INTRODUCTION This manual describes the use of Fluidic Connect 4515 and the installation of the microfluidic chips and the Fluidic Connections. Three different connections will be described in this manual: Fused Silica Connections • fused silica capillaries (375 µm OD, 150 µm ID), 'Nanoport' ferrules and nuts Stainless Steel Connections •...
STEP 1: PARTS CHECK Check the parts list on page 18 to see if all the parts are included. Figure 2.1. All the parts of Fluidic Connect 4515 STEP 2: CHIP PLACEMENT Clean the chip holder and place the chip in the chip holder. The user-friendly design allows the user to push (slide) the chip into the chip holder over a flat surface (see Figure 2.2).
STEP 3: PLACING THE ALIGNMENT NUTS With the Fluidic Connect 4515 two alignment nuts are supplied. The alignment nuts are needed to guarantee good alignment of the chip and should be used after the chip has been placed in the chip holder and before the fluidic connections are made.
Alternatively, the capillary can be cut using a sharp knife. Silica Buffer Coating Figure 2.4. Fused silica Figure 2.5. Cutting the Figure 2.6. Capillary that with a polyimide coating capillary has been cut Fluidic Connect 4515 - User Manual V1.5...
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3. Making fluidic connections At this stage you can place the fittings in the appropriate fluidic ports (see Table 2.1). The fittings can be tightened individually by hand using a special extender tool (Figure 2.8). Fluidic Connect 4515 - User Manual V1.5...
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When sealing with the conical side inside the hole (which is optional), the intensity of the colour of the seal will change by tightening the fittings. The reason for this is the increasing contact area between the sealing rubber and the sidewall of the hole. Fluidic Connect 4515 - User Manual V1.5...
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(Figure 2.12). Tighten comfortably finger- tight. You may use the extender tool to tighten some more, however be sure not to over tighten the ferrule. Fluidic Connect 4515 - User Manual V1.5...
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3. Making fluidic connections Tighten the fitting in the appropriate fluidic ports (see Table 2.1.) by using the extender tool as shown in Figure 2.14. No extreme force should be necessary. Figure 2.14. Tightening the connection Fluidic Connect 4515 - User Manual V1.5...
3 FRAME The Fluidic Connect 4515 is supplied with the Fluidic Support. The support frame can be used for easy inspection of the chip from the bottom side. The chip holder can be placed upside down in the frame (Figure 3.1). Connect the tubing to any auxiliary equipment. The chip holder frame allows the tubing to be guided straight down or to the sides.
The chip is made of glass, tiny cracks in glass can propagate during your tests when the glass is stressed. If this happens, the connections are most likely too tight. Especially with thin bottom chips this is more likely to occur. Fluidic Connect 4515 - User Manual V1.5...
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(torque). With increasing time at high temperature, chemical changes occur. These generally cause an increase in hardness, along with volume and compression set changes. Fluidic Connect 4515 - User Manual V1.5...
TIPS AND TRICKS FOR CONNECTION Please read the tips and tricks before connecting Fluidic Connect 4515. TIGHTENING FORCE Why is the tightening of the connections specified? 1. To create a proper sealing between the ferrule and the chip, the ferrule must be pressed tight against the chip.
3. If the tubing exceeds the ferrule edge, this can cause the problem, see figure 5.2. F e r u l l e a n d tube incorrect assembled Figure 5.2. Assembly of tubing and ferrule Fluidic Connect 4515 - User Manual V1.5...
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