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Beckman Biomek 3000 Integration Manual

Shaking peltier device; laboratory automation workstation
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Shaking Peltier Device
Integration Manual
Biomek 3000 Laboratory Automation Workstation
Beckman Coulter PN A36985
Revision AC
February 2010
Beckman Coulter, Inc
250 S. Kraemer Blvd., Brea, CA 92821
Copyright © 2010 Beckman Coulter, Inc.

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  • Page 1 Shaking Peltier Device Integration Manual Biomek 3000 Laboratory Automation Workstation Beckman Coulter PN A36985 Revision AC February 2010 Beckman Coulter, Inc 250 S. Kraemer Blvd., Brea, CA 92821 Copyright © 2010 Beckman Coulter, Inc.
  • Page 2 Except as expressly provided herein, Beckman Coulter, Inc. makes no other warranty, whether oral or written, expressed or implied, as to any matter whatsoever, including but not limited to those concerning merchantability and fitness for a particular purpose, nor is freedom from any patent owned by Beckman Coulter, Inc. or by others to be inferred.

  • Page 3: Warranty And Returned Goods Requirements

    Warranty and Returned Goods Requirements All standard Beckman Coulter, Inc. policies governing returned goods apply to this product. Subject to the exceptions and upon the conditions stated below, the Company warrants that the products sold under this sales agreement shall be free from defects in...

  • Page 5: Safety Information

    Please pay special attention to the specific safety information associated with these symbols. WARNING: If the equipment is used in a manner not specified by Beckman Coulter, Inc., the protection provided by the equipment may be impaired. Warning and Caution Definitions All Warnings and Cautions in this document include an exclamation point, a lightning bolt, or a light burst symbol framed within a triangle.

  • Page 6: Electrical Safety

    To prevent electrically related injuries and property damage, properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies. Contact a Beckman Coulter Service Engineer for any servicing of equipment requiring the removal of covers or panels unless specifically stated otherwise.

  • Page 7: Chemical And Biological Safety

    Safety Information Chemical and Biological Safety Normal operation of the Shaking Peltier Device may involve the use of materials that are toxic, flammable, or otherwise biologically harmful. When using such materials, observe the following precautions: • Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease.

  • Page 8: Cleaning

    Beckman Coulter Service Engineer or returning parts to Beckman Coulter for repair. Beckman Coulter will NOT accept any items which have not been decontaminated where it is appropriate to do so. If any parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated.

  • Page 9: Warnings And Cautions Found In This Manual

    The WARNINGs and CAUTIONs found within this document are listed below. WARNING: If the equipment is used in a manner not speci- fied by Beckman Coulter, Inc., the protection provided by the equipment may be impaired. CAUTION: Use only the cables that were included with the Shaking Peltier Device.

  • Page 11: 安全信息

    安全信息 本文件中的所有 “警告”和 “当心”信息均附有惊叹号、闪电符号或三角形 内的闪光符号。请特别注意与此类符号相关的具体安全信息。 警告：如果以 Beckman Coulter,Inc. 未说明的方式使用器械，器械提供的保 护可能受到损害。 警告和当心定义 本文件中的所有 “警告”和 “当心”信息均附有惊叹号、闪电符号或三角形 内的闪光符号。 惊叹号符号是国际通用符号，用于提醒用户在尝试安装、使用、维护和修理之 前阅读和理解所有安全说明。 警告：警告提醒用户注意可能对操作员造成伤害的状况或可能的情形。 小心：当心提醒用户注意可能损坏或毁坏产品或操作员工作的状况或可能的 情形。 当本手册中显示该符号时，请特别注意与该符号相关的具体安全信息。 Shaking Peltier Device Integration Manual...

  • Page 12: 电气安全

    安全信息 电气安全 为了防止与电相关的受伤和财产损坏，所有电气设备在使用前需得到适当检 查，并且任何电气问题需立即报告。除非另有具体说明，如果任何设备维修需 要取下盖板或面板，请与 Beckman Coulter 维修工程师联系。 高压 这个符号指明存在高电压源带来的潜在触电危险；在所有模块的安装、维护及 修理前应通读和理解全部安全须知。除非有关指示明确要求取下系统盖板，请 勿取下这些盖板。为了避免触电，请只使用附带的电源线且插入已适当接地 （三孔）的墙壁插座。只使用制造商供应的多插座接线板。 电子设备弃置 理解和遵循所有有关电气设备安全和适当弃置的法规十分重要。 欧盟 《废弃电气和电子设备 （WEEE）指令》要求产品带有打叉轮箱符号。产 品上显示该符号表示： • 该设备于 2005 年 8 月 13 日之后投放欧洲市场。 • 该设备不得通过欧盟任何成员国的市政废品收集系统弃置。 如需获得有关 WEEE 指令要求产品的适当防污染和回收计划信息，请与您的 经销商或地方办事处联系，他们可向您提供适当的搜集、治疗、康复、设备回 收和安全弃置的信息。 Beckman Coulter, Inc.

  • Page 13: 化学与生物安全

    安全信息 xiii 化学与生物安全 设备的正常操作可能涉及使用有毒、易燃或在其他方面具有生物有害性的材 料。使用此类材料时，请遵守以下注意事项： • 依照妥善的实验室程序和方法处理传染性样品，以防传播疾病。 • 使用前遵守原溶液容器上印制的所有注意事项信息。 • 按照您所在设施的废品弃置程序弃置所有废溶液。 • 按照本手册中的说明操作设备，在使用病理性、有毒或放射性材料 时，采取所有必要的防范措施。 • 液体可能溅洒；因此，在使用具有潜在危险的液体时采取适当的安全 措施，例如佩戴安全镜和穿戴保护服。 • 在适当隔离的环境中使用有害材料。 • 在通电器械中或附近使用易燃液体时，应遵守安全管理人员规定的适 当防范程序。 • 在使用有毒、病理性或放射性材料时，应遵守安全管理人员规定的适 当防范程序。 附注：在设备操作过程中遵守所有附加或使用设备的警告和当心提示。查阅适 用的器械和设备使用手册中有关该设备的操作程序说明。 移动部件 为了避免移动部件造成伤害，请遵守以下规定： • 切勿在器械操作过程中尝试更换实验室器皿、反应物或工具。 • 切勿尝试以物理形式限制设备的任何移动部件。 • 保持工作场所无障碍物，防止阻碍设备移动。 Shaking Peltier Device Integration Manual...

  • Page 14: 清洁

    安全信息 清洁 遵守本用户手册中规定的清洁程序。清洁接触有害材料的器械之前： • 应与适当的化学和生物安全工作人员联系。 • 应查阅本用户手册中包含的化学和生物安全信息。 维护 仅限执行本手册中说明的维护程序。 本手册中未说明的维护程序应由 Beckman Coulter 维修服务代表执行。 在请求 Beckman Coulter 维修服务代表维修或将部件送回 Beckman Coulter 修理 重要事项 之前，您有责任对部件消毒。 Beckman Coulter 不接受需要消毒但尚未消毒的 任何物品。如果退回任何部件，必须将部件装入密封的塑料袋内，并说明袋内 的物品无污染，可安全处理。 Beckman Coulter, Inc.

  • Page 15: 本手册中的警告与注意事项

    安全信息 本手册中的警告与注意事项 警告：如果以 Beckman Coulter,Inc. 未说明的方式使用器械，器械提供的保 护可能受到损害。 警告：小心：请只使用摇动珀耳帖装置附带的缆线。其他缆线可能带来供电或 通信问题。 警告：小心：请勿过度上紧螺丝。摇动珀耳帖装置和配接板的舌片之间有一定 空隙。过度上紧可能导致配接板不平和影响摇动珀耳帖装置的加热与冷却性 能。 警告：警告：触电危险！检查和更换冷却液时需要取下摇动珀耳帖装置的盖板 ；这将暴露内部电气部件。请小心取下盖板，谨慎维修本装置。 Shaking Peltier Device Integration Manual...

  • Page 17: Table Of Contents

    xvii Table of Contents Warranty and Returned Goods Requirements ........iii Safety Information .
  • Page 18 1.2.1 Raising the Biomek 3000 Deck ........

  • Page 19: List Of Figures

    Figure 1-1. Shaking Peltier Device....................1-1 Figure 1-2. Installing new feet on Biomek 3000 instrument ............1-4 Figure 1-3. Attaching the mounting bracket to the Biomek 3000 ..........1-5 Figure 1-4. Adjusting the feet on the mounting bracket .............. 1-6 Figure 1-5.

  • Page 21: Shaking Peltier Device

    Shaking Peltier Device Overview The Shaking Peltier Device (Figure 1-1) is a labware positioner with microplate shaking and heating and cooling capabilities from a connected controller. Each Peltier may be equipped with an adapter plate which allows for different labware types to be used with the Peltier device.

  • Page 22: Control Modes

    3000 in Biomek Software. Refer to the original manuals from the device manufacturer, Inheco, for more information not included in this manual: • Thermoshake Heating and Cooling Unit Manual • TEC Control 96 User’s Manual • Biomek 3000 User’s Manual • Biomek Software User’s Manual Beckman Coulter, Inc.

  • Page 23: Installing The Shaking Peltier Device

    The Shaking Peltier Device is installed on a special mounting bracket that is installed on the left side of the Biomek 3000 deck in place of the side module. The Shaking Peltier Device may be installed on either of two positions on the mounting bracket.

  • Page 24: Raising The Biomek 3000 Deck

    1. Tilt the Biomek 3000 instrument on its back feet. 2. While one person holds the Biomek 3000, a second person screws a new foot into each of the threaded holes adjacent to the two front feet (Figure 1-2).

  • Page 25: Installing The Mounting Bracket

    To mount the mounting bracket on the left side of the instrument: 1. If the Biomek 3000 Side Module is installed on the left side of the Biomek 3000 instrument, remove it (refer to the Biomek® 3000 Laboratory Automation Workstation User’s Manual, Section 1.3.4.1, Removing Installed Side Modules).

  • Page 26: Leveling The Biomek 3000 And Mounting Bracket

    Figure 1-4. Adjusting the feet on the mounting bracket 2. Using a bubble level, adjust the feet on the Biomek 3000 instrument to make sure the deck is level. Turn the feet to the left to raise the feet or to the right to lower them.

  • Page 27: Installing The Shaking Peltier Device

    Installing the Shaking Peltier Device The mounting bracket has two positions in which devices or positioners may be placed. Either a Static Peltier Device, Shaking Peltier Device, or Biomek 3000 Position Holder, in any combination, may be installed in these two positions.

  • Page 28: Figure 1-6. Connections On Watlow Controller For Shaking Peltier Device

    7. Connect the power cable from the main power port on the back of the Watlow controller to a power supply (Figure 1-6). 8. Power on the Watlow controller using the power switch on the back of the Watlow controller just above the power cable (Figure 1-6). Beckman Coulter, Inc.

  • Page 29: Installing Adapter Plates

    Shaking Peltier Device 1.2.5 Installing Adapter Plates Before using the Shaking Peltier Device, one of three available adapter plates must be installed. Adapter plates should be chosen based on what type of microplates are used on the Shaking Peltier Device. Each adapter plate provides a different heating and cooling profile that is optimized for specific types of plates or applications.

  • Page 30: Adjusting The Plate Holder

    2. Using a 1.5 mm Allen wrench, adjust the corner guide screws to control how tightly labware is held in place. The microplate should fit snugly in the position but still move freely in and out of the plate holder. Beckman Coulter, Inc.

  • Page 31: Setting Up The Shaking Peltier Device In Biomek Software

    Setting Up the Shaking Peltier Device in Biomek Software After physically installing the Shaking Peltier Device on the Biomek 3000 Laboratory Automation Workstation, it is must be set up to use in Biomek Software. Setting up the Shaking Peltier Device in Biomek Software includes: •...

  • Page 32: Figure 1-9. Shaking Peltier Silas Module Install

    7. Select Simulation only if using the software on a computer that is not attached to an instrument. This allows methods to be created without the controller attempting to communicate with the device. 8. Choose Next to install the Shaking Peltier SILAS Module. The installation begins. Beckman Coulter, Inc.

  • Page 33: Figure 1-10. Installation Of Shaking Peltier Silas Module Complete

    Shaking Peltier Device 9. When the installation completes, Figure 1-10 appears. Figure 1-10. Installation of Shaking Peltier SILAS Module complete 10. Choose Finish to close the installation program. The Shaking Peltier SILAS Module has been installed successfully. Shaking Peltier Device Integration Manual...

  • Page 34: Adding The Shaking Peltier Device To The Deck In The Deck Editor

    Adding the Shaking Peltier Device to the Deck in the Deck Editor After physically installing the Shaking Peltier Device on the deck of the Biomek 3000 instrument, it must be added to the deck in the Deck Editor. To add the Shaking Peltier Device to the Deck Editor: 1.

  • Page 35: Figure 1-12. Position Properties For Shaking Peltier Device - Expanded

    Shaking Peltier Device 6. Choose More to expand Position Properties (Figure 1-12). Figure 1-12. Position Properties for Shaking Peltier Device — expanded 7. In Device, select the ShakingPeltier device. 8. Choose Manual Teach to frame the position (refer to Section 1.4, Framing Instructions).

  • Page 36: Framing Instructions

    The Shaking Peltier Device must be framed before using it in a method. Framing allows the Biomek 3000 to accurately move labware to and from the Shaking Peltier Device and pipette to and from microplates positioned on the Shaking Peltier Device.

  • Page 37: Attaching The Manual Framing Tool

    (Figure 1-13). Note: Make sure that the arrow marked FRONT on the framing tool is pointing towards the front of the Biomek 3000 deck. 3. Release the button when the framing tool is properly seated. Shaking Peltier Device Integration Manual...

  • Page 38: Manually Framing The Shaking Peltier Position

    Manual Teach is a wizard-type interface that is used to manually frame deck positions on the Biomek 3000 workstation. Using Manual Teach helps to ensure that the tips can access the wells without causing any damage to the tips, tools, or labware positioners.

  • Page 39: Figure 1-15. Position Properties For A Shaking Peltier Device

    Figure 1-15. Position Properties for a Shaking Peltier Device 4. In Name, verify that the position is assigned a unique name. 5. Choose Manual Teach. Biomek 3000 Manual Teaching opens with a Warning (Figure 1-16). Note: On the left side of Manual Teaching, a list of steps required to complete the teaching process is displayed.

  • Page 40: Figure 1-17. Manual Teaching If Framing Adapter Is Not Required

    7. Choose Next to continue to the next screen. Biomek 3000 Manual Teaching displays Load Tool. Figure 1-18. Loading tool during manual teach 8. If not already installed, install the framing tool on the Biomek 3000 head assembly (refer to Section 1.4.1, Attaching the Manual Framing Tool).

  • Page 41: Figure 1-19. Selecting Target To Manual Teach Position

    (from step 9). This selection must match the tip type physically loaded on the framing tool. 11. Choose Next to continue to the next screen. Biomek 3000 Manual Teaching displays Select Target (Figure 1-19). Figure 1-19. Selecting target to manual teach position 12.

  • Page 42: Figure 1-20. Framing To The Center Of A Well

    Z-axis until it is approximately 1 mm above the top of the microplate. Note: Since tip height is set in the next step in the Biomek 3000 Manual Teaching process, it is safe to move the head assembly to any height to make aligning the tips with the target position easier.

  • Page 43: Using The Graphic Alignment Tool

    Shaking Peltier Device 1.4.2.1 Using the Graphic Alignment Tool The graphic alignment tool (Figure 1-21) is used to instruct the Biomek Software of the current position of the framing tool tip to the center of well A1 on the microplate. The Biomek Software uses the information it is given to move the head assembly so the framing tool tip is directly above the center of the well.

  • Page 44: Using The Delta Value And Directional Buttons

    Using the Delta Value and Directional Buttons). 4. Once the tip is aligned with the center of well A1 on the microplate, choose Next to continue. Biomek 3000 Manual Teaching displays Teach Z (refer to Section 1.4.2.3, Framing the Z-Axis).
  • Page 45 (refer to Section 1.4.2.1, Using the Graphic Alignment Tool). 4. Once the tip is aligned with the target position on the microplate, choose Next to continue. Biomek 3000 Manual Teaching displays Teach Z (refer to Section 1.4.2.3, Framing the Z-Axis).

  • Page 46: Framing The Z-Axis

    The directional buttons displayed in Manual Teach parallel the keys on the numeric keypad. More specifically, Fwd. correlates to the ‘1’ on the numeric keypad, while Down is found on the ‘2’, Left is found on the ‘4’, Right on ‘6’, Up on ‘8’, and Back on ‘9’. Beckman Coulter, Inc.
  • Page 47 Shaking Peltier Device 3. Once the framing tool tip is at the same height as the top of the well, select Finish. The pod moves up to its maximum height in the Z-axis, Manual Teaching closes, and Position Properties appears (Figure 1-15). 4.

  • Page 48: Calibrating The Peltier Device Temperature

    8. Press the green button to enter the User menu. 9. Use the arrow keys to scroll to CAL1. 10. Enter the calibration offset value CAL determined in step 5 above. ∞ 11. Press the (infinity symbol) button to set the calibration offset. Beckman Coulter, Inc.

  • Page 49: Using The Shaking Peltier Device In A Method

    Shaking Peltier Device Using the Shaking Peltier Device in a Method Using the Shaking Peltier Device in a Biomek method requires using the Peltier Step step that is displayed on the Peltier step palette (Figure 1-24). The Move Labware step from the Basic Step Palette is used to move labware to and from the Shaking Peltier Device (refer to the Biomek Software User’s Manual, Section 16.4, Move Labware Step).

  • Page 50: Using The Peltier Step In A Biomek Method

    Note: The incubation timer starts when the step is executed, even if it is not at the specified temperature. 5. For Incubate, to set a specified temperature, ensure Set Temperature to is checked and enter a temperature in degrees Celsius (4.0 to 50.0). Beckman Coulter, Inc.
  • Page 51 Shaking Peltier Device 6. For Incubate, select the Shake checkbox to shake the microplate during incubation. 7. For Incubate or Start Shaking, in Shake at, enter the rate of shaking in rotations per minute (RPM). 8. For Incubate, select Continue Shaking after incubation until device receives Open command to continue shaking the microplate after the incubation is complete until the position is accessed for gripping or pipetting.

  • Page 52: Manually Controlling The Shaking Peltier Device

    4. In Communications Port, select the comm port on the Biomek Controller PC to which the Shaking Peltier Device is connected. 5. Choose OK to save the changes and close ShakingPeltier Options, or Cancel to close ShakingPeltier Options without saving changes. Beckman Coulter, Inc.

  • Page 53: Performing Actions In The Device Editor

    Shaking Peltier Device 1.7.2 Performing Actions in the Device Editor To manually control a Peltier action outside a method: 1. From the Instrument menu, choose Device Editor. Device Editor appears. 2. In Device, choose ShakingPeltier. 3. Choose Action Commands. ShakingPeltier appears (Figure 1-28). Figure 1-28.
  • Page 54 This shake sequece repeats until the incubation is complete, the position is accessed, or a Stop Shaking command is sent. 14. Choose Run Now to perform the configured Action. 15. Select Update after completing the action configuration. The ShakingPeltier module action configuration closes. Beckman Coulter, Inc.

  • Page 55: Preventive Maintenance

    Shaking Peltier Device Preventive Maintenance WARNING: ELECTRICAL SHOCK HAZARD! Checking and changing the coolant requires removing the covers from the Shaking Peltier Device, exposing internal electrical components. Remove covers with care and use caution when servicing the device. Check the coolant fluid level every seven weeks and refill as necessary. Refer to the Thermoshake Heating and Cooling Unit Manual for specific instructions.

  • Page 56: Troubleshooting

    Refer to the Thermoshake Heating and Cooling Unit Manual and the TEC Control 96 User’s Manual for additional troubleshooting information. Do not attempt to repair the unit without first contacting a Beckman Coulter Service Engineer. Table 1-1. Troubleshooting the Shaking Peltier Device...

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