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ADInstruments BIO Owner's Manual

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BIO AND NEURO
AMPLIFIERS
Owner's Guide

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  • Page 1 BIO AND NEURO AMPLIFIERS Owner’s Guide...
  • Page 2 This document was, as far as possible, accurate at the time of release. However, changes may have been made to the soft ware and hardware it describes since then. ADInstruments Pty Ltd reserves the right to alter specifications as required. Late-breaking information may be supplied separately.

  • Page 3: Safety Notes

    That is, no product supplied by ADInstruments is intended to be used to diagnose, treat or monitor a subject. Furthermore no product is intended for the prevention, curing or alleviation of disease, injury or handicap.

  • Page 4: Regulatory Symbols

    Quality Management System ISO 9001:2008 ADInstruments manufactures products under a quality system certified as complying with ISO 9001:2008 by an accredited certification body. Regulatory Symbols Amplifiers and signal-conditioners manufactured by ADInstruments that are designed for direct connection to humans and animals are tested to IEC60601-1:2012 (including amendments 1 and 2), and carry one or more of the safety symbols below.

  • Page 5: Safety Standards

    Failure to do so may compromise the inherent safety measures designed into PowerLab equipment. ADInstruments front-ends are only suitable for operation with ADInstruments PowerLabs. Front-ends are suitable for use with any S/, SP/, /20, /25, /30 and /35 series and 15T PowerLabs (FE234 and FE238 only suitable for use with 35 series PowerLabs).
  • Page 6 Several points must be observed for safe operation of the Bio Amp: • All Bio Amp front-ends (except for the FE234 Quad and FE238 Octal Bio Amps) and all PowerLab units with a built-in Bio Amp are supplied with a 3-lead or 5-lead Bio Amp subject cable and lead wire system.

  • Page 7: Cleaning And Sterilization

    Cleaning and Sterilization ADInstruments products may be wiped down with a lint free cloth moistened with industrial methylated spirit. Refer to the manufacturer’s guidelines or the Data Card supplied with transducers and accessories for specific cleaning and sterilizing instructions.
  • Page 8 • Unwanted equipment bearing the Waste Electrical and Electronic Equipment (WEEE) Directive symbol requires separate waste collection. For a product labeled with this symbol, either forward to a recycling center or contact your nearest ADInstruments representative for methods of disposal at the end of its working life. Owner’s Guide...
  • Page 9 All ADInstruments front-ends are designed to be operated under full soft ware control. No knobs, dials, or switches are needed, although some may be provided for reasons of convenience or safety.
  • Page 10 PowerLab. External signals are acquired through the PowerLab analog inputs and amplified before being digitized by the PowerLab. The digitized signal is transmitted to the computer using a fast USB connection. ADInstruments soft ware applications LabChart, LabTutor, LabStation and Lt receive, display, and record the data and your analysis to the computer’s hard disk.
  • Page 11 Connecting to the PowerLab To connect a front-end to the PowerLab, first ensure that the PowerLab is turned off . Failure to do this may damage the PowerLab, the front-end, or both. The BNC cable from the front-end signal output must connect to an analog input on the PowerLab.

  • Page 12: Special Cases

    Multiple Front-ends Multiple separate front-ends can be connected up to a PowerLab. The initial front- end should be connected with the I C cable as in Figure 2–1. The remainder are daisy- chained via I C cables, connecting the I C output of the last connected front-end to the C input of the front-end to be added (Figure 2–2).
  • Page 13 Using ADInstruments Programs Front-ends are designed for use with PowerLabs and ADInstruments programs such as LabChart, LabTutor, LabStation and Lt. The functions of the front-end are combined with those of the PowerLab, and are presented as a single set of soft ware controls in the ADInstruments program.
  • Page 14 Input Amplifier… menu command from the Channel Function pop-up menu in LabChart should be replaced by the <Front-end>... menu command. For example, with a Bio Amp front-end connected, Bio Amp… should appear in the Channel function pop-up menu. Figure 2–3...

  • Page 15: Preventing Problems

    For example, if an ECG was measured with a sampling rate of 100 samples per second (100 Hz) and the Bio Amp had a low-pass filter applied at 50 Hz, the fast-changing sections of the waveform (the QRS complex) may appear smaller and ‘blunted’, while the slower T-wave sections remain relatively...

  • Page 16: Ground Loops

    Similarly, if the high-pass filter was set too high, the amplitude of the T-wave sections may be reduced. The Input Amplifier... dialog allows you to examine ECGs and similar slowly changing waveforms to fine-tune filter settings before recording. Saturation Saturation occurs when the range is set too low for the signal being measured (the amplification, or gain, is too high).
  • Page 17 the mains power. The filtered signal is obtained by subtracting the template from the incoming signal. In comparison with a conventional notch filter, this method produces little waveform distortion. It attenuates harmonics of the mains frequency as well as the 50 or 60 Hz fundamental and therefore eff ectively removes non-sinusoidal interference, such as that commonly caused by fluorescent lights.
  • Page 18 Owner’s Guide...
  • Page 19 • The Bio Amp [FE231], Dual Bio Amp [FE232] and PowerLab units with a built-in Bio Amp are supplied with a 3-lead or 5-lead Bio Amp subject cable and lead wire system. The Octal Bio Amp [FE238] and Quad Bio Amp [FE234] are supplied with unshielded lead wires (1.8m).

  • Page 20: The Front Panel

    The number of amplifiers corresponds to the number of Bio Amp channels. For example, the Dual Bio Amp has two amplifiers with a shared ground connector and input socket, and the Octal Bio Amp has eight diff erential amplifiers with a shared ground connector and separate inputs.

  • Page 21: The Status Indicator

    Quad. The Status Indicator The status indicator light of a Single or Dual Bio Amp is located at the bottom left of the front panel. The status indicator lights of an Octal and Quad Bio Amp are located above the pair of red and black connectors of each input on the front panel.
  • Page 22 C Input and Output The Bio-Amp communicates with the PowerLab via the 9 Pin I2C Input connector. This connector can either be connected directly to a PowerLab or to the ‘I2C Output’ connector of another Front-End. Up to 16 Front Ends can be ‘daisy chained’ in this fashion.
  • Page 23 PowerLab. The sockets are labeled Signal Output on a Single Bio Amp and Output 1 to Output n on a multi-channel Bio Amp. A BNC-to-BNC cable is supplied for each connection. It is recommended that, to avoid confusion, you match the Bio Amp output number with the corresponding PowerLab input connector.
  • Page 24 Dual Bio front panel: a notch in the plug ensures that polarity is correct. The lead wires on the Bio Amp cable plug into the input sockets on the Bio Amp front panel. Only the supplied Bio Amp cable and leads should be used. Other cables may not meet safety requirements.
  • Page 25 The single Bio Amp is supplied with a 3-lead Bio Amp cable with lead wires. The Dual Bio Amp is supplied with a 5-lead Bio Amp cable and separate lead wires; it uses a shared ground signal for its Bio Amp inputs. The supplied cables are of the type used for ECG or EMG studies: Tronomed D-1340 or Tronomed D-1540 cables respectively.
  • Page 26 CH2 Positive (+) CH1 Negative (–) The Octal Bio Amp has a pair of connectors for each of the eight Bio Amp inputs, and a single connector for the shared Ground/reference. The Quad has a pair of connectors for each the four Bio Amp inputs, and a single connector for the shared Ground/reference.

  • Page 27: Signal Display

    LabChart. The Bio Amp dialog The Bio Amp dialog (Figure 4–8 and Figure 4–9) allows soft ware control of the combined input amplifiers and filters in the PowerLab and Bio Amp. The signal present at a channel’s input is displayed in the preview area so that you can see the eff ects of...

  • Page 28: Setting The Range

    The Range pop-up menu lets you select the input range of the channel (combined range of the PowerLab and Bio Amp). Note that range is inversely related to gain (e.g, 100 mV range is the lowest gain and sensitivity setting). Changing the range in the Bio Amp dialog is equivalent to changing it using the arrow in the top right hand corner of the channel in the Chart View Window.
  • Page 29 EOG. Electro-oculogram; a recording of the potential diff erence between the front and back of the eyeball, as projected on to the face. Only supported on Octal and Quad Bio Amps (not supported on Single and Dual Bio Amps) ERG.
  • Page 30 The Bio Amp cable, supplied with the Bio Amp and Dual Bio Amp, plugs into the six- pin input socket on the front panel of the Bio Amp. A notch in the plug ensures that polarity is correct. Only the supplied Bio Amp cable and lead wires should be used as other cables may not meet safety requirements.

  • Page 31: Troubleshooting

    Note: The pin arrangements for the Bio Amp and the Dual Bio Amp are diff erent; the Bio Amp cables are not interchangeable. (The Bio Amp is supplied with a 3-lead Bio Amp cable, whereas the Dual Bio Amp is supplied with a 5-lead Bio Amp cable.)
  • Page 32 • Check the connections for proper contact and try again. If you are using the Dual, Quad or Octal Bio Amps, with the 0.02 Hz high-pass filter, the trace can take a long time to zero by itself.
  • Page 33 , or, if possible, move the subject or equipment outside the area of any interfering field. • At the lowest ranges you may have to shield, shorten, or even replace the Bio Amp leads, since they will tend to act as radio receptors.

  • Page 34: Specifications

    Input impedance: >1 G diff erential, <100 pF (no cable) to isolated ground or 500 pF (supplied shielded Bio Amp cable and leads) to isolated ground Physical Configuration Dimensions (h × w × d): 55 mm ×...
  • Page 35 Dimensions (h × w × d): 70mm x 240mm x 260mm (2.7” x 9.45” x 10.2”) Weight: 2.5 kg (5.5 lb) Power requirements: ~4.5W (Quad), ~7W (Octal) Common specifications for all Bio Amps Input Isolation: 4000 V (50 Hz for 1 minute) Input ranges: ±...

  • Page 36: Control Port

    Input leakage current: < 4 µA @ 240 V, 50 Hz DC tolerance: ± 370 mV Baseline restoration: Automatic or manual Filtering Low-pass filtering: Frequencies soft ware-selectable. 50, 100, 200, 500, 1000, 2000, 5000, 10000 Hz, Off High-pass filtering: Frequencies soft ware-selectable. D.C, 0.0003, 0.001, 0.003, 0.01, 0.03, 0.1, 0.3, 1, 3, 10, 30, 100, 200, 300 Hz Adaptive mains filter:...

  • Page 37: Regulatory Information

    Electromagnetic Compatibility The Bio Amps (the devices) have been tested to comply with the requirements of IEC 60601-1-2. (The relevant basic EMC standards are IEC 61000-4-2, IEC 61000-4-3, IEC 61000-4-6, IEC 61000-4-8, IEC 61000-4-11 and CISPR 11).

  • Page 38: Separation Distances

    Immunity • Mains power quality should be that of a typical commercial or hospital environment. If the user of the device requires continued operation during power mains interruptions, it is recommended that the device be powered from an uninterruptible power supply or a battery. •...
  • Page 39 Chapter 4 Neuro Amp EX The FE285 Neuro Amp EX is one of a family of devices called front-ends, designed to extend the capabilities of the PowerLab system. It diff ers from other front-ends by including an additional piece of hardware, the headstage. The Neuro Amp EX allows the PowerLab system to record extracellular action potentials from single cells or groups of cells.
  • Page 40 However, it is not recommended to use more than one Neuro Amp EX to record from a single subject or to use a Neuro Amp EX with an ADInstruments’ Bio Amp to record from a single subject (this is discussed in more detail in the next section).
  • Page 41 If you are connecting to an earlier model PowerLab with diff erential inputs, only connect the analog output to the positive analog input of the PowerLab. ADInstruments applications do not find a front-end on start up if the negative input is used.

  • Page 42: Software Requirements

    Narrowing the signal bandpass may also reduce the eff ect. Soft ware Requirements The Neuro Amp EX requires the following versions of ADInstruments soft ware applications: • LabChart version 8.1.11 and above Note: the Neuro Amp EX may not operate correctly with earlier versions of these applications.
  • Page 43 Signal amplitude Figure 4–3 The Neuro Amp EX dialog for Windows Range pop-up menu Filtering options Amplitude axis Pause and Scroll buttons Click this button to open the Units Conversion dialog Signal Display The input signal is displayed so you can see the eff ect of changing the settings – no data is recorded while setting things up.

  • Page 44: Types Of Measurement

    signals, respectively. Note that the settings for one filter type may restrict the possible settings for the other. High-Pass Filtering. The High Pass pop-up menu gives the choice of 100 Hz and 300 Hz high-pass filters. The high-pass filter removes frequencies below the selected cut-off frequency and allows high frequencies in the signal to pass.
  • Page 45 terminals are adjacent, while the ground terminal is further away. The positive terminal is distinguished by a slightly recessed surround. Figure 4–4 Signal Input (+) Signal Input (−) Input sockets for the Neuro Amp EX headstage Ground/Reference Connections are made to the Neuro Amp EX front-end using the supplied Neuro Amp EX headstage and connectors (microelectrodes are not supplied).
  • Page 46 6. To help locate the nerve site, nerve stimulating techniques compatible with your institute’s guidelines may be used. Stimulation may be made using the ADInstruments Stimulus Isolator, or another Isolated Stimulator. 7. Changes in signal output in response to nerve stimulation can be observed using the Neuro Amp EX dialog or by recording data using Scope View in LabChart.
  • Page 47 Figure 4–6 The headstage should be securely attached to the subject. The Ground/Reference lead can be attached to a surface electrode using a press stud or snap fitting (Figure 9–10). Figure 4–7 The Ground/ Reference lead wire attached with a press stud Microelectrode needles should be positioned using examination gloves to minimize the risk of infection.
  • Page 48 Figure 4–8 To Ground/ The active Reference microelectrode is electrode inserted over the common peroneal nerve at the fibular head. The reference electrode is ‘Active’ inserted under microelectrode the skin. The ground (Ag/ AgCl) electrode is attached to the surface of the skin with the green Reference lead.

  • Page 49: Technical Aspects

    Technical Aspects The Neuro Amp EX and other ADInstruments front-ends have been designed to integrate fully into the PowerLab system. The Neuro Amp EX is essentially an extension of the PowerLab’s input amplifiers. The amplification and ranges off ered in LabChart result from the combination of both pieces of hardware.
  • Page 50 Neuro Amp EX (Figure 9–14). The pin arrangement ensures that polarity is correct. Only the supplied Neuro Amp EX headstage should be used. Using other headstages invalidates the warranty and may not be as safe. ADInstruments are not responsible for damages incurred if using third party headstages.
  • Page 51 Start up again to see if this has fixed the problem. You are using an early version of LabChart. • Upgrade to the latest version of the soft ware. Contact your ADInstruments representative for information. The BNC or I C cable is faulty.
  • Page 52 PowerLab. If the same problems recur with a second PowerLab, the front-end may be faulty. Contact your ADInstruments representative to arrange for repairs. The trace will not zero properly when adjusting high-pass filtering The Neuro Amp EX is receiving signals at a level that has saturated the input amplifier, resulting in a large off set.
  • Page 53 Specifications Neuro Amp EX Front-end [FE285] Input Connection type: Five-pin Redel connector Configuration: One isolated diff erential channel with isolated ground reference Input impedance: 100 MΩ Safety: Approved to IEC 60601-1 Standard (BF rating) Isolation: 4000 V (50 Hz for 1 minute) Input ranges: ±...
  • Page 54 Complies with IEC 60601-1-2:2014 (tested by EMC Technologies, Sydney, Australia) NOTE: Equipment: Use only with an ADInstruments 35 series PowerLab. PowerLabs The PowerLab must be connected to safety earth via the provide power to power supply cable to ensure electrical safety.

  • Page 55: Electromagnetic Compatibility

    2.8 m This equipment is not intended to be modified or serviced by the user. No user serviceable parts inside. Refer servicing to authorised ADInstruments service centre. ADInstruments reserves the right to alter these specifications at any time. Electromagnetic Compatibility...
  • Page 56 Separation Distances • The device is intended for use in an electromagnetic environment in which radiated RF disturbances are controlled. • Portable and mobile RF communications equipment should be used no closer to any part of the device, including cables, than the recommended separation distance in the table below.

  • Page 57: Warranty

    Warranty Product Purchase and License Agreement This Agreement is between ADInstruments Pty Ltd [‘ADI’] and the purchaser [‘the Purchaser’] of any ADI product or solution — software, hardware or both — and covers all obligations and liabilities on the part of ADI, the Purchaser, and other users of the product. The Purchaser (or any user) accepts the terms of this Agreement by using the product or solution.

  • Page 58: General Limitations

    Hardware Warranty Limitations This warranty applies only to the ADI hardware specified in this document and used under normal operating conditions and within specification. Consumables, electrodes and accessories are not covered by this warranty. Third party equipment may be covered by the third party manufacturer’s warranty.

  • Page 59: Controlling Law And Severability

    sale, installation service or use of its products. EXCEPT FOR THE EXPRESS WARRANTY SET FORTH HEREIN, THE SOLUTION AS WELL AS ALL EQUIPMENT AND SOFTWARE PROVIDED HEREUNDER ARE PROVIDED “AS IS” AND ADI MAKES NO WARRANTY. AS TO ITS USE OR PERFORMANCE. EXCEPT FOR ANY WARRANTY, CONDITION, REPRESENTATION OR TERM THE EXTENT TO WHICH CANNOT BE EXCLUDED OR LIMITED BY APPLICABLE LAW, ADI AND ITS SUPPLIERS MAKE NO WARRANTY, CONDITION, REPRESENTATION, OR TERM (EXPRESS OR IMPLIED, WHETHER BY STATUTE, COMMON LAW, CUSTOM, USAGE OR...

  • Page 60: Technical Support

    LabAuthor are registered trademarks of ADInstruments Pty Ltd. Chart and Scope (application programs), LabTutor Server and LabTutor Online are trademarks of ADInstruments Pty Ltd. The names of specific recording units, such as PowerLab 16/35, are trademarks of ADInstruments Pty Ltd. Windows 8, Windows 7, Windows Vista and .NET Framework are trademarks of Microsoft Corporation.

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