DeNovix DS-11 Series User Manual
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DeNovix DS-11 Series User Manual

Spectrophotometer/fluorometer
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Rev December 2018

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Summary of Contents for DeNovix DS-11 Series

  • Page 1 Rev December 2018...

  • Page 2: Table Of Contents

    Table of Contents 1. Introduction ....................2. Set up and Safety ..................3. Software Quick Guides ................Software Icons ..................Software Buttons ..................Software Navigation ................Top Bar Overflow Functions ..............4. Report and Graph Screens ..............5. Absorbance Apps: Basic Operation ............
  • Page 3 11. Absorbance Kinetics App ..............12. Absorbance Custom Methods Apps ........... Custom Standard Curve Methods ............Custom Formula Methods ..............13. Fluorescence: Basic Operation ............Best Practices ..................14. Fluorescence: Standard Curve Method Apps ........15. Basic Fluorometer App ................ Excitation Selection and Emission Results ..........
  • Page 4 20. Diagnostics ................... DS-11 Self Test ..................Microvolume Pathlength Verification ............ Verification Check Troubleshooting ............FX- Diagnostics ..................21. Troubleshooting ..................Quick Help ..................... Software Errors ..................22. Customer Support ................

  • Page 5: Introduction

    1. Introduction Product Technology DeNovix Spectrophotometers / Fluorometers are compact instruments with an integrated processor and a high-resolution touchscreen. The software utilizes application specific icons along with the Android™ operating system to provide an intuitive, easy-to-use instrument for both absorbance and fluorescence measurements.

  • Page 6: Set Up And Safety

    2. Set up and Safety 1. Remove all packing materials including the protective cover on the screen. 2. Plug the unit into a 100-240 VAC, 50-60/Hz receptacle using the grounded power supply (CUI MPN: ETSA120330U-P5P-SZ, Transformer Dest Top 12V 40W, Lead free / RoHS Compliant) included with each unit. 3.
  • Page 7 FX Module. In addition, the USB ports support the use of selected DYMO printers and an Opticon OPI 3601 USB barcode reader. USB mouse and keyboard devices may also be used with the DS-11 FX+ series of instruments. Please refer to www.denovix for a list of supported Dymo printers.
...

  • Page 8: Software Quick Guides

    3. Software Quick Guides Software Icons Select All Export Delete Purity Ratio Alert Sort Edit Copy Info Undo Paste Shut Down The Overflow (top action bar) is used to access secondary features such as Select Units, Screen Capture, User Guide and Exit. Progress bars or busy indicators are used to indicate the instrument is in the process of making a measurement.

  • Page 9: Software Buttons

    Software Quick Guides Software Buttons The absorbance apps Blank button is used to establish a reference absorbance within each measurement app. The graph will not display a spectrum for a Blank measurement. The absorbance apps Measure button is used for sample measurements.

  • Page 10: Software Navigation

    Software Quick Guides Software Navigation Apps are launched using Home screen icons. Swipe the Home page to the left for additional icons. The app Run, Report and Graph screens are accessed either by the tabs on the top bar or by swiping the screens left or right.

  • Page 11: Top Bar Overflow Functions

    The Diagnostics app Lamp Reset is used as a means to re- Lamp Reset optimize a lamp when necessary. The Diagnostics app Tech Support feature is used to send Tech instrument related diagnostics files to DeNovix customer Support support. Use the Exit function to close an application. Exit...

  • Page 12: Report And Graph Screens

    4. Report and Graph Screens The measurement apps generally use a three page structure consisting of the Run, Report and Graph screens. The various screens are accessed either by using the top tabs or by swiping the touch screen left or right. The typical Run screen varies significantly between the absorbance and the fluorescence apps.
  • Page 13 Report and Graph Screens • The change will be applied to the current Report and Graph screen rows as well as to the sample information retrieved using the Data app. • Clear highlighted sample records from the both the Report and Graph screens using the Delete icon.
  • Page 14 Report and Graph Screens • Color blocks are used as a legend convention. • Highlight a single row and then tap the Edit icon to add or modify a sample name. The change will be applied to the current Report and Graph screen rows as well as to the sample information retrieved using the Data app.

  • Page 15: Absorbance Apps: Basic Operation

    5. Absorbance Apps: Basic Operation Microvolume Measurements 1. Ensure both top and bottom sample surfaces are clean. 2. Pipette 1 μL of the blank solution onto the lower sample surface. Lower the top arm and tap the Blank button. 3. Remove the solution from both sample surfaces using a clean, dry lab wipe. 4.
  • Page 16 Basic Operation: Absorbance Auto Run Measurements 1. Perform a Blank measurement as previously described and then select the Auto Run checkbox on the app Run screen. Pipette 1 μL of the sample solution onto the lower sample surface. 2. Lower the arm for automatic measurements. Deselect the feature to perform new Blank measurements.

  • Page 17: Typical Absorbance Run Screen

    Basic Operation: Absorbance Typical Absorbance Run Screen • Blank, Measure, AutoRun and Mode: Use the Blank button when measuring the reference solution and the Measure button for samples.Tap the AutoRun box to enable automatic measurements upon lowering the arm. Use the drop- down menu to select either microvolume or cuvette mode measurements.
  • Page 18 Basic Operation: Absorbance Screen Saver • The backlight for the screen will automatically turn off after 10 minutes of inactivity. Simply tap anywhere on the screen to resume the last function. • Although the screen is turned off for the screen saver mode, functions such as the Timer and Kinetics measurements continue in the background.

  • Page 19: Best Practices Absorbance Measurements

    Basic Operation: Absorbance Best Practices Absorbance Measurements • Clean both sample measurement surfaces prior to making the Blank measurement. • Use dH O, not detergents or alcohol, for routine sample measurement surface cleaning • Use a dry lab wipe to remove measurement liquids from both the top and bottom surfaces immediately after each measurement.

  • Page 20: Absorbance Nucleic Acid Apps

    6. Absorbance Nucleic Acid Apps dsDNA ssDNA Microarray The four preconfigured nucleic acid apps are used to quantify nucleic acid samples based upon absorbance values at 260 nm. Quick Protocol 1. Launch the appropriate application from the Home screen. 2. Establish a Blank using the appropriate buffer. 3.

  • Page 21: Dsdna, Rna And Ssdna

    Nucleic Acids: Absorbance dsDNA, RNA and ssDNA The basic nucleic acid apps are optimized for the factor used to calculate sample concentrations. Screen Features • Sample Type: The sample type is used to determine which ng-cm/μL factor is used to calculate the concentration. •...

  • Page 22: Microarray

    Nucleic Acids:Absorbance Microarray The Microarray app is used to quantify nucleic acid samples based upon absorbance values at 260 nm and dyes based upon dye specific analysis wavelengths. Screen Features • Sample Type: The sample type selection of ssDNA is the default option for this application.
  • Page 23 Nucleic Acids: Absorbance Dye List There are 11 preconfigured dye types available for both the Microarray and Labeled Proteins applications. New dye types may be saved using the List Add/ Edit button to the right of the dye list drop-down menu. Refer to the manufacturer of the dye for analysis nm, extinction coefficient and correction factor information.
  • Page 24 Nucleic Acids:Absorbance The following information is required in order to save a new dye to the list: • Dye name • Analysis wavelength • Analysis wavelength specific molar absorption coefficient (M • 260 nm absorbance correction factor • 280 nm absorbance correction factor Preconfigured dyes may not be modified or deleted.
  • Page 25 Nucleic Acids: Absorbance Nucleic Acid Concentration Calculations A modified Beer-Lambert equation is used to calculate concentrations as follows: c = (A * e)/b c = the nucleic acid concentration in ng/microliter A = the absorbance in AU e = the wavelength-dependent factor in ng-cm/microliter b= the pathlength in cm (10 mm) Baseline Correction Baseline corrections correct for offsets due to sample particulates.
  • Page 26 Nucleic Acids:Absorbance Troubleshooting Purity Ratios Issue Probable Causes/ Solutions Dirty measurement surfaces or improper Blank. Clean the surfaces, measure a new Blank using fresh source of 0 or sample buffer. Use the same solution (water or buffer) the sample is in for the blank measurement. Low 260/230 Presence of residual extraction reagent (ex.
  • Page 27 Nucleic Acids: Absorbance Ratio Alerts The DS-11 software v3.0 and above displays alerts icons within the dsDNA, RNA and ssDNA apps for samples with purity ratios outside of specified minimum and maximum limits. Tapping on the icon will pop-up a message with error specific information.

  • Page 28: Absorbance Purified Protein, Peptide Apps

    7. Absorbance Purified Protein, Peptide Apps Protein Labeled Peptides A280 Proteins Quick Protocol 1. Launch the appropriate application from the Home screen. 2. Establish a Blank using the appropriate buffer. 3. Enter a sample name. 4. Select a sample type. •...

  • Page 29: Protein A280

    Purified Protein, Peptides: Absorbance Protein A280 The Protein A280 application is used to quantify samples based upon absorbance values at 280 nm. Screen Features • Sample Type: The sample type and associated mass extinction coefficients options include: Use the 1A=1mg/ml option when neither the E1% nor Ext Coeff and MW for the purified protein sample are known.
  • Page 30 Purified Protein, Peptides: Absorbance Baseline Correction Bichromatic normalizations correct for baseline offsets due to sample particulates. If a baseline correction is not used, the sample spectrum may be offset from the baseline, resulting in a change in the reported sample concentration.

  • Page 31: Labeled Proteins

    Purified Protein, Peptides: Absorbance Labeled Proteins The Labeled Protein application is used to quantify protein samples based upon absorbance values at 280 nm as well as Dyes and Fluorophores at specific analysis wavelengths. Screen Features • Sample Type: The sample type and associated mass extinction coefficients options include: Sample Type E1% at 280 nm for 10 mm pathlength...
  • Page 32 Purified Protein, Peptides: Absorbance Protein Type List New Protein types using either user-defined E1% or MW and Ext. Coeff values may be saved using the List Add/Edit button to the right of the sample type drop- down menu. • The new sample types may be used by any account holder. •...

  • Page 33: Peptides

    Purified Protein, Peptides: Absorbance Peptides The Peptide app is used for low concentration samples which do not have aromatic residues such as Trp, Tyr or Cys-Cys disulphide bonds. The Protein A280 app is recommended when the sample does contain aromatic rings. Screen Features •...
  • Page 34 Note: The Peptide app uses E 0.1% values whereas the Protein A280 app uses E 1% values. The DeNovix software reports concentrations in mg/mL units for the Protein A280, Labeled Protein and Peptide applications. The concentration is determined based upon the absorbance value at the analysis wavelength used...
  • Page 35 Purified Protein, Peptides: Absorbance Ratio Alerts The DS-11 software v3.0 and above displays alerts icons within the Protein A280 for samples with A260/280 purity ratios outside of specified maximum limits. Tapping on the icon will pop-up a message with error specific information. In general, pure protein samples will have a ratio below 0.57.

  • Page 36: Absorbance Colorimetrics App

    8. Absorbance Colorimetrics App Bradford Lowry Pierce 660 Refer to the manufacturer of the assay reagent for assay specific instructions. Quick Protocol 1. Launch the app from the Home screen. 2. Select the assay type on Run screen. Navigate to the Standard Curve screen.

  • Page 37: Generating A Standard Curve

    Colorimetrics: Absorbance Generating a Standard Curve 1. Use the drop-down menu on the Std Curve screen to select New Curve or to import a saved standard curve or standard curve concentration list. 2. If the New Curve option is selected, tap the Enter Standards button to enable a pop-up box to enter a list of standard concentrations for the current measurement session.
  • Page 38 Colorimetrics:Absorbance Line Fit The average of the replicates is used to determine a best-fit trend line of the data set. The trend line equation is applied to the sample absorbance values to calculate concentrations. Options include: • Linear • 2 Order •...
  • Page 39 Colorimetrics: Absorbance • Use the List Add/Edit button to the right of the curve list drop-down menu to save a curve or concentration list. Editing a Standard Curve • The curve is in an editable state when the Lock icon is green. •...

  • Page 40: Bca

    Colorimetrics:Absorbance The bicinchoninic acid assay (BCA assay) is used to quantify protein by measuring the Cu-BCA chelate formed in the presence of protein. • The reaction product is measured at 562 nm using a baseline correction at 750 Bradford The Bradford assay is based upon a protein-induced absorbance shift of a Coomassie Blue dye as a method to determine protein concentration.

  • Page 41: Absorbance Uv-Vis App

    9. Absorbance UV-Vis App UV-Vis The UV-Vis app is used to monitor up to 6 specific analysis wavelengths. Absorbance values for all wavelengths from the user selected wavelength ranges are saved for each measurement and can be exported as a .csv file using the Export function from the Report or Graph screens.
  • Page 42 UV-Vis Wavelength Range Selection There are two wavelength ranges available for the UV-Vis app. Use the checkbox feature to select between the 220 to 750 nm or the 190 to 840 nm options. Changing ranges will automatically save and then clear current results. A new Blank is required when switching ranges.

  • Page 43: Absorbance Od 600 App

    10. Absorbance OD 600 App OD 600 This application is generally used to determine the optical density of microbial cell cultures at 600 nm. Conversions to cells per mL are based upon a user- defined factor. Quick Protocol 1. Launch the app from the Home screen. 2.
  • Page 44 OD 600 Unique Features • A600: The OD 600 is a measure of the light scattered by the cell suspension solution. Reported values may differ for the same sample measured on different spectrophotometer systems as values are dependent on both the cell type and the optical configuration of the spectrophotometer.

  • Page 45: Absorbance Kinetics App

    11. Absorbance Kinetics App Kinetics The Kinetics application is a cuvette mode only application available for the DS-11+ and DS-11 FX+ models. Quick Protocol Launch the app from the Home screen. Use the Create Method button on the Run screen to define the parameters of the method.
  • Page 46 Kinetics:Absorbance Define Method Screen Features • Method Name: A unique name is required for each method. • Analysis nm: Enter the primary wavelength of interest for the method. The absorbance value at this wavelength will be plotted versus time on the Trend plot.
  • Page 47 Kinetics: Absorbance Run Screen Features • Method: Select the method of choice using the drop-down menu. Use the List Add/Edit button to the right of the drop-down to create or edit methods. All methods are available to all accounts. However, only the account holder or an administrator may edit or delete a method.
  • Page 48 Kinetics:Absorbance Sample Solution Temperature • The solution in the cuvette may not reach the set point as quickly as the heater. Use the Run screen timer as a means of monitoring the elapsed warm-up time. Below are estimated warm-up times. Solution Start Temp 4ºC Room Temperature...

  • Page 49: Absorbance Custom Methods Apps

    12. Absorbance Custom Methods Apps Std Curve Methods Formula Methods These applications are used to create, save and access user-defined methods. Quick Protocol Launch the applicable Custom Methods app from the Home screen. Use the Create Method button on the Run screen to define the parameters of the method.

  • Page 50: Custom Standard Curve Methods

    Custom Methods:Absorbance Primary Settings Each new method requires a unique name. In addition, the following parameters must be defined: • Analysis nm: Enter the primary wavelength of interest for the method. • Absorbance values at the analysis nm will be used to generate a curve for Standard Curve methods.

  • Page 51: Custom Formula Methods

    Custom Methods: Absorbance Custom Formula Methods In addition to the primary analysis wavelength, the Formula Methods app enables the user to include up to 4 additional results for each measurement. The result fields may be used to monitor additional specific wavelengths or have the software perform routine calculations based upon absorbance values measured at specific wavelengths.
  • Page 52 • 10 mm pathlength equivalent absorbance values are used and reported for microvolume measurements. Cuvette IQOQ mode • The cuvette IQ/OQ 10 mm option is exclusively for use with the Formula Methods app when running the DeNovix suggested IQOQ procedure. Contact customer support for more information. 
...

  • Page 53: Fluorescence: Basic Operation

    13. Fluorescence: Basic Operation The DeNovix spectrophotometer/fluorometer software versions 2.0 and above include both absorbance and fluorescence apps. The fluorescence app icons will appear on the first page of the Home screen for the integrated DS-11 FX and DS-11 FX+ models. The inactive versions of the icons will appear on the second page for DS11 or DS-11+ units when a FX Module USB accessory is not detected.

  • Page 54: Best Practices

    Fluorescence Basic Operation Best Practices • Ensure all sample concentrations in the thin walled assay tubes fall within the limits of the assay or reagent kit for accurate results. • Use 200 μL volumes in a clear thin walled PCR tube. •...

  • Page 55: Fluorescence: Standard Curve Method Apps

    • DeNovix dsDNA Broad Range • Qubit™ dsDNA BR • DeNovix dsDNA High Sensitivity • Qubit™ dsDNA HS Fluoro • DeNovix dsDNA Ultra High Sensitivity • Quantifluor dsDNA • Quant-iT™ PicoGreen dsDNA • Quantifluor One • Quant-iT™ dsDNA HS dsDNA •...
  • Page 56 • Optional: Enter a dilution factor to be applied to the reported concentration. 6. Quantitate a sample using the Measure button. Refer to the manufacturer of the assay reagent for assay specific instructions. Note: •DeNovix and Qubit assays will have predefined standard concentrations and specified units.
  • Page 57 Standard Curve Methods: Fluorescence Run Screen- Assay and Curve Selections Assay Selection • Select an assay from the drop-down menu in the left panel. • Preconfigured entries are optimized in regards to excitation source, assay units, and trend line analysis according to the reagent kit manufacturer's recommendations.
  • Page 58 Standard Curve Methods: Fluorescence Curve Options Generate New Curve • Choosing this option will pop-up a dialog window used to enter the concentration values for the standards. • The standard concentrations for preconfigured Qubit® assays are hardcoded into the software. The standard concentrations for all other assays are determined by the user at the time of the assay.
  • Page 59 Standard Curve Methods: Fluorescence Run Screen- Standard Curve View • The Stds button will pop-up the Edit Concentrations for Standard Curve window. • The Delete and Undo icons are used to manage the RFU values within the table. • The Save button will pop-up the Save Values window. Either concentration values or concentration values with the associated RFU values may be saved.
  • Page 60 Standard Curve Methods: Fluorescence Run Screen- Sample Results View • Use the Samples radio button to display the measurement results view. • The samples Measure button will be disabled until one replicate of at least two standards are measured using the standard curve view. •...
  • Page 61 Standard Curve Methods: Fluorescence New Standard Curve Assays/Methods Each new method requires a unique name. In addition, the following parameters must be defined: Excitation Source • Use LED Radio buttons to select the excitation source. • The LED Wizard may be used to determine the optimal excitation source if not known.

  • Page 62: Basic Fluorometer App

    15. Basic Fluorometer App Basic Fluorometer This app enables the DS-11 FX series of instruments to be used as a versatile fluorometer for applications such as dye QC checks and assay development. The app is especially useful for fluorophores with significant Stoke shifts as it allows the user to excite a sample with one LED and get RFU results from multiple emission channels.

  • Page 63: Excitation Selection And Emission Results

    Basic Fluorometer Excitation Selection and Emission Results • Select a fluorophore from the drop-down menu in the left panel. The excitation source is automatically determined for preconfigured fluorophores. • The selected LED is the only excitation source used during a measurement. However, the emission RFU will be collected for each longer wavelength emission filter set.

  • Page 64: Data Export And Print Options

    Refer to the "Network Folder Set-up" section for more information. • To open an exported .csv file on a computer, the DeNovix instrument and the computer must both be set to use either period or comma number formats. The DS-11 FX number format is determined by the language selected using the Settings app.

  • Page 65: Export Via Email

    Data Export and Print Defining a New Format: • Provide a title for the format. • Hold the arrow icon and drag a column up or down to change the order of the columns. • Touch on the column name and swipe it left or right to exclude or include it in the exported report.

  • Page 66: Export To A Network Folder

    Data Export and Print Email Set-up 1. Ensure that the instrument is connected to WiFi or Ethernet. 2. Swipe the Home screen to the left to navigate to the second page, launch the Email app and follow the prompts using the Manual setup option. 3.
  • Page 67 Data Export and Print Settings App • Permission to setup a network folder for the General Account as well as standard user accounts are enabled or disabled by an administrator using the Permissions option in the Settings app. "Enabled" is the default selection.

  • Page 68: Usb

    Data Export and Print • Log in: Enter the network folder username and password required to access the selected network folder. • Anonymous: No user name or password required. • File (SMB only): Upload a configuration file (file extension .prp ) from a USB device.
  • Page 69 Data Export and Print Auto Export Icon When the auto export feature is selected, a status icon will appear at the top right of the screen after the first measurement. Export in Progress Export Completed Export Failure • Automatic export of every sample measurement to a network folder is enabled on a per user account basis.

  • Page 70: Print Options

    Printers icon in the left pane to search for nearby printers. The current software supports approximately 90% of printers on the market. DeNovix will continue to add new drivers to accommodate as many new printers as possible in future software updates but cannot guarantee compatibility with all printers.
  • Page 71 Data Export and Print DYMO Printers • The software enables the use of three DYMO paper or label sizes: • 4x6 labels (DYMO 4XL only) and 2.5 inch paper rolls are used to print screen captures and selected sample results from the Report or Graph screens.

  • Page 72: Utility Apps And Functions

    • This account is used to manage instrument settings. It is not used within the measurement applications. • The primary administrator account is not initially password protected. A password may be applied at any time. Contact DeNovix if help is required to reset the primary administrator account password. • General Account •...
  • Page 73 Contact the holder of an administrator account if a standard user account password is forgotten. Contact DeNovix customer support or your local distributor if the Primary Administrator password is forgotten. Please have the serial number of the instrument available when requesting help for the Primary Administrator password reset.

  • Page 74: Data App

    Utility Apps Data App This app allows the user to search for past data using either a Advanced Search or Quick Search option. Data can then be added to the Report and Graph screens for review and export. In addition, data may be archived from the Search Screen.

  • Page 75: Export Vs Archive

    Utility Apps • Once the filter criteria have been selected, tap the Search button at the bottom of the screen. Tap any column header to sort the results list. • Highlight the sample(s) of interest and tap the Add to Report button to include the samples on the Report and Graph screens.
  • Page 76 Utility Apps • The Archive Selected Samples function available from the Run and Report screen overflow menus creates a .csv file with sample data that can be exported to a folder entitled ds11 on a FAT32 formatted USB flash drive or saved to a network folder.

  • Page 77: Settings App

    USB flash drive or a network folder. • Backup files include a DeNovix specific extension and may not be opened on a computer. The files may be restored back onto the DS-11 at any time.
  • Page 78 Utility Apps Date/Time • Select manual or automatic date and time options. Language • Although the Android system provides translations for some routine functions in many languages, the majority of the app specific text will be available in a limited number of language options. •...
  • Page 79 Utility Apps Sound • Volume controls are available for media and timer alarms. Storage • Displays storage statistics. WiFi To enter static IP addresses : 1. Ensure the WiFi is on. Select WiFi from the Settings list. 2. Tap the Add icon on the right side of the top action bar. 3.

  • Page 80: Updater App

    Updater App This app is used to update all apps to the most current available versions. The latest updates are available at www.denovix.com. For instruments without internet access, a zip file with the latest updates may be downloaded from the website onto a FAT32 formatted USB flash drive.

  • Page 81: Lab Tool Apps

    18.Lab Tool Apps Calculators Timer Calculator App Three calculators are included within the app. Use the tabs in the top action bar to navigate between the options. Oligo Calculator This calculator analyzes a user-inputed ssDNA or RNA sequence of interest. •...

  • Page 82: Timer App

    Lab Tool Apps Scientific Calculator Use this calculator for standard mathematical and scientific functions. Dilution Calculator This calculator is a useful tool to calculate how much of a stock solution should be added to a buffer solution to result in a final specified solution concentration. •...

  • Page 83: Maintenance

    19. Maintenance Cleaning 1. Pipette 2 μL of dH 0 onto the bottom sample surface. Lower the upper arm. Wait 1-2 minutes. Routine 2. Wipe away the water from both the upper and lower sample surfaces with a dry, lint-free lab wipe. Between Use a dry, lint-free lab wipe to remove samples from both the Measurements...

  • Page 84: Solvent Compatibility

    Exception: Do not use Hydrofluoric Acid (HF) as the fluoride ion will dissolve the quartz fiber optic cable. Customer Support Contact DeNovix Customer Support at 1-302-442-6911 or info@denovix.com additional help is required. Please include the serial number of your instrument when contacting Customer Support by email.

  • Page 85: Diagnostics

    20. Diagnostics FX Diagnostics Diagnostics Although the DS-11 FX propriety SmartPath® technology enables accurate microvolume pathlength control and eliminates the need for routine recalibration, labs may choose to run the Diagnostics app to verify that the instrument is working within specifications. DS-11 Self Test •...

  • Page 86: Microvolume Pathlength Verification

    Diagnostics Microvolume Pathlength Verification The DeNovix LC-NA verification fluid is supplied as single use vials composed of a non-toxic solution of aqueous nicotinic acid. The solution is used to verify that the microvolume pathlengths are within specifications. Procedure 1. Ensure both sample measurement surfaces are clean. Refer to the general cleaning instructions in the chapter entitled "Maintenance"...

  • Page 87: Verification Check Troubleshooting

    Diagnostics Verification Check Troubleshooting Issue Probable Causes/ Solutions Dirty measurement surfaces or improper Blank. Clean the surfaces, measure a new Blank using fresh source of dH Low Absorbance and restart the procedure using a new ampule of LC-NA. Values Multiple measurements made using the same aliquot. Use a fresh aliquot for each measurement.
  • Page 88 Note: Only the LC-NA standard available from DeNovix and its authorized distributors should be used for the verification check. No other sources of nicotinic acid are tested and validated for use with the DS-11 verification procedure.

  • Page 89: Fx- Diagnostics

    • The software tests that each LED is firing and each detector is functioning properly. Results will be reported as Pass/Fail. Technical Support Contact DeNovix Customer Support if additional help is required. For US customers, please email the results directly to info@denovix.com using the FX- Diagnostics app Overflow Tech Support function.

  • Page 90: Troubleshooting

    21. Troubleshooting Quick Help Most issues concerning accuracy, reproducibility, negative spectra and low nucleic acid purity ratios are sample or technique related and are resolved by following the suggestions below: • Establish a new Blank using the appropriate buffer. • Ensure that the sample isolation procedure is optimized and that samples are purified when required prior to making absorbance measurements.
  • Page 91 Cuvette Mode • Use cuvettes with Z heights of 8.5 mm. • Use quartz or UV transparent cuvettes for UV range measurements. • Ensure cuvettes are clean prior to use. • Fill cuvettes to manufacturer's specifications. • Confirm the mode selection has been changed on the Run screen to the proper cuvette pathlength prior to a measurement.

  • Page 92: Software Errors

    Opticon OPI 3601 USB barcode scanners. For accessory product troubleshooting and warranty information, please contact the manufacturer of the product. DeNovix support is limited to the specific use of the product with a DS-11 FX instrument. Please refer to www.denovix...

  • Page 93: Customer Support

    Outside of the US, please contact your local distributor for assistance. Warranty All product(s) and accessories sold by DeNovix Inc. are under warranty against manufacturing defects in parts. Disclaimer All information in this document is for reference purposes only. DeNovix Inc.

This manual is also suitable for:

Ds-11+Ds-11 fx+

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