CMA Dishmachines 11 User Manual

CMA 11 Microdialysis Probe User's Manual
intlet
steel shaft
membrane

Instructions for CMA 11 Microdialysis Probe

1. Fill a microsyringe with perfusion fluid and mount it in the CMA Microinjection Pump. The Perfusion Fluid must be
clean, at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula.
3. Mount the microdialysis probe in the CMA 11 & 12 Probe/Guide Clip on the CMA/130 in vitro Stand. Connect the de-
sired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet. Tubing Adapters
and FEP-Tubing should be used for all connections. Put the microdialysis probe in a vial filled with perfusion fluid. To
facilitate the handling of Tubing Adapters, they should be presoaked in ethanol for a minimum of 10 minutes.
4. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the cannula.
5. Flush the probe, lowered in a vial filled with 70% ethanol. Pump the perfusate for 4-5 min. to wash out the glycerol.
6. Put the probe back into the vial filled with perfusion fluid. Flush for another 4-5 min. to wash out the ethanol and air. Use
the following flow rates:
Check for air bubbles inside the membrane with a stereomicroscope. The membrane may appear to be "sweating" which
is due to the ultrafiltration of fluid through the membrane.
7. Set the pump to the required perfusion flow (usually 1-5 µL/min.) and check for leaks. The microdialysis probe is now
ready for use.
8. When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL
INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short
sampling times.
9. After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to
prevent salt crystal formation.
outlet
shaft 0.40
0.24
Flow
50 μL/min
40 μL/min
15 μL/min
NEVER EXCEED STATES FLOW RATES!

TECHNICAL INFORMATION

Membrane
Material
Molecular Cut-Off
Outer Diameter
Length
Probe Shaft
Material
Diameter
Length
Internal Volume
Inlet Volume
Outlet Volume
Probe outlet tubing
100 mm long
200 mm long
300 mm long
Cuprophane
6,000 Daltons
0.24 mm
1, 2, 3 and 4 mm
Stainless-steel or Metal Free
0.40 mm
14 mm
Negligible
1 µL