BD BBL RODAC Plate Intended Use

BBL™ RODAC™ Plate
B
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INTENDED USE
BD BBL™ RODAC™ (Replicate Organism Detection and Counting) plates may be used for the
detection and enumeration of microorganisms present on surfaces of sanitary importance.
BD BBL RODAC plates are recommended for use in a wide variety of surface sampling
programs and may be employed to establish and monitor cleaning techniques and schedules.

PRINCIPLES OF HANDLING

The design of the dish permits the pouring of a raised convex surface of the culture medium
for total surface contact of the area being sampled. Culture media recommended for use are
general purpose media containing neutralizers reported to "inactivate" residual disinfectants
where the sample is being collected.
Collection of samples from identical areas "before and after" treatment with disinfectant yields
data useful in evaluating cleaning procedures in environmental sanitation.

PRODUCT DESCRIPTION

The 65 x 15 mm style dish is specially designed to allow a raised convex surface of culture
medium. The 10 mm grid on the bottom of the plate facilitates counting and colony location.
Precautions: For Laboratory Use.
Observe aseptic techniques and established precautions against microbiological hazards
through out all procedures.
Directions for use should be read and followed carefully.

PROCEDURE

Material Provided: BD BBL RODAC Plate, 20 per sleeve, 500 per case.
Materials Required But Not Provided: Bacteriological culture medium with neutralizers,
xylene-based felt-tip marker, wax pencil or labels, incubator (35 °C).
Instructions:
1. Do not use unless immediate package is intact.
2. Select and prepare medium to be used. The use of dehydrated BD BBL D/E Neutralizing
Agar containing five neutralizers (Cat. No. 299038) or BD BBL Trypticase™ Soy
Agar with Lecithin and Polysorbate 80 (Cat. No. 211764) is recommended. For ease in
dispensing, prepared bottled medium may be used; e.g., BD BBL Trypticase Soy Agar
with Lecithin and Poly sorbate 80 (Cat. No. 299623, 500 mL).
3. Dispense 11.0–12.0 mL of the selected medium at about 48–50 °C into each plate on a
level surface. (Figure 1, refer to last page.) Extreme care should be taken to prevent the
formation of air bubbles and to keep the medium from overflowing. If either occurs, the
plates should be discarded. The plates are then allowed to solidify.
4. Use a xylene-base felt-tip marker, wax pencil or label to consecutively number the plates
that are to be used.
5. Note on the report form the location of the site to be tested. Remove the lid and hold it
to avoid accidental contamination. Apply the plate's agar surface directly to the surface
being tested and exert moderate vertical pressure. (Figure 2, refer to last page.) Replace
the cover and repeat with additional plates as required for the sampling program.
NOTE: Caution should be exercised to avoid rubbing on the site; otherwise, the agar bed
may be broken and the usefulness of the plate affected.
6. After samples have been collected, incubate all plates for 48 h at 35 °C.
7. When incubation has been completed, count all colonies. An automatic colony counter
is recommended, or the grid on the bottom of the BD BBL RODAC plate will serve as a
useful guide for estimation.
8. The primary purpose of the BD BBL RODAC plate is to monitor surface cleanliness,
for which a general purpose medium with neutralizers should be used. Isolation and
identification of a specific organism or group of organisms should be done by replicating
or subculturing from the BD BBL RODAC plate to a selective or differential medium for
enumeration. Accurate enumeration of environmentally stressed enteric bacteria from
air and surface samples is often hampered when selective media are employed.
guidance in the interpretation of results, consult appropriate references.

AVAILABILITY

Cat. No. Description
210340 BD BBL™ RODAC™ plate, 65 x 15 mm Style, with 10 mm Grid, 500/case.

References

1. Hall, L.B., and M.J. Harnett, 1964. Public Health Rep. 79:1021.
2. Marshall, R.T. (ed.), 1992. Standard Methods for the Examination of Dairy Products,
16th ed. American Public Health Association, Washington, D.C.
3. Lennette, E.H., et al. (ed.), 1985. Manual of Clinical Microbiology, 4th ed. American Society
for Microbiology, Washington, D.C.
4. Vesley, M.S., and G.S. Michaelsen, 1964. Health Lab. Sci. 1:107.
5. Pryor, A.K., and C.R. McDuff, 1969, Exec. Housekeeper, March.
6. Quisno, R., I.W. Gibby, and M.J. Foter, 1946. Am. J. Pharm. 118:320.
7. Erlandson, A.L., Jr., and C.A. Lawrence, 1953. Science 118:274.
8. Brummer, B. 1976. Appl. Environ. Microbiol. 32:80.
9. Baron, E.J., L.R. Peterson, and S.M. Finegold, 1994. Bailey and Scott's Diagnostic Micro-
biology, 9th ed. Mosby-Year Book, Inc., St. Louis.
10. Murray, P.R., et al. (ed.), 1995. Manual of Clinical Microbiology, 6th ed. American Society
for Microbiology, Washington, D.C.
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