1. Manuals
  2. Brands
  3. Kendro Manuals
  4. Washer
  5. Sorvall Cellwasher 2
  6. Operating instructions manual

Kendro Sorvall Cellwasher 2 Operating Instructions Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

SORVALL
®
Cellwasher 2
OPERATING
INSTRUCTIONS
PN 04505-32

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Sorvall Cellwasher 2 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for Kendro Sorvall Cellwasher 2

  • Page 1 SORVALL ® Cellwasher 2 OPERATING INSTRUCTIONS PN 04505-32...
  • Page 3 OPERATING INSTRUCTIONS SORVALL Cellwasher 2 ® Cell Washing System Kendro Laboratory Products Asheville, North Carolina U.S.A. PN 04505-32 Issued January 2005...
  • Page 4 The SORVALL Cellwasher 2 is intended to be used in strict accordance with The American Association of Blood Banks' guidelines defined in the most recent edition of the AABB Technical Manual (published by AABB Press.

  • Page 5: Important Safety Information

    The Cellwasher 2 can be damaged if connected to the wrong voltage. Check the voltage before plugging the instrument into a power source. Kendro is not responsible for incorrect installation. Always maintain the Cellwasher 2 in the recommended manner. See Chapter 4, Maintenance.

  • Page 6: Table Of Contents

    Table of Contents SORVALL ® Centrifuges Table of Contents Page Safety Information Page ....... . . iii Chapter 1.
  • Page 7 Cellwasher 2 Table of Contents APPENDIX Warranty Index Decontamination Certificates List of Illustrations Figure Page 2-1. Cellwasher 2 Tubing Diagram ....2-3 2-2. Stainless Steel Rotating Bowl Installation .

  • Page 9: Cellwasher 2 Description

    Kendro for assistance. In the United States, call Kendro toll-free 1-800-522-7746; outside the United ® States, contact the nearest Kendro office (see back cover) or your local representative for SORVALL ® product information is available on our internet web site at http://www.kendro.com products.

  • Page 10: Cellwasher 2 Specifications

    W A R N I N G tory procedures in handling and disposing of blood serum, and observe safety precautions and good laboratory practices. The SORVALL Cellwasher 2 is intended to be used in strict accor- dance with The American Association...
  • Page 11 Cellwasher 2 Description Time Display Accuracy: ......±3% (Manual Mode*) Noise Level: ..... . <60 dB** Rotor Radii: 9.43 cm maximum 6.015 cm average...

  • Page 12: Accessories

    ® Installation SORVALL Centrifuges Accessories The accessories listed in Table 1-1 are supplied with all Cellwasher 2 models. Table 1-1. Accessories Supplied Catalog Number Description 43260 Distributor Assembly – for 12 mm x 75 mm and 10 mm x 75 mm tubes 12977 Tubing Kit containing: 1- 255 mm (10 inch) long frosted Pump Tubing...

  • Page 13: Chapter 2. Installation

    If you find any damage, please report it immediately to the transportation company and file a damage claim, then notify Kendro. Check all packages received against the shipping list and, after unpacking, check the accessories received against Table 1-1 on page 1-4;...

  • Page 14: Environmental Conditions

    ® Installation SORVALL Centrifuges Environmental Conditions An ambient temperature range of 10°C to 38°C (50°F to 100°F) should be maintained for Cellwasher 2 operation. The relative hu- midity can be up to 90%, non-condensing. The Cellwasher 2 is intended for use in 1) a Pollution Degree 2 Environment, 2) an installation category II supply circuit, and 3) at a maximum altitude of 2000 meters.

  • Page 15: Cellwasher 2 Tubing Diagram

    Cellwasher 2 Installation FLOW CONTROL VALVE SALINE PUMP HOUSING THUMBSCREW WASTE Figure 2-1. Cellwasher 2 Tubing Diagram Table 2-1. Parts Location: Tubing Diagram (keyed to figure 2-1) Item Description Overflow Tubing (from overflow tray), 150 mm (6 inch) Vent Tubing, 150 mm (6 inch) Pump Tubing, 255 mm (10 inch), frosted Flow Tubing (to nozzle), 305 mm (12 inch) Discharge Tubing (from collector ring), 200 mm (8 inch)
  • Page 16 ® Installation SORVALL Centrifuges 1. Unscrew the four knurled thumbscrews from the peristaltic pump, and remove the front half of the pump housing (do not remove the pump roller assembly). 2. Turn the roller assembly so that one of the rollers is in the 11 o'clock position.

  • Page 17: Rotor And Distributor Installation

    Cellwasher 2 Installation 11. Ideally, the drain tubing should be positioned so that it can not become immersed in collected waste fluid. However, if there is a possibility that the drain tubing will become immersed in waste fluid, cut the drain tubing at a convenient point near the waste receptacle and insert the Y-Connector.

  • Page 18: Stainless Steel Rotating Bowl Installation

    ® Installation SORVALL Centrifuges MOTOR DRIVE SHAFT SLOT ALIGNMENT STAINLESS STEEL ROTATING BOWL ASSEMBLY DRIVE PINS Figure 2-2. Stainless Steel Rotating Bowl Installation DISTRIBUTOR CANNULA DISTRIBUTOR ROTOR KNOB DA-12 ROTOR Figure 2-3. Distributor Installation...

  • Page 19: Rotor Loading And Balancing

    Cellwasher 2 Installation Rotor Loading and Balancing W A R N I N G Results may be affected if The Cellwasher 2 can process any balanced load up to twelve tubes. tubes are not within the specifica- When using less than a full complement of tubes, place the tubes in tions provided in Chapter 1 on page opposing compartments to ensure that the rotor is symmetrically 1-3.
  • Page 20 ® Installation SORVALL Centrifuges...

  • Page 21: Chapter 3. Operation

    W A R N I N G front panel keyboard, except for the main power switch and the remote saline flow control valve. The sensor-touch controls register The SORVALL Cellwasher 2 commands visually and/or audibly. is intended to be used in strict accor-...
  • Page 22 ® Operation SORVALL Centrifuges Table 3-1. Cellwasher 2 Controls and Indicators (continued) Item Name Acceptance Signal Function CYCLE Displays the number of wash cycles DISPLAY selected in the AUTO mode. TIME Displays the time selected for AUTO, DISPLAY HIGH, or LOW mode in seconds. TIME Digital time display, In HIGH or LOW mode only, selects...

  • Page 23: Principles Of Operation

    Cellwasher 2 Operation Figure 3-1. Cellwasher 2 Controls and Indicators Principles of Operation ® The SORVALL Cellwasher 2 has three modes of operation: AUTO, HIGH, and LOW. In the AUTO mode, the washing steps of the Coomb's Procedure are performed automatically. In the HIGH and LOW modes, the Cellwasher 2 spins at a fixed speed (see Chapter 1, Cellwasher 2 Specifications, page 1-2).
  • Page 24 ® Operation SORVALL Centrifuges The operator can interrupt the AUTO mode at any point to check the progress of the wash cycle by pressing the CHECK button. When the CHECK button is activated, the Cellwasher 2 will stop at the end of the washing step in progress (fill, spin, or decant).

  • Page 25: Tube Selection And Use

    Cellwasher 2 Operation Tube Selection and Use The Cellwasher 2 is designed to use either 5 ml 12 x 75 mm, or 3 ml 10 x 75 mm*, glass centrifuge tubes (saline flow must be adjusted to one size tube or the other). In normal operation, tubes are subjected to significant stress, making the use of high-strength centrifuge tubes necessary to avoid tube breakage.

  • Page 26: Priming The System & Adjusting Saline Fill Volume

    ® Operation SORVALL Centrifuges Priming the System & Adjusting Saline Fill Volume NOTE If sterilization is required, follow the Decontamination procedure in Chapter 4 on page 4-2, then prime the system as described below. The Cellwasher 2 must be primed and adjusted using the following procedure at installation, and whenever the saline supply reservoir or the tubing is changed.
  • Page 27 Cellwasher 2 Operation 7. Fine-adjust saline volume based on tube fills as follows: Fine-Adjusting Saline Fill Volume Based On Tube Fills This adjustment procedure is required when the system is initially set up, when priming the system, or whenever saline fill volume adjust- ments are required.

  • Page 28: Auto Operation

    ® Operation SORVALL Centrifuges Auto Operation W A R N I N G During operation, never a. The basic operating sequence in the AUTO mode is as follows: lean on or move the instrument, keep the defined clearance area clear of 1.
  • Page 29 Cellwasher 2 Operation 3. Close the lid and press START to continue the wash cycle in progress. NOTE The SALINE alarm may also indicate broken or ob- structed tubing. If the saline reservoir is adequate, check the tubing. Clean or replace tubing if necessary (see Chapter 4, Maintenance).

  • Page 30: High And Low Speed Operation

    ® Operation SORVALL Centrifuges High and Low Speed Operation W A R N I N G During operation, never a. To operate the Cellwasher 2 in the HIGH or LOW speed mode, lean on or move the instrument, keep proceed as follows: the defined clearance area clear of all objects (including all hazardous 1.

  • Page 31: Tube Breakage

    – if not, clear blockage and recheck as required. 4. Reinstall the wet guard, then, being sure to engage the drive pins on each, reinstall the rotating bowl assembly, the rotor, and the distributor assembly. If the problem recurs, contact Kendro Ser- vice. 3-11...

  • Page 32: Momentary Power Interruption

    ® Operation SORVALL Centrifuges Momentary Power Interruption W A R N I N G To avoid exposure to a If a momentary power interruption occurs when a run is in progress spinning rotor and potential for per- with the rotor spinning, it can take up to ten seconds before the rotor sonal injury, if a momentary power comes to a stop.

  • Page 33: Chapter 4. Maintenance

    It is also the responsibility of the user to make certain that these procedures are followed whenever necessary. If further ® service is needed, contact Kendro or your local representative for SORVALL products. Preventive Maintenance...
  • Page 34 ® Maintenance SORVALL Centrifuges b. Cleaning C A U T I O N • Clean the Cellwasher 2 cabinet with a damp cloth and mild detergent. All saline solutions have long term corrosive effects. Routine • Wipe up spills from the interior and exterior whenever they cleaning and maintenance are es- occur.

  • Page 35: Collecting Ring Assembly, Exploded View

    Cellwasher 2 Maintenance SPRAY NOZZLE COLLECTOR RING ASSEMBLY RETAINER O-RING RING GROOVE O-RING SEAL COLLECTOR RING STEP WASHER MOUNTING SCREW FLOW TUBING DISCHARGE TUBING TO FLOW DETECTOR TO WASTE CONTAINER OR T-CONNECTOR Figure 4-1. Collecting Ring Assembly, Exploded View c. Decontamination W A R N I N G The Cellwasher 2 is intended for use in Coomb's testing and incor- If tubes have been broken...
  • Page 36 ® Maintenance SORVALL Centrifuges If the Cellwasher 2 is operational 1. Prepare a 3-10% solution of household liquid chlorine bleach. 2. Soak a wash rag or sponge with the bleach solution and gently wipe the bowl assembly, the wet guard, and the inner portion of the Cellwasher 2, making sure all encrusted material is gone.

  • Page 37: Sevice Decontamination Policy

    1. Decontaminate the Cellwasher 2 to be serviced, and clean it of all encrusted material prior to servicing by a Kendro representa- tive or returning it to the Kendro facility. There must be no radioactivity detectable by survey equipment. Decontaminate...
  • Page 38 (Cellwasher 2), serial number and the repairs required. 2. Complete the Equipment Return Decontamination Form and return it to Kendro. Upon receipt of a completed form, a Re- turned Material Authorization Number (RMA Number) will be issued to you.

  • Page 39: Parts Replacement

    Cellwasher 2 Maintenance Parts Replacement To order replacement parts, telephone toll-free 800-522-7746 in the United States. Outside the United States, contact Kendro or your ® local representative for SORVALL products (see back cover for a partial list). Be prepared to supply the product name (Cellwasher 2), serial number, and a description of the parts required.
  • Page 40 Is saline volume correct? Saline Fill Volume Based on Tube Fills). Is distributor dirty? Are cannulas Clean distributor and each cannula. clogged? Are tubes the correct length and Contact Kendro Representative. diameter? Replace with tubes of correct length and diameter.
  • Page 41 Table 4-3. Troubleshooting Chart: Problem C Instrument will not operate and displays are illuminated. Is lid properly latched? Close lid properly. Contact Kendro Representative Table 4-4. Troubleshooting Chart: Problem D (see figures 4-2 and 4-3) Decant Malfunction. Is decant coil aligned properly?

  • Page 42: Location Of Decant Coil And Drive Ring

    ® Maintenance SORVALL Centrifuges MOTOR DRIVE SHAFT RING DECANT COIL Figure 4-2. Location of Decant Coil and Drive Ring BOWL LIFT PLATE RING Figure 4-3. Location of Lift Plate Ring 4-10...

  • Page 43: False Negative Results

    Repeat the test with fresh reagent. with human serum? 1. Check incubation time. Was cell-coating inadequate 2. Check the volume of during incubation? reagent added. Were the samples undercentrifuged Increase spin time. in the manual mode? Contact Kendro Representative 4-11...

  • Page 44: False Positive Results

    Test the patient serum for patient serum? bacterial contamination. 1. Test versus fresh reagent. Is extreme reticulocytosis 2. Observe patient red blood present? cells microscopically. Were the samples overcentrifuged in Decrease spin time. the manual mode? Contact Kendro Representative. 4-12...

  • Page 45: Inconsistent Reaction Strength

    Repeat the test, adding the Was the same amount of same amount of reagent to reagent added to each tube? each tube. Repeat the test with fresh Is the antiglobulin serum antiglobulin serum. weak or outdated? Contact Kendro Representative. 4-13/4-14...
  • Page 47 Cellwasher 2 Appendix APPENDIX...
  • Page 48 ® Appendix SORVALL Centrifuges Warranty Kendro Laboratory Products makes no warranty of any kind, expressed or implied, except as stated in this warranty policy. ® The SORVALL Cellwasher 2 Cell Washing instrument and DA-12 Rotor are warranted (subject to the conditions specified below and in...
  • Page 49 Cellwasher 2 Appendix Index Accessories Supplied, 1-4 Maintenance, Preventive, 4-1 Adjusting Fill Volume, 3-6 Applications Information, 1-1 Operation auto, 3-8 Balancing, Rotor, 2-7 high and low speed, 3-10 principles of, 3-3 Cellwasher 2 auto mode, 3-3 description, 1-1 high and low modes, 3-4 specifications, 1-2 Cleaning, 4-2 Parts Replacement, 4-7...
  • Page 50 NOTES...
  • Page 52 U.S.A. Fax: 1 (828) 665-4330 Tel.: 1 (800) 522-7746 e-mail: info@kendro.spx.com 1 (828) 658-2711 Fax: 1 (828) 665-4330 e-mail: info@kendro.spx.com Or contact a local representative for SORVALL ® brand products. Visit our web site at http://www.kendro.com or http://www.kendro.de Rev. 01/05...

Table of Contents