Related Manuals for Tecella Triton Plus
Summary of Contents for Tecella Triton Plus
- Page 1 3001 Red Hill Ave, Suite 1-204 Costa Mesa, CA 92626 Phone +1-714-641-1709 Fax +1-714-641-1569 Triton+ User’s Manual...
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Page 2: Table Of Contents
Convert Data to Tab Delimited or ATF Format ........22 3.10 Using TecellaLab ..................22 Getting Started With WinWCP ................24 Download WinWCP Setup File ..............24 Download WinWCP User Guide............24 Install WinWCP ..................24 Start WinWCP..................24 Set Laboratory Interface Card to “Tecella Triton”........25 Using WinWCP ..................26 Connecting To Your Setup..................27... - Page 3 Using TecellaLab....................28 Hardware Calibration.....................29 Check For Hardware Drift...............29 Option 1: Re-start the Application ............29 Option 2: Re-run Hardware Calibration ..........30 Specifications......................32 Source Selector..................32 Headstage and Feedback Gain Resistor (Rf) ........32 Current Range & Current Resolution.............33 A/D Resolution & Sampling Rate ............33 Analog Filter ....................33 Noise......................34 Capacitance Compensation ..............35...
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Page 4: Before You Begin
Chapter Before You Begin Introduction The Triton+ is a full-featured whole cell patch clamp amplifier, available in 1, 2, 4, or 8 channel configurations. The digitizer, the head stages, and the model cells are all integrated inside the Triton+ amplifier simplifying setup of the work environment. Triton+ communicates to the computer via a standard USB 2.0 cable, so no special hardware is required on the computer. -
Page 5: Installation
Chapter Installation Select the Driver Setup File for Your OS Before plugging in the amplifier to the computer for the first time, please follow the following steps to install the FrontPanel driver file onto your computer. The FrontPanel driver file comes in 32-bit version and 64-bit version. -
Page 6: Install Driver File Onto Your Computer
Install Driver File Onto Your Computer Follow the steps below to install the FrontPanel driver file onto your computer. First, you will see the Setup Wizard welcome screen. Click on “Next” to proceed. Next, you will see Opal Kelly’s License Agreement. Click on “I Agree”... - Page 7 This screen may momentarily appear during driver installation. After the FrontPanel driver files has been successfully installed on your computer, you will see this confirmation screen. Click on “Finish” to exit the installation. Although the driver files have been installed on your computer, they have not been associated with the amplifier yet.
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Page 8: Connect The Power Supply To The Amplifier
Connect the Power Supply To the Amplifier Power Locate the connector of the power supply. Connector Locate the power jack on the back of the amplifier as indicated by the arrow below. Power Jack Insert the power connector firmly into the power jack. -
Page 9: Plug In The Power Supply Into An Ac Outlet
Caution! Extremely Important When Detaching Power Cable When detaching the power cable from the amplifier, in order to disengage the locking mechanism, you must first pull back on the connector cover as shown. As you pull back on the connector cover, the silver colored shield should become visible. -
Page 10: Connect The Usb Cable To The Amplifier And The Computer
Connect the USB Cable To the Amplifier and the Computer A standard Mini-USB cable (as shown) is included with the amplifier. Plug the small end of the USB cable into the USB port of the amplifier indicated below. Plug the large end of the USB cable into the USB port of your computer. The USB port must be USB 2.0. -
Page 11: Associating The Driver And The Amplifier (Windows Xp)
Associating the Driver and the Amplifier (Windows XP) Automatic Association On Windows Vista and Windows 7 Windows Vista and Windows 7 will automatically associate the FrontPanel driver and the amplifier. No further action is required. For Windows XP computers, please proceed with the following steps. The first time you connect the amplifier to your computer, “Found... - Page 12 If this screen pops up, click on “Continue Anyway”. You will see this screen as the amplifier gets installed on your computer.
- Page 13 You will see the following after the amplifier finishes installing. Click “Finish” to exit the wizard. You are now ready to begin using the amplifier. The amplifier is now ready for use.
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Page 14: Getting Started With Tecellalab
Chapter Getting Started With TecellaLab Now that the amplifier has been installed, the following steps will help you get familiar with the amplifier quickly using the TecellaLab software. WinWCP Users If you wish to use the WinWCP software, please jump to the next chapter. Install TecellaLab TecellaLab can be installed on your computer by simply unzipping the software zip file to a desired folder on your computer. - Page 15 We recommend you extract the files to the root folder of your main drive, usually the C:\ folder, because data recordings are saved to a subfolder of the TecellaLab folder by default. Double click on the TecellaLab folder. You will see TecellaLab.exe file which is the TecellaLab software.
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Page 16: Launch Tecellalab
Create a Desktop Icon For ease of access, we recommend you create a desktop icon. To do so, right click on the TecellaLab.exe icon, then choose Send To and then Desktop. Launch TecellaLab Start TecellaLab by double clicking on the Desktop icon you just created. If you did not create a desktop icon, then you can start TecellaLab, by double clicking on the TecellaLab icon in the TecellaLab folder that you created. -
Page 17: Start Acquisition
Start Acquisition Click on the Acquire button in the upper right to begin acquisition. You should see a waveform like this. Double Click in the waveform section to zoom out and fit the waveform. You can now see the entire waveform. Holding down the left mouse button allows you to move the... -
Page 18: Changing Gain (Rf Setting)
Changing Gain (Rf setting) Pull down on the Gain menu and select 1Gig. Notice the substantial reduction in noise at the higher gain. -
Page 19: Changing Source
Changing Source Pull down on the Source menu and select Model. The Triton+ comes equipped with a whole celll model cell on every channel. The model cell consistsw of 10Meg series resistance and 100Meg seal resistance. Notice the current flowing through the model cell. -
Page 20: Save Data To A File
Save Data To a File Warning! Make sure acquisition is stopped before proceeding. If the Acquisition/Stop button is displaying “Stop”, then you are in acquisition mode. Stop the acquisition by pressing on the “Stop” button. When acquisition stopped, the button should display “Acquire”. - Page 21 Click on “Save” to set the output file name and exit the dialog box. Data is saved in TLC (Tecella Lossless Compression) format with file extension of TLC. TLC files can be converted to tab-delimited, ATF format using the tlc2atf.exe utility.
- Page 22 The output file name is displayed in TecellaLab. Acquisition data will now be saved to this file. Click on “Acquire” to begin acquiring data and saving it to the output file. Click on “Stop” to stop acquisition and to write the data to the output file.
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Page 23: Convert Data To Tab Delimited Or Atf Format
Go to the “captures” folder in your TecellaLab folder to access the captured data. Convert Data to Tab Delimited or ATF Format Click on the Start button to begin acquisition. You should see a waveform like this. 3.10 Using TecellaLab For additional detailed information on the use of TecellaLab, please refer to the chapter titled “Using TecellaLab”. -
Page 25: Getting Started With Winwcp
Chapter Getting Started With WinWCP WinWCP is a full featured 3 Party software application that works with Tecella’s Triton+ amplifiers. Download WinWCP Setup File First, go to the following web site. http://spider.science.strath.ac.uk/sipbs/page.php?show=software_ses Download the WinWCP software, by clicking on the link that says: WinWCP Vx.x.x Setup File... -
Page 26: Set Laboratory Interface Card To "Tecella Triton
Set Laboratory Interface Card to “Tecella Triton” Click on “Setup” in the top menu. Select “Recording sweep”. Click on the pull-down menu under “Laboratory Interface Card”. -
Page 27: Using Winwcp
Select “Tecella Triton”. Tecella Triton has been selected. You are now ready to use WinWCP with the Triton+. Using WinWCP For information on how to use WinWCP, please refer to the WinWCP User Guide that you downloaded previously. -
Page 28: Connecting To Your Setup
Chapter Connecting To Your Setup... -
Page 29: Using Tecellalab
Chapter Using TecellaLab... -
Page 30: Hardware Calibration
Chapter Hardware Calibration The hardware is automatically calibrated every time TecellaLab or WinWCP is started. Under normal use, there should not be any need to re-calibrate the hardware. However, if you suspect that the hardware calibration has drifted, due to heat, as an example, the hardware can be recalibrated with TecellaLab or by closing and re-starting your application, either TecellaLab or WinWCP. -
Page 31: Option 2: Re-Run Hardware Calibration
Option 2: Re-run Hardware Calibration Calibrate Internal Offset Click on the “Calibrate” button to compensate for internal amplifier offsets. Notice that baseline offsets have now been removed. - Page 32 Calibrate Scale Click on the “Scale” button properly scale gain resistors. Notice that currents through the model cells have been scaled uniformly across all channels.
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Page 33: Specifications
Chapter Specifications Source Selector The connection to the input of the amplifier is determined by the Source Selector, and the following choices are available. Source Selector also determines whether the attached external object is being clamped by the amplifier. Source Selector can be controlled independently for each channel. Source Connection Is External Object... -
Page 34: Current Range & Current Resolution
Current Range & Current Resolution Values for measurable current range and current resolution are listed in the following table, and are dependent on the selected Feedback Gain Resistor (Rf). Feedback Gain Current Range Current Resolution Resistor (Rf) ±450nA 10Meg Ohm 15.3pA ±45nA 100Meg Ohm... -
Page 35: Noise
Noise Noise level is dependent on the selected Feedback Gain Resistor (Rf), and the values are listed in the following table. Feedback Gain RMS Noise Conditions Resistor (Rf) (typical) 10Meg Ohm 65pA Source: None Filter: 3kHz 100Meg Ohm Vcmd: 1Gig Ohm 0.8pA 5Gig Ohm 0.2pA... -
Page 36: Capacitance Compensation
Rf = 1Gig RMS Noise = 0.8pA Rf = 5Gig RMS Noise = 0.2pA Capacitance Compensation The Triton+ provides 1 Fast Capacitance (Cfast) and 3 Slow Capacitance (Cslow) compensation circuits. Cfast compensation is typically used to compensate capacitive spikes created by capacitance in the cable, chip, and at the input to the headstage. Cslow compensation is typically used to compensate capacitive spikes created by membrane capacitance. -
Page 37: Series Resistance Compensation
Series Resistance Compensation The Triton+ provides Series Resistance compensation circuit on every channel. Parameter Value Range 0 to 20Meg ohm Note: Series Resistance Compensation circuits can be customized prior to purchase or after purchase, for an additional fee. Analog Leak Compensation (optional) The Triton+ provides an optional Analog Leak Compensation circuit. -
Page 38: Junction Offset Compensation
8.12 Junction Offset Compensation Junction Offset specification is listed below. Parameter Value ±255mV Range Resolution 0.1mV Note: Junction Offset circuit can be customized prior to purchase or after purchase, for an additional fee. 8.13 Zap specification is listed below. Parameter Value ±1000mV Range... -
Page 39: Troubleshooting & Support
Chapter Troubleshooting & Support Technical Support Please contact Tecella for technical support at:: Email: support@tecella.com Phone: +1-714-641-1709 Fax: +1-714-641-1569 Mail: Tecella 3001 Red Hill Ave, Suite 1-204 Costa Mesa, CA 92626...
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