Matrix Impact2 Operator's Manual
  1. Manuals
  2. Brands
  3. Matrix Manuals
  4. Laboratory Equipment
  5. Impact2
  6. Operator's manual

Matrix Impact2 Operator's Manual

Pipettor
Hide thumbs
Table of Contents

Advertisement

Quick Links

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Impact2 and is the answer not in the manual?

Questions and answers

Related Manuals for Matrix Impact2

Summary of Contents for Matrix Impact2

  • Page 2: Table Of Contents

    CONTENTS Congratulations ! ............1 A. INTRODUCTION............2 B. PIPETTOR OVERVIEW..........4 B.1 General Description ..........4 B.2 Keypad..............6 B.3 Display .............. 12 B.4 Specifications ............ 13 B.5 Hazards and Precautions ........14 C. PROGRAMMING AND OPERATION ......15 C.1 Programming the IMPACT........
  • Page 3 CONTENTS (cont’d) E. TROUBLESHOOTING AND MAINTENANCE ....33 E.1 Troubleshooting..........33 E.2 Maintenance............35 E.2.1 Pipettor Disassembly ....... 36 E.2.2 Pipettor Cleaning and Autoclaving....39 E.2.3 Pipettor Reassembly ........ 40 E.3 Recharging Batteries .......... 42 E.4 Replacing Batteries ..........44 F.

  • Page 4: Congratulations

    1 Warranty Card 1 Accuracy/Precision Data Sheet 1 Coupon for Free Calibration Service If you are missing any items, contact your Matrix Technologies representative. Note: Batteries must be installed and recharged before using the . Refer to the following sections in this manual: IMPACT Section E.3, Installing/Replacing Batteries...

  • Page 5: Introduction

    A. INTRODUCTION is lightweight and cordless. It provides the IMPACT convenience of a manual pipettor, with the programming capabilities of an electronic pipettor. It runs on rechargeable batteries and can be used while recharging. The pipettor also has an automatic shutoff feature to extend battery life. uses a "paced dispense"...
  • Page 6 INTRODUCTION IMPACT 2 are available in 8-channel and IMPACT 12-channel formats (see Figure A). An expandable version of the 8-channel format is also available for pipetting samples from test tubes in a rack. It comes in several models to handle fluid volumes from 0.5 µl to 1250 µl.

  • Page 7: Pipettor Overview

    B. PIPETTOR OVERVIEW B.1 General Description Display Keypad Transformer Connector Battery Case Color-coded Ring Pipetting Trigger Ejector Trigger Tip Fittings Figure B.1: Description of Components Keypad. The keypad contains the operation keys that are used in programming the pipetting sequence and volumes. See Section B.2, Keypad.
  • Page 8 Ejector Trigger. The ejector trigger is pressed to eject the pipet tips from the pipettor. Tip Fittings. The tip fittings form an airtight seal with the pipet tips to ensure accuracy while pipetting. Note: To extend instrument life, Matrix Technologies has equipped all pipettors with chemically- IMPACT IMPACT resistant ( ) tip fittings.

  • Page 9: Keypad

    PIPETTOR OVERVIEW B.2 Keypad The keypad contains the operation keys, program keys, and the special function key. Display Fill O n Off Program Operation Keys Disp Prog Keys Keypad Purge Special Enter Function Figure B.2a: IMPACT Keypad Display Fill O n Off Program Operation Keys...
  • Page 10 PIPETTOR OVERVIEW Operation Keys: The On/Off key turns the pipettor on or off. When the [On/Off] pipettor is turned on, it emits a beep tone, flashes " " on the display, then automatically resets the ZERO pistons to zero position. After zeroing, the display shows the first step of the program in the mode.
  • Page 11 PIPETTOR OVERVIEW The current speed setting is always shown at the bottom of the display. These settings can be selected and changed at any time before a program step is performed. The Program key on the enables you to IMPACT [Prog] store up to five separate programs in memory for IMPACT...
  • Page 12 PIPETTOR OVERVIEW Program Keys: The Fill, Dispense, and Mix keys program the [Fill] pipetting operations. By pressing one of these keys, you can enter the programming mode of the [Disp] IMPACT The "Run" prompt disappears from the display and the appropriate operation appears with a flashing [Mix] "Vol"...
  • Page 13 PIPETTOR OVERVIEW The Enter key stores the program in memory. After [Enter] each step is programmed, the Enter key is pressed once to accept the step. After the final step is programmed, the Enter key is pressed twice to denote the end of the program. The pipettor verifies the end of the program with a triple beep tone.
  • Page 14 PIPETTOR OVERVIEW Paced Dispense. When you use the paced dispense feature, pipettor dispenses programmed volume at the specified pace (fast or slow). Immediately after each dispense step, the pipettor emits a single beep tone (if beep tone is on) to prepare you for the next dispense step. See Section C.5, Operating Procedures.

  • Page 15: Display

    PIPETTOR OVERVIEW B.3 Display The pipettor display is a dynamic screen. It displays the current operation, volume prompt (if you are entering a program), pipetting volume, low battery indicator (appears if battery charge is low), beep tone symbol (if the beep tone is on), pipetting speed, and program number ( only).

  • Page 16: Specifications

    PIPETTOR OVERVIEW B.4 Specifications Pipettor Type Model Handle Color IMPACT White Gray IMPACT Handle Specifications Handle Handle Type Volume Color Accuracy* Precision** Size Range Ring (±) (±) 12.50 µl 12-channel 0.50-12.50 Red 2.5%/0.15 µl 2.0%/0.15 µl µl 125.0 µl 12-channel 2.0-125.0 µl Yellow 2.0%/1.0 µl 1.2%/0.8 µl 250.0 µl 12-channel...
  • Page 17 PIPETTOR OVERVIEW 6098 120 V, 60 Hz 6099 Europe 230 V, 50 Hz...

  • Page 18: Hazards And Precautions

    WARNING! Ensure that the power transformer is connected to a power receptacle that provides voltage and current specified by Matrix Technologies. Use of an incompatible power receptacle can cause shock and fire hazard. CAUTION! Use only the power transformer supplied by Matrix Technologies.

  • Page 19: Programming And Operation

    PROGRAMMING AND OPERATION IMPACT 2 Programming the is fast and easy. Pipetting IMPACT steps and volume are entered in the sequence that they will be performed, then the program is saved to memory. This program will stay in memory (even if the pipettor is turned off, or the battery runs low) until you change it.
  • Page 20 PROGRAMMING AND OPERATION 2. Select pipetting operation. Enter the programming mode [Fill] [Mix] by pressing the key. For example, to begin programming with a filling operation, [Fill] press the key. The display shows "Fill" with a flashing "Vol" prompt: Flashing Display Note: The displayed volume defaults to the last volume programmed for the selected operation, or to the default...
  • Page 21 PROGRAMMING AND OPERATION Listed below are some programming considerations for the mix operation: If the program begins a mix cycle from zero position, it will complete the cycle with an automatic blowout, then display " ." Press the trigger to return the pistons to ZERO zero position.
  • Page 22 PROGRAMMING AND OPERATION [Enter] 4. Confirm programming step. Press to confirm the programming step. The display flashes all of the operation keys to prompt you for the next programming step: Flashing Display 5. Select next pipetting operation. Repeat steps 2 to 4 until all programming steps have been entered.

  • Page 23: Programming The Impact 2

    PROGRAMMING AND OPERATION C.2 Programming the IMPACT C.2.1 Program Selection IMPACT 2 To create and store programs in the [Prog] 1. Select the program number by pressing the key, [Vol] then press the key until the desired program number is displayed. [Prog] 2.

  • Page 24: Scratch Pad Mode (Program 0)

    PROGRAMMING AND OPERATION C.2.3 Scratch Pad Mode (Program 0) IMPACT 2 While providing ample program storage, the also offers a “scratch pad” or open programming mode. This mode is identified by “P/0,” which appears in the program section of IMPACT 2 the display (refer to the display on page 12).

  • Page 25: Reviewing The Program

    PROGRAMMING AND OPERATION C.3 Reviewing the Program After you have programmed your pipettor, you may want to review the programming steps: 1. Set pipettor for mode. Ensure that the pipettor is in mode ("Run" appears in the lower left corner of the display).

  • Page 26: Programming Examples

    PROGRAMMING AND OPERATION C.4 Programming Examples Examples of different pipetting sequences are described below. These examples are ideal for use as training exercises. Press the [Vol] keys shown in keycaps , then press the key until the volume in parentheses ( ) is displayed. At the end of the program, a triple beep tone is sounded.

  • Page 27: Serial Dilutions

    PROGRAMMING AND OPERATION C.4.3 Serial Dilutions Example: Transfer 100 µl to the first column of a plate previously filled with reagent. Mix 200 µl for 2 cycles. Transfer 100 µl to the second column and repeat the mix step. Follow this procedure for the rest of the plate.
  • Page 28 PROGRAMMING AND OPERATION C.4.5 Supernatant Collection Example: Aspirate 100 µl from 8 rows of a microtiter plate and discard the total volume. [Fill] (100) [Enter] [Fill] [Enter] [Fill] [Enter] [Fill] [Enter] [Fill] [Enter] [Fill] [Enter] [Fill] [Enter] [Fill] [Enter] [Purge] [Enter] [Enter] This program is performed with the 850 µl handle.

  • Page 29: Operating Procedures

    PROGRAMMING AND OPERATION C.5 Operating Procedures After you have programmed your pipettor, you are ready to begin pipetting. The display should show the first program step, with the "Run" prompt showing in the lower left corner: The following steps describe a basic program for pipetting reagent into a microtiter plate.
  • Page 30 PROGRAMMING AND OPERATION Paced Dispense. Step 4 can also be performed as a paced dispense. Instead of pressing the trigger for each dispense step, press and hold the trigger to automatically dispense each volume. Immediately after each dispense step, the pipettor emits a single beep tone (if the beep tone is on), prompting you to prepare for the next dispense step.

  • Page 31: Calibration

    D. CALIBRATION IMPACT 2 are factory-calibrated for distilled water IMPACT IMPACT 2 at 20°C. For easy calibration, using distilled water, the pipettor features an “ ” mode (see Section D.2, EASY IMPACT 2 Calibrating the ). When pipetting liquids of significantly different specific gravities or temperatures, however, the Calibration IMPACT 2...

  • Page 32: Calibrating The Impact

    CALIBRATION D.1 Calibrating the IMPACT D.1.1 Volume Measurement To measure the actual value of the programmed volume: 1. Fill the programmed volume (V ), then dispense the entire volume into a container on the analytical balance. 2. Read the weight on the analytical balance. Repeat this procedure ten times.

  • Page 33: Pipettor Calibration

    CALIBRATION D.1.2 Pipettor Calibration To calibrate the pipettor: 1. Calculate display volume of the pipettor: D = Programmed Volume = Corrected Actual Volume (corrected for temperature) = Calibration Point (each pipettor handle has its own calibration point, see the table below) = Display Volume Handle Calibration...
  • Page 34 CALIBRATION Example: Calibrate a 1250 µl 8-channel pipettor. Water temperature = 24°C Programmed Volume (V ) = 1000 µl Average of Actual Volume = 990 µl Corrected Actual Volume (V ) = 990 x 1.004 = 994 µl Calibration Point (C ) = 1000 = 1000 µl x 1000 994 µl...

  • Page 35: Calibrating The Impact 2

    CALIBRATION D.2 Calibrating the IMPACT users can calibrate their pipettors with distilled water IMPACT using the “ ” mode or with other liquids using the original EASY “ ” mode (displayed as “ ” on the CALC IMPACT D.2.1 “EASY” CAL Mode users can quickly calibrate their pipettor by using the IMPACT unique “...

  • Page 36: Calc" Mode

    CALIBRATION 5. Verify this new calibration setting by repeating Steps 1 and 2. D.2.2 “CALC” Mode To calibrate the pipettor for liquids other than water, use IMPACT the original mode (displayed as “ ” on the CALC IMPACT Measure the programmed volume (see Section D.1.1, Volume Measurement 2.

  • Page 37: Recommendations For Accurate Pipetting

    CALIBRATION resets factory setting (see Table D.1.2, Calibration Specifications, for the factory calibration steps). D.5 Recommendations for Accurate Pipetting pipettors are factory calibrated to deliver IMPACT IMPACT accurate volumes with distilled water at 20°C. By using the calibration procedures described in Sections D.1, Calibrating the , and D.2, Calibrating the , you can pipet liquids IMPACT...

  • Page 38: Troubleshooting And Maintenance

    Listed below are possible procedural and system problems, as well as recommended solutions. If you cannot resolve the problem, call Matrix Technologies, Technical Service Depart-ment. No power to the pipettor. Batteries are incorrectly installed. Recheck the orientation of the batteries, they should match the diagram in the battery case.

  • Page 39: Maintenance

    Caustic cleaning solutions can damage the keypad. Note: For autoclaving specific portions of the pipettor refer to the Autoclaving Procedures, or call Matrix Technologies, Technical Service Department, for instructions. Note: Avoid prolonged exposure to ultraviolet light. The handle...
  • Page 40 Under normal use, the batteries can be recharged approximately 400 times. When they can no longer hold their charge, the batteries must be replaced. Replacement batteries can be purchased through Matrix Technologies. The following procedure describes installation, or replacement, of the batteries: Note: If you are replacing batteries, ensure that the pipettor is turned off before removing the battery case.
  • Page 41 TROUBLESHOOTING AND MAINTENANCE 2. Remove batteries. Press a pipet tip against either battery through the slot in the bottom of the battery case to remove the batteries. Battery Case Pipet Tip (bottom) Batteries (behind slot) Figure E.3b: Removing Batteries 3. Install new batteries. Insert new batteries by placing the positive end into the battery case first, then the negative end.

  • Page 42: Recharging Batteries

    Note: Spare batteries and external battery chargers can be ordered through Matrix Technologies. Battery charge is low when the battery symbol – + appears on the display. The pipettor will continue to function, but should be recharged at the earliest convenient time.

  • Page 43: Additional Pipetting Examples

    F. APPENDICES F.1 Additional Pipetting Examples F.1.1 Sample Transfers Example #1: Transfer 100 µl and mix 200 µl for 2 cycles immediately after transfer. [Fill] (100) [Enter] [Purge] [Enter] [Mix] (200) [Mix] [Enter] [Enter] [Enter] When a purge is programmed before a mix cycle, the pipet tips are emptied and mixing occurs automatically without pressing the trigger.

  • Page 44: Incremental Pipetting

    APPENDICES F.1.2 Incremental Pipetting Example: Fill 250 µl and dispense 45 µl in the first column of the microtiter plate. Reduce each subsequent column by 5 µl for a total of 8 columns. Discard the remainder. [Fill] (250) [Enter] [Disp] (25) [Enter] [Disp]...

  • Page 45: Simple Dilutions

    APPENDICES F.1.4 Simple Dilutions Example: Prepare a 1:21 dilution with a 10 µl sample volume. Dispense the total volume into a column and mix immediately. [Fill] (200) [Enter] [Fill] [Enter] (20) [Fill] [Enter] (10) [Purge] [Enter] [Mix] (150) [Enter] [Enter] This program contains an air-gap (20 ul) and mix step at the end.

  • Page 46: Customer Service

    IMPACT by Matrix Technologies Corp. to be free of defects in materials and workmanship for a period of twelve (12) months from the date of purchase, providing that it has been operated according to the...
  • Page 47 AUTOCLAVING PROCEDURES for IMPACT Pipettors MATRIX TECHNOLOGIES CORPORATION...
  • Page 48 Matrix Technologies for repair. In cases where pipettors have been exposed to biohazardous materials, autoclaving internal components may be required. Matrix Technologies provides the following Autoclave Kit: Autoclave Kit (Part No. 9997) 12 replacement cross tubes 1 wooden applicator...
  • Page 49 PIPETTOR DISASSEMBLY NOTE: Follow standard laboratory practices for using or servicing equipment that is exposed to biohazardous material. 1. Remove the color ring by gently prying loose one of the edges near the handle trigger. 2. Use a Phillips screwdriver to remove all three housing screws. 3.
  • Page 50 PIPETTOR DISASSEMBLY Item Description Item Description keypad tip fitting main board console cover flex circuit console display battery box batteries driver board main housing motor trigger hinge pin color ring handle trigger motor coupling handle trigger spring Autoclaving...
  • Page 51 PIPETTOR DISASSEMBLY 4. Remove the plastic ejector trigger with spring and slide the aluminum ejector comb off of the tip fittings (see Figure 3). Ejector Trigger Ejector Comb Figure 3: Removing Ejector Trigger and Ejector Comb 5. Remove the piston/cylinder assembly by pulling the motor screw down from the motor shaft coupling and out of the main housing.
  • Page 52 AUTOCLAVING 1. Remove the piston assembly from the cylinder. 2. Remove and discard the cross tubes into a biohazardous container. 3. Remove the o-rings (only if necessary, see section “Replacing O-rings”) and discard them into a biohazardous container. 4. Place all components (see Figure 5) into the autoclave and autoclave at 121 °C for 20 minutes.
  • Page 53 PIPETTOR REASSEMBLY After autoclaving, and once all of the components have completely dried, the unit is ready for relubrication and reassembly. Cross Tube Replacement Refer to the following table for proper connection of cross tubes from the tip fittings to the cylinder positions. Tip Fittings Manifold Assembly 850, 250, 125, and 12.5 ul Cylinder...
  • Page 54 In this case, the o -rings should be removed prior to autoclaving. Spare o-ring packs are available through Matrix Technologies for all pipettor volumes. 1. Remove old o-rings • 1250 ul, 850 ul, 250 ul Pipettors: O-rings can be removed from the pistons by squeezing one side of the o -ring and lifting it out of the o-ring groove on the piston.
  • Page 55 PIPETTOR REASSEMBLY Relubrication • 1250 ul, 850 ul, 250 ul Pipettors: Use the wooden applicator and O-ring l u bricant supplied in the Autoclave Kit to apply a thin layer of lubrication approximately 0.5 inches (approx. 1.3 cm) down into each piston hole that requires it. Spread the lubricant evenly along the inner wall of each piston by using a circular motion with the wooden applicator (see Figure 7).
  • Page 56 PIPETTOR REASSEMBLY Reassembly 1. Reinsert the piston assembly into the lubricated cylinder. Ensure that the motor screw engages into the hole in the center of the cylinder (see Figure 9). NOTE: To correctly orient the piston and cylinder assembly, ensure that the lot number of the cylinder is facing up and the painted section of the piston backplate is facing down.
  • Page 57 PIPETTOR REASSEMBLY Slowly spin the motor screw until the flat end of the motor screw is fully engaged into the motor coupling hole (see Figure 10). The cylinder can now be seated between the plastic ribs in the main housing. Motor Coupling Motor Screw Piston Backplate...
  • Page 58 PIPETTOR REASSEMBLY To keep the tubing free from ejector contact, rotate the manifold plate tubing complete turn counterclockwise (see Figure 11). Insert the manifold assembly into the main housing support ribs. Figure 11: Rotating Manifold Assembly 4. Slide the ejector comb over the tip fittings with the slots on the back of the comb exposed (see Figure 12).
  • Page 59 PIPETTOR REASSEMBLY 5. Hold the ejector comb in place while inserting the ejector trigger (see Figure 13). Place the end of the ejector spring against the plastic stop and compress the spring while positioning the ejector trigger over the aluminum ejector comb (the ribs on the ejector trigger should mate with the slots on the aluminum ejector comb).
  • Page 61 STORED PROGRAMS Use the following worksheet to determine the pipetting steps for each program number, then keep it handy as a reference. The page is perforated so that it can be removed and easily viewed while pipetting. Date Program Program Description ç...
  • Page 62 Figure B Figure C The Matrix Impact and Impact Equalizer allows the Tip to Tip spacing of the pipettor to change. This allows pipeting into or between vessels of varying center to center configurations. The set button(s), indicator and control rod all work together to change the distance between the tips (see figure A).

This manual is also suitable for:

Impact

Table of Contents