BIO RAD Bio-Plex Quick Manual

Bio-Plex Pro Human Immunotherapy Panel
Quick Guide
For Use with
Bio-Plex Pro Human Immunotherapy Assays
This guide can be used to prepare and run a full 1 x 96-well assay plate. New
users can download the complete manual, which includes detailed instructions
and a list of kit components, at bio-rad.com/bio-plex.
Initial Preparation
1. Plan the plate layout.
2. Start up/warm up the Bio-Plex Multiplex Immunoassay System (30 min).
Bring diluents, including wash buffer, assay buffer, standard
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diluent HB, detection antibody diluent HB, and sample diluent HB,
to room temperature (RT). Keep the other items on ice until needed
– Mix by inversion to ensure all salts are in solution
– Prepare 1x wash buffer: dilute 1 part 10x wash buffer (60 ml) with
9 parts distilled water (540 ml)
Begin to thaw the frozen samples
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3. Prepare the sample dilution according to the guidelines provided in the
following table. It is important to centrifuge serum or plasma samples at
1,000 x g for 15 min at 4°C to remove particulates from all samples prior to use.
Sample Type
Serum and plasma
Culture media and fluids
Note: ICAM-1 and VCAM-1 require higher dilution for serum and plasma (recommended 100-fold). Refer
to the Bio-Plex Pro Human Immunotherapy Assays Instruction Manual (#10000112563) for detailed sample
preparation recommendations.
4. Calibrate the Bio-Plex System within Bio-Plex Manager Software.
5. Reconstitute the standards and control by adding 250 µl of standard
diluent HB to each. Vortex at medium speed for 5 sec and incubate all
vials on ice for precisely 30 min.
Recommended Sample Dilution
1:4
User defined
Instruction Manual #
10000112563
Diluent
Sample diluent
Diluent + 0.5% bovine
serum albumin (BSA) (w/v)