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Waters XBridge Peptide BEH C18 Care And Use Manual

130a and 300a columns
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[ CARE AND USE MANUAL ]
XBridge Peptide BEH C
CONTENTS
I.
II.
III.
IV.
V.
VI.
VII.
, 130Å and 300Å Columns
18
VIII.
IX.
X.
Thank you for choosing a Waters® XBridge® Peptide BEH
C
130Å and 300Å packing materials were designed to provide
excellent peak shape, high efficiency, and excellent stability.
The XBridge Peptide BEH C
are manufactured in a cGMP, ISO 9002 certified plant using
ultra pure reagent. Each batch of XBridge Peptide BEH C
Column material has been qualified with a peptide separation,
and the results are held to narrow specification ranges to
assure excellent, reproducible performance for peptide
separations. Every column is tested and a Performance Test
Chromatogram, along with a Certification of Acceptance, are
provided with each column.
on 2.1 mm I.D. Column Performance
CAUTIONARY NOTE
, 130Å or 300Å Column. The XBridge Peptide BEH C
18
, 130Å and 300Å packing materials
18
,
18
18
1

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Summary of Contents for Waters XBridge Peptide BEH C18

  • Page 1: Table Of Contents

    PROTEIN DIGEST c. Solvents d. Pressure CAUTIONARY NOTE e. Temperature Thank you for choosing a Waters® XBridge® Peptide BEH III. SCALING UP/DOWN ISOCRATIC METHODS , 130Å or 300Å Column. The XBridge Peptide BEH C TROUBLESHOOTING 130Å and 300Å packing materials were designed to provide excellent peak shape, high efficiency, and excellent stability.

  • Page 2: Getting Started

    1. Perform an efficiency test on the column before using it peptide column being installed. See “Scaling Up/Down Isocratic Separations” in the desired application. Waters recommends using a section for calculating flow rates when changing column I.D. and/or length. suitable solute mixture, as found in the “Performance Test See “Connecting the Column to the HPLC System”...

  • Page 3: Column Use

    Operating pH limits 1. Sample impurities often contribute to column The recommended operating pH range for XBridge Peptide contamination. One option to avoid this is to use Waters BEH C , 130Å and 300Å Columns is 1 to 12. A listing of Oasis®...
  • Page 4 [ CARE AND USE MANUAL ] Table 2. Buffer Recommendations for Using XBridge Peptide BEH C , 130Å and 300Å Columns from pH 1 to 12 Buffer Volatility Used for Additive/Buffer pK a Comments range (±1 pH unit) mass spec Ion pair additive, can suppress MS –...

  • Page 5: Solvents

    “HPLC Columns 2.1 mm 18,000 psi (1034 bar) Theory, Technology and Practice”, U.D. Neue, (Wiley-VCH, 1997), 3.0 mm 18,000 psi (1034 bar) the Waters HPLC Troubleshooting Guide p/n: 720000181EN) or 4.6 mm 9000 psi (620 bar) visit www.waters.com for seminar information.

  • Page 6: Storage

    The choice of appropriate column connectors and system tubing is discussed in detail below. .130” .090” Waters Ferrule Setting Parker Ferrule Setting Figure 1. Waters and Parker Ferrule Types. XBridge Peptide BEH C , 130Å and 300Å Columns...
  • Page 7 Waters style end fitting (Figure 3). tools (Figure 5). Note: A void appears if tubing with a Parker ferrule is connected to a Waters style column. Void Figure 3.

  • Page 8: Measuring System Band Spreading Volume

    Unique design separates tube-holding function from ■ ■ parabens; p/n: WAT034544). sealing function Inject 2 to 5 µL of this solution. Table 5. Waters Part Numbers for Measure the peak width at 4.4% of peak height SLIPFREE Connectors (5-sigma method): SLIPFREE Type Tubing Internal Diameter 5-sigma band spreading (µL) =...

  • Page 9: Ecord Intelligent Chip Technology

    (t ) (half the vertical distance between the initial and final isocratic segments Waters eCord Intelligent Chip. as shown in Figure 8). At the time of manufacture, tracking and quality control information will be downloaded to the eCord. Storing this information on the chip will eliminate the need for a paper Certificate of Analysis.

  • Page 10: Additional Information

    [ CARE AND USE MANUAL ] c. Manufacturing Information of first injection, date of last injection, maximum pressure, and temperature. The information also details the column history by sample set including date started, sample set name, user name, system name, number of injections in the sample set, number of samples in the sample set, maximum pressure, and temperature in the sample set;...

  • Page 11: Non-Optimized Vs. Optimized Lc-Ms/Ms System: System Modification Recommendations

    34 Maple Street Milford, MA 01757 U.S.A. Waters, The Science of What’s Possible, Oasis, Sep-Pak, XBridge, Empower, and MassLynx are registered T: 1 508 478 2000 trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

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