Related Manuals for NanoEnTek ADAM-MC
Summary of Contents for NanoEnTek ADAM-MC
- Page 1 ADAM- ADAM A New Standard of A New Standard of Automatic Cell Counter Automatic Cell Counter INSTRUCTION MANUAL USER MANUAL NESMU-AMC-001E (V.5.5) NESMU-AMC-001E (V.5.1)
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Page 2: Table Of Contents
Maintenance and cleaning Software Installation 25 ~ 32 Introduction Getting started Installation 26 ~ 27 Function guide Function buttons Data list Information Graph Sample image of error message Trouble shooting Warranty Product list Purchaser notification ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 3: Product Contents
Main device User manual USB cable Installation CD Key pad Power cord Fuse External video monitor (Optional) The contents of the ADAM-MC‟s Accuchip Kit are listed below: Accuchip kit Accustain Accuchip2x Kit Accuchip4x Kit Solution Item (Cat. No: AD2K-200) (Cat. No: AD4K-200) (Cat. -
Page 4: Safety Information
Use this equipment only as specified in this manual and as specified in any documentation associated with its components. Any use of the equipment in an unspecified manner is strongly discouraged and may result in damage or injury as cautioned by signed warnings. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. - Page 5 Safety Information Section 3 , continued Safety The symbols used in the ADAM-MC and the manual are explained below: symbols The Caution symbol denotes a risk of safety hazard. ON (Power) Protective earth (Ground) The CE mark symbolizes that the product conforms to all applicable European Community provisions for which this marking is required.
- Page 6 • Do not reuse the Accuchips. Operator • Must have the general knowledge of cell counting procedure and bio safety to handle the sample that may have the infectious biohazardous substance. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
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Page 7: Product Specifications
AccuStain solution T,N PI (Propidium Iodide) staining of total cells (T) and non-viable cells (N). Accessories Power cord 1.5 m Fuse 250 VAC, 3 A; F3.15AL250V Keypad USB Type / 1.3 m ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 8: Description Of Adam-Mc
The ADAM-MC, an automated cell counter, is a benchtop automated cell counter that performs cell count and viability measurements of ADAM-MC using Accustain solution. The front view showing various parts of the ADAM-MC is shown below: Control Button Door Key Pad •... -
Page 9: Description Of Adam-Mc
Description of ADAM-MC , continued Section 1 Rear view The rear view showing various parts of the ADAM-MC. of ADAM-MC Key Pad Power Port Switch RS232C Video Power Port Port Port Plug Cooling fan Power switch Main power on/off switch... -
Page 10: Introduction 10
LED optics and CCD detection technologies to make the cell analysis more accurate and reliable. To count cells using ADAM-MC, the cells are mixed with a Propidium Iodide (PI) stain and directly pipetted on to a disposable plastic chip. The chip is then loaded onto a precision stage. -
Page 11: Basic Principals Of Counting
PI entering the cell. As a result, the nuclei of non-viable cells will only be stained. The ADAM-MC provides two kinds of staining solutions. AccuStain Solution T for the total cell counting is composed of the fluorescent dye (PI) and lysis solution. -
Page 12: Getting Started 12
1. Room temperature between 20 and 35 °C. Not recommended for cold room use (≤ 4 °C). • At low temperature (≤10 °C), warm up the ADAM-MC for 10 min. • 2. Not exposed to direct sun light. 3. Not subject to direct or continuous vibration.. -
Page 13: Installation
• Powering the instrument with an unapproved power cord may damaged the instrument. Plug to keypad. turn on the power switch. • Make sure that the main power switch is In the “Ⅰ” (ON) position. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 14: Start-Up Screen
System will go through self diagnostic tests. • If you get an error message, please contact your local distributor or sales@nanoentek.com. The home screens will be displayed as the image, no errors are detected. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 15: Error Message During Booting
It appears when turning off without locking device. Push the lock button before turn off the device. If this message still appears after restart, contact your local distributor or sales@nanoentek.com. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 16: Menu Setting
MENU, and Press the „Enter‟ key. The screen will return to the Menu [System Info.] screen automatically. Version : MC V1.21 Date : Sep 23, 11 CORE1 : 1.87 CORE2 : 1.52 CORE3 : 1.53 ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 17: Chip Selection
• „1‟ : Two channel chip (AccuChip • „2‟ : Four channel chip (AccuChip Press „1‟ or „2‟, and Enter key. After pressing the „Enter‟ key, the screen will return to the Menu screen automatically. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 18: Counting Method
: 30 frames capture High Precision: 22 frames capture High Speed : 11 frames capture Press „1‟ or „2‟ and „Enter‟ key. After pressing the „Enter‟ key, the screen will return to the Menu screen automatically. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 19: General Operation 19
Introduction Instruction is provided in this section for preparing the cell sample with Accustain solution for use with disposable Accuchip for automated cell count using the ADAM-MC. Preparing Cultivate the required number of cells. Add an appropriate volume of growth media or PBS to dilute cell to a final concentration of 5ⅹ10... -
Page 20: Operating Adam-Mc
☞ Note Automatic Focus will be carried out at the first time the device is booted. Once ADAM-MC has done the Auto Focus process and on the following time, focusing process will be skipped. The instrument takes approximately 90 seconds to count sample. -
Page 21: Icon Function
(After pressing the Eject button with AccuChip). Display menu setting Shows that performance setting is high precision. ADAM-MC scans 22 fields in each chamber, repres enting a total volume of 3.1 µ L. Shows that ADAM-MC reads 4 Channel chip. -
Page 22: Result Analysis
N1 (5.50E5) T2 (2.20E6) N2 (5.50E5) •The viability will be automatically calculated by the ADAM-MC software after each measurement of the total cells and the non-viable cells. •First, the total cell number and second, non-viable cell number were measured and then the cell viability is calculated as subtracting non-viable cell counting numbers from total cell counting. -
Page 23: Error Code
Cells are less than 4X10 cells/ml. “Low concentration cells” will be shown on Report program. Cells are less than 5X10 cells/ml. “Under detection range” will be shown on Report program. [ 4 channel ] ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 24: Maintenance And Cleaning
1. ADAM-MC does not need regular maintenance. and cleaning 2. ADAM-MC has no replacement of consumable materials. 3. Clean the exposed outer surface of ADAM-MC using a soft cloth and isopropyl alcohol or deionizers water. CAUTION: Dispose of wipes in an appropriately labeled solvent contaminated waste container. -
Page 25: Software Installation 25
All measurement results are saved on the memory of ADAM-MC, • automatically. User can download the data from the memory of ADAM-MC and export it to • Excel (*.xls) format. User can delete data from memory of ADAM-MC or can save captured •... - Page 26 Section 5 Software Installation , continued Installation To install the ADAM-MC Operation software, follow the directions as below: Insert the installation CD-ROM into the computer. Then open the file “Setup_ADAM_v1.x.x.x.exe”. ※Report program can be installed in Windows 2000, XP or higher version.
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Page 27: Installation 26
Initial installation folder is ☞ “C:\Program Files\Digital Bio\ADAM”. Report Program will be installed automatically. Click “Ok” to finish the installation. If the installation was successful, the report program can be found at Start>All Program>ADAM. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 28: Function Guide
Report Program are handled by using these buttons. (see p.29 for more detailed information of each button) ③ Data List All saved data in ADAM-MC will be loaded and shown in data list section. ④ Information Operation and counting results of each frame will be displayed here. -
Page 29: Function Buttons
, continued Function Start cell counting. buttons Park (Lock) stage of ADAM-MC. Eject chip holder out of ADAM-MC. Loads the experiment data from the memory of the main device. Deletes all of the loaded data and memory of the main device. -
Page 30: Data List
Up to 200 counting results are automatically saved to ADAM-MC memory. When memory of ADAM-MC is full, new counting result will replace old data. These data can be exported as Excel Sheet (*.xls) and stored in personal computer or can be erased from ADAM-MC memory. -
Page 31: Graph
Graph This section shows information of cell size distribution and counting results of each frame that ADAM-MC captured. Through cell size graph, you can figure out whether there are cell clumps or aggregates. In case of counting evenly distributed cells without any aggregation, there should be a single peak on distribution of cell size. -
Page 32: Sample Image Of Error Message
Software Installation , continued Sample image of error message ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 33: Trouble Shooting
(Error message: O) count again. • Low concentration of cells Check if concentration (Error message: L) of cell is too low. • Under detection range Use concentrated (Error message: U) sample and count again. ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 34: Warranty
Warranty Warranty NanoEnTek warrants that the ADAM-MC will be free from defects in material and workmanship for a period of one (1) year from date of purchase. If any defects occur in the ADAM-MC during this warranty period, NanoEnTek will repair or replace the defective parts at its discretion without charge. -
Page 35: Product List
T 12.5 ml AccuStain solution N 200 pcs AccuChip 12.5 ml AccuStain AD4K-200 AccuChip solution T 12.5 ml AccuStain solution N 12.5 ml AccuStain solution T ADR-1000 AccuStain 12.5 ml AccuStain solution N ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc. -
Page 36: Purchaser Notification
The information in this manual is described as correctly as possible and is applicable to the latest firmware and software versions, but it may be changed without prior consent or notification. Copyright 2008, by NanoEnTek Inc. All rights reserved. Published in Korea. Documentation : NESMU-AMC-001E (V.5.1) Revision History : V.0.0... - Page 37 Contact Information E-Mail : sales@nanoentek.com Website : www.nanoentek.com Developed and Manufactured by NanoEnTek Inc. NanoEnTek Inc. (HQ) 12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea Tel :+82-2-6220-7940 Fax:+82-2-6220-7721 NanoEnTek USA Inc. 5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA...