Summary of Contents for Cell biolabs ViraBind Series
- Page 1 Product Manual ViraBind™ AAV Purification Kit Catalog Number VPK-140 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures...
- Page 2 Recombinant AAV-2 is the most common serotype used in gene delivery, and can be produced at high titers with a helper virus or Cell Biolabs’ AAV Helper-Free System. AAV-2 can infect both dividing and non-dividing cells and can be maintained in the human host cell, creating the potential for long- term gene transfer.
- Page 3 Assay Principle...
- Page 4 Related Products 1. AAV-100: 293AAV Cell Line 2. VPK-141: ViraBind™ AAV Purification Mega Kit 3. VPK-145: QuickTiter™ AAV Quantitation Kit 4. AAV-200: ViraDuctin™ AAV Transduction Kit 5. VPK-099: ViraBind™ Adenovirus Miniprep Kit 6. VPK-100: ViraBind™ Adenovirus Purification Kit 7. VPK-104: ViraBind™ Lentivirus Purification Kit Kit Components ViraBind™...
- Page 5 The following procedure is suggested for one 15 cm dish or two 10 cm dishes and may be optimized to suit individual needs. Please refer to the user manual when using Cell Biolabs’ AAV Helper-Free System or other packaging systems.
- Page 6 7. Collect the AAV supernatant by centrifugation at 10,000 x g for 10 minutes. Discard the pellet. 8. Transfer the AAV supernatant to a clean 15 mL conical tube. 9. Resuspend the AAV Purification Matrix by inverting and shaking. Add 300 µL of matrix to the centrifuged supernatant.
- Page 7 Example of Results The following figures demonstrate typical purification results. One should use the data below for reference only. This data should not be used to interpret actual results. Figure 1: Purification of AAV2-GFP. AAV2-GFP was produced by a helper-free system in 293 cells.
- Page 8 to infect 293 cells, GFP positive cells were scored by counting after three days. A: AAV Supernatant; B: Flow through; C: 1 wash; D: Elution. Figure 2: Electrophoretic Profile of Purified AAV2-GFP. References 1. Rabinowitz, J, and Samulski, R. J. (1998) Curr. Opin. Biotechnol., 9, 470-475. 2.
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Page 9: Warranty
WARRANTY OF FITNESS FOR PARTICULAR PURPOSE. CELL BIOLABS’ sole obligation and purchaser’s exclusive remedy for breach of this warranty shall be, at the option of CELL BIOLABS, to repair or replace the products. In no event shall CELL BIOLABS be liable for any proximate, incidental or consequential damages in connection with the products.