Summary of Contents for Laxco, Inc. Alpha-1000
- Page 1 Alpha-1000 SPECTROPHOTOMETER USER’S MANUAL V 1.0 Revision 0 Laxco, Inc. http://www.laxcoinc.com...
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Page 3: Table Of Contents
Table of Contents Introduction ....................... 4 Working Principle ...................... 4 Specifications ......................5 Unpacking Instructions ....................6 Installation ......................... 6 Operation Panel ......................6 Changing Sample Holders ..................8 Basic Operation ......................9 Absorbance and Transmittance Measurement ..........9 Making Measurements at Different Wavelengths .......... 10 Lamp Changing ....................... -
Page 4: Introduction
Introduction The Alpha-1000 is a single beam spectrophotometer, which is designed to meet the needs of both students and instructors. Its digital display, easy operation, and wavelength range of 400 nm to 1000 nm makes this unit ideal for spectrophotometric experiments in the visible wavelength region of the electromagnetic spectrum. -
Page 5: Specifications
Specifications Table-1 lists the specification for the Alpha-1000. Table-1 Wavelength Range 1000 nm Spectral Slit Width 15 nm Wavelength Accuracy ± 3 nm Wavelength Readability 1 nm ≤ 2%T at 400 nm Stray Radiant Energy Photometric Range 0%T to 125%T, -0.3A to 2.0A, Photometric Accuracy Better than ±... -
Page 6: Unpacking Instructions
3. Turn on the instrument and allow it to warm up for 15 minutes before taking any readings. Operation Panel The key operating components of the Alpha-1000 spectrophotometers are shown in Figure-2 & Figure-3. The power switch is located on the back of the instrument. Power Switch: Turns the instrument on and off. - Page 7 Wavelength Readout Window: Displays desired wavelength. USB port: It is used for connecting to Computer with Application Software RS232: It is used for Firmware upgrading. Digital Display Sample Compartment Operation Instructions Wavelength Control Knob 0A/100%T Knob Wavelength Readout Window Figure-2 Alpha-1000 Spectrophotometer...
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Page 8: Changing Sample Holders
Back view of Alpha-1000 Changing Sample Holders Your Alpha-1000 comes standard with the A-110-102 Sample Holder. We offer three additional holders as optional accessories. Please refer to Table-3 “Alpha-1 Series Parts List” in Alpha-1 Series Parts List of this manual for details. -
Page 9: Basic Operation
Lock Screw Figure-4 Changing Sample Holders Figure-5 Allen Wrench Basic Operation Simple OPERATION INSTRUCTIONS are printed on the front panel of your Alpha- 1000. Absorbance and Transmittance Measurement Making a measurement of absorbance and transmittance is a two-step process. In the first step, a test tube filled with a transparent solution is placed into the sample compartment. -
Page 10: Making Measurements At Different Wavelengths
The step-by-step procedure for making an absorption/transmittance measurement is as follows: 1. Turn on the spectrophotometer by pressing the power switch (the power switch is located on the right side of the back of the instrument.) In order to stabilize the lamp and detector;... -
Page 11: Lamp Changing
Secure the bulb with the metal bracket. Use replacement bulbs with an Item Number of A-110-505. 5. Replace the lamp-housing panel. Secure the screw. Bottom View Lamp house Screw Spill release stopper Figure-6 Bottom View of the Alpha-1000... -
Page 12: Lamp Adjustment
Bulb can be VERY HOT Figure-7 Bulb View of the Alpha-1000 Lamp Adjustment The position of your lamp has been factory adjusted to its optimal setting. Follow these steps below for adjusting the lamp position. These steps should NOT be needed! Unfortunately, one should COMPLETELY close the light chamber between adjustment settings attempts. -
Page 13: Absorbance Accuracy And Stray Light Checking
4. Once the optimum position has been obtained, verify that the lamp chamber door is secure and all items are returned to their original state except the lamp bracket position. Absorbance Accuracy and Stray Light Checking Specification: ± 2% at 1A (100DPlus), ± 1% at 1A and 2A. The absorbance accuracy and Stray Light should be checked against a set of neutral density filters accurately calibrated to the NIST standards. - Page 14 When the transmittance is 100% (T=1), A=log (1) = 0 and increases as the transmittance decreases. The maximum value of the absorbance read by the Alpha-1000 is 2.0. This represents a Transmittance of: T=1/10 or about 1%. This is a very small transmittance and is essentially zero.
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Page 15: Experiment 2 Measurement Of An Absorption Spectrum
Experiment 2 Measurement of an Absorption Spectrum The absorption spectrum of a substance helps identify it. In this experiment, you will measure the absorbencies of Cr (chromium III ions) at a series of wavelengths in the visible light region and draw its absorption spectrum. The materials required for this experiment are 20 ml 0.5M Cr (aq) solution and two round cuvettes. -
Page 16: Experiment 4 Determination Of The Concentration Of A Solution
The materials required for this experiment are 500 ml 0.04 g/l thymol blue solution, 40 ml 1 M HCL (aqueous), a 50 ml burette, a 10 ml graduated cylinder, a 100 ml volumetric flask, five round cuvettes, and four Erlenmeyer flasks. Procedure: Fill the burette to the top calibration line (50 ml) with the thymol blue stock solution. - Page 17 flasks, a 2 ml pipette, a 10 ml pipette, six 25 ml test tubes, six round or square cuvettes, and a hot plate. Procedure: Fill the burette to the top calibration line (50 ml) with the glucose stock solution. Deliver 5 ml of the glucose solution from the burette into the volumetric flask.
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Page 18: Experiment 5 Determination Of A Species In A Solid Sample
axis absorbance and mark it in equal intervals from 0 to a convenient round value above your highest data point. 2. Plot absorbance versus concentration for the four standards. Draw the best straight line through the origin and the four points. 3. - Page 19 Transfer the solution from the beaker to the 100 ml volumetric flask. Dilute the de-ionized water and mix thoroughly. This is your diluted extraction solution. Filter 20 ml of the diluted extraction mixture through filter paper in a gravity funnel into a flask or beaker. Wash the volumetric flask thoroughly with de-ionized water.
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Page 20: Parts List
Parts List Table-3 Alpha-1 Series Parts List Item Number Description A-1000 Model Alpha-1000 Spectrophotometer 15 nm Band pass Wavelength range: 400~1000 nm. Complete with 10 mm Test Tube Cuvettes (12 pcs.) 10 mm Cuvette Adapter, Dust Cover, User Manual Accessories A-100-101... -
Page 21: Troubleshooting
Troubleshooting PROBLEM Possible Cause Solution Instrument Power cord not connected to outlet Plug instrument in Inoperative Internal fuse blown or defective Call an authorized service (Power indicator electronic component engineer has no light) Instrument No Cuvette Adapter in the Sample Cuvette Adapter must be in Sample Compartment to cannot be set to... - Page 22 Possible Cause Solution PROBLEM Check lamp has been Instrument drift No sufficient warm up time and noise Significant temperature change properly installed or has Lamp not adjusted properly moved during transit Refer to Lamp Replacement instructions in this manual Lamp old or defective Replace with a new lamp Sample Holder Misaligned Refer to Lamp Replacement...
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