Table of Contents
Summary of Contents for Okolab H301-NIKON-TI-SR
- Page 1 User Manual H301-NIKON-TI-SR March 2015 H301-NIKON-TI-SR Compatible with the following XY stages Compatible with the following Okolab Controllers Nikon T/Ti-SR H301-T-BL-PLUS UNO-COMBINED-CONTROLLER H401-T-DUAL www.oko-lab.com...
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Page 2: Table Of Contents
User Manual H301-NIKON-TI-SR March 2015 Index Components and dimensions....................3 Sample Holders ........................3 2.1 Available Sample Holders ....................... 3 Available Lids ........................4 3.1 Sliding lid .......................... 5 3.2 Koehler Lid ........................6 3.3 Injection Lid ........................6 3.4 Laser Interlock Lid ....................... 7 Insertion of the Sample Feedback Temperature Sensor .............. -
Page 3: Components And Dimensions
Sample Holder-Order Separately Integrated Humidty Module Chamber top view Chamber Base Figure 1. H301-NIKON-TI-SR - Components and Dimensions. 2. Sample Holders Available Sample Holders The following sample holders are available. NOTE: Please contact info@oko-lab.com... -
Page 4: Available Lids
3. Available Lids The following heated glass Lids are available for H301-NIKON-TI-SR : H301-SLIDING-LID: For easy sample loading and pipetting – Included in code H301-NIKON-TI-SR H301-KOEHLER-LID-2*: Reduces chamber height to 23 mm and allows imaging under Koehler illumination ... -
Page 5: Sliding Lid
A in image 2. Open Sliding Lid to access screws labeled A in image 3. Sliding Lid Distance between focal plane and upper side of the glass lid Chamber top view Figure 3. H301-NIKON-TI-SR (Sliding Lid comes as standard) Sliding Lid Screws M2x6 Figure 4. Assembly of the sliding lid www.oko-lab.com... -
Page 6: Koehler Lid
Distance between focal plane and upper side of the glass lid Chamber top view Figure 5. H301-NIKON-TI-SR + H301-KOEHLER-LID-2 Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma- nent access to the sample. -
Page 7: Laser Interlock Lid
Distance between focal plane and upper side of the glass lid Chamber top view Figure 6. H301-NIKON-TI-SR + H301-INJECTION-LID Laser Interlock Lid The Laser Interlock Lid is a heated glass lid with a safety switch connecting to the laser controller. It automatically turns the laser off when the lid is lifted. -
Page 8: Insertion Of The Sample Feedback Temperature Sensor
H301-NIKON-TI-SR March 2015 4. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-NIKON-TI-SR (see Figure 8, Frontal and 3D views). Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view... -
Page 9: Working With 35 Or 60 Mm Petri Dish - Spacing Rings And Magnetic Locks
User Manual H301-NIKON-TI-SR March 2015 6. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting holder’s height. - Page 10 User Manual H301-NIKON-TI-SR March 2015 Ibidi μ-Dish35mm-low Grainer-Petri35x10 Corning-35x10mm MatTek-P35G-X-14-X BD Falcon-35x10 Willco-35x10 Figure 12. Ring selection scheme for 35 mm dish. Ibidi μ-dish 50mm-low MatTek-P50G-X-30-X Grainer - Petri 60x15 BD Falcon 60x15 Corning-60x15mm Willco60x15 Figure 13. Ring selection scheme for 60 mm dish.
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Page 11: Working With 1X3'' And 1X2'' Chamber Slides - Magnetic Locks
User Manual H301-NIKON-TI-SR March 2015 7. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. NOTE: Magnetic locks are included with sample holder. -
Page 12: Connecting The Gas Supply And Filling The Humidity Module
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-NIKON-TI-SR. Silicon tubing con- nects to a gas input - brass opening - located on a side of the H301-NIKON-TI-SR. See Figure 16. Connect the silicon tubing by gently pushing it onto brass opening. -
Page 13: Connecting The Chamber With Xy Stage
User Manual H301-NIKON-TI-SR March 2015 10. Connecting the Chamber with XY stage Follow the steps shown in the images Figure 18 and listed below in order to correctly connect the chamber with XY stage. Place the chamber on the stage and tighten 4 captive screws (See Figure 18 Image 1 and Image 2). Captive screws housings are indicated with letter B in Figure 18 Image 2 Use a 2.5mm metric Allen Wrench to tighten the four captive screws while keeping the chamber sliding lid open...