First Ten Angstroms FTA125 Quick Start

Kloehn pump

FTA125 and FTA188 Kloehn Pump Quick Start

Last updated October 19, 2005

The pump is shipped with the glass syringe installed. It should not be operated if the syringe

appears damaged. A complete description of the pump, including how to change the syringe, is

contained in Versa3.pdf included with your documentation and available on the FTA website in

the Papers section.

The Koehn pump requires a separate serial port. If your computer lacks enough ports, they can

be easily added with PCI cards or inexpensive USB to RS232 converters.

Initial Startup

You will get a warning message when you click OK on the LogOn screen if the computer can

not establish communication with the pump.

Certain operating parameters of the pumps and motion axes can be changed by the user. This

provides flexibility in running the instrument, but also is an opportunity for mischief. The

numbers must be reasonable. Numbers which appear in blue are for information only – they may

not be directly changed by the user.

"Total syringe capacity": This must match the capacity of the syringe to be driven by a

motorized pump. You can change syringe sizes to scale the pump for very fast or slow

operation. The value is in microliters.

"Automatic rate": This is the rate in microliters per second used in constant-rate dispenses

which are run as part of capturing a Movie. The rate for manual "jogging" is separate, as is the

rate used when the Pump Program is used. Rates which are too fast, typically above 50µl/s, may

465 Dinwiddie Street • Portsmouth, Virginia 23704 • 1.757.393.1584 Fax: 1.757.393.3708

http://www.firsttenangstroms.com

Table of Contents

Do you have a question about the FTA125 and is the answer not in the manual?

Page 1: Initial Startup
FTA125 and FTA188 Kloehn Pump Quick Start Last updated October 19, 2005 Unpacking The pump is shipped with the glass syringe installed. It should not be operated if the syringe appears damaged. A complete description of the pump, including how to change the syringe, is contained in Versa3.pdf included with your documentation and available on the FTA website in...
Page 2 result in motor stalling or loss of steps. The Kloehn pumps have 12000 steps full scale. The slowest rate is one step per minute. “Manual rate”: The rate used for user on/off control when clicking Pump In and Pump Out on the Live screen.
Page 3 Start Auto means to start an automatic pumping operation, just as you would expect if you had checked Start on Run. You can also Run Pump Program now. Pump Tubing The Kloehn pump is shipped with 1/16” (1.6mm) push-on tubing barbs on both the Kloehn pump ports and the needle unions.
Page 4 20 Milliliter Bottle Tray Standard 20cc bottles are provided to hold source liquids. A tray is provided in the accessories to hold these. File: FTA100KloehnPumpQuickStart.doc 465 Dinwiddie Street • Portsmouth, Virginia 23704 • 1.757.393.1584 Fax: 1.757.393.3708 http://www.firsttenangstroms.com...

Fta188