Harvard Apparatus CMA 31 User Manual

CMA 31 Linear Microdialysis Probe User's Manual

TECHNICAL INFORMATION

Membrane

Material
Molecular Cut-Off
Outer Diameter
Length

Probe Shaft

Material
Length Inlet Tubing
Length Outlet Tubing
Total Length (tubing + membrane)
Outer Diameter
Double Tubing Outer Diameter
Double Tubing Length
The probe can be sterilized in its inner package with ethylene oxide.

Cutaneous Implantation, Anesthetized Animal

For CMA 31 Linear Microdialysis Probe: To avoid ultra filtration over the membrane
the probe should be used with a Perfusion Fluid consisting of a Ringer solution
containing 30g Dextran 60/1000mL.
1. Anesthetize the animal. Be sure to keep the animal's body temperature normal
during the surgical procedure and the study period by using a CMA 450
Temperature Controller.
2. Clean and shave the animal.
3. Mark the skin with a felt tip marker where the introducer needle should go in
and exit, leaving at least an 18 mm window if drug transport is studied. Tunnel
the introducing needle at the appropriate depth required for the experiment.
4. Load the CMA pump with a Perfusion Fluid-filled syringe (either a Luer lock
single use syringe or a glass syringe with a fixed needle). Make sure there are
no air bubbles.
5. Connect the inlet Luer lock connector to the single use syringe or cut off the
Luer lock connector from the probe inlet with a sharp cut, and connect it to
the glass syringe needle via a
6. Start the pump at 5 µL/min. Confirm that liquid is flowing from the outlet of
the probe. Maintain this flushing rate for approximately 2-3 min to remove air
bubbles.
7. Reduce the flow rate to operational rates, usually 1-3 µL/min.
8. Remove the protective tubing from the probe. Slide the probe outlet tubing
through the needle end of the introducer carefully so the membrane sits in the
middle of the introducer. Make sure not to kink the tubing.
9. Check that the flow rate is the same as before the insertion. Hold the probe in
place against the skin while carefully withdrawing the introducer needle.
10. Secure the probe position with surgical tape or surgical glue.
11. Wait at least 30 min for stabilization before starting the sample collection.
Polyethersulfone (PES)
55,000 Daltons
0.26 mm
10 mm
Polyimide
350 mm
100 mm
460 mm
0.19 mm
0.63 mm
12 mm
blue Tubing Adapter.