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Summary of Contents for World Precision Instruments PUL-1000
- Page 1 WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas INSTRUCTION MANUAL PUL-1000 Microprocessor-controlled 4-Step Micropipette Puller Serial No._____________________ www.wpiinc.com 082318...
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Page 3: Table Of Contents
Claims and Returns ........................15 Repairs ............................15 Copyright © 2018 by World Precision Instruments. All rights reserved. No part of this publication may be reproduced or translated into any language, in any form, without prior written permission of World Precision Instruments, Inc. -
Page 4: Quick Start
NOTE: Program sequence 00 is designed for OD 1.0mm borosilicate capillary glass (WPI# 1B100-4), and sequence 01 is designed for OD 1.14mm borosilicate capillary glass (WPI# 4878). Close the cover. Press START, and the puller will do the rest. Each pull results in two identical micropipettes. World Precision Instruments... -
Page 5: About This Manual
NOTES and TIPS contain helpful information. Fig. 1 The PUL-1000 is programmable. INTRODUCTION PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making glass micropipettes or microelectrodes used in intracellular recording, patch clamp, microperfusion and microinjection. The puller was designed with tight mechanical specifications and precision electronics for complete control of the pulling process and accurate reproducibility. -
Page 6: Features
A glass capillary is heated by a platinum/iridium filament and pulled by a controlled force. The PUL-1000 is capable of producing a vast array of pipette shapes. Pipettes–PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to 10+ µm. -
Page 7: Unpacking
INSTRUMENT DESCRIPTION Tempered Glass Lid Capillary Glass Clamps Heating Filament V-Shaped Glass Groove Carriage Fig. 2 The parts of the puller are identified. Tempered Glass Lid–For safety, the lid should be closed whenever you are pulling glass. World Precision Instruments... - Page 8 Capillary Glass Clamps–These two clamps securely hold the glass when you are pulling pipettes. Heating Filament–The PUL-1000 comes with a platinum/iridium box filament, which may be easily replaced, as needed. (See “Accessories” on page 10.) V-Shaped Glass Groove–Slide a single piece of capillary glass into the groove from one side or the other.
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Page 9: Program Sequence
PUL-1000 Program Sequence A saved program can be loaded into the PUL-1000 microprocessor. When the LCD cursor is flashing next to the entry “LOAD”on the display, use the arrows on the keypad to select a program. NOTE: The puller is shipped with programs saved in memory locations 00 – 01. -
Page 10: Program Run Sequence
When the unit is powered on, the LCD screen displays the last used sequence in the window. PUL-1000 is designed to form the pipette in four steps. A basic program step (called a line) consists of four entries:... -
Page 11: Program Parameters
NOTE: The force applied may be set as a constant force or a gradient force. The constant force applies the same amount of force throughout the entire program. A gradient force allows the force to slowly taper off as the program runs. When the World Precision Instruments... -
Page 12: Glass Capillary Softening Test
The heating stops when the glass begins to move. Record the heating power. This is the baseline heating value for the glass type tested. It is a good starting point for the first stage of your program. World Precision Instruments... -
Page 13: Maintenance
Side View Top View Support Support Correct filament placement Block Block Glass centered through filament Wrong filament placement Fig. 8 Position the filament so the capillary glass runs through the center of the filament. World Precision Instruments... -
Page 14: Choosing A Filament
Capillary OD Range ..................1.0–2.0mm Maximum Capillary Length ................... 170mm Minimum Capillary Length ..................55mm Permanent Memory Set ....................95 Auto Shut-off Time ....................... 90 s Power Input ................100-240VAC 1.5A 50-60Hz Power Output ....19V DC at 4.74A (5.5x2.5 barrel connector with positive tip) Dimensions ....................37 x 24 x 15cm Shipping Weight ......................16 lb. NOTE: This puller is designed for single barrel borosilicate glasses or patch glass, not quartz or aluminosilicate. World Precision Instruments... -
Page 15: Troubleshooting
Remove the glass, slide the carriages may be too far apart. together and reposition the glass. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. World Precision Instruments... -
Page 16: Appendix A: Example Sequences
HEAT FORCE DISTANCE DELAY 2.00 0.70 0.30 0.30 OD: 1.14mm (WPI# 4878). STEP HEAT FORCE DISTANCE DELAY 3.00 1.00 0.30 0.30 My Sequences Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY World Precision Instruments... - Page 17 PUL-1000 Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY Name: _____________________________________________ STEP HEAT FORCE DISTANCE DELAY World Precision Instruments...
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Page 19: Warranty
PUL-1000 WARRANTY WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, including its components and parts, shall be free from defects in material and workmanship for a period of 30 days* from the date of receipt. WPI’s obligation under this warranty shall be limited to repair or replacement, at WPI’s option, of the equipment or defective components or parts upon receipt thereof... - Page 20 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Saarstraße 23, D-61169 Friedberg (Hesson), Germany Tel: +49 (0)6031 1602171 •...
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