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Sutter Instrument Lambda 421 Operation Manual

Ultra-high-speed optical beam combined 4-channel wavelength switching led light source system
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Lambda 421
Ultra-High-Speed Optical Beam Combined
4-Channel Wavelength Switching
LED Light Source System
(With Lambda DG-4 External
Control Compatibility)
Operation Manual
Rev. 1.00n ((20170623)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com

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  • Page 1 Lambda 421 Ultra-High-Speed Optical Beam Combined 4-Channel Wavelength Switching LED Light Source System (With Lambda DG-4 External Control Compatibility) Operation Manual Rev. 1.00n ((20170623) One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 Copyright © 2017 Sutter Instrument Company. All Rights Reserved.

  • Page 5: Disclaimer

    SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the Lambda 421 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument is designed for use in a laboratory environment that has low electrical noise and mechanical vibration. Surge suppression is recommended at all times.

  • Page 6: Avoiding Physical Injury While Powered Up And Emitting Light

    I) that is free from mechanical vibrations, electrical noise and transients. DO NOT CONNECT OR DISCONNECT THE LQUID LIGHT GUIDE BETWEEN THE LAMBDA 421 AND MICROSCOPE ADAPTER (OR THE ADAPTER FROM THE MICROSCOPE) WHILE POWER IS ON. Operate this instrument only according to the instructions included in this manual.

  • Page 7: Table Of Contents

    3.3.5 USB Device Interface Connector....................8 3.3.6 SERIAL Interface Connector....................8 3.3.7 PARALLEL Interface Connector .....................8 3.4 Operation ............................8 3.5 Understanding the Basic Operation of the Lambda 421..............9 3.6 Manual control ..........................9 3.7 Control via the DG-4 Interface......................10 4. MAINTENANCE..........................13 4.1 Routine Maintenance ........................13 5.
  • Page 8 Figure 3-1. Locations of individual indicators, controls, and connectors on the Lambda 421 controller............................ 7 Figure 3-2. Rear panel of the Lambda 421.....................11 Figure 5-1. Power entry module and fuse location on the rear panel of the Lambda 421 controller.21 LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 9: Introduction

    1. INTRODUCTION 1.1 Introduction The Lambda 421 is an illumination system designed for the rapid change of wavelength derived from up to four light sources of different wavelengths combined into a single beam. The system comprises of three subsystems: controller, Optical Beam Combiner (ODC), up to four LED light sources, and power supply.

  • Page 10: General Description

    SELECTION AND BEAM REFLECTION USING SEMROCK-STR FILTERS Figure 1-1. Lambda 421 indicators, controls, and connectors. The Lambda 421 beam combiner is a new, patented, concept for combining separate light sources with different spectra into a single common output beam. Each separate light source is collimated before entering the optical path through a bandpass filter.

  • Page 11: Figure 1-2. Stages Of Pass-Through And Reflection From Each Input Light Source

    Figure 1-2. Stages of pass-through and reflection from each input light source. The Lambda 421 was designed to keep the size of the beam combiner small, and the optical path short and efficient. Thin-film bandpass filters, such as Semrock’s STR, reflect greater than 90% of out-of-band light.
  • Page 12 LEDs. In the case of LEDs, wavelengths can be shuttered at the speed of the individual source. Sutter Instrument Lambda 421, HPX, and TLED products can switch in 10-25 microseconds respectively, making the Lambda 421 one the fastest wavelength switcher on the market.

  • Page 13: Installation

    5. Ensure that that the right side switch for each channel (Channel 0 through 3) is in the OFF position. 6. Power up the Lambda 421 rocking the power switch (far right side of the front panel) upwards in “1” position. A red LED immediately to the left of the power switch will illuminate when power is on.
  • Page 14 LIGHT GUIDE! The output of the light or the light guide should be directed into the microscope using the appropriate adapters, directed away from anyone’s eyes, and not directed toward any reflective surface. LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 15: Operations

    3. OPERATIONS This chapter describes the operation of the Lambda 421. The locations of the individual indicators, controls, and connectors are indicated in the following figure. Figure 3-1. Locations of individual indicators, controls, and connectors on the Lambda 421 controller.

  • Page 16: Indicators

    PARALLEL port of a computer using specialized software. 3.4 Operation The Sutter Optical Beam Combiner (OBC) installed on the Lambda 421 allows for up to four light sources with differing spectral output to be combined and delivered on a common output light path.

  • Page 17: Understanding The Basic Operation Of The Lambda 421

    LED and the operating current for that LED. The new Lambda 421 system combines four of our high power LEDs and their drivers, a control circuit board from the new USB version of the DG-4 and the OBC to form a compact, complete light source that functions like a DG-4 without any moving parts.

  • Page 18: Control Via The Dg-4 Interface

    3.7 Control via the DG-4 Interface In order to understand the Lambda 421 it is important to know the way the DG-4 functions. The DG-4 selects one of 4 filter paths for the light source. The filter values, in the DG-4 context, are actually just memory locations for the settings assigned to a filter value for positioning the two galvanometers in the optical system.

  • Page 19: Figure 3-2. Rear Panel Of The Lambda 421

    LEDs then is entirely from the DG-4 interface. The connections for the DG-4 interface are all available on the back panel. Figure 3-2. Rear panel of the Lambda 421. The USB interface to a control computer is the default method of control. In most cases the USB port will be used in the virtual com port mode which enables this port to support software that was developed for use with serial port control.
  • Page 20 These are programmed as indicated in the previous table. However, each value can easily be changed from external software. The new Lambda 421 has a new series of trigger options for the ring buffer. Triggering on the rising edge or the falling edge of the trigger signal can be selected. A special mode that allows the level of the trigger signal to also control the shutter function is also available.

  • Page 21: Maintenance

    4. MAINTENANCE 4.1 Routine Maintenance Routine cleaning of the Lambda 421 system is required to prevent excessive dust accumulations. Wipe all exterior surfaces with a dry, soft, cotton cloth. Periodically inspect all cables ensuring that all connections are made well and connectors are evenly seated.
  • Page 22 (This page intentionally blank.) LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 23: Changing Led Modules

    1.5mm Allen wrench. It advisable to turn the knob output down to 0. 2. With the knob(s) removed, it should look like the image below. Please note the opening in the sheetmetal: LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 24: Adjustment Procedure For Switching Between A 5A Led To 1A Or 2A

    5. Slowly turn up the knob until you start to see a voltage appear on the Multimeter and the LED producing light output 6. Turn the intensity knob all the way up and you will see a Voltage of .5V for a 5A setting. LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 25: Adjustment Procedure For Switching Between A 1A Or 2A To 5A

    3. Remove and replace the Filter assembly 4. Turn your Multimeter ON and set to Volts (DC) 5. Turn on the Main power of the Lambda 421 6. With the LED connected to channel that you’re adjusting flip both switches in the up position “INT”...
  • Page 26 FG-OBC-561 FG-OBC-590 FG-OBC-630 FG-OBC-660 FG-OBC-740 FG-OBC-850 FG-OBC-940 LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 27: Appendix A. Limited Warranty

    Warranty work will be performed only at the factory. The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer.
  • Page 28 (This page intentionally blank.) LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 29: Appendix B. Fuse Replacement

    Figure 5-1. Power entry module and fuse location on the rear panel of the Lambda 421 controller. The fuse holder holds two fuses. Both fuses are of the same type and rating. If either fuse is blown, it is recommended that both fuses be replaced.
  • Page 30 (This page intentionally blank.) LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 31: Appendix C. Technical Specifications

    Mains fuse (rear of cabinet) Time delay (or time lag) 5 x 20 mm glass tube. For specific fuse ratings, refer to Error! Reference source not found. (Error! Reference source not found.) Cables LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 32 Pin 1 of the other, Pin 2 to Pin 2, etc.). Do not substitute the provided cable with a “null-modem” cable, or any cable that has Pins 2 and 3 crossed and/or connected to other non-corresponding pins. LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 33: Appendix D. External-Control Command Reference

    1D 00011101 -0029 ^] Move on trigger to LED 2 at factory-defined level. Trigger 1E 00011110 -0030 ^^ Move on trigger to LED 3 at factory-defined level. Trigger 1F 00011111 -0031 ^_ LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 34 0000 0000 0000+ bit “unsigned short” (2 bytes), Little Value 0000 0000 0000 Endian 4095 FF0F 1111 1111 0255+ 0000 1111 0015 Rx All Echoed command sequence Completion indicator 0000 1101 <CR> LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 35 Rx All Echoed command sequence Completion indicator 0000 1101 <CR> Start Places the controller in a mode that Tx All 1101 1111 0223 allows a series of filter values to be Loading LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 36 Move on rising edge, then to Filter 0 0000 0010 0002 on signal falling Move on falling edge 0000 0011 0003 Move on falling edge, then to Filter 0 0000 0100 0004 on signal rising LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 37 4095 FF0F 1111 1111 0000 1111 Completion indicator 0000 1101 <CR> Command byte Tx All 1111 0100 0244 Specified Specified filter position 80-8F 1000 0000 0128 Filter’s Galvo B 1000 1111 0143 LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 38 DAC value Nominal filter 11 DAC value Nominal filter 12 DAC value Nominal filter 13 DAC value Nominal filter 14 DAC value Nominal filter 15 DAC value Completion indicator 0000 1101 <CR> LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 39 2. All values greater than one byte (8 bits) transmitted to, and received from, the LAMBDA 421 consist of two bytes ordered in “Little Endian” (least significant byte last) format (see NOTE 3).
  • Page 40 (This page intentionally blank.) LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

  • Page 41: Index

    & precautions unpacking.............5 operational............iii Installation ...............5 safety warnings & precautions......iii instructions ............5 Serial interface connector ........8 introduction..............2 SHUTTER BNC connector ........8 features..............2 STROBE BNC connector........8 general description ..........2 technical support ..........1 LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)
  • Page 42 .............1 electrical............. 23 cables.............. 23 mains fuse............23 unpacking..............5 mains voltage ..........23 USB device connector..........8 power cord ............. 23 lamp life..............23 warranty..............19 output range..........23 weight ..............23 NOTES LAMBDA 421 OPERATION MANUAL – REV. 1.00N (20170623)

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