Table of Contents
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Strathkelvin 782 2-Channel Oxygen System version 1.0
CONTENTS
1
SAFETY AND TECHNICAL INFORMATION....................................................... 1
2
THE 782 SYSTEM...................................................................................................... 2
2.1
The 782 System...................................................................................................................................2
2.2
Minimum System Requirements .......................................................................................................2
2.3
The 782 Meter........................................................................................................................................2
2.4
1302 Microcathode Oxygen Electrodes.............................................................................................4
2.5
Computer Software................................................................................................................................5
2.6
Product Support....................................................................................................................................5
2.7
Uses of the 782 System.........................................................................................................................5
3
GETTING STARTED................................................................................................. 7
3.1
Software Installation.............................................................................................................................7
3.2
Setting up the 782 Meter .....................................................................................................................7
3.3
Operation .........................................................................................................................................10
4
782 OXYGEN METER..............................................................................................11
4.1
Introduction ..........................................................................................................................................11
4.2
Setup......................................................................................................................................................11
4.3
Calibrating your Electrodes ..............................................................................................................13
4.4
Data Logging ........................................................................................................................................15
4.5
Up loading Stored Data from 782 Meter..........................................................................................17
5
782 SYSTEM SOFTWARE .....................................................................................24
Strathkelvin Instruments } 782 User's Guide
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Table of Contents
Summary of Contents for Strathkelvin 782
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Page 1: Table Of Contents
Strathkelvin 782 2-Channel Oxygen System version 1.0 CONTENTS SAFETY AND TECHNICAL INFORMATION............1 THE 782 SYSTEM...................... 2 The 782 System...........................2 Minimum System Requirements .......................2 The 782 Meter............................2 1302 Microcathode Oxygen Electrodes.....................4 Computer Software..........................5 Product Support............................5 Uses of the 782 System.........................5 GETTING STARTED.................... - Page 2 Adding Event Markers to the Recording Area................40 5.16 Ending Recording ...........................40 ANALYSIS OF DATA....................42 Introduction ............................42 Accessing the Analysis Screen......................42 The Analysis Screen...........................43 Analysing Closed Cell Respirometry Files ..................46 Analysing Flow-through Respirometry Files.................47 Monitoring experiments........................48 Strathkelvin Instruments } 782 User's Guide...
- Page 3 Appendix 4: Troubleshooting ......................54 Appendix 5: Rechloriding electrode anode ..................56 Appendix 6: Calibration solutions ....................57 Appendix 7: Oxygen solubility tables....................58 Appendix 8: Conversion units ......................59 Appendix 9: Electrolyte solution.......................59 8.10 Appendix 10: References ......................60 Strathkelvin Instruments } 782 User's Guide...
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Page 4: Safety And Technical Information
(Permitted Performance Class A, Electromagnetic Compatibility Directive.) The 782 meter conforms to the requirements of the Low Voltage Directive 73/23/EEC as per generic standard EN61010-1, part 1. SPECIFICATION: Inputs: 2 oxygen electrode connectors, bias voltage 650mV, maximum input current 2.6 nA. -
Page 5: The 782 System
Minimum System Requirements The 782 system is supplied as a meter, USB connecting cable, software and instruction manual. In addition, you will need up to 2 oxygen electrodes, an electrode service kit, and respirometer chambers available from Strathkelvin Instruments. - Page 6 These only function when the meter is not connected to a computer running the Strathkelvin software. The display also shows the oxygen concentration values with the units (selected in setup) plus difference (if selected in setup) and time and date (if selected in setup). Strathkelvin Instruments } 782 User's Guide...
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Page 7: 1302 Microcathode Oxygen Electrodes
1302 Microcathode Oxygen Electrodes These electrodes, which are in use worldwide with Strathkelvin's 781, 782 and 928 instruments, have the advantage of small size, high precision, and the ability to operate in either stirred or unstirred media. They are supplied with polypropylene membranes, but for fast speed of response, they can be fitted with FEP membranes. -
Page 8: Computer Software
For any other problems contact Strathkelvin Instruments directly by e-mail: info@strathkelvin.com Uses of the 782 System The 782 has been designed to meet the need for replication of dissolved oxygen measurement, with provision for up to 2 oxygen electrodes. The main uses of the system are as follows:... - Page 9 In many situations there is simply a requirement for logging of oxygen levels, as in the outflow of blood or saline from organ preparations. The 782 is ideally suited to log data from the oxygen electrodes in such experiments. Strathkelvin Instruments } 782 User's Guide...
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Page 10: Getting Started
Connect the USB cable. On the PC, Windows will detect the connection and show this dialog to initiate the installation of the appropriate USB driver: Strathkelvin Instruments } 782 User's Guide... - Page 11 Click Next to display this dialog: Select Search for the best driver… as shown and click Next to show: Strathkelvin Instruments } 782 User's Guide...
- Page 12 Tick CD-ROM drive only and click Next to show: Click Next to install the driver. A final dialog announcing that Windows has finished installing the driver should appear. Click Finish on this dialog and remove the CDROM from your computer. Strathkelvin Instruments } 782 User's Guide...
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Page 13: Operation
Operation Always switch the meter on before starting the 782 computer program. When first switched on, the meter initialises its internal program and then displays the oxygen concentrations using the settings stored when it was switched off. The front buttons will be operational. -
Page 14: 782 Oxygen Meter
If the meter will always be used with a computer then the setup and running procedures will be carried out using the 782 System Software which is described in Chapter 5. Setup When the meter is switched on its display will look similar to that below. - Page 15 Time/date display: ON/OFF Note: The Time/date display is set to Windows time/date settings every time the meter is connected to the PC and the Strathkelvin software is run. Data Logging: ON/OFF This enables a facility for saving oxygen measurements to the memory of the meter (see section 4.4)
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Page 16: Calibrating Your Electrodes
(see Appendix 8.7), if using concentration units. The high point value can be changed by using the q button to highlight the High point value: and then press Change. The meter will then display the following: Strathkelvin Instruments } 782 User's Guide... - Page 17 CANCEL the calibration at this stage. 5. You will then be asked to wait while the electrode(s) stabilise. At the end of this one minute waiting period, the display below will appear. Strathkelvin Instruments } 782 User's Guide...
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Page 18: Data Logging
(amount of free memory). This will be displayed on the lower right- hand side of the display under the heading Free. Strathkelvin Instruments } 782 User's Guide... - Page 19 The display will list the number of readings that can be saved in the lower right hand side under the heading Free. Before any readings have been saved it will show 3272 when 2 Strathkelvin Instruments } 782 User's Guide...
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Page 20: Up Loading Stored Data From 782 Meter
Uploading Stored Data from the 782 Meter Connect the meter to the PC using the USB cable. Start the Strathkelvin software and press the Upload data button will cause the Upload data from meter dialog to appear. If no data is available, the dialog will display 'No data stored'. If data is loaded from the meter... - Page 21 Select the Type of experiment by highlighting one of the three options. Click Set titles etc to display the dialog which allows the entry of a title and description of the experiment. The text will be saved with the data file (recording). Strathkelvin Instruments } 782 User's Guide...
- Page 22 The units should be selected from the list box below. Water Vol. Enter the volume of water or solution in each chamber. The units should be selected from the list box below. Strathkelvin Instruments } 782 User's Guide...
- Page 23 This box will appear on the dialog if you have measured in units of % saturation. Enter the oxygen concentration of water or solution which is 100 % saturated with air. You can obtain these values from published tables (see Appendix 8.7). Strathkelvin Instruments } 782 User's Guide...
- Page 24 (A, B) for each respirometer chamber. These chamber identifiers will be used subsequently in the Analysis part of the program. If you are using two electrodes it is important to check that they are connected to the 782 inputs so that their numbers match the positions shown.
- Page 25 The time of each sample is also saved. Because the time intervals between samples are i r regular, this data cannot be used by the Analyse part of the program and must be copied to an external spreadsheet (eg. Excel) for analysis and plotting. Strathkelvin Instruments } 782 User's Guide...
- Page 26 Both versions of the dialog contain an Erase data button which empties the data memory of the meter. Caution: Once erased the data cannot be resurrected, so use the Erase data button only after you have uploaded and saved the data on the PC. Strathkelvin Instruments } 782 User's Guide...
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Page 27: 782 System Software
Switch the computer on. Switch the meter on using the power button on the front panel. The LCD displays the current electrode readings. On the desktop of the PC double click the 782 icon. This brings up the sign on dialog, offering three option buttons. Experiment to prepare and run an experiment. -
Page 28: The Setup Screen
If you have forgotten to switch on the meter, you will receive a warning message: Communication with the 782 meter failed. If this happens, press the power button (see Section 2.3). - Page 29 Temperature and oxygen solubility… allows you to enter the temperature of the next calibration and of subsequent experiments and to calculate or enter the oxygen solubility. Calibrate… displays a dialog used to calibrate the oxygen electrode(s). Strathkelvin Instruments } 782 User's Guide...
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Page 30: Preparing The Experiment
Alternatively this can be done after the experimental run, but before analysis. Call up the 782 program by double clicking the icon on the desktop. On the Sign On screen click Experiment to bring up the Setup screen. -
Page 31: Basic Setup
Respiration rate units. If you want to express the respiration rate in units other than those shown, open the list box and select the required units. (If you are monitoring O , this box will be greyed out.) You must select Recording units first. Strathkelvin Instruments } 782 User's Guide... -
Page 32: Setting Temperature And Oxygen Solubility
This displays the dialog below. You must use this dialog before Calibration, since the High point for calibration will be calculated here. If you have elected to measure oxygen in concentration units or as %satn in the Basic setup dialog, the dialog appears thus: Strathkelvin Instruments } 782 User's Guide... - Page 33 Calculate solubility. No High point value will be entered into the Calibrate dialog, which will instead show the last one used. Strathkelvin Instruments } 782 User's Guide...
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Page 34: Calibration
100% if you are working in %satn units. b) If you have calculated the oxygen concentration at air saturation automatically using the Oxygen solubility dialog, the resulting value will be displayed here. Strathkelvin Instruments } 782 User's Guide... -
Page 35: Setting Experimental Values
Setting Experimental Values Select Setup experiment from the menu bar, and then select Set experiment values. The dialog which is displayed depends on whether you have previously chosen Closed cell, Flow-through respirometry or Monitor only. Strathkelvin Instruments } 782 User's Guide... - Page 36 Very often it happens that the weight of the organism is not measured until after the respiration run is over. To accommodate this situation, you can enter the values during Analysis. Water volume Strathkelvin Instruments } 782 User's Guide...
- Page 37 Enter here the title of the experiment. This appears on the recording screen and in the Report. Label You may record information about the material in each respirometer chamber. Up to 15 characters are available in each box. Strathkelvin Instruments } 782 User's Guide...
- Page 38 Enter here any further information about the experiment which you wish to appear on the Report. Click on OK when complete. 3. Monitoring oxygen levels If you selected Monitor only, you will now see the following dialog: Strathkelvin Instruments } 782 User's Guide...
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Page 39: Setting Recording Screen Parameters
Enter here any further information about the experiment which you wish to appear on the Report. Click on OK when complete. Setting Recording Screen Parameters Click the Display menu and then the Set scales option to bring up this dialog: Strathkelvin Instruments } 782 User's Guide... -
Page 40: Printing Screen Traces
Details of the experiment, from information provided during Setup, together with information on markers used during Recording, are printed when the recording is saved at the end of the run. Strathkelvin Instruments } 782 User's Guide... -
Page 41: Setting Trace Colors
You can now turn your attention to the respirometers. Re-insert the electrodes if they have been removed for calibration. Add the water, followed by the respiring preparation, and seal the respirometers (for closed chamber) or start the water flow (for flow-through). Click the Start button. Strathkelvin Instruments } 782 User's Guide... -
Page 42: Recording An Experiment
Setup. At the same time a vertical line is applied to the screen at the right hand side and the oxygen value traces scroll from right to left at a rate which you determined in the Set Scales dialog. Strathkelvin Instruments } 782 User's Guide... -
Page 43: Adding Event Markers To The Recording Area
15. 5.16 Ending Recording At the end of the experiment, click Stop. Another vertical line will appear and you now have the opportunity to save the respiration run data in a datafile: Strathkelvin Instruments } 782 User's Guide... - Page 44 You now have two options: 1. To proceed immediately to analysis of the recorded data. 2. To exit the program and analyse the recording later. Both of these options are available from the Setup screen File menu. Strathkelvin Instruments } 782 User's Guide...
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Page 45: Analysis Of Data
Analysis screen. 2) At some other stage, after exiting the program or uploading data from the 782 meter memory. Very often you may wish to record several experiments successively and then return to the analysis perhaps on another day. -
Page 46: The Analysis Screen
The Analysis Screen The Analysis screen comprises two separate tabbed pages. The one that you now see, with a Traces tab, and the Report page, beneath it and accessed by the Report tab. Strathkelvin Instruments } 782 User's Guide... - Page 47 (closed cell) or biomass or flow rate (flow-through) if these were not entered in Setup. Comments… allows you to enter comments on the experiment. Trace Colors ... allows you to change the trace colors. Strathkelvin Instruments } 782 User's Guide...
- Page 48 Calc. X-axis controls Scroll slider bar enables you to scroll along the trace to find the sections of trace which you want to analyse. Strathkelvin Instruments } 782 User's Guide...
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Page 49: Analysing Closed Cell Respirometry Files
For example, click button 1 on the toolbar. The red number 1 trace disappears. Click button 1 again to restore it. Now click Setup Report. This brings up the Select calculation and report format dialog: Strathkelvin Instruments } 782 User's Guide... -
Page 50: Analysing Flow-Through Respirometry Files
For example, click button 1 on the toolbar. The red number 1 trace disappears. Click button 1 again to restore it. Now click Setup Report. This brings up the Select calculation and report format dialog: Strathkelvin Instruments } 782 User's Guide... -
Page 51: Monitoring Experiments
Then use the Scroll scrollbar to bring the second half of the experiment on to the screen. Repeat at say 13 minutes and 18 minutes. Then open the ‘ R eport’ window to view the selected values. Strathkelvin Instruments } 782 User's Guide... -
Page 52: Dealing With Reports
The Save icon button enables you to save the results directly to the \Strathk\data folder as a spreadsheet file (normally Excel format, but other formats can be chosen). To exit click File and choose Close option. This closes the Analyse screen and its Report page. Strathkelvin Instruments } 782 User's Guide... -
Page 53: Appendices
Appendices Appendix 1: Error displays Error displays on the 782 meter ELECTRODE FAULT This displays when the electrode output is too high for the meter. With the 1302 electrode this indicates a fault, which is normally due to either a short circuit in the electrode cable or connectors, or due to a damaged membrane. - Page 54 Stand the electrode jacket with its broad end on the bench. Now take an 'O' ring and tension it by sliding it on to the conical applicator. Stand the applicator on the bench and carefully push the 'O' ring down to the farthest edge. Strathkelvin Instruments } 782 User's Guide...
- Page 55 If it is to be stored for longer than that, remove the jacket and empty out the electrolyte. Rinse the inside of the jacket and electrode with distilled water, dry and refit the jacket and store dry in the electrode box. Strathkelvin Instruments } 782 User's Guide...
- Page 56 The associated pressure change could cause the membrane to bulge and the response time of the electrode would increase. If an air bubble is present, it is best to top up the electrolyte level, before raising the temperature. Strathkelvin Instruments } 782 User's Guide...
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Page 57: Appendix 3: Checking Electrodes
If the problem persists, it may indicate an internal short circuit within the electrode. This cannot be repaired. Return the electrode to your distributor or to Strathkelvin Instruments for verification. Very high output This may indicate that you have an air bubble trapped between the cathode and the membrane. - Page 58 Add more electrolyte to the jacket and screw on tight again. Clean the cathode – Appendix, Section 8.2. Rechloride the anode – Appendix, Section 8.5. Strathkelvin Instruments } 782 User's Guide...
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Page 59: Appendix 5: Rechloriding Electrode Anode
Strathkelvin Instruments. We will do our best to service and repair the electrode although where there is excessive dark current, repair is sometimes not possible. -
Page 60: Appendix 6: Calibration Solutions
Some early literature suggested the use of sodium dithionate solution. Do not use this, since its breakdown products can impair the functioning of the cathode. Rinse all traces of the sulphite from the electrode after zero calibration. Strathkelvin Instruments } 782 User's Guide... -
Page 61: Appendix 7: Oxygen Solubility Tables
From reference (3). Mitochondrial medium: Concentration of oxygen at 100% saturation with air. Temperature Concentration Concentration (air saturation) (air saturation) °C µmol O µg atoms O 0.575 0.510 0.474 0.445 0.410 0.398 0.380 From reference (3). Strathkelvin Instruments } 782 User's Guide... -
Page 62: Appendix 8: Conversion Units
Finally drop in a small crystal of thymol, to inhibit fungal and bacterial growth. Shake vigorously and leave for 12 hours for the thymol to go into solution. Filter into a stoppered bottle. Strathkelvin Instruments } 782 User's Guide... -
Page 63: Appendix 10: References
Research 25, 140 - 147. Umbreit, W.W. et al (1964). Manometric techniques (4th Edn), Burgess Publ Co. (Contains a wealth of information on tissue metabolism, using Warburg and similar techniques.) 14/03/03 782 Manual.doc Strathkelvin Instruments } 782 User's Guide...
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