Macherey-Nagel NANOCOLOR 500 D Manual
  1. Manuals
  2. Brands
  3. Macherey-Nagel Manuals
  4. Analytical Instruments
  5. NANOCOLOR 500 D
  6. Manual

Macherey-Nagel NANOCOLOR 500 D Manual

Analyses of drinking, surface, ground, process and waste water
Hide thumbs
Table of Contents

Advertisement

Quick Links

Bedienungsanleitung
Manual
NANOCOLOR
Analysen von Trink-, Oberflächen-,
Grund-, Brauch- und Abwasser
Analyses of drinking, surface, ground,
process and waste water
www.mn-net.com
MACHEREY-NAGEL
MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Deutschland
Deutschland und weltweit:
Germany and worldwide:
Tel.:
+49 24 21 969-0
Fax:
+49 24 21 969-199
E-Mail: info@mn-net.com
Schweiz:
Switzerland:
Tel.:
+41 62 388 55 00
Fax:
+41 62 388 55 05
E-Mail: sales-ch@mn-net.com
®
500 D
Frankreich:
France:
Tel.:
+33 388 68 22 68
Fax:
+33 388 51 76 88
e-mail: sales-fr@mn-net.com
MN
MACHEREY-NAGEL
MACHEREY-NAGEL
ZERTIFIZIERT
EN ISO 9001: 2008
EN ISO 9001: 2008
CERTIFIED

Advertisement

Table of Contents
loading

Summary of Contents for Macherey-Nagel NANOCOLOR 500 D

  • Page 1 Analysen von Trink-, Oberflächen-, Grund-, Brauch- und Abwasser Analyses of drinking, surface, ground, process and waste water www.mn-net.com MACHEREY-NAGEL MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Deutschland Deutschland und weltweit: Schweiz: Frankreich: MACHEREY-NAGEL MACHEREY-NAGEL Germany and worldwide:...
  • Page 2 CONTENTS Page General part (white pages) Photometer NANOCOLOR ® 500 D Technical data Design of the photometer Filters Power supply and light source Interface description Barcode Update via INTERNET Preparation for operation Calibration of the NANOCOLOR ® 500 D Operation Keyboard 3.1.1 Special functions of certain keys...

  • Page 3: Technical Data

    – 8 9/336/EEC of 05.03.1989 (including Amendment Directive 92/31/EEC) – EMV Directive Disposal in accordance with EU directive 2002/96/EC In compliance with EU directive 2002/96/EC, MACHEREY-NAGEL will accept back and dispose of the old instrument at no cost. ATTENTION! It is not allowed to dispose of the instrument using mu- nicipal waste disposal services.
  • Page 4 1.2 Design of the photometer 05.07.04 09:44 0042 AMMONIUM 10 3,6 mg/L 0001 Ort 1 graphic display 128 x 64 pixels 2 20-part keyboard 3 barcode scanner 4 cuvette slot with automatic cuvette identification 5 socket for connection of mains adaptor 6 RESET push button (subsurface) 7 USB (1.1) interface for data transfer and update 8 RS 232 C interface for suitable printers and data transfer 500 D 01/2012...
  • Page 5 1.3 Filters When the photometer NANOCOLOR ® 500 D is switched on, it automatically per- forms a filter test. If a deviation is found, the program asks for a new calibration. Filters which have left the confidence interval, are displayed as <check filter>. In order to prevent wrong measurements, further evaluation of tests which require the defective filter is blocked. Filter Wavelength of Color of the Color impression position the light [nm] the filter [nm] light / filter...
  • Page 6 WARNING: the barcode scanner is a Laser class 1 product. Do not look straight into the scanner during reading. 1.7 Update via INTERNET For updating the internal program of the NANOCOLOR ® 500 D, the latest version can be downloaded from the MACHEREY-NAGEL homepage under www.mn-net.com. Follow the instructions given there. 500 D 01/2012...

  • Page 7: Preparation For Operation

    16:06 17.11.11 16:06 CALIBRATION CALIBRATION please wait insert cal.-tube 4. After calibration again the method selection mode is activated. 17.11.11 16:06 _ _ _ Method: >> >> cuvette 5. The calibration can be activated at any time from the configuration menu. Filters which have left the confidence interval are displayed as <check filter> (repeti- tion of the calibration, filter cleansing or filter change necessary). In order to prevent wrong measurements, further evaluation of tests which require the defective filter is blocked. If all filters are displayed as <error>, most probably the lamp is defective. Please contact MACHEREY-NAGEL. 500 D 01/2012...
  • Page 8 Operation 3.1 Keyboard The photometer NANOCOLOR ® 500 D has a 20-part keyboard: 10 numbers and 10 keys with special functions. The whole field is covered with plastic foil and therefore protected against liquid spills. Tactile feedback for all keys increases safety of operation. Explanation of individual keys On/Off switch of the photometer Keys For entering digits, letters or special characters...
  • Page 9 3.1.1 Special functions of certain keys When the photometer has just been switched on, pressing key calls up the last-used method. Pressing key in method selection mode directly calls up the extinction pro- gram. Pressing key in method selection mode calls up memory administration. MEM.
  • Page 10 User guidance The NANOCOLOR ® 500 D now governs the further course of the measurement by optical display instructions: measuring range and dimension, reaction time, zero adjustment, sample num- ber, sampling location, dilution and readiness to measure any number of samples If the measuring range is exceeded, or if any other errors arise, these will be indicated on the display immediately.
  • Page 11 3.3 Scheme of operation for NANOCOLOR ® tests 3.3.1 Scheme of operation when using tube tests with barcode desired parameter: e.g. COD 160 test 0-26 1. Key (if other parameters have been measured before) 17.11.11 16:06 _ _ _ Method: >> >> cuvette 2. Insert coded test tube into the photometer. Display of measuring range 17.11.11 16:06...
  • Page 12 3. Press key first measuring range is displayed with dimension; press key , until required measuring range and dimension appear (can be repeated endlessly in roll mode or back with key 17.11.11 16:06 17.11.11 16:06 0041 AMMONIUM 10 0042 AMMONIUM 10 0.2-8.0mg/L NH4N 0.2-10.0mg/L NH4 This can also be attained if the fourth digit of the 4-digit test number, e.g.
  • Page 13 8. For entering a sampling place press key . The name of a sampling location may have up to 12 alphanumeric characters. Up to 20 places are stored in a list and can be selected or edited using keys 17.11.11 16:06 17.11.11 16:06...
  • Page 14 4. C onfirm required measuring range and dimension by pressing key (does not apply when 4 digits are entered). The interference filter is automatically turned into measuring position. 5. Reaction time is displayed, start with key or skip with key 17.11.11 16:06 1671 NITRITE 10’00 React.time 6. Place cuvette with blank value in photometer and press for zero adjustment NULL ZERO (use same cuvette size as for sample, e.g. 10 mm).
  • Page 15 10. For entering a dilution press key drücken. The dilution is entered as 1 + x, i. e. a dilution of 1 : 25 is entered as 1 + 24. After input the dilution is directly used to calculate and display the correct result. 17.11.11 16:06 17.11.11...
  • Page 16 Measurement with factor 1. Select method 901 2. The preset wavelength is displayed. Select the required wavelength using keys and confirm the selection by pressing . The selected wavelength is automatically adjusted. 3. Enter factor via keyboard. The accuracy of the display is determined by the num- ber of decimal places of the factor.
  • Page 17 3.4.3 903 EXTINCTION The extinction is a basic term in photometry, upon which all other definitions are based. light transmitted through blank value extinction = logarithm ( light transmitted through test sample The practical NANOCOLOR ® analytical system gives direct reading of results. In spite of this fact, and especially in cases where the test results are near the limits of the range, extinction should not be ignored, since the four digit display does not offer any information about accuracy and consistency of test results.
  • Page 18 3.4.5 905 TRANSMISSION In addition to the absorbance, transmission is a very significant variable in photo- metry. Transmission is the transparency of a sample solution to the light used for measurement. The transmission is expressed directly as percentage. light transmited through test sample Transmission in % = x 100 light transmited through blank value light transmited through test sample The dimensionless variable is referred as the...

  • Page 19: Special Functions

    3.5 Special functions 3.5.1 Display of extinction After measurement 17.11.11 16:06 0042 AMMONIUM 10 8,0 mg/L 0001 place the extinction of the measured value can be displayed by pressing 17.11.11 16:06 0042 AMMONIUM 10 1.090 E 0001 place Display of extinction, when the measuring range is exceeded After measurement 16.11.11 16:06...
  • Page 20 3.5.3 Memory administration In the standard mode of operation storage is activated (indicated by a ✔ between time and battery display. 17.11.11 16:06 ✓ 0042 AMMONIUM 10 8,0 mg/L 0001 place As long as the cuvette is still in the cuvette slot, pressing key when storage is MEM.
  • Page 21 Printing and output When you press key at this point, all stored data are transferred via the 9-pin serial interface to a ready serial printer to be printed. (Make sure that the transfer rates of printer and photometer are set to the same values, e.g.
  • Page 22 Enter the required method. 17.11.11 16:06 ✓ MEMORY: Method: When the method is entered with four digits, all data belonging to the specific submethod are selected. When you enter 3 digits of the method number, all sub- methods of this test (e.g. 0041–0049) are selected. Input of a method number can be skipped by pressing In this case all tests are selected, or you skip selection by method using key access the next selection step sampling location.
  • Page 23 3.5.4 Special (user-defined) methods The NANOCOLOR ® 500 D offers the possibility to program up to 100 user-specific methods. These methods can be defined as linear (factor) or as non-linear methods (up to 4th degree functions). For defined methods the same options are available as for the pre-programmed methods (i. e. sampling location, dilution, storage etc.). Selection of a special method Special methods are activated from the main menu by pressing 17.11.11 16:06 P __ Method: special method New method Call up new method – for example P56 – by pressing key and 56.
  • Page 24 Running a special method Entering the 2-digit number, e.g. P56, activates a previously defined special method. If you do not remember the number of a special method, you can use keys then to scroll through all user-defined methods. 17.11.11 16:06 17.11.11 16:06 P __ Method: Selenium run ? special method...
  • Page 25 3.5.5 Numbering of samples For an unambiguous identification of samples, every measurement is automatically assigned a sample number (starting with 0001). Further measurement values are numbered in sequence. It is possible to assign another sample number to each value directly after measurement. Before you remove the cuvette from the cuvette slot, press key and enter the 4-digit sample number.
  • Page 26 3.5.7 Dilution of samples In analytical practice it is often necessary to dilute a sample, until the concentration to be determined is within the measuring range. Example: expected measurement value: between 80 and 200 mg/L range of photometric test: 0.1–10.0 mg/L required dilution (200 → 10 mg/L): at least 1 : 20 recommended dilution:...
  • Page 27 3.6 Configuration of the NANOCOLOR 500 D ® In method selection mode enter ) * * . All topics of the configuration can be selected in the scroll mode using keys 1. Select language and confirm with 17.11.11 16:06 17.11.11 16:06 CONFIGURATION CONFIGURATION (GB) ---> (?) language: (GB) ? → date / time calibrate memory Wait until your language is displayed, then press key...
  • Page 28 4. Select memory and confirm with 17.11.11 16:06 17.11.11 16:06 17.11.11 16:06 ✓ _ _ _ Method: CONFIGURATION CONFIGURATION memory (GB) ---> (?) → >> >> date / time cuvette calibrate memory → Using keys turn memory on or off and confirm with key 5. Select reaction time and confirm with 17.11.11 16:06 17.11.11 16:06 CONFIGURATION CONFIGURATION react.time date / time →...
  • Page 29 8. Select heading and confirm with 17.11.11 16:06 17.11.11 16:06 CONFIGURATION CONFIGURATION NANOCOLOR 5OOD_ react.time COM 1 signal tone → heading Using keys you can edit the heading or you may enter a new heading using the keyboard of the photometer. 9. Select special filters 11 and 12 and confirm with 17.11.11 16:06 17.11.11 16:06...
  • Page 30 USB interface. Start the update pro- gramme on the PC and on the photometer. After a successful update the pho- tometer returns to the method selection mode. MACHEREY-NAGEL MACHEREY-NAGEL UPDATE BIOS SP-A5 V1.1...
  • Page 31 3.7 Data transfer The NANOCOLOR ® 500 D can communicate with almost any conventional terminal software. For this purpose it features choice of two different interfaces: – Serial RS 232 standard interface – USB port 1.1 For data transfer you need either a serial zero modem cable (9/9 pins, REF 919 680) or a standard USB cable (plug A / plug A).

  • Page 32: Troubleshooting

    3.8 Troubleshooting Message Explanation Suggested remedy recharge battery battery voltage is below the recharge battery with the charger minimum for about 14 h; photometer can be operated during charging memory the memory of the photome- accept message by pressing full ter contains the maximum of and continue measurements without 500 data sets...
  • Page 33 Preparation of samples Water samples are not always suitable for immediate analyses. In the case of heav- ily polluted water (or waste water) especially, it is often not possible to conduct analyses without pretreating the sample first; otherwise, larger concentrations of organic or inorganic compounds can interfere and lead to falsely negative or posi- tive test results.
  • Page 34 4.1 Crack Set Method: Dissolution and decomplexation of heavy metals with sulfuric acid and potassium peroxodisulfate NANOCOLOR ® reagent set: Crack set (REF 918 08) Requisite NANOCOLOR accessories: ® heating block, decomposition apparatus (REF 916 29), piston pipette with tips Precautions: Beware of samples containing high concentrations of cyanides – poisonous vapours will develop! Procedure: Switch on heating block, set to 100 °C and 1 h 00 min.
  • Page 35 4.2 NANOCOLOR ® NanOx Metal for oxidative decompositon of metals and total phosphorus NanOx Metal: NanOx decomposition reagent and NanOx neutralization reagent Principle: Oxidative decomposition for determination of complexly bonded metals or metal ions, which are present in an oxidation state, which without decomposition would not be determined by the test (e.g. chromium(III)). Also suitable for the deter- mination of total phosphorus by oxidation of all inorganic and organic phosphorus compounds to form ortho-phosphate.
  • Page 36 Procedure Pipet 10 mL sample solution into the decomposition vessel, add 2 level orange measuring spoons NanOx Metal decomposition reagent, close and shake thor- microwave: oughly. Place pressure vessel on the outer rim of the turntable in the microwave oven and heat 23 s at 900 VA or 28 s at 750 VA (always use the highest setting of the respective instrument). Remove vessel from the microwave oven and let cool for about 10 min.
  • Page 37 4.3 NANOCOLOR ® NanOx N for oxidative decomposition of total nitrogen NanOx N: NanOx decomposition reagent and NanOx compensation reagent Principle: Oxidation of all inorganic and organic nitrogen-containing substances to form nitrate. Residues of peroxide which remain after oxidation and oxidised chromium(VI), which interfere with the determination of nitrate, are eliminated by a compensation reagent.
  • Page 38 5.11 Procedures for photometric analyses with NANOCOLOR ® tests when samples are colored or turbid These procedures can only be used in connection with the corresponding original instructions in this NANOCOLOR ® manual. The photometric analysis of water samples with own color or turbidity always requires determination of a correction value.
  • Page 39 5.11.1 Determination of correction values for NANOCOLOR ® tube tests For measurement of the correction value use as a blank value a clean, empty test tube filled with distilled water (exceptions: test 0-64/0-65/0-66). Test Test tube for correction (value B) 0-03, 0-04, 0-05, 0-06, Proceed as described in the instructions for test 0-03 / 0-04 / 0-05 / 0-06 / 0-08, but do not add NANOFIX R2, close, mix.
  • Page 40 Test Test tube for correction (value B) 0-58 Manganese 10 Fill empty test tube with 4.0 mL sample solution, 0.5 mL distilled water and 0.5 mL R2, close and mix. Add 1 measuring spoon R3, close and shake vigorously. 0-61 Nickel 7 Proceed as described in the instructions for test 0-61, but instead of 1.0 mL R2 add 1.0 mL NaOH 14 %.
  • Page 41 5.11.2 Determination of correction values for NANOCOLOR ® standard tests with reagent blank value For tests of this category, the instructions require distilled water plus reagents as a reagent blank value. Another volumetric flask 25 mL is necessary for the correction value. Distilled water is used as a blank value for the correction value. For some tests, a special reagent has to be prepared for correction.
  • Page 42 5.11.3 Determination of correction values for NANOCOLOR ® standard tests with sample blank value For the tests of this category, the correction can already be performed during the measurement. In these cases the following preparations are used as blank values instead of the blank values given in the instructions.

Table of Contents