Oxford Optronix OxyLite Pro User Manual
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Oxford Optronix OxyLite Pro User Manual

Continuous in vivo and in vitro combined po2 and temperature monitoring
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OxyLite Pro User Manual
User Manual
For models
OxyLite™ Pro
OxyLite™ Pro XL
Continuous in vivo and in vitro combined
pO
and temperature monitoring
2
Revision 1.2 | May 2015
1

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Summary of Contents for Oxford Optronix OxyLite Pro

  • Page 1 OxyLite Pro User Manual User Manual For models OxyLite™ Pro OxyLite™ Pro XL Continuous in vivo and in vitro combined and temperature monitoring Revision 1.2 | May 2015...
  • Page 2 +44 (0)1235 821 678 Primary website: www.oxford-optronix.com Sales email: sales@oxford-optronix.com Support website: www.oxford-optronix.com/support/supp_oxylite.htm Support email: support@oxford-optronix.com OxyLite™ and OxyFlo™ are registered trademarks of Oxford Optronix Ltd. © Oxford Optronix Ltd. 2015. All rights reserved. Revision 1.2 | May 2015...

  • Page 3: Table Of Contents

    EMC Compliance – EC Declaration of Conformity ......6 OxyLite Pro Symbols..............6 Definitions ..................6 Summary of Warnings for the OxyLite Pro Monitor ......7 Summary of Cautions for the OxyLite Pro Monitor ......7 Summary of Cautions for OxyLite Pro Sensors......8 NTRODUCTION General Description ..............
  • Page 4 Figure 1: Front view of the OxyLite Pro ............. 13 Figure 2: Rear view of the OxyLite Pro............14 Figure 3: Side-view of OxyLite Pro, showing open sensor connector bay. 15 Figure 4: The NX-PSU universal AC adapter ..........15 Figure 5: An OxyLite Pro senso ..............

  • Page 5: Safety Information

    (i.e. in vivo), physiological fluids, cell cultures or other samples (i.e. in vitro). OxyLite Pro is suited to the determination of tissue or cell culture hypoxia as the measurement technique is particularly sensitive at low tissue pO...

  • Page 6: Emc Compliance - Ec Declaration Of Conformity

    Alternating current Direct current Digital output Analogue outputs Table 1: OxyLite Pro symbols Definitions A warning indicates the possibility of injury to the operator. WARNING A caution indicates a condition that may lead to equipment damage and/or malfunction.

  • Page 7: Summary Of Warnings For The Oxylite Pro Monitor

    OxyLite Pro User Manual Summary of Warnings for the OxyLite Pro Monitor Do not attempt to open the OxyLite Pro. There are no user- serviceable parts inside. There is a risk of electrical shock or other injury or permanent damage to the monitor.

  • Page 8: Summary Of Cautions For Oxylite Pro Sensors

    For safe storage of OxyLite Pro sensors always refit the protective black cover when a sensor is not in use. CAUTION DO NOT drop, stretch or ‘kink’ any part of an OxyLite Pro sensor. Permanent damage may result. CAUTION DO NOT soak or immerse OxyLite Pro sensors in corrosive solution.
  • Page 9 CAUTION The effect of exposure to heat, radiation, glutaraldehyde, ETO gas or plasma on the performance and measurement accuracy of OxyLite Pro sensors has not been validated. CAUTION Revision 1.2 | May 2015...

  • Page 10: Introduction

    ‘oxygen tension’ or as ‘dissolved oxygen’. OxyLite Pro is an optical device based upon the principle that the presence of molecular dissolved oxygen in tissues or fluids can terminate (quench) light emitted by a fluorescent compound (dye).

  • Page 11: List Of Key Features

    OxyLite Pro monitor upon connection. The OxyLite Pro is set up and controlled using commands issued via its touch sensitive user interface display. Continuous pO...
  • Page 12  Multi-channel productivity The OxyLite Pro is available in dual or four-channel models to suit all needs including the simultaneous monitoring of oxygen from multiple tissue sites (e.g. comparison of pathological versus control tissue sites), or multiple in vitro samples.

  • Page 13: The Oxylite Pro Monitor

    OxyLite Pro User Manual The OxyLite Pro Monitor Figure 1: Front view of the OxyLite Pro 1. Sensor connector bay (one on each side for OxyLite Pro XL model) 2. Power-on indicator light. 3. Touch-screen display (with adjustable viewing angle).

  • Page 14: Figure 2: Rear View Of The Oxylite Pro

    2. Low voltage power inlet (use ‘NX-PSU’ universal AC Adapter only) 3. Analogue output connectors: Ch 1 / Ch 2 (common to all OxyLite Pro models) Ch 3 / Ch 4 (OxyLite Pro XL model only) 4. USB serial output for optional direct data streaming to LabChart® Pro 5.

  • Page 15: Figure 3: Side-View Of Oxylite Pro, Showing Open Sensor Connector Bay

    Figure 4: The NX-PSU universal AC adapter NOTE: Use ONLY the universal AC adapter provided by Oxford Optronix. Failure to do so may result in irreparable damage to the OxyLite Pro monitor and may cause harm to the user. Revision 1.2 | May 2015...

  • Page 16: Oxylite Pro Sensors

    (protected beneath a removable black tubular protective cover) and at the other end in a connector plug that attaches to the OxyLite Pro. The connector plug is colour-coded with a blue band.

  • Page 17: Figure 5: An Oxylite Pro Senso

    NOTE: When replacing the adapter and in order to achieve a secure ‘grip’ on the sleeving, ensure that the threaded rear section is twisted tightly. Figure 5: An OxyLite Pro sensor (‘NX-BF/OT’ type depicted) Revision 1.2 | May 2015...
  • Page 18 The sensor ID is printed at the connector (plug) end of each sensor and is also found on the sensor packaging. It is not possible for the user to calibrate OxyLite Pro sensors. SENSOR-RELATED CAUTIONS: OxyLite Pro sensors should be HANDLED WITH CARE.

  • Page 19: Accessories

    Product Code Product Description High-density 15-pin D-connector to 4 BNC data NX-BNC adapter cable for OxyLite Pro or OxyFlo Pro (1m) LabChart® Pro software (PC/Windows® only), incl. 1 LABCHART_PRO user license and 5 years free updates 12 channel analogue output data recorder and PC VALUE ADC software, by Dataq Inc.

  • Page 20: Monitor Setup And Use

    Unpacking and Inspection Immediately notify Oxford Optronix Ltd. or your local distributor if the outer packaging or carton is wet or damaged in any way. Unpack the OxyLite Pro and its components, ensuring that all items listed on the enclosed packing list / dispatch note are present.
  • Page 21 3.5). Connecting the NX-PSU universal AC adapter to the DC POWER IN inlet at the rear of the OxyLite Pro, having already powered it via its On/Off switch MAY trigger the surge protection circuit within the OxyLite Pro. If the OxyLite...

  • Page 22: Connecting / Disconnecting Sensors

    OxyLite Pro User Manual Connecting / Disconnecting Sensors The OxyLite Pro supports pre-calibrated sensors featuring integrated ‘EEPROM’ electronic chips to provide true plug and play convenience. The electronic chip contains sensor-specific calibration information which is read and loaded by the monitor upon connection.

  • Page 23: The Touch-Screen Display

    Figure 7: The touch screen display with no sensors connected Following monitor start up the display will initially show the default screen (OxyLite Pro XL model in this example). Figure 8: The touch screen display with 2 sensors connected The screen will concurrently display a trace and a numerical value of pO2 for each sensor connected.

  • Page 24: Table 3: Touch Screen Default Toolbar Icons

    OxyLite Pro User Manual Description of icons: The toolbar displays sensor summary information per channel in use, including the 6-digit sensor ID, days remaining until ‘recommended use-by date’ (35 in this example), and a graphical representation of accumulated sensor longevity remaining (bar).

  • Page 25: Figure 9: The Touch Screen Settings Toolbar

    OxyLite Pro is powered up. Displays the pulse rate (sampling rate) options screen. Changes to this function are NOT saved and will return to sensor defaults the next time the OxyLite Pro is powered up. Displays the screen ‘swipe’ sensitivity controls, used to set the sensitivity of the touch screen to swipe motions for controlling x-axis and y-axis scaling.

  • Page 26: Figure 10: The Touch Screen Sensor Status Dialogue

    OxyLite Pro User Manual Displays the system date for optional adjustment. Changes to this function are saved and will apply the next time the OxyLite Pro is powered up. Displays software/firmware version information. Table 4: Touch screen settings toolbar icons...

  • Page 27: Table 5: Sensor Status Dialogue Icons

    OxyLite Pro User Manual Accumulated sensor longevity remaining (hours).  ‘ ’ indicates that the sensor remains within its accumulated longevity limits. ‘X’ indicates that sensor longevity has been exceeded. See further details below. Indicator for presence/integrity of sensor oxygen-sensitive ‘tip’.

  • Page 28: Sensor Shelf Life And Accumulated Longevity

    Oxygen sensors are provided with a 2-year shelf life. Total shelf life is defined as the time from factory calibration until sensor expiry and is independent of sensor usage. The OxyLite Pro will not accept a sensor that has exceeded its 2-year shelf life.

  • Page 29: Placement Of Sensors In Vivo Or In Vitro

    OxyLite Pro User Manual The accumulated longevity status of a sensor is displayed schematically on the display toolbar and can be called up via the sensor status dialogue (see above). Upon reaching an accumulated usage of 48 hours, a warning is displayed on the touch screen display to alert the user that a further 2 hours of ‘grace’...

  • Page 30: Temperature Compensation

    (140 mmHg) + (1.5% x 10 x 140) = 140 mmHg + 21 mmHg = 161 mmHg. i.e. in this instance the OxyLite will under read by 21 mmHg. To overcome this limitation, the OxyLite Pro is capable of real-time temperature compensation across a limited temperature range.
  • Page 31 Independent temperature compensation per channel All OxyLite Pro channels feature an integrated temperature input. The measured oxygen value is automatically and continuously compensated where the channel is occupied by a combined oxygen/temperature sensor using the temperature measured by the integrated sensor thermocouple.

  • Page 32: Digital (Usb) Data Output

    Compatibility requires a dedicated and free software Add-on for LabChart (the ‘Device Enabler’*), which supports automatic recognition of the Oxford Optronix monitor, the use of multiple Oxford Optronix monitors simultaneously and provides a choice of pre- loaded configuration settings specifically tailored to our monitors.

  • Page 33: Analogue Data Outputs

    The dual-channel OxyLite Pro requires one such cable (carrying data for channels 1 and 2), while the 4-channel OxyLite Pro XL model requires two such cables (carrying data for channels 1 and 2, and 3 and 4, respectively).

  • Page 34: Table 7: Oxylite Pro Analogue Output Unit Conversion

    OxyLite Pro User Manual The following table summarizes the unit conversion parameters required for data acquisition via the analogue outputs at the rear of the OxyLite Pro monitor when using third-party data recorders (factory defaults shown): Parameter Temperature Output voltage range 0 –...

  • Page 35: Care Of Oxy Lite Pro

    4. C ARE OF Use of OxyLite Pro OxyLite Pro should only be operated on a secure, flat, horizontal surface and in ambient temperatures of 15°C to 30°C. Cleaning and Storage of OxyLite Pro The monitor enclosure may be surface-cleaned by wiping lightly using a soft cloth dampened with a commercial, nonabrasive cleaner.

  • Page 36: Cleaning, Disinfection And Sterilization Of Sensors

    Cleaning OxyLite Pro sensors are manufactured and packed in a controlled (clean) environment. The tips of OxyLite Pro sensors can be cleaned after use by gentle wiping with water, or saline-soaked cotton wool / cotton gauze. The oxygen sensing tips are fragile and can be damaged by attempts to clean them without due care and attention.

  • Page 37: Disposal Of Sensors

    CAUTION Sterilization OxyLite Pro sensors are NOT supplied pre-sterilized. OxyLite Pro sensors are NOT capable of withstanding sterilisation by dry or moist heat (autoclaving). The materials and components used in OxyLite Pro sensors may be compatible with exposure to ETO (ethylene oxide) gas.

  • Page 38: Troubleshooting And Maintenance

    ROUBLESHOOTING AND AINTENANCE Troubleshooting If you experience a problem using the OxyLite Pro that you are unable to correct by reconnecting the sensor(s) and/or turning off and turning back on the monitor, please contact Oxford Optronix technical support (see p2 of this document).
  • Page 39 OxyLite Pro User Manual The monitor is receiving power (the blue power-on indicator light is on) but is not booting to the usual ‘default’ screen Turn off the universal AC adapter, wait 2-3 minutes and try again. If the problem persists there is likely to be an electronic fault, in which case please contact technical support.
  • Page 40 OxyLite Pro User Manual A sensor displays a toolbar warning triangle Call up the sensor status dialogue by touching the warning triangle on the toolbar to determine the problem (referring to description of sensor status icons in section 3.5). Example sensor warning...

  • Page 41: Table 9: Common Sensor Fault Codes

    Generally speaking, unexpectedly low oxygen readings have a physiological root cause (where used in vivo). For example, the force exerted by the tip of an OxyLite Pro sensor can easily exceed capillary closing pressure. Low pO readings will result if this is the case.

  • Page 42: Simple Sensor Functionality / Calibration Check

    OxyLite (i.e. 200mmHg). With very high pO the OxyLite Pro may no longer report an oxygen value and show an on-screen error state. Another possible source for unexpectedly high oxygen measurements is inappropriate temperature compensation (refer to section 3.8 above).

  • Page 43: Obtaining Technical Assistance

    The monitor should be inspected regularly for signs of wear and tear. The OxyLite Pro monitor is supplied with a 2 year warranty. Please refer to the Terms and Conditions of the Warranty at the end of this user manual for further details.

  • Page 44: Returning The Oxylite Pro Monitor

    Particular attention should be paid to protecting the touch-screen. For a small fee, Oxford Optronix can provide new original shipping cartons and foam inserts that will ensure safe transportation. Failure to pack the OxyLite Pro monitor appropriately for repair or service may result in potentially costly damage to the monitor during transit.

  • Page 45: Technical Specifications

    OxyLite Pro User Manual 6. T ECHNICAL PECIFICATIONS Physical Dimensions 150mm (H) x 310mm (W) x 280mm (D) Weight 4Kg / 9lbs Operating temperature 10 - 30°C Operating humidity 0 – 70% (non-condensing) Power requirements 40W max. Number of sensors supported/...

  • Page 46: Table 10: Oxylite Pro Technical Specifications

    Sensor longevity 48 hours accumulated usage at default sampling rate Analogue data output range 0 – 5V (0 – 200mmHg / 0 – 50ºC) (default) Analogue data output rate Table 10: OxyLite Pro technical specifications Revision 1.2 | May 2015...

  • Page 47: Warranties

    Sensors are warranted for a single use only. Oxford Optronix Ltd. is not liable under this warranty for defects arising from more than one insertion (or use) in vivo, and/or for continuous/accumulated operation in excess of 48 hours at the default sampling rate, and/or for operation in excess of 6 months from factory calibration.
  • Page 48 User Instructions or from any failure to service or maintain the Products in accordance with such instructions. Oxford Optronix Ltd. specifically disclaims any other express or implied warranty, including warranties of merchantability and of fitness for use. To the full extent permitted by law, we shall not be liable in any way whatsoever...

This manual is also suitable for:

Oxylite pro xl

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