Table of Contents
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1. Overview
The Model 210D front panel houses the UV detector lamp switch, Rheodyne injection valve, prime/purge
valve, pressure gauge, and the storage
compartment. In the storage compartment
are the LED display for the column
temperature, and the vacuum degas pressure
gauge (shown above). An accessories kit is
included with your 210D. In the kit are two
PEEK finger tight nuts (Alltech part # 32233)
for column connection, a spare 1/16"
stainless steel fitting (Valco part ZN1) and
ferrule (Valco part ZF1S6), a flange fitting
(Upchurch part 203X) and ferrule
(Upchurch part 240X) for the solvent recycle valve, a 20mL priming syringe (Sigma Aldrich part Z248037),
and a 100µL glass injection syringe (SGE part 005300).
On the right hand side of the 210D is the column compartment, which gives you access to the column heater,
pump head(s), solvent recycle valve, and the detector housing. Open the column compartment by loosening
the two captive thumbscrews and lifting off the cover. Use the picture below to familiarize yourself with the
interior of the column compartment.
Rheodyne 7725i
injection valve
with a 20µL
sample loop
Prime/purge
valve
Pressure
gauge
Pulse damper
Pump heads
UV detector
lamp switch
Storage
compartment
Pump outlet line
Pump inlet lines
Solvent recycle valve
Rheodyne 7725i injection valve
Column heater
Vacuum de-gas coil
HPLC
Model 210D
Prime/purge
valve
Column
compartment
thumbscrews
Pressure gauge
UV and
Conductivity
detectors
Vacuum
de-gas
outlet
Vertical
slide
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Table of Contents
Summary of Contents for Sri 210D
- Page 1 100µL glass injection syringe (SGE part 005300). On the right hand side of the 210D is the column compartment, which gives you access to the column heater, pump head(s), solvent recycle valve, and the detector housing. Open the column compartment by loosening the two captive thumbscrews and lifting off the cover.
- Page 2 HPLC Model 210D 2. Installing a Column Slide the column heater up Slide the column heater up. on its aluminum slider. Install the column of your choice using the included PEEK finger tight nuts. Slide the nuts onto the PEEK tubing, letting about 1mm of PEEK tubing protrude.
- Page 3 Piston flush outlet line Piston flush IN Pump outlet High pressure seal 210D pump, side view: Low pressure seal Piston It is possible for solvent to leak past the high pressure seal with the back and forth thrusting of the piston.
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Page 4: Vacuum De-Gas
Power supply jack 6. Power Supply Your 210D comes with a universal (115V or 230V) benchtop 12 volt power supply (shown at right). Plug the small power source cord into the jack on the left hand side of the LC. Plug the larger power cord into a wall outlet. - Page 5 210D is printed on the HPLC under the cable port, and also on your PeakSimple disk. Once you have successfully gotten the data system to sign on, click Save All so you don’t have to repeat this step the next time...
- Page 6 C, which is suitable for a wide range of common applications. Note for those who have the 4 or 6 channel data system upgrade: Unless you have connected an external detector to the 210D, the column temperature will display on channel 3, and the detector cell temperature will display on channel 4.
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Page 7: Priming The Pump
HPLC Model 210D Click and choose . In the Relays/pump window, click on Relay A to turn the View Relay/pump window pump OFF, since you haven’t primed it yet. The flow rate of the pump (pump A in a binary gradient system) can be adjusted from 0.1 to 5.0mL/minute, in increments of 0.1mL/minute, using the dialog box at the bottom... - Page 8 20µL sample loop. Leave the syringe in the while you turn the Rheodyne injection valve knob to the INJECT position. The run will automatically start. See the Rheodyne documentation in your SRI manual under HPLC Injectors for more information about operating the injection valve.
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Page 9: Expected Performance
HPLC Model 210D Expected Performance This chromatogram shows the 210D UV detector response to a BTX sample (benzene, toluene, and ortho xylene). Description: UV 254 Sample: 20µL 100ppm BTX Column: 15cm C18 5µ Mobile phase: 70% Isopropyl Alcohol, 30% water at 1.0mL/minute... - Page 10 Model 210D Expected Performance The first chromatogram is a UV separation of 5ppm BTX with an isocratic 210D system. The second chromatogram shows the same run after using PeakSimple’s data smoothing function. Short term up/down noise is a function of pump refill. Binary systems are therefore noisier than isocratic systems. PeakSimple’s smoothing feature can reduce the pump noise for a clearer picture of your data.
- Page 11 Expected Performance UV Detector Noise This pair of chromatograms shows a short-term, one minute UV detector noise run on an isocratic 210D system. The first chromatogram is the raw data, and the second shows it after smoothing. UV detector noise...
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Page 12: General Information
Short term up/down noise is a function of pump refill. Most other manufacturers smooth data inside detector. SRI does not, so pump noise is visible. Binary systems are therefore noisier than isocratic systems. PeakSimple’s smoothing feature can reduce the pump noise so you can more clearly picture your data. Pump noise peaks are much narrower than data peaks, and do not obscure, esp after smoothing is applied.
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