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BAS SEC2000 Spectra System Operating Manual

Uv/visible spectrophotometer
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  • Page 2 - Windows, Windows 2000, Windows XP and Windows 7 are either registered trademarks or trademarks of Microsoft Corporation. December 2010 SEC2000 Spectra System www.als-japan.com...
  • Page 3 We always make our effort to be the world’s best company in the analysis system market with continuous commitment to make the world’s best product. Customer Service BAS Inc. TEL : +81-3-3624-0331 FAX : +81-3-3624-3387 E-Mail : sales@bas.co.jp Homepage : http://www.bas.co.jp/ SEC2000 Spectra System www.als-japan.com...
  • Page 4 ▪ Do not spray the water to clean the instrument. ▪ Make sure the cables are not twisted or bent. ▪ Do not pull the cables with fierce force. ▪ Do not dismantle and modify the system. SEC2000 Spectra System www.als-japan.com...

  • Page 5: Table Of Contents

    5. Measurement 5-1. Calibration 5-2. Dark and Reference Measurements 5-3. Sample Measurement 5-4. Absorbance/ Transmittance Measurement 5-5. Reflectance Measurement 5-6. Irradiance Measurement 5-7. Time Series Acquisition 6. Analysis 6-1. Overlay 6-2. Quantitative Analysis 7. Multi-Channel 8. Trigger SEC2000 Spectra System www.als-japan.com...

  • Page 6: Introduction

    Item Description Item Code SEC2000-UV/VIS Detector SEC2000 Spectrometer SEC2000-VIS/NIR Software Visual Spectra 2.1 Visual Spectra 2.1 Tungsten-Halogen Lamp (for Visible) SEC2000-TH Light Source Deuterium & Tungsten Halogen Lamp (for UV-Visible) SEC2000-DH Cuvette holder 3-Way Holder SEC2000-CUV SEC2000 Spectra System www.als-japan.com...

  • Page 7: Operating Principle

    1-3-3. Specifications A miniature spectrometer, SEC2000 couples a reasonable cost and high-performance with 2,048-element linear CCD-array detector. Thanks to a modular approach, the components are easily mixed and matched, which offers remarkably flexible applications. SEC2000 Spectra System www.als-japan.com...

  • Page 8: Application

    ~1.2 nm (25 m slit, option) Sensor Silicon Charge Coupled Device (Si-CCD)-2,048 pixels Grating 600 lines/mm Physical dimension 35 x 98 x 250 mm Integration time 12~13 scans/second 1-4. Application 1-4-1. System Modes Absorbance/Transmittance Mode Reflectance/Fluorescence Mode Irradiance Mode <Fig. 1-4-1> SEC2000 Spectra System www.als-japan.com...
  • Page 9  Environmental fields (Waste water analysis, Ground water trace Fluorescence studies, Hydrocarbon detection, Dissolved oxygen)  Plant efficiency (Plant physiology, Plant breeding, Horticulture, Agronomy, Agrochemicals, Pollution studies, Forestry, Ecology)  Tissue diagnosis Reflection  Concentration in solution Index SEC2000 Spectra System www.als-japan.com...

  • Page 10: Sec2000

    SEC2000 Operating Manual 2. SEC2000 2-1. Composition  Product: SEC2000 spectra system  Power source: 12 V adaptor/ input free volt  Interface cable: USB cable  Software: Visual Spectra 2.1 2-2. Specifications <Table 2-2-1> Platform  Portable size (35 x 98 x 250 mm) ...

  • Page 11: Installation

    3-2. Software & Diver Installation Open the VSpec2.1Sr software folder and click setup.exe as shown in Fig.3-2-1. Follow the instructions of the setup program. <Fig. 3-2-1> First click Next in the window shown in Fig. 3-2-2. <Fig. 3-2-2> SEC2000 Spectra System www.als-japan.com...
  • Page 12 Click Next to move on. <Fig. 3-2-4> Please select the Complete setup type, and click Next. If you are satisfied with your installation, select Install. The icon of Visual Spectra 2.1 is created on your computer screen. SEC2000 Spectra System www.als-japan.com...
  • Page 13 SEC2000 Operating Manual <Fig. 3-2-5> Next, you should install the diver of the hardware. Open the Divers folder from the VSpec2.1Sr software source folder. <Fig. 3-2-6> SEC2000 Spectra System www.als-japan.com...
  • Page 14 SEC2000 Operating Manual Select the suitable diver and click the program, follow the InstallShield Wizard to install the diver. <Fig. 3-2-7> <Fig. 3-2-8> Clicking Finish to complete installation of the diver as shown in Fig 3-2-9. <Fig. 3-2-9> SEC2000 Spectra System www.als-japan.com...

  • Page 15: Connect Sec2000 To The Pc

    Using the enclosed USB cable, connect SEC2000 to the PC as shown in Fig.3-3-1. <Fig. 3-3-1> 3-3-1. Windows XP The computer automatically recognizes the USB interface. In the Found New Hardware Wizard screen, check Install software automatically (Recommended) and click the Next. <Fig. 3-3-2> SEC2000 Spectra System www.als-japan.com...
  • Page 16 SEC2000 Operating Manual When the Hardware installation is completed, click the Finish. <Fig. 3-3-3> 3-3-2. Windows 7 The PC will recognize SEC2000 and you can start to operate the spectrometer. <Fig. 3-3-4> SEC2000 Spectra System www.als-japan.com...

  • Page 17: Operation

    <Fig. 4-1-1> Connect the Spectra System detector to a USB interface of the computer using the enclosed USB cable and plug in the enclosed electrical cord to Light Source. Then turn on the light. <Fig. 4-1-2> SEC2000 Spectra System www.als-japan.com...

  • Page 18: Program Start

    If the Spectra System driver is not installed properly or the USB connection fails between Spectra System and your computer, Failed in loading SEC2000 device message will appears. In this case, check the USB connection or reinstall the USB driver. <Fig. 4-2-2> SEC2000 Spectra System www.als-japan.com...

  • Page 19: Software Operation

    Save the Master Trace’s data to file (processed spectrum, saved as *.absorb/ *.trans/ *.irrad.). Processed It is activated when the dark and reference spectra are saved. Establish Establish environment for a measuring spectrum and save the environment graphical view setting file (*.ini). SEC2000 Spectra System www.als-japan.com...
  • Page 20 Ex. of file name: ALS1204,date→20110112, etc. Set the starting index for auto incremented files. Starting Index Ex. of file name: ALS1204-1, ALS1204-2, etc. - Print Setup Select and configure a printer for graphical spectrum printing <Fig. 4-3-1> SEC2000 Spectra System www.als-japan.com...
  • Page 21 SEC2000 Operating Manual - Page Setup Write Title, Comment and Footer for printing graphical spectrum. <Fig. 4-3-2> - Print Preview Preview the graphical spectra printing. <Fig. 4-3-3> - Print (Ctrl+P) Print current graphical spectra. - Exit End program. SEC2000 Spectra System www.als-japan.com...
  • Page 22 Make a marker for current graphical spectrum. <Fig. 4-3-4> - Erase Marker (Ctrl+E) Erase a maker that is located near a current cursor. - Erase All Markers Erase all markers. ③ View - Rename Window Rename a graphical spectrum window. <Fig. 4-3-5> SEC2000 Spectra System www.als-japan.com...
  • Page 23 Background Choose a background color for the spectral window. Frame Choose a frame color for the spectral window. Cursor Choose a cursor color for the spectral window. Grid Choose a grid color for the spectral window. SEC2000 Spectra System www.als-japan.com...
  • Page 24 Toggle the tool bar display in the main visual spectral window. <Fig. 4-3-6> If the“Quantitative Analysis” is checked, the window will be displayed on the right side of the main window as below figure. <Fig. 4-3-7> SEC2000 Spectra System www.als-japan.com...
  • Page 25 Switch the current spectral window into scope mode and subtract the stored dark spectrum. Absorbance Mode Display data in absorbance mode. Please save the Dark and Reference spectra before using this function. Transmittance Mode Display data in transmittance mode. Please save the SEC2000 Spectra System www.als-japan.com...
  • Page 26 <Fig. 4-3-9> Quantitative Analysis It is effective only on absorbance mode. Peak Search Search peaks in the specified range. Click Save, the peak data will be saved as *.csv file. <Fig. 4-3-10> SEC2000 Spectra System www.als-japan.com...
  • Page 27 Configure Time Acquisition Configure and establish the parameters for time acquisition process. <Fig. 4-3-11> - Configure Time Acq. Channel Set up the channels for time acquisition process. Six specific wavelengths can be set up from channel 1 to channel 6. <Fig. 4-3-12> SEC2000 Spectra System www.als-japan.com...
  • Page 28 - Cascade Arrange all spectral windows in a horizontally overlapping design. - Tile Arrange all spectral windows side by side. ⑧ Help - Help Topics Present the user manual for the system. - About Visual Spectra 2.1 Present the version information of Visual Spectra 2.1. SEC2000 Spectra System www.als-japan.com...
  • Page 29 With this function selected, clicking on a point will provide the coordinate information (Wavelength, Intensity) shown on the status bar at the bottom of the screen. In accordance with the movement of the mouse, new coordinate information will be shown. SEC2000 Spectra System www.als-japan.com...
  • Page 30 If the figure is too high, the limit of resolution lowers. Decide the lowest figure so the spectral signal does not vary much. When you want to separate intensities of more than two nearby wavelengths, lower this function to meet your experiment. SEC2000 Spectra System www.als-japan.com...

  • Page 31: Measurement

    ① The power supply is switched on and turned ON the UV lamp and halogen lamp. Wait for 20 to 30 minutes until UV lamp is stabilized. ② Put the reference sample into the cuvette holder, and open the light source shutter SEC2000 Spectra System www.als-japan.com...

  • Page 32: Sample Measurement

    Where A is the absorbance of the solution,  is the molar absorptivity（mol is the pathlength of radiation through the absorbing medium (cm) and c is the concentration (mol/L) of the solution. The absorbance is measured with the following procedure. SEC2000 Spectra System www.als-japan.com...
  • Page 33 “File”/”Save”/”Processed” or “Sample” to save an absorbance or transmittance spectrum. Notes When absorbance/transmittance is saved by “File”/”Save”/”Processed”, the data of absorbance/transmittance is displayed only absorbance/transmittance mode. However, if absorbance/transmittance is saved “File”/”Save”/“Sample”, the saved data is convertible for other mode. SEC2000 Spectra System www.als-japan.com...

  • Page 34: Reflectance Measurement

    <Fig. 5-5-1> Configuration example of reflectance measurement. ① First, the light source and detector of SEC2000 are set as shown in Fig. 5-5-1. ② The power supply is switched on and turned ON the UV lamp and halogen lamp. SEC2000 Spectra System www.als-japan.com...
  • Page 35 In this measurement, the Reflection probe is used. The reflectance is measured with the following procedure. SEC2000-UV/VIS SEC2000-D Computer Reflection Probe Probe holder Reflectance Standard Sample <Fig. 5-5-2> Configuration example of reflectance measurement using reflection probe. SEC2000 Spectra System www.als-japan.com...

  • Page 36: Irradiance Measurement

    ① First, connect the detector of SEC2000 and integrating sphere by optical fiber. Set the standard light source to integrating sphere as shown in Fig. 5-6-1. ② Turn on the power of the standard light source. Wait for 20 to 30 minutes until the SEC2000 Spectra System www.als-japan.com...
  • Page 37 Save the dark spectrum by “File”/”Save”/”Dark”. ⑧ Connect a luminescence sample to integrating sphere (set a LED bulb to integrating sphere), Click the “Irradiance Mode” icon to change the Mode. <Fig. 5-6-2> Relative irradiance measurement of LED bulb. SEC2000 Spectra System www.als-japan.com...

  • Page 38: Time Series Acquisition

    5-7. Time Series Acquisition (TSA) Time Series Acquisition is monitoring of the intensity change of absorbance, transmittance and radiation illumination. Time course of chemical and physical changes of the sample is measured. This function is applicable to a chemical substance, SEC2000 Spectra System www.als-japan.com...
  • Page 39 Reflectance modes. Doing time series acquisition measurement, please follow the steps: ① First, open “Menu”/”Time”/”Configure Time Acquisition”, or click the icon. ② The following window will appear. Configure and establish the parameters for time acquisition process. <Fig. 5-7-1> SEC2000 Spectra System www.als-japan.com...
  • Page 40 ⑥ Enter the “Duration” to set the data collection period. ⑦ Open “Menu”/”Time”/”Configure Time Acquisition Channel”, or click the icon. In this window you can configure the wavelength channels. Six specific wavelengths can be set up from channel 1 to channel 6. SEC2000 Spectra System www.als-japan.com...
  • Page 41 Channels at the same time, fill one figure in “Offset” and increase the plot value. Fill it with real value only when the plots are overlapped. ⑧ Combinations 1 and 2 can perform operation among channel 1 ~ channel 6. SEC2000 Spectra System www.als-japan.com...
  • Page 42 ”Monitor 1” is for channel monitoring. The lower window ”Spectrum 1” is for original spectrum. Click the start “►” icon of “Monitor 1”, then the data collection will be carried out on the set-up conditions. SEC2000 Spectra System www.als-japan.com...
  • Page 43 SEC2000 Operating Manual <Fig. 5-7-4> ⑪ The saved full spectrum files can be displayed using “Overlay”/”Time series”/”Add overlay (Multiple Selection)” SEC2000 Spectra System www.als-japan.com...
  • Page 44 SEC2000 Operating Manual <Fig. 5-7-5> ⑫ The data can be displayed by 3D figure after clicking the icon. <Fig. 5-7-6> SEC2000 Spectra System www.als-japan.com...

  • Page 45: Analysis

    Please proceed to the following steps to concrete your data. 6-1-1. Overlay of Time Series data ① Open the software. <Fig. 6-1-1> ② Open a “*.timeall“ data by “Overlay”/”Time series”/”Add Overlay”. <Fig. 6-1-2> SEC2000 Spectra System www.als-japan.com...
  • Page 46 ④ Adjust the scale, specify the optimal range, and then the data are displayed. The legend is shown in the right side of the graph. The data name is shown at the time of the measurement of the time course. <Fig. 6-1-4> SEC2000 Spectra System www.als-japan.com...
  • Page 47 10 files at the same time. <Fig. 6-1-6> ⑧ Adjust the scale, specify the optimal range, and then the data are displayed. There is no legend. Place your mouse on the lower left color icon (11 pieces), and then SEC2000 Spectra System www.als-japan.com...
  • Page 48 <Fig. 6-1-7> 6-1-3. Overlay of Processed data ① To overlay a processed data (*.absorb/*.trans/*.refl/*.irrad), you must install the Dark and Reference data first. Open any sample data by “File”/”Open/ ”Sample”. <Fig. 6-1-8> SEC2000 Spectra System www.als-japan.com...
  • Page 49 ② "Dark" and "Reference" which were shown by gray become effective. <Fig. 6-1-10 ③ Open the dark data saved in the same condition as saving processed data, by “File”/”Open”/”Dark”. Then open the reference data. <Fig. 6-1-11> SEC2000 Spectra System www.als-japan.com...
  • Page 50 ④ Dark and reference spectra are automatically overlaid to Overlay1 and 2. The icons of Absorbance/ Transmittance/ Reflectance/ Irradiance become effective. <Fig. 6-1-12> ⑤ Change the mode to Absorbance Mode. Then overlay a “*.absorb” data by “Overlay”/”Spectrum”/”Add Overlay”. <Fig. 6-1-13> SEC2000 Spectra System www.als-japan.com...
  • Page 51 <Fig. 6-1-14> ⑦ To overlay a *.trans/*.refl/*.irrad data, same to the step ⑤, change the mode to Transmittance/Reflectance/Irridiance Mode, and then overlay the data by “Overlay”/ ”Spectrum”/ ”Add Overlay”. <Fig. 6-1-15> SEC2000 Spectra System www.als-japan.com...

  • Page 52: Quantitative Analysis

    Put a standard sample into the cuvette holder in absorbance mode, then the screen will become as Fig. 6-2-2. The following procedures make 1 mM potassium ferricyanide as an example. <Fig. 6-2-2> Absorbance of 1 mM K Fe(CN) solution. SEC2000 Spectra System www.als-japan.com...
  • Page 53 ④ Set the ”Baseline Correction” range according to Fig. 6-2-4. Baseline Correction range Absorbance Area <Fig. 6-2-4> ⑤ Set the ”Absorbance Area” according to Fig. 6-2-4. Click “Next”. ⑤ ④ <Fig. 6-2-5> Selection of wavelength range for the absorbance area and Baseline Correction. SEC2000 Spectra System www.als-japan.com...
  • Page 54 (Concentration: 1, 0.5 and 0.25 mM). ⑦ Click “Use”, then calibration line is obtained as shown in Fig. 6-2-7. <Fig. 6-2-7> Calibration line with three different concentrations. The standard samples are shown in closed circle. SEC2000 Spectra System www.als-japan.com...
  • Page 55 Analyze the concentration of a sample by making calibration line with the peak absorbance of standard solutions. It is possible only in absorbance mode. ① Set the spectrometer system as shown in Fig. 6-2-8. <Fig. 6-2-8> Configuration for an absorbance experiment SEC2000 Spectra System www.als-japan.com...
  • Page 56 Set the peak wavelength of the sample by “Wavelength_ nm”, we set 413 nm  for the ferricyanide. Set the unit of solution concentration by “Con.”, we choose “mM”.  Set the unit of absorbance by “Intensity”, we choose “O.D. ”.  SEC2000 Spectra System www.als-japan.com...
  • Page 57 ⑤ Input the standard concentration of “Sample 1” to “Sample 5”, and click “>>” The vertical axis of the peak is read. <Fig. 6-2-11> ⑥ Change the standard, and get the peak absorbance of each concentration. SEC2000 Spectra System www.als-japan.com...
  • Page 58 ⑧ Put an unknown sample into the cuvette holder, and click “Meas.” icon. The peak absorbance of the sample is measured and displayed in “O.D.”, and the concentration is displayed in “ mM” as following Fig. 6-2-13. <Fig. 6-2-13> SEC2000 Spectra System www.als-japan.com...

  • Page 59: Multi-Channel

    USBHUB with the power supply to connect the spectrometers. And you can confirm the connection from “Device Manager” as shown in Fig.7-2-1. <Fig.7-2-1> ② Open the software, it shows as [Spectrum1]. Click the “GO” icon, the spectrometer set as [CH1] becomes effective (Fig.7-2-2). <Fig.7-2-2> SEC2000 Spectra System www.als-japan.com...
  • Page 60 ⑤ The time course measurement for each channel is displayed as [Monitor1] and [Monitor2]. It indicates opening number in turn, but not corresponds to Channel number. As shown, for example, in Fig. 7-2-5, Monitor1 corresponds to CH1, and Monitor2 corresponds to CH3. SEC2000 Spectra System www.als-japan.com...
  • Page 61 SEC2000 Operating Manual <Fig.7-2-5> SEC2000 Spectra System www.als-japan.com...

  • Page 62: Trigger

    <Fig. 8-1-1 External Trigger Signal: Low level> 8-2. External trigger Fig.8-2-1 shows the Trigger In connector. The black wire is for Ground (GND) and the yellow wire is for Trigger In. Trigger In: 5 V <Fig. 8-2-1 External Trigger connector> SEC2000 Spectra System www.als-japan.com...
  • Page 63 <Fig. 8-3-2 > ③ Set the “Configure Time Acquisition” (Fig. 8-3-3) at “Ext. Trigger” “Normal” condition(Fig.8-3-2). The detail of Time acquisition setting is described in 5-7. of the Chapter 5. <Fig. 8-3-3 > SEC2000 Spectra System www.als-japan.com...
  • Page 64 ⑤ Click the “START” icon in the Monitor1 screen. During this time the external TTL is 5 V. Waiting for the change of the external TTL, all screens are on standby, and the spectrum is in condition of the stop state (Fig. 8-3-5). <Fig. 8-3-5 > SEC2000 Spectra System www.als-japan.com...
  • Page 65 ⑥ When the external TTL changes from 5 V to 0 V, the spectrum measurement is begun, and the spectrum and the monitor screens start to move (Fig. 8-3-6). <Fig. 8-3-6 > ⑦ To stop the monitor, you can configure the “Duration” or click the “STOP” icon in the Mornotor1 screen. SEC2000 Spectra System www.als-japan.com...
  • Page 66 SEC2000 Operating Manual SEC2000 Spectra System www.als-japan.com...

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