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OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION OF THE PDES-01DM PNEUMATIC DRUG EJECTION MODULE WITH ANALOG TIMER FOR EPMS-07 SYSTEMS VERSION 1.3 npi 2016 npi electronic GmbH, Bauhofring 16, D-71732 Tamm, Germany Phone +49 (0)7141-0730230; Fax: +49 (0)7141-9730240 support@npielectronic.com; http://www.npielectronic.com...
(e.g. for diagnosis or treatment of humans) or for any other life-supporting system. npi electronic disclaims any warranties for such purpose. Equipment supplied by npi electronic must be operated only by selected, trained and adequately instructed personnel.
2. EPMS-07 Modular Plug-In System 2.1. General System Description / Operation The npi EPMS-07 is a modular system for processing of bioelectrical signals in electrophysiology. The system is housed in a 19” rackmount cabinet (3U) has room for up to 7 plug-in units.
Note: The chassis of the PWR-03D is connected to protective earth, and it provides protective earth to the EPMS-E housing if connected. Figure 3: Left: PWR-03D front panel view Right: PWR-03D rear panel view. Note: This power supply is intended to be used with npi EPMS-E systems only. version 1.3 page 5...
PDES-01DM User Manual 2.7. System Grounding EPMS-07/EPMS-03 The 19" cabinet is grounded by the power cable through the ground pin of the mains connector (= protective earth). In order to avoid ground loops the internal ground is isolated from the protective earth.
= 4 bar (58 psi) or 2 bar (29 psi). The device is designed as a module for the npi EPMS-07 system. It comprises of one channel consisting of a precision pressure regulator, an electronically controlled valve, (or a very fast micro valve located in the microJECT), an analog timer and a digital pressure display.
External valve (slow, switch time >10 ms) 3.3. Description of the Front Panel Figure 5: PDES-01DM front panel view (the numbers are related to those in the text below) (1) Manometer Digital display for the pressure (X.XX bar or XXX.X psi)
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Connector for connecting the filtered, dry and oil-free gas source. The gas source must be filtered by a 5 µm filter element. Important: Do not connect sources of OXYGEN or COMBUSTIBLE gases!! Note: npi recommends an input pressure of 1 bar / 14 psi more than the maximum output pressure. (5) Pressure regulator Rotary knob for regulating the pressure of the ejection channel.
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PDES-01DM User Manual (12) Operation mode switch Switch for setting the operation mode TIMER: The internal timer is used. The timer can be started via a TTL HIGH signal at #11 or by pushing a pushbutton connected to #10 CONT.:...
PDES-01DM User Manual 4. External Valves External valves for the PDES system are either mounted on a remote valve plate or in the microJECT micro valve pipette holder. 4.1. Remote Valve Plate The valve which is controlled by the PDES is located on the remote valve plate (see Figure 6).
PDES-01DM User Manual 4.1. microJECT micro Valve Pipette Holder The valve which is controlled by the PDES is located in the micro valve pipette holder, called microJECT (see Figure 7). Tubing and cable of the microJECT are connected at the front panel of the PDES system (refer to chapter 3.3).
PDES-01DM User Manual 4.2. Operation Connect the gas source (max. 5 bar) to the INPUT connector at the front panel. Important: Do not connect sources of OXYGEN or COMBUSTIBLE gases!! The gas source MUST be dry and oil free!! Please use a degreaser and filter the gas source by a 5 µm filter element.
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PDES-01DM User Manual Figure 9: Operation modes of the PDES-01DM version 1.3 page 14...
PDES-01DM User Manual 5. Literature Bickmeyer, U., Heine, M., Manzke, T., & Richter, D. W. (2002). Differential modulation of Ih by 5-HT receptors in mouse CA1 hippocampal neurons. Eur.J.Neurosci. 16, 209-218. Eggermann, E. & Feldmeyer, D. (2009). Cholinergic filtering in the recurrent excitatory microcircuit of cortical layer 4.
PDES-01DM User Manual Microinjection into Oocytes Wetzel, C. H., Oles, M., Wellerdieck, C., Kuczkowiak, M., Gisselmann, G., & Hatt, H. (1999). Specificity and sensitivity of a human olfactory receptor functionally expressed in human embryonic kidney 293 cells and Xenopus Laevis oocytes. J.Neurosci. 19, 7426-7433.
PDES-01DM User Manual 6. Technical Data Source gas: air, nitrogen, helium etc. no aggressive, combustible or explosive gases, filtered by a 5 µm filter element Pressure range: input pressure: max. 5 bar (1 bar = 10 Pa = 14.5 psi)
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