Jasco RI-2031 Instruction Manual
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Jasco RI-2031 Instruction Manual

Intelligent refractive index detector
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MODEL RI-2031
INTELLIGENT REFRACTIVE INDEX
DETECTOR
INSTRUCTION MANUAL
P/N: 0302-0555A

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Summary of Contents for Jasco RI-2031

  • Page 1 MODEL RI-2031 INTELLIGENT REFRACTIVE INDEX DETECTOR INSTRUCTION MANUAL P/N: 0302-0555A...
  • Page 2 Safety Considerations To ensure operation safety, this instrument must be operated correctly and maintained regularly according to schedule. Carefully read to fully understand all safety precautions in this manual before operating the instrument. This manual denotes precautions against actions that can result in hazardous situations or equipment damage by using the signal words WARNING, CAUTION, and Note.
  • Page 3 Warning labels are attached at several locations on this instrument. Do not remove, deface or damage the warning labels. If a warning label peels off the instrument or becomes illegible, contact your local JASCO distributor for a replacement label. Be sure to indicate the part number (P/N) on the label.

  • Page 4: Regulatory Statements

    – Part 1:General requirements" • A "Declaration of Conformity" in accordance with the above standards has been made and is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (LC), Italy. FCC Statement (for USA only)

  • Page 5: Preface

    This manual should be easily accessible to the operator at all times during instrument operation. When not using the instrument, keep this manual stored in a safe place. Should this instruction manual be lost, order a replacement from your local JASCO distributor.

  • Page 6: Installation Conditions

    Installation Conditions To ensure safe operation, the following recommendations should be observed: Do not operate the instrument under voltage fluctuations exceeding 10% of the recommended line voltage. Large fluctuations may cause the instrument to fail. Use a three-pronged electrical outlet with a ground. When only a two-pronged socket is available, use an adapter and be sure to connect the ground wire of the adapter.

  • Page 7: Maintenance

    (2) Software prohibitions: Copying of software or related materials for purposes other than backup is prohibited. Transfer or authorization of the use of Jasco software to or by a third party is prohibited. Disclosure of confidential information related to Jasco software is prohibited.

  • Page 8: Warranty

    This product is warranted for a period of one year from the date of delivery. If any defects should occur in the product during this period of warranty, JASCO will repair or replace the defective part(s) or product free of charge.
  • Page 9 viii...

  • Page 10: Table Of Contents

    Table of Contents Safety Considerations ..............i Regulatory Statements ..............iii Preface..................iv Installation Conditions ..............v Maintenance .................vi Warranty ..................vii Table of Contents.................ix Outline and Specifications...........1 1.1 Outline ....................1 1.2 Specifications ..................2 Part Names and Functions ..........4 2.1 Front panel ..................... 4 2.2 Operation panel ..................
  • Page 11 4.5 Measurement of concentrated sample (Refractive index range shifting....................19 [SHIFT] Key Operations .............21 5.1 Operation summary................21 5.2 Preamplified output display ([SHIFT][1]) ........... 22 5.3 Auto-zero operation setting ([SHIFT][3])..........23 5.4 Zero point shift for integrator output ([SHIFT][4])......24 5.5 Lamp operation time ([SHIFT][5]) ............
  • Page 12 Maintenance................45 9.1 Error messages and countermeasures..........45 9.1.1 Errors during self-diagnostics after power on......45 9.1.2 Self-diagnostics during operation ..........46 9.1.3 Error message meanings and countermeasures....... 46 9.2 Flow cell bubble removal ..............48 9.2.1 Baselines when bubbles are generated ........48 9.2.2 Bubble removal method 1 (high flow rate fluid pump)....

  • Page 14: Outline And Specifications

    Using LC-Net, parameters can be set from the Model HSS-2000 System without an interface. Compact design The front panel dimensions are the same as other JASCO 2000 series HPLC equipment (pump, UV detector), which facilitates a neat stacking arrangement. Safety measures An internal fluid leak sensor is installed.

  • Page 15: Specifications

    1.2 Specifications Model name: RI-2031 Intelligent Refractive Index Detector Measurement system: Deflection type Refractive index range: 1.00 ~ 1.75 Measurement range settings: 1/4, 1/2, 1, 2, 4, 8, 16, 32, 64, 128, 256, 512 x RIU/full scale (“MEASURE RANGE”: STD)
  • Page 16 Time program: Parameters that can be set are PURGE ON/OFF, AUTO ZERO, RANGE, RESPONSE. Range from 0.1 to 999.9min. Response FAST, STD, SLOW (TIME ACCUME, DIGITAL FICTER) Input and output signals: Recorder output: 10mV/FS Integrator output: 5.0 x 10 RIU/1V(SID) 5.0 x 10 RIU/1V(WIDE) Marker and leak output:...

  • Page 17: Part Names And Functions

    Part Names and Functions 2.1 Front panel Operation Panel Solvent Inlet and Outlet POWER Switch   Figure 2.1 Front Panel Name Function Solvent Inlet Outlet “1” ~ “6” Sample and Reference In and out connections Refer to Figure 2.2 and Figure 2.3. Operation panel Panel containing control keys, parameter edit keys, and LCD display showing operating conditions, parameters...
  • Page 18 Note: For a “10 ~ 50mL/min” tubing arrangement, the refractive index signal output (and display) polarity becomes negative. Change the polarity setting from plus (+) to minus (-) using the polarity setting function. (Refer to 4.1.3 polarity setting.) Sample Out (waste) Tubing with Flow rate:...
  • Page 19 Reference IN Sample Out Tubing OD=1.6mm ID=0.8mm (Same tubing as used to connect ports Flow rate: 10 50mL/min. No.1-2 or ports No.4-5. for the “ 10mL/min” tubing arrangement. Reference Out Sample IN Tubing OD=1.6mm, ID=0.8mm Sample Out Tubing Mobile phase OD=1.6mm ID=0.8mm Flow rate: 10...

  • Page 20: Operation Panel

    2.2 Operation panel   Figure 2.4 Operation Panel Name Function LCD screen Displays operation conditions, setting values, error messages and other information. [PRGM RUN] Used to start and stop the time program. Operation status is indicated by the lamp to the upper left of the button. The lamp is lit during program operation.

  • Page 21: Back Panel

    2.3 Back panel Input/Output Terminals LC-Net Connectors RS-232C Terminal Fuses AC input Ground Terminal Figure 2.5 Back Panel...
  • Page 22 Name Function Input and output terminals Signal output terminals REC+/- Recorder output terminal (Output level G(GND)- determined by RANGE setting) INT+/- Integrator output terminal (Fixed scale of G(GND) 5x10 RIU/1V(“MEASURE RANGE”: STD), 5x10 RIU/1V(“MEASURE RANGE”: WIDE). MARK OUT +/- Marker output terminal (Normally open, contact type output is closed when [MARK] key is pressed or MARK IN is input) LEAK OUT +/-...

  • Page 23: Power On/Off And Self-Diagnostics

    Power On/Off and Self-diagnostics 3.1 Power on and self-diagnostics Turn the power switch, located at the lower left of the front panel, to the ON position. The self-diagnostics program is automatically executed. The program examines the following items: ROM CHECK (Memory) RAM CHECK (Memory) DC POWER (Direct current power source) BACK UP (Memory backed up by battery)

  • Page 24: Power Off

    Current Optical bench Response Sensitivity Mode display temperature/setting (64) (standard) (Normal operation mode) temperature MODE RANGE RESPONSE MODE RANGE RESPONSE Temperature [MONIT] TEMP: xx.x/yy   NORM °C control mark blinks -0.1 -0.1 when heating   RIU(x10 ) POLARITY PURGE RIU(x10 ) POLARITY PURGE Current Purge valve status Polarity...

  • Page 25: Operations In Normal Operation Mode

    “MEASURE RANGE” to WIDE. Refer to section 7. The maximum flow rate of the RI-2031 depends on the tubing connections of the front cell panel. The “ ~ 10mL/min.” tubing connections are for conventional analysis and the “10 ~ 50mL/min”...
  • Page 26 Input range: 1/4, 1/2, 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, S (Short) x10 RIU /10mV (“MEASURE RANGE”: STD) 1/4, 1/2, 1, 2, 4, 8, 16, 32, 64, 128, 256, 512, S (Short) x10 RIU /10mV (“MEASURE RANGE”: WIDE) Response input range: FAST, STD, SLOW (1) Change method 1 NORM 64(16) STD(FAST)
  • Page 27 NORM 64 STD(FAST) NORM 64(16) STD -0.000 -0.000 ][EDIT/ENTER] [RANGE/4] ][EDIT/ENTER] [RSPNS/5]     Sensitivity flashes Response flashes NORM NORM -0.000 -0.000 Sensitivity change Response change Figure 4.3 Change Method (2)

  • Page 28: Temperature Control On/Off And Temperature Setting

    4.1.2 Temperature control ON/OFF and temperature setting When the normal operation monitor screen (2) (right in Figure 4.1) is displayed, temperature control can be turned ON/OFF and temperature can be set as shown the operations in Figure 4.4. Input range: 0 ~ 45°C (Operating temperature range: Room temperature + 10°C ~ Room temperature +25°C) 0: OFF (Temperature control not active) Note:...
  • Page 29 When the actual negative peak signal exceeds -5.0x10 RIU, polarity should be set to positive (+). Refer to Table 4.2 (“10 ~ 50mL/min.” tubing connections) 4.1.3.2 Negative peak signal exceeds the integrator input range The allowable input voltage range of a current integrator is, in general, -10mV ~ +1V.

  • Page 30: Purge Valve Open/Close (Solvent Replacement In The Reference Cell)

    RIU:20.0 ＋ RIU:10.0 RIU:-10.0 − RIU:-20.0 Polarity:Negative Actual refractive index change Polarity:Positive Figure 4.5 Changes in Peak Appearance and Display Values Caused by Polarity Differences NORM Normal operation mode monitor screen(1) -0.0 [SHIFT] [5] [MONIT] [EDIT/ENTER] POLARITY POLARITY +(-) ][EDIT/ENTER]  ...

  • Page 31: Auto-Zero Operations

    Normal operation mode monitor screen [MONIT] NORM TEMP: xx.x/yy °C   -0.0 + ON(OFF) -0.0 + ON(OFF)   [PURGE] [MONIT] Current purge valve status PURGE Purge valve open/close screen 1:ON 0:OFF :Opens the purge valve and changes the status to ON. :Closes the purge valve and changes the status to OFF.

  • Page 32: Measurement Of Concentrated Sample

    monitor screen. If the problem continues, the same message will appear again after approximately 20 seconds. Refer to section 9.1 for countermeasures. Table 4.3 Error Messages and Their Meanings Error message Meaning TROUBLE LEAK IN CELL Fluid is leaking from the flow cell TROUBLE OVER TEMP Heater is overheating TROUBLE ZERO GLASS DRV...
  • Page 33 range is exceeded, use the recorder output. The refractive index range resulting in proper recorder output is +/-9.9x10 RIU (with a range setting of 512). However, signal linearity is lowered when above +/- 5.0 X 10 RIU. Note: When the “MEASURE RANGE” is set to WIDE, this refractive index range shifting method is invalid.

  • Page 34: Shift] Key Operations

    [SHIFT] Key Operations 5.1 Operation summary The operations executed using the [SHIFT] key are summarized in Table 5.1. Table 5.1 [Shift] Key Operations and their Functions Key operation Function [SHIFT][1] Preamplified output display (refer to section 5.2). Used to check for bubbles in the cell and when adjusting optical zero.

  • Page 35: Preamplified Output Display ([Shift][1])

    ・Marker voltage/polarity change (refer to section 5.7.1) ・  Setting to continue/stop self-diagnostics when an error occurs (refer to section 5.7.2) ・Serial number setting (refer to section 9.6) 5.2 Preamplified output display ([SHIFT][1]) Preamplified output is used to check for bubbles in the cell and when adjusting optical zero.

  • Page 36: Auto-Zero Operation Setting ([Shift][3])

    present, transparency is reduced and the value decreases. If the value falls below 0.1V, the INTENSITY POOR message appears. (2) Optical zero-adjustment When “MEASURE RANGE ” is set to STD, be sure to execute optical zero adjustment after solvent replacement. Even when using the same solvent, readjust whenever new solvent is replaced.

  • Page 37: Zero Point Shift For Integrator Output ([Shift][4])

    AUTO When analyzing samples using an autosampler, the MARK IN signal, input to the detector from the autosampler at the time of sample injection, causes a marker to be overlayed on the chromatogram and auto-zero to be executed. The chromatogram base is therefore set to zero. MANUAL Auto-zeroing is not executed when a signal is input into the MARK IN terminal.
  • Page 38 Note 1: The zero point shift setting has no affect on recorder output. Note 2: The extent of integrator output noise is not changed by the zero point shift setting. However, as the input voltage increases, the signal resolution of the integrator decreases and visible baseline noise increases.

  • Page 39: Lamp Operation Time ([Shift][5])

    In t e g r a t o r o u t p u t Z e r o p o i n t s h i ft : 0 m V - 1 0 m V In t e g r a t o r p r o c e s s i n g n o t p o s s i b l e In t e g r a t o r o u t p u t Z e r o p o i n t s h i ft : + 5 0 m V Figure 5.4 Example when Baseline Drifts in Negative Direction...

  • Page 40: Signal Filtering Mode ([Shift][7])

    5.6 Signal filtering mode ([SHIFT][7]) The signal filtering method is normally set to “TIME ACCUME”. If a high noise level makes analysis using the “TIME ACCUME” method difficult, switch to the “DIGITAL FILTER” method. Generally, the “DIGITAL FILTER” method decreases noise, but peak shape may be broad. Select the adequate filtering method and response setting in relation to the chromatogram characteristics.
  • Page 41 ・  Constant K1 (for STD side optics calibration constant) setting (refer to section 9.4.5) ・  Constant K2 (for WIDE side optics calibration constant) setting (refer to section 9.4.5) ・  Calibration (refer to section 9.4) ・  Lamp maintenance time setting (refer to section 9.5) ・Marker voltage/polarity change (refer to section 5.7.1) ・ ...

  • Page 42: Marker Voltage/Polarity Changes

    Normal operation mode monitor screen [MONIT] NORM TEMP: xx.x/yy °C      -0.0 -0.0   [MONIT] [SHIFT] [MARK] PARAMETER MODE Measurement range setting menu 1:MEASURE RANGE PARAMETER MODE Constant K1 setting 2:SET K1 VALUE PARAMETER MODE Constant K2 setting 3:SET K2 VALUE PARAMETER MODE K1, K2 Calibration 4:CALIB.

  • Page 43: Setting To Continue/Stop Self-Diagnostics When An Error Occurs

    The procedures required to change the settings outlined in Figure 5.8. Marker voltage input range: 0, 0.5, 1, 2mV Normal operation mode monitor screen [MONIT] TEMP: xx.x/yy °C    NORM   -0.0 + ON(OFF) -0.0 + ON(OFF)   [MONIT] [SHIFT] [MARK] (Display the menu below by pressing the key twice) Marker voltage/polarity menu...
  • Page 44 Input range: CONFIRM (Stop), SKIP (Continue) Normal operation mode monitor screen [MONIT] TEMP: xx.x/yy °C    NORM   -0.0 + ON(OFF) -0.0 + ON(OFF)   [MONIT] [SHIFT] [MARK] (Use the keys to display the menu below) Menu to continue/stop self- diagnostics when an error occurs.

  • Page 45: Program Mode Operations

    Program Mode Operations 6.1 Operation summary In program mode the range, response, valve opening and closing, and auto-zero operations can be changed according to a time program. The maximum number of steps in the time program is 64 and the maximum number of program files that can be stored is 10.

  • Page 46: File Number Setting (File Loading)

    Normal operation mode monitor screen [MONIT] TEMP: xx.x/yy °C        NORM   -0.0 -0.0   [PRGM] [MONIT] [MONIT] PRG2 T:0.0 PRG2 TEMP: xx.x/yy °C        -0.0 -0.0 -0.0   Program mode monitor screen Figure 6.1 Switching between Normal Operation Mode and Program Mode The contents of each screen are described in Figure 6.2.

  • Page 47: Program Editing

    Program mode monitor Normal operation screen mode monitor screen [PRGM] [MONIT] [SHIFT] [PRGM] [EDIT/ENTER] RPOGRAM FILE NO. RPOGRAM FILE NO. y(x) [x][EDIT/ENTER] (X) is the value after the change Flashes File number screen Input screen Figure 6.3 File Number Change Note 1: If an incorrect value is entered accidentally, press the [CLEAR] key before pressing the [EDIT/ENTER] key then enter the value again.

  • Page 48: Time Program Editing

    Program mode monitor screen [MONIT] [MONIT] PRG2 TEMP: xx.x/yy°C PRG2 T:0.0   -0.0 -0.0 -0.0   [MONIT] PRG2 INITIAL Initial parameter screen Note: Values in parentheses are R:64(16) PURGE:OFF(ON) after changes Purge valve Sensitivity [EDIT/ENTER] PRG2 INITIAL Sensitivity input screen R:64 PURGE:OFF Sensitivity [EDIT/ENTER]...
  • Page 49 PRG2 INITIAL Initial parameter screen R:16 PURGE:OFF ▼ Step 2 screen Step 1 screen PRG2 ST 1 T:0.0 ST２ T:0.0           [EDIT/ENTER] [EDIT/ENTER] Time flashes ST 1 T:0.0 Time flashes ST 2 T:0.0 (waiting for input) (waiting for input) Input 1 minute [1][EDIT/ENTER] [2][EDIT/ENTER]...
  • Page 50 [xx][EDIT/ENTER] Function will flash next. d) Input the function. Select a function from Table 6.1. For example [RANGE/4][EDIT/ENTER] The input function will appear and the value will flash. e) Input a value. [ ][ ][EDIT/ENTER] When input is complete, press the [ ] key to move to the next step. Repeat the operations in steps a) ~ e) to edit the time program.
  • Page 51 [SHIFT][ ]: Moves to the last step in the program [SHIFT][ ]: Moves to the first step in the program 6.4.2.2 Corrections When time and functions do not agree 1) Use the [ ] key to display the incorrect step. 2) Re-input the step values starting from time.
  • Page 52 PRG2 ST 3 T:1.0     RANGE : 16   [EDIT/ENTER] PRG2 ST 3 T:1.0   Time flashes (waiting for input)   RANGE : 16   [1][EDIT/ENTER] Time input ST 3 T:1.0 Function flashes [EDIT/ENTER] (waiting for input) 1: PURGE OFF [RANGE/4][EDIT/ENTER] Sensitivity call up PRG2...

  • Page 53: Time Program Operation

    3) Press the [MONIT] key to return to the monitor screen. 6.4.2.4 Insertion 1) Display the final step in the program using [SHIFT][ ]. 2) Display a new step using the [ ] key. 3) Follow the procedure described in section 6.4.2.1 and input desired values. 4) Press the [MONIT] key to return to the monitor screen.

  • Page 54: Measure Range Setting

    MEASURE RANGE Setting One of the major features of the RI-2031 is that the dynamic measurement range is 10 times wider than an ordinary RI detector. The “MEASURE RANGE” setting is normally set to STD, however when measuring a high concentration sample, “MEASURE RANGE”...
  • Page 55 Note: Although signal linearity is reduced gradually when the refractive index value exceeds 5x10 RIU (“MEASURE RANGE”: STD) or 5x10 RIU(“MEAUSRE RANGE”: WIDE), the measurable range is ~ 7x10 RIU/1.0V (“MEASURE RANGE”: STD ), and ~ 9.9x10 RIU/1.0V (“MEASURE RANGE”: WIDE). When exceeding the linear range, connect to the recorder output terminal instead of the integrator output terminal.

  • Page 56: Maximum Flow Rate Changing At The Front Panel

    Maximum Flow Rate Changing at the Front Panel The maximum flow rate depends on the front panel tubing arrangement. The following subsections describe tubing arrangements and the method for replacing solvent in the reference cell. 8.1 “ ~ 10mL/min” tubing arrangement and solvent replacement method for reference cell Connects ports No.1 and No.2 with outer diameter: 1.6mm, internal diameter:0.8mm tubing and ports No.4 and No.5 with outer diameter:1.6mm,...

  • Page 57: 10 ~ 50Ml/Min" Tubing Arrangement And Solvent Replacement Method For Reference Cell

    8.2 “10 ~ 50mL/min” tubing arrangement and solvent replacement method for reference cell Connect the tubing from the mobile phase solvent bottle to port No3. Use tubing with an internal diameter of 0.8mm and an outer diameter of 1.6mm. Connect the syringe to port No.6. To replace solvent in the reference cell, draw up mobile phase solvent by pulling the syringe plunger.

  • Page 58: Maintenance

    3 minutes, and turn the power on again. If the re-input values have changed again, the backup battery is dead and the user should contact your local JASCO distributor.

  • Page 59: Self-Diagnostics During Operation

    WARNING LAMP MAINTE TIME 9.1.3 Error message meanings and countermeasures 9.1.3.1 TROUBLE LEAK IN CELL This message appears when fluid is leaking from the flow cell. Immediately stop the pump, turn the power off, and contact your local JASCO distributor.
  • Page 60 The message may also appear when misjudgment happens due to a problem with the temperature detection system. Immediately turn the power off and contact your local JASCO distributor. 9.1.3.3 WARNING INTENSITY POOR This message appears when the light intensity incident on the photodiodes is insufficient.

  • Page 61: Flow Cell Bubble Removal

    value. The following problems are possible when the message appears. (1) The solvent on the sample side is different from the solvent on the reference side. (2) Large bubbles remain in the cell -> Remove the bubbles (refer to section 9.2).
  • Page 62 (1) Irregular noise (Figure 9.1) (2) Increased drift (Figure 9.2) (3) Irregular ghost peaks (Figure 9.3) When the above phenomena occur, display the preamplified output screen and check the SUM value (refer to section 5.2). If the third digit after the decimal point of the SUM value is fluctuating, it is possible that bubbles exist in the flow cell.

  • Page 63: Bubble Removal Method 1 (High Flow Rate Fluid Pump)

    When using a “ ~ 10mL/min.” tubing arrangement, connect the pump directly to the RI-2031, then open and close the purge valve (ON/OFF) (refer to section 4.2) while pumping fluid at a high flow rate (up to 10mL/min is possible).

  • Page 64: Flow Cell Cleaning Method

    (1) Use a degasser. (2) Sufficiently check for clogs in the pump inlet filter. When the inlet filter is clogged, bubbles easily enter the flow cell. (3) Place the waste fluid bottle in a position higher than the instrument. 9.3 Flow cell cleaning method Note1: Reversing the fluid flow direction is prohibited.

  • Page 65: Calibration Method

    Figure 9.4 Flow Cell Cleaning Method 9.4 Calibration method A sophisticated signal processing system incorporated into the RI-2031 is able to measure refractive index accurately without being affected by variations or fluctuations in lamp emission, or by the light absorption caused by a mobile phase solvent or a sample solute.

  • Page 66: Tubing

    1.0mm. Change to the “ ~ 10mL/min.” tubing configuration. (refer to section 8) Integrator or recorder Port No.3 1mL loop RI-2031 Pump Injector Internal diameter of 0.25mm or less Port No.6 Water Figure 9.5 Tubing and Wiring Arrangement for Calibration 9.4.2 Standard solution...

  • Page 67: Replacement Of Tubing System Solvent With Water

    9.4.3 Replacement of tubing system solvent with water Note: When the solvent in the cell is not miscible with water, first replace with an intermediate solvent then replace with water. For example, when the solvent in the cell is chloroform or THF (oxidation inhibitor is insoluble in water), first replace with acetone then replace with water.
  • Page 68 Normal operation mode monitor screen [MONIT] NORM TEMP: xx.x/yy °C          -0.000 + ON(OFF) -0.000 + ON(OFF)     [SHIFT] [MARK] [MONIT] ][EDIT/ENTER]     PARAMETER SET   4:CALIB K   [MONIT] [EDIT/ENTER] K CALIB: xxxx.x   Calibration screen  ...

  • Page 69: Calibration Constant Confirmation And Setting

    Plateau  Figure 9.7 Peak Shape Note: When the peak dose not contain a plateau, inspect the internal diameter of the tubing from the injector to the detector. When the plateau is short, decrease the flow rate. (5) Repeat the operations in steps (2) ~ (4) and confirm that the RIU value is within 160.0 ±...

  • Page 70: Alternative Calibration Method

    (2) When the backup battery has deteriorated and the K1 value has reverted to 1.00. (3) When the PCB (CPU board) has been replaced Note: At the time of shipping, the K1 and K2 values are recorded on a label attached inside the instrument.

  • Page 71: Lamp Maintenance Time Setting

    LAMP MAINTENANCE warning will be displayed. Since lamp replacement is done by service engineer, contact your local JASCO distributor when this is required. When the lamp maintenance time is set to 0, the warning is not displayed. In addition, when BACKUP ERROR is displayed due to deterioration of the battery, the lamp maintenance time recorded in RAM will be lost.

  • Page 72: Serial Number Setting

    RAM will be lost. When this occurs, use the procedure outlined in Figure 9.10 to re-input the serial number. When connecting to a JASCO system controller via LC-Net, the serial number is displayed on the HPLC system configuration screen.

  • Page 73: Power Fuse Replacement

    Normal operation mode monitor screen [MONIT] TEMP: xx.x/yy°C         NORM -0.0 -0.0   [MONIT] [SHIFT] [MARK] keys to display the menu below) (Use the Serial number menu PARAMETER MODE 6:SERIAL NO. [MONIT] [EDIT/ENTER] [EDIT/ENTER] SERIAL NO SERIAL NO 1234567890 1234567890 [xxxxxxxxxx] [EDIT/ENTER]...
  • Page 74 (1) Pull the old fuses out from fuse holder, insert new fuses, and insert the fuse holder back into its original position. If the replaced fuses immediately blow out again, an instrument problem is possible. Contact your local JASCO distributor. Fuse Holder Figure 9.11 Fuse Holder...

  • Page 75: Appendix

    10. Appendix 10.1 Operation Theory 10.1.1 Operation theory of deflection differential refractometers With deflection differential refractometers, light refracted inside the prism flow cell is used to detect the difference in refractive indices between the reference (solvent) and the sample. The flow cell structure and light path are shown in Figure 10.1. θ...

  • Page 76: Optical System

    10.1.2 Optical system Collimator lens Slit Second photodiode       Light source   Half mirror   Flow cell   Zero glass   (motor driven) First photodiode       Figure 10.2 Optical System Light from the light source passes through a slit and into the collimator lens where it is made into a parallel beam.
  • Page 77 PD1 output PD2 output No difference between refractive indices PD1 output PD2 output Difference between refractive indices Figure 10.3 Light Image on Photodiodes First, when there is no difference between the refractive indices of the reference (solvent) and the sample, the light image overlaps PD1 and PD2 equally as shown in the top half of Figure 10.3.

  • Page 78: Signal Processing Method

    Therefore, the value is not affected by light source deterioration or flow cell contamination. The RI-2031 measurement system uses two calibration constants (K1 and K2). K1 value is for analytical measurement (first photodiode) and K2 value is for...

  • Page 79: Flow Line

    10.1.4 Flow line A flow line diagram for the “ ~ 10mL/min.” tubing arrangement, is shown in Figure 10.4. Effluent form the column is introduced from the port No.6 and waste is discharged from the port No.3. Flow lines are changed using the purge valve.
  • Page 80 A flow line diagram for the “10 ~ 50mL/min.” tubing arrangement, is shown in Figure 10.5. Effluent from the column is introduced from the port No.5 (Sample IN) and waste is discharged from the port No.2 (Sample OUT). The mobile phase solvent is introduced from the port No.3 (Reference IN) and is drawn using the syringe, from the port No.6 (Reference OUT).

  • Page 81: Internal Volume Of Tubing

    10.1.5 Internal volume of tubing Internal volume of tubing is described in Table 10.1 and Table 10.2. Table 10.1 Internal volume using “ ~ 10mL/min.” tubing Flow line Volume Port No6 -> Sample cell ca. 70 µL Sample cell -> Port No3 ca.

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