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Related Manuals for logos biosystems CELENA S
Summary of Contents for logos biosystems CELENA S
- Page 1 USER MANUAL LBSM-MD-ML-CLS-001 www.logosbio.com VC1712-01...
- Page 2 Do not disassemble the device on any occasion as this will invalidate your warranty. TRADEMARKS The trademarks used in this document are the property of Logos Biosystems, Inc. unless otherwise specified. LED Filter Cubes are LTC Licensed Products provided under an intellectual property license from Life Technologies Corporation.
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Page 3: Table Of Contents
Table of Contents Getting Started ...................................... 3 Basic Operation ....................................6 Imaging basics ......................................6 Iris diaphragm ....................................... 6 Phase annuli ......................................6 Imaging control panel ..................................7 Capture images in CAPTURE mode ..............................8 Image Editing and Analysis ................................. 9 CAPTURE control panel..................................... -
Page 4: Getting Started
Getting Started Product contents Product contents Your CELENA ® S is shipped with the following components: Filter cubes (as ordered) Objectives (as ordered) Insert plate Vessel holder frame Universal Holder 25 mm x 75 mm slide holder, two positions ... - Page 5 Setting up Unpacking ® Lift the CELENA S out of its box by grasping its base firmly with both hands at diagonally opposite ends. Place it on a clean, level, and sturdy surface. Do not hold or lift it by its top cover (where ®...
- Page 6 Insert the connector of the AC adapter into the power inlet of the instrument. Connect the power cord to the AC adapter. Connect the power cord to an electrical outlet. Make sure the power cords are appropriate for your region. Always use power cords and AC adapters provided by Logos Biosystems. CAMERA CABLE ®...
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Page 7: Basic Operation
Basic Operation Imaging basics Iris diaphragm Move the diaphragm slider to adjust the amount of transmitted light to reach the sample. Opening or closing the iris diaphragm regulates the resolution and contrast of an image. For transmitted light imaging, slide the diaphragm slider to the left. This opens the diaphragm to allow in the maximum amount of light and increase resolution. -
Page 8: Imaging Control Panel
Imaging control panel FILTER CUBES panel: The filter cubes panel shows the three interchangeable filter cubes for fluorescence imaging and one white filter cube for transmitted light imaging. The selected filter cube is highlighted in blue. Each filter cube can be renamed and its pseudocolor selected in the Settings. -
Page 9: Capture Images In Capture Mode
FOCUS panel: Use the focus panel to focus and to preset focus positions. The preset focusing speed and distance moved can be adjusted in the Settings. To focus, manually manipulate the focus knobs or use the sliders in the focus control panel. Drag the bars, click the arrowheads, or turn the mouse wheel while hovering over the sliders. -
Page 10: Image Editing And Analysis
Image Editing and Analysis CAPTURE control panel Images panel Use the IMAGES panel to adjust the brightness and contrast in each channel. To see the all the channels separately, click Tile. For a merged image of all the channels, click Overlay. In the Overlay view, click the thumbnail next to each channel to hide or show it in the merged image. -
Page 11: Save Images
Save images To save the image(s), click SAVE. In the save window, choose: File/folder name File path File type: TIFF, JPEG, BMP, or PNG To save as a folder Image color: Monochrome or pseudocolor Image type: single channels, overlay, annotated When Quick Save mode has been enabled, the CAPTURE button becomes the CAPTURE &... -
Page 12: Z-Stack Imaging
Z-stack Imaging Z-STACK mode Capture images in Click LIVE in the CAPTURE tab of the Z-STACK control panel. Z-STACK mode Set the iris diaphragm, phase annuli, objective, filter cube, light, and focus as needed. Select to set the start and end positions or to designate a range from the current position. -
Page 13: Process Images
Process images Select the PROCESSING tab in the Z-STACK control panel to add annotations to, create a slideshow video of, or merge the focus of Z-stack images. To add annotations: Load previously captured images by clicking LOAD IMAGES or use just captured images. Add a scale bar or the Z-position of the image as needed. -
Page 14: Time Lapse Imaging
Time Lapse Imaging TIME LAPSE mode Capturing time lapse Click LIVE in the CAPTURE tab of the TIME LAPSE control panel. images Set the diaphragm, phase annuli, objective, filter cube, light, and focus as needed. Set the total imaging time and the interval at which images should be captured. -
Page 15: Process Images
Process images Select the PROCESSING tab in the TIME LAPSE control panel to add annotations to or create a slideshow video of time lapse images. To add annotations: Load previously captured images by clicking LOAD IMAGES or use just captured images. Add a scale bar or timestamp as needed. -
Page 16: Cell Counting
Cell Counting CELL COUNTER mode provides the option to count cells automatically or manually. Both reusable and disposable slides from Logos Biosystems can be used for cell counting. AUTOMATED CELL COUNTER Automated cell counter Select the AUTOMATED tab in the CELL COUNTER control panel. -
Page 17: Counting Cells In Automated Cell Counting Mode
™ Counting Slides, PhotonSlides or LUNA Reusable Slides from Logos Biosystems. Focus in on the cell sample. Even with Autofocused Counting selected, the cells should be visible to ensure accurate focusing and counting. Adjust the light settings as needed or select Autoexposure. -
Page 18: Manual Cell Counter
To eject an external drive, select the drive you want to remove and click Eject USB at the bottom of the screen. Back up and/or transfer files from the onboard SSD periodically. Logos Biosystems does not warrant the data stored in the SSD and will not be held liable for damages associated with data... -
Page 19: Settings
Software update Download the most recent version of software from the Logos Biosystems website (www.logosbio.com) into the root directory of a USB drive. Insert the USB drive to a USB port in the instrument. Click S/W Update. Do not turn the instrument off during the update. -
Page 20: Maintenance
If liquid is spilled on the instrument, turn off the power and wipe dry immediately. Use only optical-grade cleaning materials to clean optical components. Do not exchange components between instruments unless they have been provided or authorized by Logos Biosystems. Installation and Objectives removal of Turn the instrument off and remove the insert plate from the stage. -
Page 21: Troubleshooting
Troubleshooting Image quality Misaligned overlay image Recapture images in each channel. Do not touch the stage or focusing knobs while capturing. Dim transmitted light image Check that the iris diaphragm and phase annuli are in the appropriate positions. Dim transmitted light image with TC PlanFluor objectives Set the phase annuli slider to the BF position. -
Page 22: Safety Information
Do not touch the instrument or its components with wet hands. Use components provided or authorized by Logos Biosystems. If the proper combination of components is not used, product safety cannot be guaranteed. Use only the provided power cord and AC adapter. If the proper components are not used, electrical safety of the product cannot be guaranteed. -
Page 23: Personal Safety
Community provisions for which this marking is required. Users must be aware of and follow the conditions described in this manual for operating the instrument. The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Logos Biosystems. Korean standards Symbol Description The KC certification mark indicates that this instrument conforms with Korea’s product... -
Page 24: Product Specifications
Product Specifications CELENA S Digital Imaging System ® Physical characteristics Instrument type Benchtop digital epifluorescence and transmitted light imaging system LCD display Full HD color LCD monitor, 1920 x 1080 pixels (not included) Dimensions 44 cm x 30 cm x 27 cm (17.3 in x 11.6 in x 10.6 in) Weight 20 kg (44 lb) Technical specifications... -
Page 25: Ordering Information
Ordering Information Instruments Cat # Product Quantity CS20001 CELENA ® S Digital Imaging System 1 set CELENA ® S Digital Imaging System Starter Kit - TC PlanAchro 4X Ph - DAPI CS20002 1 set - TC PlanFluor 10X - EGFP - TC PlanFluor 20X - RFP - TC PlanFluor 40X... - Page 26 Vessel holders Cat # Product Quantity I10201 Universal Holder 1 unit I10202 25 mm x 75 mm Slide Holder, Two Positions 1 unit I10203 35 mm Cell Culture Dish Holder, Four Positions 1 unit I10204 60 mm Cell Culture Dish Holder, Two Positions 1 unit I10205 100 mm Cell Culture Dish Holder, One Position...
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Page 27: Purchaser Notification
Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents owned or controlled by the Company which cover the product based on the manufacture, use or sale of a... - Page 28 Logos Biosystems HEADQUARTERS FL 2 & 3 28 Simindaero 327beon-gil, Dongan-gu Anyang-si, Gyeonggi-do 14055 OUTH OREA Tel: +82 31 478 4185 Fax: +82 31 478 4184 Email: sales@logosbio.com 7700 Little River Turnpike STE 207 Annandale, VA 22003 Tel: +1 703 622 4660...