Summary of Contents for Aptum 2100 Retriever
- Page 1 Unique technology to retrieve the antigens on fixed, paraffin-embedded tissue sections US Patent Pending 2007/0227298A1 Retriever 2100 OPERATION MANUAL Since 2007...
- Page 2 INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTION WHAT IS THE RETRIEVER 2100 2100- - - - Retriever Retriever, or, from now on simply Retriever, is a bench-top model for 2100 2100 Retriever Retriever thermally processing slides of the formalin-fixed, paraffin embedded tis- sues prior to immunostaining. The model is designed to ensure identical processing of all the samples during a processing cycle, as well as the identical processing of the samples between individual sessions.
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Page 3: Table Of Contents
CONTENT CONTENT CONTENT CONTENT Retriever and Acessories (Scheme) Getting Started Fixing Tissue Samples Cuttung Tissue Sections Dewaxing Tissue Sections Choosing the Buffer Processing Sections in Retriever Immunostaining Examples of Staining Do’s and Don’t’s Care and Maintanance Troubleshooting Safety Features of Retriever Certificates of Retriever Technical Specifications... -
Page 4: Retriever And Acessories (Scheme)
YOUR RETRIEVER YOUR RETRIEVER YOUR RETRIEVER YOUR RETRIEVER The supplier Retriever set contains the processing unit, slide chamber (allows to fit 18 Slides) and Chamber Rack for 6 Slide Chambers (108 slides in total). On the control panel the Start Button and indicator lights are located that show different stages of the processing program. -
Page 5: Getting Started
GETTING STARTED GETTING STARTED GETTING STARTED GETTING STARTED Retriever is an autoclave Retriever is an autoclave and thus has to be handled appropriately and Retriever is an autoclave Retriever is an autoclave with care. Before using the Retriever for the first time please take time to read Before using the Retriever for the first time please take time to read Before using the Retriever for the first time please take time to read Before using the Retriever for the first time please take time to read... -
Page 6: Fixing Tissue Samples
FIXING TISSUE SAMPLES FIXING TISSUE SAMPLES FIXING TISSUE SAMPLES FIXING TISSUE SAMPLES We have developed the Retriever for formalin fixed, paraffin embedded tissues, the most routine material available from tissue collections and easy to prepare from fresh tissue samples. A routine protocol used in the majority of pathology laboratories is sufficient. -
Page 7: Cuttung Tissue Sections
CUTTING TISSUE SECTIONS CUTTING TISSUE SECTIONS CUTTING TISSUE SECTIONS CUTTING TISSUE SECTIONS PROTOCOL All paraffin embedded tissue is cut at a thickness of 3 µm. Thicker All paraffin embedded tissue is cut at a thickness of 3 µm. Thicker All paraffin embedded tissue is cut at a thickness of 3 µm. Thicker All paraffin embedded tissue is cut at a thickness of 3 µm. -
Page 8: Dewaxing Tissue Sections
DE- - - - WAXING SECTIONS WAXING SECTIONS WAXING SECTIONS WAXING SECTIONS PROTOCOL De-wax paraffin sections (this procedure is identical irrespective of the fixative used for the tissue). Place section(s) into staining vessels, for ex- ample, Coplin jars, and treat as follows: Xylene, three changes, 5 min in each Xylene, three changes, 5 min in each Xylene, three changes, 5 min in each... -
Page 9: Choosing The Buffer
CHOOSING THE BUFFER CHOOSING THE BUFFER CHOOSING THE BUFFER CHOOSING THE BUFFER Two important issues determine the choice of the buffer: The nature of the antigen and of the antibody used for its detection The fixative used and the degree of fixation. If your laboratory has good experience with a routine Citrate (pH6) and EDTA (pH 8 or 9) buffers you may continue using those. -
Page 10: Processing Sections In Retriever
PROCESSING SECTIONS PROCESSING SECTIONS PROCESSING SECTIONS PROCESSING SECTIONS PROTOCOL Place the slides into Retriever Slide Chamber and immediately fill Place the slides into Retriever Slide Chamber and immediately fill Place the slides into Retriever Slide Chamber and immediately fill Place the slides into Retriever Slide Chamber and immediately fill them with the processing buffer of your choice ( see the page 8 for them with the processing buffer of your choice ( see the page 8 for them with the processing buffer of your choice ( see the page 8 for... - Page 11 PROCESSING SECTIONS PROCESSING SECTIONS PROCESSING SECTIONS PROCESSING SECTIONS Slide Chamber. Up to 18 slides fits one chamber. Place the slides in the same orienta- tion of the section side Place Slide Chambers into the Rack and pour the processing buffer into the Chambers to fill all free space.
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Page 12: Immunostaining
IMMUNOSTAINING IMMUNOSTAINING IMMUNOSTAINING IMMUNOSTAINING PROTOCOL After processing in Retriever use the sections within the same day After processing in Retriever use the sections within the same day After processing in Retriever use the sections within the same day After processing in Retriever use the sections within the same day Rinse the sections in PBS for 5 min. -
Page 13: Examples Of Staining
WHAT YOU MUST GET WHAT YOU MUST GET WHAT YOU MUST GET WHAT YOU MUST GET EXAMPLES OF PROCESSED TISSUE Compare these three sections of squamous epithelium: Section A A A A was untreated, section B B B B was processed in a microwave, and section C C C C - in Retriever. -
Page 14: Do's And Don't's
CONTINUED OPERATION CONTINUED OPERATION CONTINUED OPERATION CONTINUED OPERATION To ensure your Retriever gives you the years of service for which it was designed, it is important to remember a few "do's" and "don'ts" with re- gard to the operation of the unit and to carry out the simple care and maintenance procedures on a weekly basis. - Page 15 CONTINUED OPERATION CONTINUED OPERATION CONTINUED OPERATION CONTINUED OPERATION 7..open the Depressurization Valve (A) during the cycle. 8... leave the Depressurization Valve (A) in the "open" position when plac- ing the lid upside down on a work surface. 9...immerse the unit or electrical cord in water when cleaning. 10...use abrasive materials or lubricants when cleaning.
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Page 16: Care And Maintanance
CARE & MAINTANANCE CARE & MAINTANANCE CARE & MAINTANANCE CARE & MAINTANANCE WARNING! Disconnect the Retriever from the main’s power supply be- WARNING! Disconnect the Retriever from the main’s power supply be- WARNING! Disconnect the Retriever from the main’s power supply be- WARNING! Disconnect the Retriever from the main’s power supply be- fore cleaning fore cleaning. -
Page 17: Troubleshooting
TROUBLESHOOTING TROUBLESHOOTING TROUBLESHOOTING TROUBLESHOOTING Fault 1: Fault 1: Fault 1: Fault 1: Blown fuse / Defec- Ensure mains lead is connected. Check / Power to Unit tive Socket / Mains replace fuse: Check power to socket: not connected Light: L1 fails to illuminate Fault 2: Fault 2:... -
Page 18: Safety Features Of Retriever
SAFETY FEATURES SAFETY FEATURES SAFETY FEATURES SAFETY FEATURES In the event of a fault occurring during any stage of the units operation, identify the fault by referring to the descriptions below. The fault con be rectified by following the Fault Remedy applicable to the problem in- curred.In the unlikely event that something should go wrong, we have in- we have in-... -
Page 19: Certificates Of Retriever
STANDARDS STANDARDS STANDARDS STANDARDS Prestige Medical, a manufacturer of the 2100-Retriever ( belongs to the 2100 Series) has always been proud of its reputation for quality and inno- vation in the design and production of our sterilization systems. Prestige Medical has continually strived to be at the forefront of the industry with regards to meeting and even exceeding those standards which apply to it as a manufacturer and the products it makes: ISO 9001 (Certificate No. -
Page 20: Technical Specifications
TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS TECH ICAL SPECIFICATIO S OF RETRIEVER 2001 Height ....335 mm Capacity ....9 litres Max. Instrument length..228 mm Width ....340 mm Internal chamber Net Weight ..4.5 kilos Dimensions (d/h)..210/230 mm Max.
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