Summary of Contents for Santai Technologies SepaBean
- Page 1 SepaBean machine Hardware Manual Santai Technologies, Inc. Release Date: May 2017...
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Page 2: Table Of Contents
1.1.Product Description 1.2.Product Features 1.3.Technical Parameters 1.4.Working Principle 02 Installation 03 Networking 04 Debugging 05 Operation 5.1.Method Setup 5.2.Run Setup 06 Instrument Maintenance 6.1.Change of Mobile Phase 6.2.Fuse Replacement 6.3.Hardware Maintenance 6.4.General Instrument Issues and Troubleshooting Hardware Manual SepaBean machine... -
Page 3: Preface
Preface Thank you very much for purchasing the SepaBean machine, the flash chromatography system by Santai Technologies, Inc (hereinafter called Santai for short). In this manual, the operation and maintenance of the SepaBean machine is described in detail. For the proper use of the system and maximizing the output, user is recommended to carefully read the instructions and safety measures as good laboratory practice. -
Page 4: Notices
Changes or modifications not solely approved by Santai could void the user's authority to operate the equipment. The use of replacement, consumable components, and accessories from other suppliers instead of Santai is not recommended. Use such parts in SepaBean machine will be a sole responsibility of user or related company. - Page 5 Always use the provided power chord with the system. If it is needed to use any other standard power cord, please check if the power cord meets the instrument voltage specifications and requirements. Hardware Manual SepaBean machine 3 of 27...
- Page 6 Warning High Temperature Surface. This symbol indicates that the surface is too hot to touch with hands. It may cause burns if not handled properly. Warning Unknown Risks. User must be vigilant when using the system. Hardware Manual SepaBean machine 4 of 27...
- Page 7 Standard Operating Procedure (SOP). SepaBean machines should be installed and stored in a GLP environment and operated in the best suitable conditions. Any instrument issue or safety issue must be informed to the dealer or Santai directly as soon as possible.
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Page 8: Overview
The SepaBean machine comprises of machine body, iPad, SepaBean software and components related to the wireless communication. The instrument has various modules integrated namely HPLC pump module, UV detector module, fraction collector module, control module, alarm module, cartridge arm and test module with some minor components. - Page 9 Function A- Wipe Function B- Wake System Components Description 1.iPad and iPad holder – iPad is used to control the SepaBean machine and it is installed on the holder which can be adjusted to operate the iPad at eye level.
- Page 10 11.Solvent Bottle Trays – Hold various sizes of solvent bottles. 12.Emergency stop Stop Button – When pressed, it will stops the instrument in emergency conditions. Hardware Manual SepaBean machine 8 of 27...
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Page 11: Product Features
This enables user to achieve the optimization of for the separation and purification methods. By accessing CBG global knowledge database, SepaBean machine will become more efficient in the development for purification method and data processing. - Page 12 1.2.3. Data sharing – User can access the data from multiple SepaBean machines within a network group. This enables user to get help for critical compounds or difficult separations methods from other groups. Also the same machine can access the CBG global knowledge database for better understanding.
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Page 13: Technical Parameters
Provide flexible installation mode and Auxiliary column holder use of the column in series SepaBean machine OS has APP software, with TLC to gradient optimization, automatic determination of gradient method, flash column size and sample Software loading determination according to the sample’s Rf value. Control system is... -
Page 14: Working Principle
1.4.Working Principle 1.4.1. SepaBean machine modules 1.4.1.1. Pump module – The pump module consists of two valveless binary metering pumps with high accuracy. This module works by drawing the solvent from bottles, mixing the solvent according to the gradient and isocratic elution profile entered by user and passing it through the column for sample separation. - Page 15 With user’s permission, the data from the local database can be uploaded on the CBG global knowledge database. Please note that the local data on SepaBean machine is 100% secure and confidential. The data cannot be shared without multiple and multilevel permissions.
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Page 17: Installation
2.Installation 2.1.Unpacking 2.1.1. The SepaBean machine and its accessories are packed in one box. When box is opened, please check if any signs of damage during shipment to the system or accessories. If any part is broken, please contact Santai or the dealer. - Page 18 2.2.Tubing Installation Please connect all the solvent and waste tubings as per diagram sticker at the back of the instrument. Figure 3. Solvent Waste Connections Map Hardware Manual SepaBean machine 16 of 27...
- Page 19 2.Use GB general power cord 3.The Personal Protective Equipment (PPE) must be always worn viz. gloves, glasses, aprons and shoes 4.Remove the spilled solvent or material immediatel 5.Ensure the safety of solvent bottles 6.Obey the No-Smoking rules Hardware Manual SepaBean machine 17 of 27...
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Page 20: Networking
Wireless Connection – If you don’t connect a network cable, SepaBean machine connects automatically with the Wi-Fi router. The default factory set user’s name and password should be transferred to the router. -
Page 21: Debugging
4.Debugging 4.1.Boot – Plug in the power cord to SepaBean machine. The instrument boots with various selftest going on the background. The main screen on the system displays the results of the self- tests viz. pumping system selftest, detector system self- test, fraction collector selftest, column arm test and other internal processes. -
Page 22: Operation
Ideally, 3-5 column volumes (CV) should be passed through the cartridge to achieve enough equilibration. User can modify it anytime. Hardware Manual SepaBean machine 20 of 27... - Page 23 C18 series. A mixture of Methanol/ACN and water in 80:20 ratio should be pumped through the column for considerable duration time. The plugs should be put on the inlet and outlet before storing it in a cool place. Hardware Manual SepaBean machine 21 of 27...
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Page 24: Instrument Maintenance
Remove the power cord from the power entry module. With the help of flathead screwdriver, open the fuse compartment. Replace the old fuse with the new one of the correct rating and push the fuse box back in place. Hardware Manual SepaBean machine 22 of 27... -
Page 25: Hardware Maintenance
Tighten the two screws to fix both the lamps. Push the tray back, connect the lamp cables, fix the hand-tightened screw and put the side panel back in its place with two Allen screws. Hardware Manual SepaBean machine 23 of 27... - Page 26 Quarterly or as and when the lamp energy falls below the limit or in case of UV lamp increasing noise Quarterly or whenever UV sensitivity is Cleaning flow cell reduced decreasing Cleaning flow cell Daily Hardware Manual SepaBean machine 24 of 27...
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Page 27: General Instrument Issues And Troubleshooting
Clean the solvent bottles or Dirty solvent bottles replace them with new ones Clean the column and system with Contaminants from a strong solvent to remove any the previous run contaminant Hardware Manual SepaBean machine 25 of 27... - Page 28 Check and replace the pressure sensor sensor Solvent pressure Refer to“High Backpressure” exceeds max section Pumps stop pressure limit during the run Check the external ventilation and Pumps overheated instrument fan at the back Hardware Manual SepaBean machine 26 of 27...
- Page 29 Fraction Fraction collector collection not Contact Santai Service needs calibration centered Arm is getting Fraction Check and remove any obstruction obstructed Collector arm doesn’t move Check and replace if necessary Motor is faulty Hardware Manual SepaBean machine 27 of 27...