrument is on or in standby will load the default Tune file and eventually start the capillary heating.
- Page 7 Carefully remove the ESI sprayer from the mass spectrometer interface and put it aside in a safe place. Remove the Thermo ion sweep cone as shown in Figure 3, which is the removable spray shield that sits just over the ion transfer tube. Using a small flat-head screwdriver, loosen the set screws on the sides of the spray shield.
- Page 8 The next step (Figure 5 and 6) is for Orbitrap Fusion Lumos only: Place the Vespel disk (P/N: MC502620) on the original ion transfer tube (no need to change it as on the Orbitrap Fusion) with the rounded edge towards the APPI-Interface (see Figure 6). Figure 5: Ion source without sweep cone Figure 6: Ion transfer tube with Vespel disk The next steps (Figure 5x, 6x and 7x)
- Page 9 Figure 5x: Special tool for transfer tube removal Figure 6x: Standard (A) and GC-APPI (B) transfer tubes (P/N: MC501730) (see Figure 5x) or with the 5/16 wrench as described in the operating manual of the mass spectrometer. Caution: Exchanging the transfer tubes at high temperature is difficult and may damage them.
- Page 10 All followings steps are for both Fusion type systems Bring the closed GC-APPI Interface in front of the Mass Spectrometer and arrange the cable for the APPI lamp close to its final position. Mount the interface, open it and lock it (Figure 7a, 7b, 8a and 8b) with a 6 mm Allen wrench.
- Page 11 Figure 8b: Mounting of the GC-APPI-Interface Next, attach the transfer line to the APPI-source. Remove the APPI lamp for better access to the transfer line connection. Place the connector in front of the transfer line (Figure 10) and after inserting the GC column place the graphite ferrule with the conical side towards the transfer line.
- Page 12 Attach the transfer line with connector and ferrule to the APPI source and only slightly tighten the connector (see Figure 12). Figure 12: Transfer line connection to APPI source Adjust the GC column before finally tighten the connector. It just has to protrude out of the inner source (see Figure 13a and b) by about 0.5 to 1 mm (check with a flashlight).
- Page 13 Finally tighten the transfer line connector and press the transfer line against the connector before fixing it with the clamp (see Figure 15). Figure 14: Transfer line in its final position Now insert the APPI lamp into the lamp holder and tighten the two screws to fix the lamp in its final position.
- Page 14 Figure 17: Fixing APPI lamp in its final position When the lamp is inserted and fixed, connect the cable to the lamp and fix the plug with the screws. Figure 18: Connecting the APPI lamp cable. Figure 19: Fixing APPI cable. Close the cover of the interface and make sure the protection shield completely covers the transfer line.
- Page 15 Figure 20: Closed APPI interface with dopant gas line connected In case you want to use an optional dopant gas, remove the blind plug and connect the dopant gas line as seen in Figure 20. If you do not want to use any dopant gas, please check if the blind plug is properly tighten.
- Page 16 Operation of the GC-APPI Interface Setting up the Instrument Configuration for Thermo GC Systems Close all Xcalibur programs and open the Instrument Configuration. Make sure the GC devices are installed and select the Thermo Focus, Trace 2000, Trace Ultra or Trace 1310 GC from the list of GC devices.
- Page 17 Figure 23: Xcalibur Instrument Configuration with Orbitrap Fusion selected Change the Default Source Type to ‘ Dedicated APCI’ and click on ‘ Apply’ . Leave this page with ‘ OK’ (see Figure 24). Figure 24: Xcalibur Instrument Configuration for Orbitrap Fusion Close the Instrument Configuration, start Xcalibur and wait for all instruments to initialize and to become “Ready for download”.
- Page 18 Tune the MS Turn on the MS and tune the mass spectrometer in APPI mode. Make sure the APPI lamp switch is in on position (blue LED is on, see Figure 25). Figure 25: APPI source with lamp switch If the APPI lamp is off, the noise level will be very high (see Figure 26).
- Page 19 Figure 27: Example of MS tuning in APPI mode Setup the Instrument Method The following is valid when using the Thermo Scientific Trace 1310 GC. For any other GC, we refer to its dedicated manual. Figure 28: Instrument Method Setup Open the Instrument Method editor from the Xcalibur Home page.
- Page 20 should not differ a lot from each other. Typical temperatures are between 225 and 300 deg. C. Please note: The maximum temperature of the APPI source is 330 deg. C. For details of the GC setup and/or programming, we refer to the GC Manual. Figure 29: Aux Temperature for Trace 1310 GC in Instrument Method Setup Switch to the MS to select or setup the...
- Page 21 Figure 30: “Change Instruments in Use” of Xcalibur Once you have setup the GC and autosampler as new devices in the instrument method, Xcalibur will ask to “Change the Instruments in Use”. Figure 30 shows the setup for using an autosampler as start device.
- Page 22 Final Remarks When everything is in place and the communication cables connected, close Xcalibur before switching on the GC and optionally the autosampler. After starting Xcalibur, all devices will be initialized and finally “ready for download” as shown below. Figure 32 Xcalibur Home Page showing all devices “Ready for Download”...
- Page 23 Final Disposal In case you want to finally dispose the GC-APPI interface, you can return it to us with a corresponding declaration of decontamination (form can be downloaded on our web site). GC-APPI Interface Page 23...
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Page 24: Troubleshooting
Troubleshooting When the vacuum is not in the range of 10e-1 mBar (make sure the Vespel disk is in place, see Figure 6), the position of the interface to the MS might be wrong and needs to be re-adjusted. For that, you have to loosen the knurled screw Figure 33 Knurled screw, which fixes the interface adjustment (see Figure 33) and adjust the distance of the... - Page 25 Spare Part List The following spare parts are available: Part No. Description MC502240 – Window MC505000 – PID-Lamp, 10.6 eV Kr MC502610 – Vespel Disk for Orbitrap Fusion MC502620 – Vespel Disk for Orbitrap Fusion Lumos MC501730 – Transfer Tube for Orbitrap Fusion MC502350 –...
- Page 26 Optional Items for GC-APPI The following items are available as option: Pneumatic Table for GC or HPLC P/N: MC600200 - work surface size 60 x 80 cm - lift able columns - adjustable height from 76 to 121 cm - integrated 3 IEC power plugs - table on caster wheels for easy transport GC-APPI Interface Page 26...
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