Summary of Contents for Cell Guidance Systems Exo-spin Mini-Columns
- Page 1 User Guide Exo-spin™ Mini-Columns For rapid purification and clean up of samples up to 100 µl. Suitable for blood sera, cell culture medium and labeled exosome samples Cat EX03 Protocol Version 2.4...
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Page 2: Table Of Contents
Contents 1. Storage 2. Product Components 3. Product Information 4. General Information 5. Protocol for using (EX03) Exo-spin™ columns 6. Troubleshooting 7. Purchase Notification... -
Page 3: Storage
Storage Upon receipt, store at 4°C. Correctly stored components are stable for at least 6 months following purchase. Product Components EX03-8 • 8 x 1.0 ml Exo-spin™ columns and waste collection tubes EX03-25 • 24 x 1.0 ml Exo-spin™ columns and waste collection tubes EX03-50 •... -
Page 4: Product Information
Product Information Exo-spin™ columns may be used for isolation of intact exosomes from any sample smaller than 100 µl. This includes blood sera, but not blood plasma. Exo-spin™ columns may be used for purification of samples which have been purified by other methods, such as precipitation and ultracentrifugation, in cases where additional cleanup is required to remove proteins and smaller molecules. -
Page 5: Protocol For Using (Ex03) Exo-Spin™ Columns
Protocol for purification of intact exosomes using Exo-spin™ columns (EX03) Note Exo-spin™ columns are supplied pre-equilibrated with mili-Q water containing 20% ethanol. The column matrix should be re-equilibrated with PBS prior to use. A maximum of 100 µl per column may be used. A. - Page 6 Protocol A. For primary samples, such as sera, which contain cells, a step is required to remove cells and cell debris. This step may be omitted for secondary samples being cleaned up which do not contain cells. 1. Transfer 100 µl of starting sample to a centrifuge and spin at 300 × g for 10 minutes to remove cells.
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Page 7: Troubleshooting
Troubleshooting My sample does not elute from the column. Possible causes: Ensure that the plug has been removed from the base of column. If the column has been spun too fast, it will be compromised and subsequently not function correctly. Use our online tool and Nomograph, available on the product pages, to calculate the correct RPM for your centrifuge. -
Page 8: Purchase Notification
Purchaser Notification Limited warranty Cell Guidance Systems and/or its affiliate(s) warrant their products as set forth in the Terms of Sale found on the Cell Guidance Systems web site at www.cellgs.com/Pages/Terms_and_Conditions.html If you have any questions, please contact Cell Guidance Systems.