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flexcell HT Cell Seeder User Manual

HT Cell Seeder

™

User Manual

1.2

EVISION

03/25/14

™

Culturing Cells in a Mechanically Active Environment

Flexcell International Corporation  437 Dimmocks Mill Road, Suite 28  Hillsborough, NC 27278

800-728-3714  (919) 732-1591  FAX: (919) 732-5196  www.flexcellint.com



INTERNATIONAL CORPORATION

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Page 1 EVISION 03/25/14 ™ Culturing Cells in a Mechanically Active Environment Flexcell International Corporation  437 Dimmocks Mill Road, Suite 28  Hillsborough, NC 27278 800-728-3714  (919) 732-1591  FAX: (919) 732-5196  www.flexcellint.com  COPYRIGHT © 2012 FLEXCELL INTERNATIONAL CORPORATION...
Page 2 ™ ™ the gasket is placed on top of the HT Cell Seeder . The HT Cell Seeder is only required for seeding cells onto the membrane for a minimal period of time. Afterwards, cell feedings and experiments can be conducted as normal.
Page 3 175 µL. This volume is large enough to allow uniform distribution of the media-cell suspension within the well when dispensing with a 1000 µL micropipette (Figure 4). Also, this volume is ™ small enough to prevent the media-cell suspension from spilling out of the HT Cell Seeder when handling the baseplate. ELL...
Page 4 LEXCELL NTERNATIONAL ORPORATION Figure 4. Suspension volume within the HT Cell Seeder ™ 5. Due to the limited suspension volume, it is advised that the user try to limit the seeding time required for the cells to adhere to the membrane. We recommend a seeding time of at least two hours, but the time that will be required is dependent on the type of cells being used.