1. Manuals
  2. Brands
  3. Techcomp Manuals
  4. Measuring Instruments
  5. FL970
  6. Instruction manual

Techcomp FL970 Instruction Manual

Fluorescence spectrometer
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
INSTRUCTION
MANUAL
FL970
Fluorescence Spectrometer
FL970——Fluorescence Spectrometer
Edition：01
0

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the FL970 and is the answer not in the manual?

Questions and answers

Related Manuals for Techcomp FL970

Summary of Contents for Techcomp FL970

  • Page 1 INSTRUCTION MANUAL FL970 Fluorescence Spectrometer FL970——Fluorescence Spectrometer Edition：01...

  • Page 2: Table Of Contents

    Contents PREFACE ..........................1 ABOUT THIS MANUAL ......................1 Precautions on Electromagnetic Wave Interference ..............2 Warranty on Product ......................... 3 Technical Service on Installation、Relocation and After Sale ..........4 Technical Training ........................4 About Discard ........................... 4 Other Precautions ........................5 Safety Precautions ........................
  • Page 3 3.7.1 Parameters Setting ....................45 3.7.2 Sample Measurement ..................... 49 3.7.3 Data Processing and Print ................... 50 3.8 3D Scan .......................... 50 3.8.1 Parameters Setting ....................51 3.8.2 Execute ........................ 55 3.8.3 Stop ........................56 3.9 Quantitation Measurement ....................57 3.9.1 Parameters Setting ....................

  • Page 4: Preface

    ABOUT THIS MANUAL This instruction manual is used to guide the user’s operation of FL970 fluorescence spectrometer, the operation methods and safety cautions when operating the instrument are introduced, be sure to read through this manual before using the instrument.

  • Page 5: Precautions On Electromagnetic Wave Interference

    Precautions on Electromagnetic Wave Interference (1) Possible Electromagnetic Wave Interference Caused by this Instrument When this instrument is in use, it may cause electromagnetic wave interference to the other equipment, such as television and radio. The electromagnetic wave interference can be minimized by choosing the specific circuit connection cables.

  • Page 6: Warranty On Product

    Warranty on Product The FL970 fluorescence spectrometer is warranted to operate according to the specifications given in the instruction manual, provided it is used in accordance with the instructions described in the manual. (1) Scope of Warranty (a) Main unit of the instrument which prove to be defective in design or workmanship during the warranty period will be repaired without charge.

  • Page 7: Technical Service On Installation、Relocation And After Sale

    If relocation of the instrument becomes necessary after initial installation (delivery), please notify your local sales representative or nearest service office of Techcomp. Technical Training For using the instrument safely and correctly, technical training speeches are held at Techcomp’s or user’s site.

  • Page 8: Other Precautions

    (a) Information contained in this instruction manual furnished with the instrument is subjected to change without notice for product improvement. (b) The copyright of this manual belongs to the technical department of Techcomp. (c) No part of this manual may be reproduced or transmitted in any form or by any...

  • Page 9: Safety Precautions

    In Order To Operate Safely Safety Precautions Before using FL970 fluorescence spectrometer, please read the following safety instructions carefully and understand them fully. General Precautions on Safety ■ Following all the operation procedures provided in this instruction manual. ■ Installation and maintenance activities of this instrument are limited to the service personnel trained and qualified by Techcomp.
  • Page 10 In Order To Operate Safely ■ Please do not operate and use the instrument other than all contents recorded in the instruction manual. ■ If there are any problems of the instrument, please contact related distributors or our company’s technical service department. ■...
  • Page 11 In Order To Operate Safely This instruction manual contains the following important safety notices: Danger No such signs in this instrument. Warning Electric shock accident caused by power supply voltage (100V, 200V) ■ Power supply voltage of 198V～242V is supplied to this instrument, which may cause electric shock and death accident.
  • Page 12 Safety Precautions On FL970 Fluorescence Spectrometer About working power (a) The voltage for FL970 fluorescence spectrometer must be AC100V or AC220V (50Hz or 60Hz), and the power is over 1KVA. If the power supply is fluctuated or there is interference on the power supply line, which will not only affect the normal operation of the instrument, but also may cause accidents.
  • Page 13 containing viruses, as well as programs, software and electronic documents containing viruses when surfing the internet or communicating with others. Therefore, please be sure to confirm the programs, software and electronic documents that need to be used. Do not use the programs and software from unknown sources to avoid computer virus infection.
  • Page 14 In Order To Operate Safely About turning off computer power If turning off the computer together with the instrument which is still running, it may do a damage to the computer, or operation system, application software and data may be destroyed, or the data can’t be saved. Before turning off the computer, please exit the application software of the instrument, and then shut down the computer according to the correct steps in the operation system.

  • Page 15: Warning Labels

    In Order To Operate Safely Warning Labels The warning labels shown below are attached to FL970 fluorescence spectrometer. (1) Dangerous voltage (500V、220V) The warning label above is attached to the cover of the lamp source compartment and power supply socket (2) Attention to high temperature The warning label above is attached to the cover of the lamp source compartment.

  • Page 16: Social Responsibility Of Enterprise

    Shanghai Techcomp Instrument Limited is well aware of the importance of social responsibility to enterprise and it is the pillar for the sustainable development of the enterprise. In...

  • Page 17: Chapter 1 Installation

    If you need this service, please fill in the user contact card and the warranty card, and then send them back to Techcomp, so that we can arrange on-site service. Meanwhile, keep the instrument and accessories intact without power on or operate is. Please prepare the installation site and power supply for this instrument.

  • Page 18: Instrument Connection

    In order to full utilize the instrument and to keep performance for long term usage, please install the instrument in the sites that meet the following requirements. Techcomp and its representative will not be responsible for the performance deterioration and damage of the instrument due to operate the instrument in the sites that do not meet the specified requirements, even within the warranty period.

  • Page 19: Precautions On Using Workstation

    Games playing on this computer is not allowed. Otherwise, if the workstation is infected by computer viruses, the consequences will be unimaginable, and Techcomp and its representative is not responsible for the damage done by this cause, even within the warranty period.
  • Page 20 Figure 1-2 Remove the outer cover of the lamp compartment ② Figure 1-3 Pull out the connection line from the fan wire interface ④ ④ ④ ③ Figure 1-4 Remove the outer cover of the lamp compartment...
  • Page 21 Figure 1-5 Schematic diagram of xenon lamp profile Figure 1-6 Light source safety switch bar on the inner cover of the light source...
  • Page 22 Figure 1-7 Change the location of the light source safety switch Figure 1-8 Install the inner cover of the lamp compartment...

  • Page 23: Lamp Position Adjustment

    Attention Don’t pull the lamp by the positive wire when fixing the positive end with a nut. Be sure to reserve a natural bending section of the positive wire, if the positive wire is pulled too tightly, the xenon lamp will be at the risk of being damaged when lighting it. 1.6 Lamp Position Adjustment In order to optimize the performance of the instrument, please adjust the position of the lamp after the initial installation or replacement the lamp.
  • Page 24 Figure 1-10 Adjust the energy value of Raman peak to be maximum Techcomp and its representative is responsible for the installing and commissioning of FL970 fluorescence spectrometer. Techcomp and its representative is to open the cover of FL970 fluorescence spectrometer and adjust it accordingly before the instrument is handed over...

  • Page 25: Chapter 2 Function

    Chapter 2 Function 2.1 Optical System Figure 2-1 is the schematic diagram of FL970 optical system. The light source is 150W xenon lamp. Ozone generated is sealed by the structure of the light source chamber and can be decomposed by the heat generated. The bright spot of the xenon lamp is magnified by the ellipsoidal lens, focused and directed to the entrance slit on the excitation side by the dichroic.

  • Page 26: Specifications

    Figure 2-2 Schematic of FL970 2.3 Specifications 1) Light source: 150W xenon lamp 2) Wavelength range: Excitation (EX) 200nm~900nm; Emission (EM) 200nm~900nm. 3) Spectral bandwidth: Excitation 1nm, 2.5nm, 5nm, 10nm, 20nm; Emission 1nm, 2.5nm, 5nm, 10nm, 20nm. 4) Wavelength accuracy: Excitation wavelength ± 2nm, Emission wavelength ± 2nm.

  • Page 27: Software

    2.4 Software Smoothing; 1-4 Derivative; Retrieve peaks; Calculate spectrum peak area; Continuous scanning measurement; Synchronous scanning measurement; Sample Quantitation analysis; Draw the standard curve (1-3 order); Sample concentration; Spectra arithmetic; Save and open the spectra; Spectra window processing; Measure S/N ratio automatically; Measure D/S ratio automatically;...

  • Page 28: Chapter 3 Operation

    USB flash disk) 3.3 Uninstall FL Analyst Remove the FL970 Analyst software in the control panel of the PC. 3.4 Software Initialization and Exit Connect the instrument to the computer with a USB communication cable and turn on the power switch.
  • Page 29 Figure 3-1 Connection window of FL970 and the software After use, please exit the software and turn off the instrument according to the following steps: Click the “× ” in the upper right corner of the software interface, and the dialog box as shown in Figure 3-2 will appear;...

  • Page 30: Software Main Menu

    Click the “Connect” button and enter the main interface of FL analyst, as shown in Figure 3-3: Figure 3-3 Main menu of FL970 software Click the menu button of “Function” in the upper left corner, and there are 4 drop-down menus, which are respectively: ...
  • Page 31 Figure 3-4 Dialog box of openning the file 2） Overlay from a File Select the spectra you want to overlay. When selecting the spectra, note that we can only select the spectra that scan mode and data mode are the same. After overlaying the spectra, the interface is shown in Figure 3-5.
  • Page 32 Figure 3-5 Window of overlaying the spectra Figure 3-6 Color mark belts of the fluorescence intensity values of the two spectra When overlaying several spectra, we can select some of the spectra to be hidden or seen as needed, as shown in Figure 3-7. The data can be hidden by double-clicking the color mark belts above the spectra with the mouse.
  • Page 33 Figure 3-7 Window of displaying or hiding the spectra 3） Overlay Click “Overlay”, as shown in Figure 3-8. The overlay dialog box appears, as shown in Figure 3-9. Figure 3-8 Demo window of overlaying the spectra “Overlaid spectrum” is in the left of the dialog box, “Existing” is in the right of the dialog box.
  • Page 34 Figure 3-9 Dialog box of overlaying the spectra Data without an asterisk marker can be removed from “Existing” to “Overlaid Spectrum”. As shown in Figure 3-10 and Figure 3-11. Figure 3-10 Dialog box of removing spectra from “Existing” to “Overlaid Spectrum”...
  • Page 35 Figure 3-11 Demo dialog box of removing spectra from “Existing” to “Overlaid Spectrum” 4） Save As Save the spectrum as the corresponding type as needed. Instrument: Click “Instrument”, there are 5 submebus: “EM Wavelength Shift”, “EX Wavelength Shift”, “Concentration Measurement”, “Search Instrument” and “Initialize”. EM Wavelength Shift, EX Wavelength Shift EM, EX wavelength shortcut setting options.
  • Page 36 Settings: “Set <WL Scan> Parameters”, “Export <WL Scan> Parameters”, “Import Parameters”, “Import Standard Curve” (Effective when “Quantitation”) and “About FL Analyst” (FL970 software information, such as the version number) can be set in the submenu. As shown in Figure 3-14.
  • Page 37 Figure3-14 Demo window of Settings Close all data views: Click the icon as shown in Fig 3-15, all displayed spectra and data can be deleted. Figure3-15 Icon of closing all the data...

  • Page 38: Wavelength Scan

    3.6 Wavelength Scan At the end of initialization and the state of the instrument is “Ready”, click “WL Scan” in the left main function bar and enter the function interface of wavelength scan, as shown in Figure 3-16. Figure 3-16 Function interface of wavelength scan 3.6.1 Parameters Setting Click the “Setting”...
  • Page 39 As shown in Figure 3-18, the introduction of various system parameters of the instrument is as follows: Figure 3-18 Dialog box of Instrument parameters for WL Scan  Slit EX Slit and EM Slit of FL970 can be adjusted, which are respectively: 20nm, 10nm, 5nm,...
  • Page 40 2.5nm, 1nm and closed.  PMT Mode and PMT Value PMT Mode refers to the boosting mode of the photomultiplier. PMT Value can be entered in manual mode, the range is 0～900V. Open the cover plate of the sample compartment lid, the high voltage will automatically switch off.
  • Page 41  Data Interval Data interval of wavelength scan: 0.2, 1.0, 2.0, 3.0, 5.0, 10.0nm  Replicates Continuous scan of the times one through ten can be selected. Data Processing As shown in Figure 3-20, the parameters that are necessary for judging peak and valley are described as follows: ...

  • Page 42: Sample Measurement

    Figure 3-21 Dialog box of Plot parameters for WL Scan 3.6.2 Sample Measurement Click “Start” button in the right toolbar of “WL Scan”, and the status bar of the instrument prompts the conversion from “Ready” to “Scanning”, as shown in Figure 3-22. Note: the “Start”, “Stop”...

  • Page 43: Data Processing And Print

    The data window appears after the scan, as shown in Figure 3-23. There are several tool buttons in the toolbar above the window. The scan data area is on the right of the window, which can display three data types, such as Data list, values of Peak and Valley. The interval of the data list can be entered (in the right box of “Data”, and press “Enter”...
  • Page 44 Figure 3-24 Dialog box of spectra arithmetic for WL Scan △Note Arithmetic can only be performed between the two spectra with the same data pattern Calculate spectrum area Click “Calculate spectrum area” button, the window as shown in Figure 3-25 appears. Enter start wavelength and end wavelength, and click “Calc.
  • Page 45 Figure 3-25 Dialog box of calculating spectrum area for WL Scan View parameters Click “View parameters” button, and the window as shown in Figure 3-26 appears. There are four pages in the window of “Property of WL Scan”. For the first three pages, leave them as the default.
  • Page 46 Figure 3-26 Dialog box of clicking the “View parameters” button for WL Scan  The second page shows the system parameters of the instrument and the main parameters of wavelength scan, as shown in Figure 3-27. Figure 3-27 Dialog box of the system parameters of the instrument and the main parameters of WL Scan...
  • Page 47  The third page is the parameters of “Data Processing”, as shown in Figure 3-28. All parameters can be re-entered. Figure 3-28 Dialog box of Data Processing after clicking the “View parameters” button for WL Scan  The fourth page is “Print/Export”, as shown in Figure 3-29. Print report/export file, the checked items are displayed and the font and size can also be selected.
  • Page 48 Figure 3-29 Dialog box of Print/Export after clicking the “View parameters” button for WL Scan After setting the print options of scan property, click the print icon in the upper right of the spectrum window to print the related spectrum and data, etc. Click the “...
  • Page 49 Figure 3-30 Dialog box of “Save As” after clicking the excel icon for WL Scan Figure 3-31 Excel display window after clicking the “Save” button...

  • Page 50: Time Scan

    3.7 Time Scan At the end of initialization and the status of the instrument is “Ready”, click “Time Scan” in the left of the main function bar to enter the function interface of time scan, as shown in Figure 3-32. Figure 3-32 Function interface of Time Scan 3.7.1 Parameters Setting Click “Setting”...
  • Page 51 Figure 3-33 Dialog box of General parameters for Time Scan Instrument As shown in Figure 3-34, the introduction of various system parameters of the instrument is as follows: Figure 3-34 Dialog box of Instrument parameters for Time Scan...
  • Page 52  Slit EX Slit and EM Slit of FL970 can be adjusted, which are respectively: 20nm, 10nm, 5nm, 2.5nm, 1nm and closed.  PMT Mode and PMT Value PMT Mode refers to the boosting mode of the photomultiplier. PMT Value can be entered in manual mode, the range is 0～900V.
  • Page 53 The selection range is from 0 to 10. Data Processing： As shown in Figure 3-36, the parameters that are necessary for judging peak and valley are described as follows:  Threshold When the difference value between peak and valley is more than this value which the peak valley can be judged.

  • Page 54: Sample Measurement

    Figure 3-37 Dialog box of Plot parameters for Time Scan 3.7.2 Sample Measurement 1) Click “Start” button in the right toolbar of “Time Scan”, and the status bar of the instrument prompts the conversion from “Ready” to “Scanning”, as shown in Figure 3-48. Note: the “Start”, “Stop”...

  • Page 55: Data Processing And Print

    can be entered (in the right box of “Data”, and press “Enter” to confirm). The default value is the same as the scan interval. Figure 3-39 Display window of the end of time scan 3.7.3 Data Processing and Print As 3.6.3. 3.8 3D Scan initialization completion and the status of the instrument will show as “Ready”, click “3D Scan”...

  • Page 56: Parameters Setting

    3.8.1 Parameters Setting Click “Setting” button in the toolbar on the right of “3D Scan” to input the parameters. The window of “Setting” consists of 5 tabs.  General  Instrument  3D Scan  Data Processing  Plot 3D General As shown in Figure 3-41, the company name and the operator can be entered, and the instrument information can also be displayed.
  • Page 57 Figure 3-42 Dialog box of Instrument parameters for 3D Scan  Slit EX Slit and EM Slit of FL970 can be adjusted, which are respectively: 20nm, 10nm, 5nm, 2.5nm, 1nm and closed.  PMT Mode and PMT Value PMT Mode refers to the boosting mode of the photomultiplier. PMT Value can be entered in manual mode, the range is 0～900V.
  • Page 58 Figure 3-43 Dialog box of 3D scan parameters for 3D Scan  EM Start WL Start EM wavelength of 3D scan can be input from 200nm to 900nm with a minimum step size of 0.2nm, the start wavelength must be less than the end wavelength at least 1nm. ...
  • Page 59 Figure 3-44 Dialog box of Data Processing parameters for 3D Scan Plot 3D As shown in Figure 3-45, the parameters that are necessary for plotting are described as follows: Z Axis: The input range is -70000～70000. The recommended range is 0~70000. EX wavelength and EM wavelength show the maximum and minimum values that have been set currently.

  • Page 60: Execute

    3.8.2 Execute Click “Start”, at the end of the scan, the following 3D spectra appears (Figure 3-46): Figure 3-46 Display window of the end of 3D scan Interface Description： Figure 3-47 Icons of on the upper of the interface A——Reset B——Spectra arithmetic C——Wireframe/Contour Plane/Contour line/Surface (change the display mode) D——On/off color (display the color spectra or black-and-white specra)

  • Page 61: Stop

    Figure 3-48 Profiles of EM and EX of clicking any location of 3D spectra Figure 3-48 are the cross section diagrams of EX and EM, the EX cross section and EM cross section of the current click location can be selected when clicking the 3D spectra. 3.8.3 Stop Click “Stop”...

  • Page 62: Quantitation Measurement

    3.9 Quantitation Measurement At the end of initialization and the status of the instrument is “Ready”, click “Quantitation” in the left of the main function bar to enter the function interface of Quantitation measurement, as shown in Figure 3-49. Figure 3-49 Function interface of Quantitation 3.9.1 Parameters Setting Click “Setting”...
  • Page 63 As shown in Figure 3-51, the introduction of various system parameters of the instrument is as follows: Figure 3-51 Dialog box of Instrument parameters for Quantitation  Slit EX Slit and EM Slit of FL970 can be adjusted which are respectively: 20nm, 10nm, 5nm,...
  • Page 64 2.5nm, 1nm and closed.  PMT Mode and PMT Value PMT Mode refers to the boosting mode of the photomultiplier. PMT Value can be entered in manual mode, the range is 0～900V. Open the cover plate of the sample room, the high voltage will automatically switch off. Close the cover plate and it is necessary to reset in the interface of “Setting”.
  • Page 65 Standard(S) Figure 3-53 Dialog box of Standard(S) parameters for Quantitation The standard sample name, concentration, comment can be entered and sequence number will be numbered automatically. The information of the standard samples can be inserted, appended and deleted at the current location.

  • Page 66: Blank Measurement

    Figure 3-54 Dialog box of Sample(S) parameters for Quantitation The standard sample name, concentration and comment can be entered. The information of the samples can be inserted, appended and deleted at the current location. Plot Figure 3-55 Dialog box of Plot parameters for Quantitation As shown in Figure 3-55, the parameters that are necessary for plotting are described as follows: ...

  • Page 67: Standard Sample And Sample Measurement

    Figure 3-56 Dialog box after clicking the “Blank” button of Quantitation 3.9.2 Standard Sample and Sample Measurement After blank measurement, the sample measurement will be performed automatically, the Quantitation measurement of “Blank” logo will be red, as shown in Figure 3-57, standard sample measurement and sample measurement will automatically deduct the blank data after being reminded.
  • Page 68 Figure 3-58 Display window before blank measurement for Quantitation According to the parameters that have been set, click “Std. Meas.” to measure the standard samples successively. At the end of standard sample measurement, the data, calibration curve and fitted formula can be displayed, as shown in Figure 3-59. (Figure 3-59 is only for understanding the operation, no specific data value.) Figure 3-59 Display window of the end of “Std.

  • Page 69: Measurement Stop

    Figure 3-60 Display window of the end of “Smpl. Meas.” for Quantitation 3.9.4 Measurement stop Click “End” to end the measurement.

  • Page 70: Chapter 4 Maintenance

    7）Please make sure that the sampler chamber cover closed, as testing.  Fault handling In order to facilitate the maintenance, the user should explain the fault condition as accurately as possible when the maintenance personnel from Techcomp and its representative that will repair the instrument.
  • Page 71 Shanghai Techcomp Instrument LTD 16 building No.201 Mingyi RD. Songjiang District Shanghai +86-21-67687200 +86-21-67687190 sh-factory@techcomp.cn www.techcomp.cn...

Table of Contents