Grace Bio-Labs CultureWell Instructions For Use

Chambered coverslips

Advertisement

 
1. Place the MultiWell coverslip package onto a work surface as shown in Figure 1.
Note: Package contents are sterile, handle carefully to prevent contamination.
2. Aseptically peel back the sterile pouch and top plastic liner to expose coverslips as shown in
Figures 1 and 2.
3. Using a sterile forceps, lift the coverslip by grasping the silicone as shown in Figure 3.
 
|
CultureWell™ MultiWell Chambered Coverslips
Figure 1
Figure 2
Figure 3
Instructions for Use
|

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the CultureWell and is the answer not in the manual?

Questions and answers

Summary of Contents for Grace Bio-Labs CultureWell

  • Page 1   Instructions for Use CultureWell™ MultiWell Chambered Coverslips 1. Place the MultiWell coverslip package onto a work surface as shown in Figure 1. Note: Package contents are sterile, handle carefully to prevent contamination. Figure 1 2. Aseptically peel back the sterile pouch and top plastic liner to expose coverslips as shown in Figures 1 and 2.
  • Page 2 Instructions for Use CultureWell™ MultiWell Chambered Coverslips 4. Remove the lid from a CultureWell plate or sterile 100mm diameter culture dish. Place the coverslip into the plate or dish as shown in Figure 4 with the silicone gasketed wells facing up.
  • Page 3   Instructions for Use CultureWell™ MultiWell Chambered Coverslips 6. After cells attach, flood the tray with 20ml of culture medium as shown in Figure 6. Alternately, plates and coverslips may be flooded with a suspension of cells in medium (20ml-Plates) and incubated to allow cells to attach to coverslips.
  • Page 4   Instructions for Use CultureWell™ MultiWell Chambered Coverslips 9. Coverslips are removed with the silicone gasket still attached to provide stability and protection from breakage. Figure 8 shows how individual gasketed coverslips may be placed in coplin jars or any other holder designed for standard microscope slides.
  • Page 5 Place a clean, dry 24mm x 50mm glass coverslip onto the silicone gasket and press gently to seal. Place gasketed coverslips individually into glass petri dishes (silicone side up), wrap with aluminum foil and sterilize by autoclaving. Allow coverslips to cool and dry completely before use. CultureWell™ MultiWell chambered coverslips are for laboratory use only. 5   ...

Table of Contents