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For Detection and Enumeration of Contaminating Microbes
in Fuels, Lubricants and Water
Instructions for Use
Important Notice
Please read the Instructions for Use carefully before testing samples.
The test kit should only be used as part of an investigatory process into contamination in petroleum products and associated water. The
test kit must be used strictly in accordance with these instructions and/or Standard Test Method IP613 or ASTM D7978 or other instructions
authorised by ECHA Microbiology Ltd. The results only relate to the portion of sample tested and not necessarily to other petroleum product
in the system. Although guidance may be sought by the user on sampling and the interpretation of results, the responsibility for carrying out
the sampling and test procedures correctly is that of the end-user and not ECHA Microbiology Ltd. The test kit is designed to detect a
recognized group of micro-organisms of industrial significance, but it is in the nature of microbiology that there may be micro-organisms
present which are not detected by the test procedure. ECHA Microbiology Ltd does not accept any liability for any decision or assessment
taken or made as a consequence of the results obtained.
ECHA Microbiology Ltd, Units 22 & 23 Willowbrook Technology Park, Llandogo Road, St Mellons, Cardiff, UK, CF3 0EF
Tel: + 44 (0) 2920 365930
Fax: +44 (0) 2920 361195
sales@echamicrobiology.com
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T R U S T E D
I N N O V A T I V E
A C C U R A T E
D E P E N D A B L E
EP066.021216
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Table of Contents
Summary of Contents for echa MICROBMONITOR2
- Page 1 Although guidance may be sought by the user on sampling and the interpretation of results, the responsibility for carrying out the sampling and test procedures correctly is that of the end-user and not ECHA Microbiology Ltd. The test kit is designed to detect a recognized group of micro-organisms of industrial significance, but it is in the nature of microbiology that there may be micro-organisms present which are not detected by the test procedure.
- Page 2 The MicrobMonitor2 test consists of a screw-capped bottle containing a thixotropic nutrient gel. A sample is added to the gel using a sterile plastic loop or syringe (provided). The bottle is shaken and the gel liquefies and the sample, and any microorganisms in it are dispersed.
- Page 3 If the test is carried out more than 24 h after sampling, it is possible that the results will not reflect the viable microbial content present at the time of sampling. To avoid this, part or all of the MicrobMonitor2 test procedure can be conducted at the sampling location.
- Page 4 For consistency, it may be advisable to apply this settling time for all fuel samples even if free water is not observed. A.1.2 Remove the cap of the MicrobMonitor2 test bottle taking care not to touch the inside and place it on a clean surface.
- Page 5 Remove the loop from the sample and allow surplus fuel/oil to drain off; ensure a film of fuel/oil is trapped within the loop. A.2.5 Insert the loop into the neck of the MicrobMonitor2 test bottle and stab it into the gel and agitate it briefly to transfer the sample.
- Page 6 If possible swab a known area of surface. A.4.4 Insert the cotton end of the swab into the neck of the MicrobMonitor2 test bottle and stab it into the gel and agitate for about 15 seconds and then remove and discard.
- Page 7 Results that count When using MicrobMonitor2 to test for compliance with a specific industry standard (e.g. the IATA Contamination Limits shown in table 2 on page 8), if the test result indicates contamination is in the highest category (e.g. Heavy) before 4 days, it is generally acceptable to read and report the result without further incubation.
- Page 8 MICROBMONITOR2 ® Calculate the number of microbial CFU in the sample. For tests of fuel, numbers of microbes are conventionally expressed as number of CFU per litre. For tests of water phase or oil, numbers of microbes are conventionally expressed as number of CFU per ml.
- Page 9 MICROBMONITOR Test Results Chart ® Number of colonies in Microbial Content of Appearance after incubation test bottle Volume tested Sample (counted or estimated) 0.5 ml of fuel (syringe) <2000 CFU per litre 0.25 ml of fuel (syringe) <4000 CFU per litre None 0.1 ml of water (syringe) <10 CFU per ml...
- Page 10 STORAGE AND SHELF LIFE The shelf life of MicrobMonitor2 is 1 year from the date of production. The expiry date is printed on the batch label of each MicrobMonitor2 box. Store MicrobMonitor2 tests between 2 to 22°C, in the dark. The expiry date applies only to product stored as stipulated.
- Page 11 Results that count...
- Page 12 MICROBMONITOR2 ® T R U S T E D I N N O V A T I V E A C C U R A T E D E P E N D A B L E...
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Questions and answers
Hello, do you have the instuctions for use in french please ?