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Shimadzu UV-VIS User Manual
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Shimadzu UV-VIS User Manual

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Shimadzu UV-VIS User's Guide
1)
Push the F4 button on the UV-VIS instrument keypad. This will enable PC
control.
2)
Log into the UV-VIS software with your username and password.
3)
After the UV-Probe software comes up, click on the "Connect" button near the
bottom of the screen to connect with the spectrometer.
Push the F4 Button

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Summary of Contents for Shimadzu UV-VIS

  • Page 1 Shimadzu UV-VIS User’s Guide Push the F4 button on the UV-VIS instrument keypad. This will enable PC control. Push the F4 Button Log into the UV-VIS software with your username and password. After the UV-Probe software comes up, click on the “Connect” button near the...
  • Page 2 Choose what type of experiment mode you need. Spectrum mode is for wavelength scanning over a continuous wide range. Photometric mode is for quantitative analysis, measuring absorbance at single wavelengths. Kinetics mode will collect absorbance data at a particular wavelength versus time. Choose the mode you need by clicking on one of the buttons on the toolbar.

  • Page 3: How To Work In Spectrum Mode

    How to Work in Spectrum Mode After choosing the Spectrum mode of operation, click on the “M” button to set up your acquisition parameters. Enter your wavelength range and scan speed (usually fast), and click on OK. The maximum allowable wavelength range is 1100 nm to 190 nm. It’s good practice to zero the baseline by clicking on the “Baseline”...
  • Page 4 Insert your sample into the sample compartment. If you want to, you can insert a cuvette with blank solution into the reference side. Reference side, towards the back Sample side, towards the front Click on Start to start collecting the spectrum. Please Note: After your spectrum has been collected, the program will prompt you for a filename.
  • Page 5 How to label peaks on your spectrum: Click on the Peak Pick button on the toolbar. Peak Pick button The Peak Pick table will then appear on the left side of the screen. Peak Pick Table Right-click the mouse on the Peak Pick table, and left-click on Properties. You can then change parameters, such as the threshold for labeling peaks, and whether the graph is labeled with the peak table number or the actual absorbance value, and whether peaks or valleys are labeled.
  • Page 6 How to Convert Spectra to ASCII Text Format Click on the “Data Print” button. A Data Print table will appear, listing the raw data points. Click on Data Print Button Data Print Table Appears Right-click the Data Print table, and left-click “Properties”. Make sure that the option “All Data”...
  • Page 7 Click on the File menu, and Save As. Navigate to the folder where you want to save the data. For Save As Type, click on the drop down menu and select Data Print Table (*.txt). Give it a file name, and click on OK. Select “Data Print Table (*.txt)
  • Page 8 Then click on the Disconnect button near the bottom of the screen. Exit from the UV-Probe software. Important! Please go to the UV-VIS instrument keypad, and push the Mode button to return local control to the spectrometer. This allows the instrument to turn off the deuterium lamp, which will make the lamp last longer.

  • Page 9: How To Work In Photometric Mode

    How to Work in Photometric Mode To operate in photometric mode, first click the photometric button on the toolbar. Then click on the “M” button to change your method parameters. Method edit button Photometric button The following pages will come up to set up your experiment. The first page lets you enter which wavelengths you want to use.
  • Page 10 Set concentration units Choose the wavelength to use for calibration Set standard curve fit order The next page lets you enter measurement parameters for your standards. Be sure to click on “Data Acquired By: Instrument”. Be sure Instrument is clicked on.
  • Page 11 The next page lets you set sample measurement parameters. Again, make sure that “Data Acquired By: Instrument” is clicked on. Be sure Instrument is clicked on.
  • Page 12 Next, you need to fill in the information in the Standard Table, and the Sample Table. The Standard table needs concentration information filled in, otherwise the Read Standard button will be grayed out. Be sure to enter the concentrations of your standard solutions.
  • Page 13 Then click on the Disconnect button near the bottom of the screen. Exit from the UV-Probe software. Important! Please go to the UV-VIS instrument keypad, and push the Mode button to return local control to the spectrometer. This allows the instrument to turn off the deuterium lamp, which will make the lamp last longer.