Pop-Bio VU-C User Manual

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Vü Imaging Systems

User Manual

VÜ IMAGING MANUAL V2.0

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Page 1 Vü Imaging Systems User Manual VÜ IMAGING MANUAL V2.0...
Page 2 The contents of this manual have been checked for accuracy and every effort has been made to ensure there are no errors. Pop-Bio Ltd makes no guarantee as to the accuracy of this document and reserves the right to make changes to the contents without prior notice.
Page 3: Safety Precautions
This symbol indicates WARNING Not following instructions could lead to serious injury or death to users. Power Supply Use only the power supply provided by Pop-Bio Ensure the mains power to all devices are switched off before connecting or disconnecting cables Ensure mains plug is fully inserted into socket to avoid ‘arching’...
Page 4 Use only parts supplied by Pop-Bio Imaging Only suitably qualified persons should conduct any maintenance or service operation Illumination The Blue LED light present a low risk and in normal operation should not be visible to the user The UV tube does present a UV hazard however in normal operation should not be visible...
Page 5: Table Of Contents
Contents Page 3 Safety Precautions Page 6 The Vü Imaging system Page 7 System requirments Page 8 Getting to know your system Page 9 Setting up your system Page 10 Installation and installing the Vü software Page 13 Using the Vü-F Page 16 Using the Vü-C Page 19...
Page 6: The Vü Imaging System
The Vü Imaging system The Vü imaging system is available in two configurations. Vü-C is for chemiluminescence applications. The unit has built in RGB LED’s which are used for illumination of marker lanes on a blot. No other lighting is available. The Vü-C is used exclusively for chemiluminescent western blot applications Vü-F is for Fluorescence application.
Page 7: System Requirments
System requirements The Vü system can be operated as follows: The Vü system can be connected to a network either by ethernet cable or a WiFi adaptor. There is an ethernet port on the rear of the system and spare Wifi slots for a WiFi adaptor. It is also possible to connect the system directly to a computer using the ethernet cable.
Page 8: Getting To Know Your System
Getting to know your system Front View Rear View VÜ IMAGING MANUAL V2.0...
Page 9 Check for any damage to the unit or packing. Report any damage in the first instance to the shipper and then to Pop-Bio for further instruction or advice. Retain all packing material if the unit needs to be shipped later.
Page 10 Installing the unit Place the unit on a clean, flat, dry surface with the front drawer being easy to reach Locate the 4 pin socket on the rear of the unit and plug the lead from the power supply into this. Using the power cable supplied connect the power supply to a suitable mains outlet.
Page 11 [if a Vu-F and a Vu-C are being used] being connected to the facility network using either an ethernet or Wifi connection method. For all connections you will need to use a Network name. This name is assigned to the unit during the manufacturing process.
Page 12 Click here to find Version Number 5. To check the version number you have just loaded on your computer click the Popbio Imaging logo on the top left hand corner of the screen and see the ‘About Vu’ option. Click this to see the version number of your software.
Page 13: Using The Vü-F
Using the Vu-F Imaging system • Open the Vu software Select the system you want to use by clicking on the system icon. Select Vü-F [ Blue] Plug out the drawer from the front of the unit as far as you can. It will reach a ‘stop’ point. Remove the tray insert from the drawer.
Page 14 • User [ numbers 0-11]. If you are not using user ID then this should be set to position 0. [See more about User ID later] 4. Push the drawer in all the way. The system will start automatically The system will now begin its process and will typically take around 20 -90 seconds to complete the acquisition and processing.
Page 15 Hold this button in for 2-3 seconds You may hear the sensor head moving to its park position. Once parked the indicator light glows solid red. In this position the unit can be switched off from the mains and safely moved to another location. Switching the unit back on moves the sensor head out of the park position.
Page 16: Using The Vü-C
Using the Vü-C Imaging system Open the Vu software Select the system you want to use by clicking on the system icon. Select Vü-C [ Yellow] Plug out the drawer from the front of the unit as far as you can. It will reach a ‘stop’ point. Remove the tray insert from the drawer.
Page 17 • User [ numbers 0-11]. If you are not using user ID the setting should be 0. [See more about User ID later] 4. Push the drawer in all the way. The system will start automatically The system will now begin its process and will typically take around 2 -5 minutes to complete the acquisition. The image will appear on the computer screen in the Vü...
Page 18 You may hear the sensor head moving to its park position. Once parked the indicator light glows solid red. In this position the unit can be switched off from the mains and safely moved to another location. Switching the unit back on moves the sensor head out of the park position. VÜ...
Page 19: How To Get The Best Images From Your Vü System
If the bands are too faint then increase the exposure time. If they are too bright then decrease the exposure time. To do this click onto the Pop-Bio Imaging logo on the top left-hand side of the screen and a menu appear.
Page 20 From this menu select ‘Manual Scan’ and a set-up box appears like this- Here you can see which illumination type you have just used [ UV, converted, chemiluminescence or Blue (as shown in this case) and the exposure time. So, if the image you have just captured using blue light is too faint –...
Page 21 When you click Optimize the software analyses the image you have just captured. It then measures the signal levels. It will then automatically change the exposure setting [ either up or down] to what it thinks will give you the best image based on the signal it sees. Once you have the Optimize image it is a good idea to note the exposure time used [ use the ‘click for more’...
Page 22 All the grey levels have been used and the image ‘stretched’ to do this. The result can sometimes seem to reduce the brightness of the bands at the expense of other not so important parts of the image. The simple trick here is to zoom into the image to focus on the areas of the gel or blot which you think are important.
Page 23 Using the image enhancement functions- manually Instead of using the ‘Auto’ box to make the image look good you can do all the above yourself using the sliders below the image. To use these, un-tick the ‘Auto’ box. Now find the sliders to the right-hand side of this.
Page 24: Understanding The Vü Software
Understanding the Vü Software The Vü systems are built in such a way that the user has the need to control very little. All of the image acquisition and processing is automatic with the intelligent software making the decisions depending on the application/lighting selected by the user.
Page 25 Image display Zoom To increase or decrease zoom: roll the mouse-wheel forwards or backwards. On a laptop, the touchpad may offer different functionality depending on the laptop model and settings: • the “pinch” gesture: touch the pad with two fingers then open or close fingers to control zoom •...
Page 26 Modify user settings If the user (number selected on scanner tray thumbwheel) has not previously set up using the User Settings (see section 0 below), a message box is presented when the scanner sends an image. Click Yes button to change settings for this user: •...
Page 27 Users On the main window, click the Users button to present the User Settings dialog: User List A list of users corresponds to numbered positions on the tray thumbwheel. Change Click the Change button for any user to change automatic behaviour on each scan. A menu is presented: Automatically Save All Scans to Folder...
Page 28 Clear settings for this user The user name and save action will be cleared for this entry on the list, cancelling any automatic save actions for this user. User 0 The settings for User 0 (number zero) are often used as a “default” or “catch all” if lab users have not yet set up their own save locations.
Page 29 Click here of additional Menu Here you will find • Manual Scan • Network setting • About Vü VÜ IMAGING MANUAL V2.0...
Page 30 Unless you have changed the hostname (see below), it will match the serial number of the system. Vu-F Serial number format is vu-fluor-04xxxxxx The number if found on the rear of the unit Vu-C Serial number format is vu-chemi-03xxxxxx The number is found on the rear of the unit. VÜ IMAGING MANUAL V2.0...
Page 31 If the scanner cannot be located on your network, Trying to connect appears next to the hostname. As you enter the name of your Vü scanner, the connection is retested continuously. When the scanner is located on your network, Connected appears next to the hostname.
Page 32 Scanner WiFi Check the Enable WiFi on Scanner checkbox to access the WiFi controls: If you have plugged a supported WiFi adaptor into your Vü Scanner, these controls allow you to link it to your wireless network. You must connect to the Vü Scanner using an Ethernet cable before changing these settings, and it is recommended that you consult your Network Administrator before doing so.
Page 33 Manual Scan Here you can enter in a new expsoure time to change the one that is preset by the lighting thumbwheel. Simply chose the lighting type you wish to change and type in the new exposure time in the box. Note this setting does not change the permanent expsoure time setting for the thumbwheel.
Page 34: Taking Care Of Your Vü
Taking care of your Vü Keep your Vü clean to ensure optimum performance Cleaning the gel/blot tray Remove the tray insert and wash under running water after use. This will avoid long term build-up of dried liquids on the surface of the glass. Dry using a non-abrasive cloth or a paper towel.
Page 35: Maintenance And Settings
Maintenance and Settings The Vü has very few parts that require servicing. In the unlikely event of a problem then only suitably qualified service personnel should attempt to remove the cover of the unit. The only routine maintenance that can be undertaken by the user is to change the UV tube in the Vü-F system Changing the tube Ensure the Vü-F has been set to ‘park’...
Page 36 Commission – Service Engineer only This dialog box is reserved for the use of a trained Service Engineer only. Please see separate service instructions for the use of the options in the box Configure Thumbwheels – Service Engineer only Calibration •...
Page 37 Testing • Start with the drawer open • On the Commissioning dialog, press Test thumbs • Repeat for all positions of each thumbwheel: o Set the thumbwheels, close the drawer, open o Check the reported values • Close the tray and leave it closed so that the command terminates If you see any failures, please save the image that has built up on the image view and send us the file.
Page 38 Information for Vü networking connections and configurations The following tells you how you can connect to your Vü scanner. It cannot teach you all about computer networks, so you may need to seek further information, advice or guidance from elsewhere. Your IT staff may also wish to be involved in configuring the networking options of your scanner.
Page 39 DNS also requires a central service on your network (your router or other server) to provide a centralised way to map the names to IP addresses. (DNS also enables you to type “www.google.com” instead of requiring you to know the IP address of Google’s server.) You can connect to your scanner using its IP address instead of its hostname –...
Page 40 Networked configuration If you have a ‘proper’ network in your office or laboratory that provides shared network storage, connection to the internet, shared printers etc. then it’s much simpler to just connect the Vü scanner to the network. The network will provide DHCP and DNS, and you can refer to the scanner by name without any difficulty.
Page 41: Specifications
Specifications Vü-F Vü-C Application Fluorescent gels and blots Chemiluminescence Western blots Illumination RGB integral LEDs for marker including coloured markers UV integral 302nm [1 tube] Blue light integral LEDs Blue Green LEDs [optional] White light conversion screen Sample size (cm) 20 x 20 10 x 10 Sample Drawer...
Page 42 Pop-Bio Imaging Milton Hall Ely Road Milton Cambridge, UK CB24 6WZ Tel: +44 (0) 1223 620020 Email: sayhello@pop-bioimaging.com Website: www.pop-bioimaging.com VÜ IMAGING MANUAL V2.0...

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