SCIEX ExionLC Operator's Manual
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SCIEX ExionLC Operator's Manual

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ExionLC
PDA Detector
Operator Guide
RUO-IDV-05-1842-B
October 2015

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Summary of Contents for SCIEX ExionLC

  • Page 1 ExionLC PDA Detector Operator Guide RUO-IDV-05-1842-B October 2015...
  • Page 2 SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX s sole and exclusive representations, warranties, and obligations.

  • Page 3: Table Of Contents

    Replace the D2 Lamp...............................28 Replace the Dust Filter..............................31 Clean the Module Surfaces..............................33 Chapter 6 Troubleshooting........................34 Corrective Action for Bubbles............................39 Insulation Tube................................39 Corrective Action against Leaks from Cell Window......................40 Appendix A Error Messages........................41 Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 3 of 47...
  • Page 4 Contents Appendix B Status Panel..........................44 Appendix C Consumables and Spares.....................46 Revision History............................47 ExionLC PDA Detector Operator Guide 4 of 47 RUO-IDV-05-1842-B...

  • Page 5: Chapter 1 Introduction

    Introduction This guide describes the basic operation and troubleshooting for the ExionLC PDA Detector . Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this guide. This guide provides safety instructions and precautions to make sure that the user operates the system safely.

  • Page 6: Protective Earth Conductor

    Follow local municapal waste ordinances for proper disposal provisions to reduce the environmental impact of WEEE (waste, electrical and electronic equipment). To safely dispose of this equipment, contact a local Customer Service office for complimentary equipment pickup and recycling. ExionLC PDA Detector Operator Guide...

  • Page 7: Ventilation Precautions

    Introduction Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE to obtain a copy of the form. Ventilation Precautions The venting of fumes and disposal of waste must comply with all of the federal, state, provincial, and local health and safety regulations.

  • Page 8: Equipment Use And Modification

    WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts not recommended by SCIEX or use of parts for any use other than their intended purpose can place the user at risk of harm or negatively impact system performance.

  • Page 9: Chapter 2 Hazard Symbols

    This section describes some occupational health and safety symbols found in the documentation and laboratory environment. Table 2-1 General Hazard Symbols Safety Symbol Description Personal Injury Hazard Table 2-2 Chemical Hazard Symbols Safety Symbol Definition Biohazard Explosion Hazard Toxic Chemical Hazard Fire Hazard Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 9 of 47...

  • Page 10: Documentation Symbols And Conventions

    CAUTION: Caution signifies an operation that could cause damage to the system or corruption or loss of data if precautions are not followed. Note: Note emphasizes significant information in a procedure or description. ExionLC PDA Detector Operator Guide 10 of 47...
  • Page 11 Tip! Tip provides useful information that helps apply the techniques and procedures in the text for a specific need and provides shortcuts, but is not essential to the completion of a procedure. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 11 of 47...

  • Page 12: Chapter 3 Overview

    Overview The ExionLC PDA Detector is a photodiode array (PDA), UV-Vis detector designed for use with an ExionLC ultra high performance liquid chromatograph system (HPLC), and was developed to satisfy demands for greater accuracy and sensitivity in analysis. A photodiode array detector continuously monitors spectra over the entire wavelength range. Use of a deuterium (D2) lamp as the light source allows high sensitivity measurement of chromatograms and absorbance spectra over a wavelength range of 190 nm to 700 nm.
  • Page 13 Front cover. Open this cover to install or perform maintenance on the flow cell. Power switch. Turns the power on and off. Left-side cover. Open this cover to replace the lamp. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 13 of 47...
  • Page 14 Flow cell connector Connects to the flow cell. Tubing holder Secures the tubing. Coupling holder The retaining plate and screw secure the coupling in place. Flow cell Installation is performed using the coupling. ExionLC PDA Detector Operator Guide 14 of 47 RUO-IDV-05-1842-B...
  • Page 15 Connects to the provided drain tubing. If liquid leaks inside the module, it is drained out through this opening. Shipping screw Protects the internal measurement components from vibration during transport. It is removed after installation. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 15 of 47...
  • Page 16 Fan for internal cooling (keep a minimum of 100 mm between the rear of the module and the wall.) INITIALIZE button Used to initialize the module. CLEAR button Press this button to clear errors. ExionLC PDA Detector Operator Guide 16 of 47 RUO-IDV-05-1842-B...

  • Page 17: Chapter 4 Operating Instructions

    • Flow cell bubble removal and liquid leakage check Refer to Corrective Action against Leaks from Cell Window. • Lamp light intensity check • Wavelength accuracy check Refer to Service and Maintenance Information. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 17 of 47...

  • Page 18: Precautions For Wavelength Calibration And Wavelength Accuracy Check

    In addition, if the air conditioner is turned off, or if for some other reason the room temperature changes too much, the temperature control on the optical system might become inadequate. Turn On the Module • Press the power switch. ExionLC PDA Detector Operator Guide 18 of 47...
  • Page 19 Note: Whether the D2 lamp is turned on or not is determined by the setting at the time of the last module shutdown. If an error occurs, the status indicator turns red. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 19 of 47...

  • Page 20: Chapter 5 Service And Maintenance Information

    Maintenance, Inspections, and Adjustment WARNING! Personal Injury Hazard. Contact the SCIEX representative if product installation, adjustment, or relocation is required. WARNING! Electrical Shock Hazard. Always turn off the power and then unplug the instrument prior to performing inspection and maintenance.

  • Page 21: Periodic Inspection And Maintenance List

    Refer to Clean or Replace the Flow Cell. D2 lamp replacement Replace at about 2000 hours of total illumination time. Refer to Replace the D2 Lamp. Figure 5-1 PDA Detector Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 21 of 47...

  • Page 22: Clean Or Replace The Flow Cell

    3. Loosen the coupling mounting screw and then remove the coupling. 4. Disconnect the connector. 5. Loosen the two cell mounting screws and then remove the cell. Figure 5-2 Flow Cell ExionLC PDA Detector Operator Guide 22 of 47 RUO-IDV-05-1842-B...

  • Page 23: Clean The Flow Cell

    Note: If an aqueous solvent (especially a solvent containing buffer salts) is used as the mobile phase, purge the solvent in the cell with water before flushing with isopropanol. If an organic solvent is mixed with a buffer solution, crystals will form and clog the tubing. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 23 of 47...
  • Page 24 Service and Maintenance Information Figure 5-3 Required Materials Item Description Cell inlet tubing Waste container Cell outlet tubing Male nut EFTE Coupling 1.6-0.8C Male nut 0.8MN-PEEK Syringe needle ExionLC PDA Detector Operator Guide 24 of 47 RUO-IDV-05-1842-B...
  • Page 25 3. Connect the syringe to the cell outlet tubing. (Attach the male nut 0.8MN-PEEK to the syringe needle and then connect the coupling 1.6-0.8C.) WARNING! Puncture Hazard. Handle the needle with care. The tip of the needle is extremely sharp. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 25 of 47...
  • Page 26 Item Description Male nut EFTE Coupling 1.6-0.8C Male nut 0.8MN-PEEK Syringe needle Syringe 4. Gently press the syringe plunger. The isopropanol is injected into the cell interior and cleans the cell. ExionLC PDA Detector Operator Guide 26 of 47 RUO-IDV-05-1842-B...

  • Page 27: Install The Flow Cell

    5. Connect the cell inlet tubing to the column and the cell outlet tubing to the waste container. Note: When performing the above operation, do not let any air enter the flow lines. 6. Reattach the front cover. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 27 of 47...

  • Page 28: Replace The D2 Lamp

    1. Turn off the module and unplug it from the wall. 2. Open the front cover and left side cover. 3. Loosen the lamp cover mounting screw and then remove the lamp cover. ExionLC PDA Detector Operator Guide 28 of 47...
  • Page 29 5. Loosen the two D2 lamp mounting screws and then pull the lamp out of the module. Release the stopper by pinching the part indicated by arrows in the Figure 5-8 on page 30, and then pull out the connector. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 29 of 47...
  • Page 30 7. Install the lamp cover in its original position on the module. Install the lamp cover by inserting its edge into the slit in the panel and then secure it by tightening the lamp cover mounting screw. ExionLC PDA Detector Operator Guide...

  • Page 31: Replace The Dust Filter

    A dust filter is installed on the right side of the module. If the dust filter becomes clogged, the performance of the module deteriorates and even cause module failure. If the dust filter becomes discolored from its original white, replace it with a new one. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 31 of 47...
  • Page 32 Filter cover 2. Replace the filter and then return the filter cover to its original position. Align the catch on the filter cover with the chassis of the module when fitting the cover. ExionLC PDA Detector Operator Guide 32 of 47...

  • Page 33: Clean The Module Surfaces

    CAUTION: Potential System Damage. Do not allow spilled water to remain on the instrument surface and do not use alcohol or thinner-type solvents to clean the surfaces. Doing so can cause rusting and discoloration. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 33 of 47...

  • Page 34: Chapter 6 Troubleshooting

    Troubleshooting If a problem cannot be resolved even after taking the corrective action specified in the table, or if a problem is not included in the following tables, contact your SCIEX representative. Table 6-1 Electrical System Issues Symptom Probable Cause...
  • Page 35 • Put the tip of the detector drain tube in liquid and determine whether bubbles are exiting the tube. • If bubbles are flowing through the cell, perform corrective action for bubbles. Refer to Corrective Action for Bubbles. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 35 of 47...
  • Page 36 If the degree of drift decreases, the mobile phase might have impurities or the column might be insufficiently equilibrated. Inspect the mobile phase, column, and analysis parameters. ExionLC PDA Detector Operator Guide 36 of 47 RUO-IDV-05-1842-B...
  • Page 37 • In case of liquid leaks from the flow cell window, soak up the leak and then tighten the cell window fixing screw. • Refer to Corrective Action against Leaks from Cell Window. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 37 of 47...
  • Page 38 The cell is dirty. Clean the flow cell. Refer to Clean the Flow Cell. The mobile phase absorbs strongly. Inspect the mobile phase solvent and flow lines, and remove any impurities. ExionLC PDA Detector Operator Guide 38 of 47 RUO-IDV-05-1842-B...

  • Page 39: Corrective Action For Bubbles

    In such a case, attach the provided insulation tube to the inlet plumbing. This will insulate the plumbing between the oven and detector, reducing the baseline fluctuation due to ambient temperature change. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 39 of 47...

  • Page 40: Corrective Action Against Leaks From Cell Window

    (groove width 1 mm, groove length 8 mm) clockwise with a flat-bladed screwdriver. 2. If the leak does not stop even after tightening the screw, contact your SCIEX representative. Figure 6-1 Corrective Action Against Leaks from Cell Window...

  • Page 41: Appendix A Error Messages

    D2 lamp is over current. The D2 has lamp malfunctioned. Replace the D2 lamp. Refer to Replace the D2 Lamp. The lighting circuit has Turn off the power and then malfunctioned. contact the FSE. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 41 of 47...
  • Page 42 0x8945 Photo diode is saturated. The signal is saturated because Perform exposure time the exposure time for the PDA optimization using SPDM30A elements is inappropriate. Utility. ExionLC PDA Detector Operator Guide 42 of 47 RUO-IDV-05-1842-B...
  • Page 43 The D2 has lamp malfunctioned. Replace the D2 lamp. Refer to Replace the D2 Lamp. A malfunction has occurred inside Turn off the power and then the module. contact the FSE. Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 43 of 47...
  • Page 44 Status Panel Note: The status panel screen might become hot when in use. Figure B-1 Status Panel: Names and Functions ExionLC PDA Detector Operator Guide 44 of 47 RUO-IDV-05-1842-B...
  • Page 45 CONNECT. Illuminates when the module is connected to the system controller and the computer. Status indicator: • Green: Power is on • Red: Error • Orange: In sleep mode Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 45 of 47...
  • Page 46 Consumables and Spares Option Part No. Features D2 (deuterium) lamp 228-54515 Dust filter 228-54534 Male nuts ETFE S228-5675 Insulation tube S228-56760 Flat-bladed screwdriver S086-10504-18 ExionLC PDA Detector Operator Guide 46 of 47 RUO-IDV-05-1842-B...
  • Page 47 Revision History Revision Description Date First release of document. April 2015 Remove incorrect graphics from Troubleshooting section. October 2015 Operator Guide ExionLC PDA Detector RUO-IDV-05-1842-B 47 of 47...

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