The ST-200 aQua is an automated, microprocessor-controlled analyzer for measurement of sodium, potassium, ionized calcium, Lithium, Chloride & pH in serum, plasma, whole blood, diluted urine and cerebrospinal fluid. To obtain accurate results, the ST-200 aQua must be operated with Our Company’s specially...
Observe all warnings, notes and key information in this manual. Failure to leave the ST-200 aQua electrolyte analyzer to power with the REAGENT PACK in place could damage the electrodes and cause blockage. There are no operator serviceable parts inside the ST-200 aQua electrolyte analyzer.
Fluid Control Valve: Valve is mounted in ST-200 aQua for the supply of Calibrant A, Calibrant B solutions as per requirement. It controls the flow of Calibrant solutions by moving the lock pin.
ST-200 aQua Operator’s Manual b. Reference Housing: Remove the reference electrode from its package. Wipe the outside of the reference electrode to dry. Make sure all surfaces are clean and dry. Reference housing consists of a KCl solution. It comes with black lock on the seal to avoid transportation leakage of KCl Solution.
Stack the electrodes as shown in fig. Figure 5 ST-200 aQua Electrolyte analyzer comes with two blocks of 3 in 1 electrodes. The block 1 comes with two types of parameter combinations i.e; K, Na & K, Na, iCa and the block 2 comes with 8 types of parameter combinations as per the requirement (refer Figure 6).
Installation of tubing: The ST-200 aQua Electrolyte analyzers comes with two pump motors. The first pump motor is used for the flow of CAL-A & CAL-B solutions in to the electrode housing in control with the valve.
ST-200 aQua Operator’s Manual Figure 8 Pump tube installation procedure: 1. Module base: It is fixed to the chassis through a lock at the top of base as shown in the figure. We need to place the base bottom side first followed by the top, which consists of lock.
Make sure that the caps should be fixed and tighten properly. Do not use expired reagent pack. The electrode should be exposed to ST-200 aQua reagent all time so that it will protect electrode from getting damaged.
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ST-200 aQua Operator’s Manual Figure 9 Note: Don’t place the ST-200 aQua reagent packs open to atmosphere. Because, Only the small vent holes on the the solutions gets escape from the containers. caps should be open for air inlet as shown below figure.
ST-200 aQua Operator’s Manual C. Analyzer Restoration: To remove or replace any ST-200 aQua components, restore the ST-200 aQua in following order: Place the instrument into Maintenance mode. MENU 1.DIAGNOSTICS 2.USER MENU 3.SETUP MENU 4.PARAMETERS 5.SELECT iCa UNIT Press 1 ↓...
Other data can also be requested for printing. If required, the printer can be turned off. (To prevent damage to the printer mechanism, the printer must be turned off if the ST-200 aQua electrolyte analyzer runs out of printer paper.) Refer to the following precautions: In order to open the printer door lift the left side lock upward direction.
When all the components have been assembled, plug the ST-200 aQua electrolyte analyzer into a grounded outlet. When the ST-200 aQua electrolyte analyzer is plugged in, the power is ON. The Na/K/iCa/Li/pH/Cl analyzer will display,...
ST-200 aQua Operator’s Manual OPERATING THE ANALYZER 3. Operating the Analyzer A. Calibration The instrument has to be calibrated when it is switched ON or it automatically calibrates on set calibrated frequency after four hours or eight hours as per set frequency.
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ST-200 aQua Operator’s Manual PUMPING CAL A ↓ PUMP CAL A TEST Pump CAL = 1137 ↓ PUMPING AIR ↓ PUMPING CAL A To clean the electrodes ↓ CAL B MEASUREMENT ↓ … 3 cycles Air, Liquid Air, Liquid Air, Liquid...
ST-200 aQua Operator’s Manual ↓ CALIBRATION SLOPE Na = _______ _______ iCa = _______ Li = _______ pH = _______ Cl = _______ Finally the instrument displays calibration slope and is ready for analysis. E.g.: Calibration Slope Na = 67.10 K = 61.77...
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ST-200 aQua Operator’s Manual Once system is calibrated then it is ready to analyze patient samples, press Analyze key ANALYZE SAMPLE Y→ Continue N→ Exit Press Y ↓ is Sample Ready? Y→ Continue N→ Exit Take the sample by the pipette, drop it in the flow cell and Press Y ↓...
ST-200 aQua Operator’s Manual Na = 33.76 mV K = 49.54 mV iCa = 54.33 mV Li = 48.52 mV pH = -62.32 mV Cl = 49.73 mV ↓ ANALYZE SAMPLE Na = 140.9 mmol/L = 4.02 mmol/L iCa = 1.25 mmol/L = 1.15...
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ST-200 aQua Operator’s Manual Giving daily cleaner Figure 14 Press MENU MENU 1.DIAGNOSTICS 2.USER MENU 3.SETUP MENU 4.PARAMETERS 5.SELECT iCa UNIT Press 1 ↓ DIAGNOSTICS 1.DAILY CLEANER 2.MAINTENANCE 3.SENSORS 4.FLOW 5.HARDWARE 6.REAGENT PACK Press 1 ↓ DAILY CLEANER ↓ is Cleaner Ready? Y→...
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ST-200 aQua Operator’s Manual ↓ Aspirating Please Wait … ↓ CLEANING Performing… ↓ PUMPING AIR ↓ PUMPING CAL A ↓ PUMPING AIR ↓ Clean Cycle Performed ↓ PUMPING AIR ↓ PUMPING CAL A ↓ CAL B MEASUREMENT …….3 cycles of Cal B...
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ST-200 aQua Operator’s Manual Displays CAL A milli Volts ↓ Searching pack ↓ PUMPING AIR ↓ PUMPING CAL A… ↓ PUMP CAL A TEST Pump CAL = 1460 ↓ PUMPING AIR ↓ PUMPING CAL A To clean the electrodes ↓...
ST-200 aQua Operator’s Manual F. Analyze Urine To obtain urine sample, follow the procedure and precautions described under sample handling and collection. Dilute 200 microlitres of urine specimen with 1 mL (1000 microlitres) of urine diluent’s (this corresponds to 1 part urine and 5 parts urine diluent’s resulting in 1:5 dilutions).
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ST-200 aQua Operator’s Manual Aspirating urine: Figure 16 Set instrument in Urine type as follows: ANALYZE URINE Y→ Continue N→ Exit ↓ Press Y is Urine Ready? Y→ Continue N→ Exit Take the diluted urine solution by the pipette, drop it in the flow cell and Press Y ↓...
2. QC Results 3. Urine Results The ST-200 aQua has data storage and recovery capability of 2 Lakhs+ patients results, 25000 Urine results and 100 QC results. All results from the oldest day will be deleted when the storage capacity has been reached.
ST-200 aQua Operator’s Manual SEARCHING PATIENT ID: ST-200 aQua is having a feature to search patient results by entering Patient ID. User should enter patient ID (up to 5 numeric digits), after Pressing Y it will display results on screen, user can take print out by pressing Y button again.
Make sure that the caps should be fixed and tighten properly. Do not use expired reagent pack. The electrode should be exposed to ST-200 aQua reagent all time so that it will protect electrode from getting damage. Incompatible pack If unauthorized reagent pack is trying to use in analyzer then it will display Incompatible pack on screen.
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ST-200 aQua Operator’s Manual ↓ DIAGNOSTICS 1.DAILY CLEANER 2.MAINTENANCE 3.SENSORS 4.FLOW 5.HARDWARE 6.REAGENT PACK Press 1 ↓ DAILY CLEANER ↓ is Cleaner Ready? Y→ Continue N→ Exit ↓ Take the Cleaning Solution by the pipette and drop it in the flow cell and Press Y ↓...
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ST-200 aQua Operator’s Manual ↓ CAL A MEASUREMENT …..3 cycles of Cal A Air, Liquid Air, Liquid Air, Liquid ↓ Na = ______ mV K = ______ mV iCa= ______ mV Li = ______ mV pH = ______ mV Cl = ______ mV Displays CAL A milli Volts ↓...
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ST-200 aQua Operator’s Manual Na = ______ mV K = ______ mV iCa= ______ mV Li = ______ mV pH = ______ mV Cl = ______ mV Displays CAL A milli Volts on display ↓ CALIBRATION SLOPE Na = _______...
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ST-200 aQua Operator’s Manual ***MAINTENANCE *** N→ Exit Press N it will display ↓ EXITING MAINTENANCE Please wait … ↓ SENSACORE System Uncalibrated Please Calibrate Na* K* iCa* Li* pH* Cl* Press Calibrate Key. 3. SENSORS: Sensors is used to check mV of the electrodes for each reagent separately...
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ST-200 aQua Operator’s Manual … 3 cycles of Cal A Air, Liquid Air, Liquid Air, Liquid ↓ SENSOR CAL A-mV Na = ______ mV = ______ mV iCa = ______ mV Li = ______ mV pH = ______ mV Cl = ______ mV...
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ST-200 aQua Operator’s Manual DIAGNOSTICS 1.DAILY CLEANER 2.MAINTENANCE 3.SENSORS 4.FLOW 5.HARDWARE 6.REAGENT PACK Press 4 ↓ Reagent Priming FLOW 1.REAGENT PRIMING 2.PUMP CALIBRATION 3.VALVE TEST N→ Exit Press 1 ↓ REAGENT PRIMING 1.PRIME CAL A 2.PRIME CAL B 3.PRIME ALL N→...
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ST-200 aQua Operator’s Manual PRIMING CAL B Priming Completed N→ Exit ↓ Press N PRIMING CAL B Priming … ↓ REAGENT PRIMING 1.PRIME CAL A 2.PRIME CAL B 3.PRIME ALL N→ Exit Press 3 ↓ PRIMING CAL B Please wait …...
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ST-200 aQua Operator’s Manual ↓ PUMPING CAL A ↓ PUMP CAL A TEST Pump CAL = 1545 N→ Exit Instrument performs pump calibration using Cal A & displays the pump steps. VALVE TEST: VALVE TEST is used to clean the flow path of valve, by using DI water & syringe FLOW 1.REAGENT PRIMING...
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ST-200 aQua Operator’s Manual ↓ Press 2 CAL B FLOW ON N→ STOP Clean the CAL B flow path of valve before counter reaches ‘0’. ↓ Press N CAL B FLOW OFF N→ Exit Press N ↓ VALVE TEST 1.CAL A FLOW 2.CAL B FLOW...
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ST-200 aQua Operator’s Manual ↓ Press N AIR FLOW OFF N→ Exit 5. HARDWARE: MENU 1.DIAGNOSTICS 2.USER MENU 3.SETUP MENU 4.PARAMETERS 5.SELECT iCa UNIT Press 1 ↓ DIAGNOSTICS 1.DAILY CLEANER 2.MAINTENANCE 3.SENSORS 4.FLOW 5.HARDWARE 6.REAGENT PACK Press 5 ↓ Sample detector HARDWARE 1.SAMPLE DETECTOR...
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ST-200 aQua Operator’s Manual Pump module functionality: HARDWARE 1.SAMPLE DETECTOR 2.PUMP MODULE 3.SOFTWARE VERSION N→ Exit Press 2 ↓ PUMP SPEED → FAST ↓ PUMP SPEED → SLOW N→ Exit Both Pump rotates and checks its statuses. Software version: HARDWARE 1.SAMPLE DETECTOR...
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ST-200 aQua Operator’s Manual DIAGNOSTICS 1.DAILY CLEANER 2.MAINTENANCE 3.SENSORS 4.FLOW 5.HARDWARE 6.REAGENT PACK Press 6 ↓ REAGENT PACK 1.REAGENT PACK ID 2.REAGENT PACK INFO 3.REAGENT PACK CONSUMPTION Press 1 ↓ REAGENT PACK ID Please Wait… ↓ REAGENT PACK ID :BE91132C000007E0...
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ST-200 aQua Operator’s Manual CAL A CONCENTRATION Na : 148.0 4.10 iCa : 1.40 127.00 0.30 pH : 7.400 Concentration of CAL A is displayed. Press Y ↓ CAL B CONCENTRATION Na : 75.00 2.10 iCa : 2.90 60.00 2.00 pH : 6.78...
ST-200 aQua Operator’s Manual b) USER MENU Users menu permits the operator to check and test key operating functions of the ST-200 aQua. Following is the flow chart of users menu. MENU 1.DIAGNOSTICS 2.USER MENU 3.SETUP MENU 4.PARAMETERS 5.SELECT iCa UNIT Press 2 ↓...
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ST-200 aQua Operator’s Manual USER MENU 1.NORMAL RANGES 2.CALIBRATION FREQUENCY 3.SIPPING FREQUENCY 4.PRINT STORED RESULTS 5.AUTO-STANDBY 6.CORRECTION FACTOR Press 1 ↓ NORMAL RANGES 1.RESET RANGES 2.USER SETTINGS Press 2 ↓ USER SETTINGS 1.SAMPLE RANGES 2.URINE RANGES 3.QC1 RANGES 4.QC2 RANGES 5.QC3 RANGES...
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ST-200 aQua Operator’s Manual CALIBRATION FREQUENCY Instrument will perform automatic calibration for every 4 hours /8 hours for good performance of the instrument. USER MENU 1.NORMAL RANGES 2.CALIBRATION FREQUENCY 3.SIPPING FREQUENCY 4.PRINT STORED RESULTS 5.AUTO-STANDBY 6.CORRECTION FACTOR Press 2 ↓...
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ST-200 aQua Operator’s Manual PRINT RESULTS USER MENU 1.NORMAL RANGES 2.CALIBRATION FREQUENCY 3.SIPPING FREQUENCY 4.PRINT STORED RESULTS 5.AUTO STANDBY 6.CORRECTION FACTOR Press 4 PRINT STORED SAMPLE RESULTS: PRINT STORED RESULTS 1.Sample Results 2.QC Results 3.Urine Results Press 1 ↓ STORED SAMPLE RESULTS Please Enter ‘5’...
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ST-200 aQua Operator’s Manual PRINT STORED RESULTS 1.Sample Results 2.QC Results 3.Urine Results Press 2 ↓ -- QC Samples -- 1. Last 1-25 samples 2. Last 26-50 samples 3. Last 51-75 samples 4. Last 76-100 samples N→ Exit Depending upon the result required select the range option.
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ST-200 aQua Operator’s Manual RESULT : 02 133.54 4.129 iCa= 1.083 Cl = 94.645 0.00 7.09 Y→ Print N→ Next Press ‘ Y ‘ to print displayed result or press ‘ N ‘ to obtain next result. PRINT STORED URINE RESULTS: PRINT STORED RESULTS 1.Sample Results...
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ST-200 aQua Operator’s Manual MENU 1.DIAGNOSTICS 2.USER MENU 3.SETUP MENU 4.PARAMETERS 5.SELECT iCa UNIT Press 2 ↓ USER MENU 1.NORMAL RANGES 2.CALIBRATION FREQUENCY 3.SIPPING FREQUENCY 4.PRINT STORED RESULTS 5.AUTO-STANDBY 6.CORRECTION FACTOR Press 5 for Auto-Standby ↓ AUTO-STANDBY STATUS : ON Y→ON...
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ST-200 aQua Operator’s Manual SLOPE FACTOR: Slope factor is used for multiplying the factor with the result obtained. Figure 17 In the above graph, green line indicates the normal/reference state (All the control levels lie with its range as per the Q.C sheet provided in the Q.C box) and red line indicates the error/out-off state (Electrodes are reading either higher or lower).
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ST-200 aQua Operator’s Manual INTERCEPT: Intercept is used if all the three control values read either higher or lower. Figure 18 (i.e.) y = mx + c Where m is the slope of the line and C is the y-intercept, which is the y-coordinate of the location where line crosses the y axis.
ST-200 aQua Operator’s Manual SLOPE/INTERCEPT 1. Na 2. K 3. Cl 4. iCa 5. Li 6. pH C) Setup Menu Setup menu permits the operator to check and test key operating functions of the ST-200 aQua. Following is the flow chart of setup menu.
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ST-200 aQua Operator’s Manual ANALYZE TYPE URINE N→ Exit Press N ANALYZE TYPE 1.SERUM / PLASMA 2.URINE 3.WHOLE BLOOD 4.QUALITY CONTROL 5.NON DETECTABLE Press 3 ↓ ANALYZE TYPE WHOLE BLOOD N→ Exit Press N ANALYZE TYPE 1.SERUM / PLASMA 2.URINE 3.WHOLE BLOOD...
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ST-200 aQua Operator’s Manual Date / Time: By using this option user can change the Date & Time of the instrument. By using Y button we can select the field which we want to change & by using N button we can change the field.
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ST-200 aQua Operator’s Manual DATE/TIME DATE : June 23, 2017 TIME : 11:---:--- Y→ SET N→ NEXT Set the Time: Minutes Select Minute: Y→ SET DATE/TIME DATE : June 23, 2017 TIME : 11:47:--- Y→ SET N→ NEXT Set the Time: Seconds Select Seconds: Y→...
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ST-200 aQua Operator’s Manual Press 3 PRINTER STATUS : ON Y→ON N→OFF Press Y to ON, press N to OFF the printer status SETUP MENU 1.ANALYZE TYPE 2.DATE/TIME 3.PRINTER 4.PRINT-PARAMETERS 5.PRINT-mV 6.PATIENT-ID Press 4 ↓ PRINT PARAMETERS 1.Na 3.Cl 4.iCa 5.Li...
ST-200 aQua Operator’s Manual PATIENT ID: Patient ID should be ON to get previous stored results. If patient –ID option is deselected then instrument will not ask to enter patient ID while analyzing. SETUP MENU 1.ANALYZE TYPE 2.DATE/TIME 3.PRINTER 4.PRINT-PARAMETERS 5.PRINT-mV...
ST-200 aQua Operator’s Manual If your required model is present in this screen then select the model by pressing appropriate number, if not then press Y button to see more models 1. K Na iCa Li pH * 2. K Na iCa pH * 3.
ST-200 aQua Operator’s Manual HANDLING AND COLLECTION 4. Sample Handling and Collection Warning: Human body fluid specimens may be contaminated with HIV or other pathogens. Treat all specimens and collection devices and tools as biohazardous materials. A. Whole Blood Whole blood specimens should be drawn carefully to avoid hemolysis.
ST-200 aQua Operator’s Manual To obtain accurate results, samples should be free of any clots, fibrin, etc., which would obstruct sample flow and affect results. The use of a serum clearing agent is strongly recommended. If a serum separator tube is utilized, care must be taken to avoid inserting the sample probe into the gel layer.
ST-200 aQua Operator’s Manual E. Expected Values The values given in below tables (table 1 & 2) are intended to be used only as a guide. Each laboratory or testing site should establish its own range of normal values, taking into account factors such as age, sex, diet and other determinants of electrolyte levels.
ST-200 aQua Operator’s Manual MAINTENANCE 5. Maintenance The ST-200 aQua electrolyte analyzer requires very little operator maintenance. The only maintenance required is to run the daily cleaning solution every day mandatory. All other maintenance is performed by replacing the components as described in the schedule below. When you wish to perform routine or any other maintenance function, go to the MENU option and Select “Maintenance”.
These sensors are known as Ion selective electrodes. The ST-200 aQua electrolyte analyzer measures sodium, potassium, ionized calcium, lithium and chloride in biological fluids, using ion selective electrode technology. The flow-through sodium electrodes contain plasma tube, specially formulated to be sensitive to sodium ions.
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Cal A reading and the Cal B reading. A slope outside the built-in software limits will be flagged and displayed as low or high. The ST-200 aQua electrolyte analyzer will detect excessive drifts or noise and display the appropriate error message.
ST-200 aQua Operator’s Manual TROUBLESHOOTING 8. Troubleshooting Introduction To enhance trouble-free operation of the ST-200 aQua, it is imperative to follow maintenance schedule outlined under ST-200 aQua maintenance. When any error messages appear in the display, then refer to the list of “typical error messages”...
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ST-200 aQua Operator’s Manual Troubleshooting Kit Tools to help diagnose and correct problems: 10mL syringe - 01 Needles - 03 Test Tubes - 10 Fuse (2 amp) - 01 Fuse (5 amp) - 01 Electrode seals - 03 ...
ST-200 aQua Operator’s Manual A. Flow Path System All reagents and samples flow in one direction through the entire flow path system as shown. The flow path is a open loop with one valve, two pump motors and reference housing which ensures that the electrodes will always be kept wet, an important requirement of ISE’s.
If the solutions valve requires cleaning, the operator must remove all of the items in the above list, before removing the solutions valve. To install components on the ST-200 aQua, follow the installation order outlined under installation and replacement. Troubleshooting is covered in the order that problems are most likely to occur.
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ST-200 aQua Operator’s Manual block connectors or seals are properly installed. Performing this function twice may eliminate the error. FLOW ERROR (Valve fluidic error or Fluid path error): Flow error depends on following points. 1) Electrode housing. 2) Flow cell.
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ST-200 aQua Operator’s Manual Menu Press 1 ↓ Diagnostics Press 2 ↓ Maintenance Both pumps rotates. The liquid is aspirated through the first pump motor and it is removed from electrode housing by second pump motor. Check whether liquid is flowing properly or not. If not, flush or replace pump tubing.
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ST-200 aQua Operator’s Manual Disconnect the inflow tube (from valve) and outflow tube (to flow cell) of first pump motor and also disconnect the inflow tube (from reference housing, red stopper) and outflow tube (to the waste container) of second pump motor.
C. Electrode Assembly The electrode block assembly should be replaced after 6 months of use. ST-200 aQua guarantees this item’s performance for 6 months of use, but the frequency of replacement may depend on your sample volume or sample type (volume of lipemic high protein samples may necessitate more frequent changes of the electrode block assembly).
The ST-200 aQua has an RFID (Radio Frequency Identification System) which keeps track of the reagent pack usage. The ST-200 aQua will indicate the availability of reagents in a decreasing manner i.e., 100% to 0% at every analysis.
Figure 20 Figure 21 F. Printer If the ST-200 aQua printer fails to print, attempt the following procedures: 1. To verify that the printer is turned on, enter the SETUP MENU and check PRINTER ON/OFF. 2. Check the printer mechanism for any paper obstructions in the printer head area.
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PRECISION 1 Collect 5 patient samples. 2 Analyze each sample 10 times consecutively on the ST-200 aQua and record the data. 3 Calculate the mean, standard deviation and C.V. for each sample. 4 Compare the C.V. for sodium, potassium and chloride and the S.D.
ST-200 aQua Operator’s Manual DISPLAY MESSAGES Display Messages ANALYZE SAMPLE Perform Whole heparin blood, serum, CSF Sample analysis. ANALYZE URINE Perform diluted urine Sample analysis. ANALYZE QC Perform QC sample analysis. AIR DETECTED Air detected in Sampler detector test. AIR NOT DETECTED Air not detected in Sampler detector test.
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ST-200 aQua Operator’s Manual INCOMPATIBLE PACK Pack is not suitable for the instrument. CLEANING… Analyzer is performing daily cleaning CLEANING CYCLE PERFORMED … Appears after daily cleaner process over. SYSTEM UNCALIBRATED PLEASE CALIBRATE Na/K/iCa/Cl/Li /pH HIGH… Na/K/iCa/Cl/Li /pH result obtained...
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ST-200 aQua Operator’s Manual LIQUID DETECTED Message will appear when the liquid is detected in sample detector test. LIQUID NOT DETECTED Message will appear when the liquid is not detected in sample detector test. LOT NO: Allows the operator to check lot number MENU Menu selection.
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ST-200 aQua Operator’s Manual PRINT STORED RESULTS Allows operator to display last sample results or print stored results PROBE IN SAMPLE Requests operator to confirm that probe is in sample. PROBE IN CLEANER Requests operator to confirm that probe is in Daily Cleaning Solution...
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ST-200 aQua Operator’s Manual NORMAL RANGES Used to set the Na/K/iCa/Cl/Li/pH sample limits ANALYZE TYPE Allows operator to select the sample type. Eg: SAMPLE/URINE/QC ST-200 aQua The first displayed message. SENSOR CAL A –mV Allows the operator to display the mV readings of CAL A reagent selected.
In the event of failure within the warranty period, ST-200 aQua will repair or replace any Analyzer returned. ST-200 aQua reserves the right to ask for proof of date of purchase, such as original invoice and / or maintenance records.
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ST-200 Auto Queue Universal Analyzer Plot No: 3, Export Promotion Industrial Park, Pashamylaram, Sangareddy (Dist), Hyderabad - 502307. Telangana, INDIA. Telephone : +08455 - 223400 web : www.sensacore.com...
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Questions and answers
AIR IN CALI B ERROR
NOT GETTING SODIUM RESULTS. GETTING VALUES OF REMAINING ELECTROLYTES