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Table of Contents
Summary of Contents for Fluidic Analytics Fluidity One
- Page 1 IFU 004 User Guide Issue 4...
- Page 2 Fluidic Analytics products have been designed to be safe in accordance with the operating instructions. If the equipment is not used in the manner specified in the operating manual the equipment may be impaired and warranty may be void, warranty exclusions include: •...
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Page 3: Table Of Contents
Chemical safety ..............................6 2.5. Manual handling .............................. 6 2.6. Power requirements ............................6 Instrument specifications.......................... 7 3.1. Fluidity One Instrument Purpose ....................... 7 3.2. Chips ................................... 7 3.3. Reagent cartridge............................8 User Training ............................... 9 Installation ..............................9 5.1. -
Page 5: General Information
Cambridge CB1 8DH This manual provides a guide for the installation and upkeep of the Fluidity One by trained service personnel and users. All users must familiarise themselves with the safety guidelines and general operation information sections of this manual prior to use. -
Page 6: Safety Information
If you wish to discard electrical and electronic equipment (EEE), please contact Fluidic Analytics Ltd for further information. The Fluidity One contains a Lithium Coin Cell Battery with the following warnings: REACH Regulation (EC) No 1907/2006 Substances of Very High Concern (SVHC) Information... -
Page 7: Hazard Summary
The Fluidity One uses an in built air compressor to control liquid movement through the instrument. The Fluidity One contains a UV light source. This does not present a danger to users provided the unit is not opened. The Fluidity One contains a fan. This does not present a danger to users provided the unit is not opened. -
Page 8: Chemical Safety
(Reference: MSDS001) 2.5. Manual handling The Fluidity One weighs 16 kg and should be carried with the appropriate level of care. 2.6. Power requirements The power pack is usable from 100-240VAC, 50-60Hz. The unit requires an earth. Only use... -
Page 9: Instrument Specifications
3.1. Fluidity One Instrument Purpose The Fluidity One uses Microfluidic Diffusional Sizing to size and quantify unlabelled, native protein in solution in less than 15 minutes. This can be used in a laboratory environment for routine protein sizing, protein quality analysis, study of protein conformation, aggregation or oligomerization and analysis of protein interactions. -
Page 10: Reagent Cartridge
Reagent cartridge Reagent cartridges are supplied with colour coded connections and can be connected to the Fluidity One instrument as shown. Do not disconnect the reagent cartridge while an experiment is running. Reagent cartridges are disposable and should not be re-filled by users. -
Page 11: User Training
Prior to placing the instrument ensure the work surface on which the instrument is to be placed is clear and clean. Do not place the Fluidity One adjacent to instruments that could cause excessive vibrations, such as centrifuges, vortex mixers etc. -
Page 12: Transportation And Handling
The instrument should be operated at room temperature (15-25°C) Take care to position it away from other equipment which may give off excessive heat, e.g. fridges, freezers, refrigerated centrifuges or thermal cyclers. 5.6. Connections The Fluidity One connections are made as shown below. - Page 13 Front USB port to download results Rear USB port to download results Rear USB 2.0 B port – for use by Fluidic Analytics engineers during assessment only. Ethernet connection – can be used to connect the instrument to a network where WiFi is not available.
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Page 14: Operation
Operation 6.1. Manual checks Check the instrument to ensure there is no packaging material present. Connect the reagent cartridge as shown in section 6.5. Ensure there is no obstruction in the chip insert slot. 6.2. Turning on and off Connect the power supply to the instrument, then to the mains. To turn the instrument on, press the power button on the back of the instrument. -
Page 15: Pipetting Onto Chips
6.3. Pipetting onto chips The pedestal indicated in the picture below can accept 5-10 µL of sample. A larger volume can lead to overflow and incorrect insertion of the sample onto the chip. If overflow occurs, discard the chip and perform the test again. 5 µL is the recommended volume. -
Page 16: Replacing Reagent Cartridges
6.5. Replacing reagent cartridges The reagent cartridge is located in a compartment at the top rear of the instrument as shown below. When the reagent level stated in the side bar menu is 5% or lower, or if an experiment result gives an error code stating reagent cartridge is low, the reagent cartridge should be replaced as per the instructions below. -
Page 17: Starting The Measurement
10. Type in the desired cartridge identifier – today’s date is recommended to record the date of the change, but this can be any value you choose. Press “update cartridge ID” to save. 11. The low cartridge % will still be displayed until an experiment is run or the instrument is restarted. - Page 18 The measurement may then be tagged with metadata related to the experiment using the Edit Tag button, this includes pre-prepared tags like Concentration and Buffer or you can create your own. Tags can be edited prior to, during, and after the run. Any tag data will be included in the export file where results are exported.
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Page 19: Calibration
6.7. Calibration Calibration can be performed to improve the accuracy of concentration measurements but is not required for most proteins and peptides, as the degree of labelling is consistent for the majority of samples. Calibration will not affect the size measurement as the rate of diffusion, directly measured by the instrument, has a well defined physical relationship as described by the Stokes-Einstein equation. -
Page 20: Viewing Results
Viewing Results Following the measurement the results are displayed on screen. You may begin a new experiment, finish the session and return to the home screen or access advanced results and view the measurement trace. Press “Finished” to return to the home screen. Press “New experiment”... - Page 21 Press “View trace” to see the trace plot for this measurement (see www.fluidic.com for help with interpreting traces). If there were any errors during the run, these will be indicated in the “System Events” box on the left hand side. Touch on this box to expand and view more detail on the errors. See www.fluidic.com for help with interpreting error codes.
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Page 22: Shut Down
Shut down Before shutting down the instrument ensure no measurements are in progress. To shut down the Fluidity One press and hold the power button on the back of the instrument for 5 seconds. It is recommended to turn the instrument off overnight. -
Page 23: 11.2. File Formats Available For Results Download
The only maintenance which may be performed by instrument users is changing the cartridge (see section 6.5). All other maintenance, adjustments or fault repairs must be performed by Fluidic Analytics staff or an authorized agent only. To report a fault, request a maintenance visit, or for information on service contracts contact •... - Page 25 Document reference: IFU004 Issue 4 +44 (0)1223 560 432 www.fluidic.com help@fluidic.com...