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3DHISTECH Ltd. Pannoramic DESK II User Manual

Software version: pannoramic scanner 1.23 or higher
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Pannoramic DESK II
1.23
User's Guide
June 4, 2018
Rev. 2
PDII123-UG-R
For research and education uses only, not for use in diagnostic procedures.

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Summary of Contents for 3DHISTECH Ltd. Pannoramic DESK II

  • Page 1 Pannoramic DESK II 1.23 User’s Guide June 4, 2018 Rev. 2 PDII123-UG-R For research and education uses only, not for use in diagnostic procedures.

  • Page 2: Table Of Contents

    3 Preparing Slides......................20 3.1 Affixing Barcode Stickers to Slides..................21 3.2 Inserting and Removing Slides....................22 4 Software Description....................29 4.1 Starting / Exiting Pannoramic DESK II Software Application...........29 4.1.1 Starting the software.............................29 4.1.2 Exiting the software............................29 4.2 Options............................29 4.2.1 Barcode Settings............................29 4.2.2 Image Compensation.............................34...
  • Page 3 Pannoramic DESK II 1.23 User’s Guide 4.3.4 Preview area scanned by preview camera....................52 4.3.5 Focus tab................................57 4.3.6 Service tab..............................58 4.3.7 Display field and toolbar for live images.......................58 4.3.8 Start Scan...............................60 4.4 Scan To Server.........................61 4.5 Using Profiles..........................66 5 Troubleshooting and Maintenance................67 5.1 Safety Information........................67...

  • Page 4: Disclaimer

    Pannoramic DESK II 1.23 User’s Guide Disclaimer Copyright © 2001-2017 3DHISTECH Ltd. All rights reserved. Intellectual property related to the 3DHISTECH products are as follows: EP 1,789,831; US 7,663,078; EP 2,024,772; JP 2009-512685; US 12/301,781; EP 1,644,767; JP 2007- 516451; US7,327,515; EP 1,994,398; JP 2008-557835; US 12/281,566; U0700210; US 6,246,785;...

  • Page 5: June

    Pannoramic DESK II 1.23 User’s Guide Further Information For the latest information on 3DHISTECH products and services, please visit our website at the following URL: http ://www.3dhistech.com Or contact the distributor: Richard Allan Scientific Co. Subsidiary of Thermo Fisher Scientific...

  • Page 6: Declaration Of Conformity

    Pannoramic DESK II 1.23 User’s Guide Declaration Of Conformity 3DHISTECH Ltd. declares that the product Pannoramic DESK II slide scanner is designed and produced with consideration of specified requirements according to the IEC 61326-2-6:2005 standard, and conforms to the directives and other standards listed below: IEC 61010-1:2001, “Safety Requirements for Electrical Equipment for Measurement, Control, and...

  • Page 7: Character Formats And Symbols

    Pannoramic DESK II 1.23 User’s Guide Character Formats and Symbols Example Words or characters that appear on the screen. These include field names, screen and window titles, push-button and menu names, paths or options. Keys on the keyboard. For example, function keys (such as F11) or the Ctrl+M key combination.

  • Page 8: Notes Regarding Operational Safety

    The below section of this User’s Guide contains information and warnings of a kind that must be followed by owner/operator personnel. Warning and advisory symbols which are used on the base unit of the Pannoramic DESK II and in the section 1.1.3 Warning and Information Labels have the following meanings:...
  • Page 9 The front door is equipped with an interlock switch. If the door is opened while a Warning! digitization process is running, the process will be stopped instantly. A defective Pannoramic DESK II is not classified as domestic waste. It must be Warning! properly disposed in accordance with currently valid legal requirements.

  • Page 10: Notes On Warranty

    Pannoramic DESK II 1.23 User’s Guide Notes on Warranty We at Thermo Fisher Scientific are proud of our quality, reliability and of our after-sales service. We continuously strive to improve our service to our customers. Please ask a Thermo Fisher Scientific representative about Service Contracts which can keep your purchase in peak condition for many years to come.

  • Page 11: Terms And Abbreviations

    Pannoramic DESK II 1.23 User’s Guide Terms and Abbreviations Comma Separated Value Field of View Numerical Aperture Virtual Slide / Slide A digital image of a thin glass plate on which specimens are mounted for microscopic study: a dynamic, interactive image that you can manage, save, magnify, zoom, name, evaluate, annotate, mark and comment, send to a colleague electronically for co- operation or advice, and so on.

  • Page 12: Product Description

    1 Product Description 1.1 Product Overview 1.1.1 Intended Use Pannoramic DESK II is designed to use transmitted light to digitize biological samples and specimens (histological sections, typically). Warning! After each scanning make sure to inspect the digital slide for over-exposed areas.

  • Page 13: Warning And Information Labels

    Pannoramic DESK II 1.23 User’s Guide 1 Product Description 1.1.3 Warning and Information Labels The following figure contains the labels used on the Pannoramic DESK II main unit and the external mains power supply unit. Figure 1 – Warning and information labels NOTE: The symbol at the feeder slot calls attention to biohazardous materials in use.

  • Page 14: Base Unit

    Pannoramic DESK II 1.23 User’s Guide 1 Product Description 1.1.4 Base Unit The following figure shows the main parts of the base unit. Figure 2 – Main parts of the base unit 1. Protective cover 2. Sliding door 3. Scan camera June 4, 2018 –...

  • Page 15: System Overview

    The standard scope of delivery of the product includes the following hardware and software items. 1.2.1 Pannoramic DESK II system The following figure shows the main components of the Pannoramic DESK II system. Figure 3 – Pannoramic DESK II system overview 1.

  • Page 16: Hardware Options

    0.63× adapter 1× adapter Warning! The installation of the cameras in Pannoramic DESK II must be carried out by trained professionals! In any other case the warranty will be automatically voided. The supplier or distributor is not liable for any damages caused by an installation carried out by an unauthorized person.

  • Page 17: Control Software

    Pannoramic DESK II 1.23 User’s Guide 1 Product Description Base Unit options Pannoramic DESK II Brightfield Option – base unit equipped with Point Grey Grasshopper 3 • monochrome camera for Brightfield scanning Objectives Plan-Apochromat 20×/NA 0.8 • Plan-Apochromat 40× Corr/NA 0.95 •...

  • Page 18: Installation

    2 Installation 2 Installation Warning! The installation of Pannoramic DESK II must be carried out by a trained professional! In any other case the warranty will be automatically voided. The supplier or distributor is not liable for any damages caused by an installation carried out by an unauthorized person.

  • Page 19: Connectors And Cables

    Pannoramic DESK II 1.22 User’s Guide 2 Installation 1. Status display 2. DVD drive (RW) 3. Power on/off button 4. Earphone and microphone 3.5mm Jack ports 5. 2x USB port 6. Power supply connector 7. PS/2 mouse port 8. PS/2 keyboard port 9.

  • Page 20: Preparing Slides

    Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides 3 Preparing Slides You can use all large slides and cover slips that meet the following specifications: Slides Cover slips Length 75.0 to 76.0 mm max. 50 mm Width 25.0 to 26.0 and max.

  • Page 21: Affixing Barcode Stickers To Slides

    Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides 3.1 Affixing Barcode Stickers to Slides This section describes how to affix barcode stickers to large slides. Barcodes enable virtual slide identification. Figure 5 – Normal and large slides with barcode stickers The label area (1) is recorded by the preview camera, and can be displayed by the control and viewer software.

  • Page 22: Inserting And Removing Slides

    Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides 3.2 Inserting and Removing Slides Pannoramic DESK II features a manual feed for one single slide (normal or large). Caution! An adapter must be placed onto the specimen holder if normal slide is fed into the device.
  • Page 23 Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides If a large slide is needed to be fed and the adapter is attached to the specimen holder, it must be removed first before feeding the slide. 1. Open slide feeder door, and locate the adapter (marked with red on Figure 7) at the front edge of the specimen holder Figure 7 –...
  • Page 24 Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides 4. The specimen holder is now capable to receive a large slide Figure 9 – Specimen holder with the adapter removed If you want to load normal slide into the device, and the adapter is not placed on the specimen holder, follow the below steps: 1.
  • Page 25 Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides 4. After the adapter is placed on its location, lift the applicator and make sure the adapter stays at its place Figure 11 – Lifting off the applicator Note: The device detects the presence of the adapter, and therefore preview image area is automatically modified accordingly.
  • Page 26 Broken slide can be removed only by a well-trained person! Figure 12 – Inserting and removing a slide 1. Click in Pannoramic DESK II control software. The specimen holder moves to the load/unload position. 2. Open the slide insert door (1) by pushing it to the left.
  • Page 27 Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides The cover slip and the label area should be on the top, with the label area pointing towards the slide insert door. Warning! To avoid jamming or breaking slides, ensure that the slide is not tilted and that it rests perfectly horizontally on the specimen holder.
  • Page 28 Pannoramic DESK II 1.23 User’s Guide 3 Preparing Slides 1. Click in Pannoramic DESK II control software. The specimen holder moves to the load/unload position 2. Open the slide insert door (1) by pushing it to the left. 3. Open the fixing lever by pulling it to the left.

  • Page 29: Software Description

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4 Software Description 4.1 Starting / Exiting Pannoramic DESK II Software Application 4.1.1 Starting the software At the startup of the program you can select the manual scanning mode for the scan software.
  • Page 30 Pannoramic DESK II 1.23 User’s Guide 4 Software Description June 4, 2018 - Rev. 2 3DHISTECH Ltd. 30(82)
  • Page 31 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Figure 15 – Barcode settings Barcode settings can be modified in the Barcode Settings window. By predefining the barcode type, foreground color (black or white – the color of the barcode), and other settings, you enable the Read Barcode option when using the control software.
  • Page 32 Pannoramic DESK II 1.23 User’s Guide 4 Software Description You can only scan the barcode type that you have previously set. If you want to read several barcode types, you always have to set the current barcode type before reading it.
  • Page 33 Pannoramic DESK II 1.23 User’s Guide 4 Software Description 1D Barcode Types You can select the following 1D barcode types: *Code 39 • Code 128 • Code 16K • *Interleaved 2 of 5 • Standard 2 of 5 • IATA 2 of 5 •...

  • Page 34: Image Compensation

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description Exclude check digit (default is ON) If activated, the check digit value (last digit of the barcode), an additional checking number of the barcode will also be analyzed as a barcode number.
  • Page 35 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Brightfield Compensation Figure 16 – Brightfield Compensation Camera Selection This field displays the active camera. Currently, there is only one active camera for Brightfield mode. Objective Selection The installed objective is selected for creating a new compensation image.
  • Page 36 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Image Browser panel Figure 17 – Image Browser This function displays the created compensation image. June 4, 2018 - Rev. 2 3DHISTECH Ltd. 36(82)

  • Page 37: Microscope Settings

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.2.3 Microscope Settings By moving the mouse over the buttons, the software displays information about the setting you are interested in. Base Settings Figure 18 – Base settings Objective type You can select the type and the magnification level of the objectives.
  • Page 38 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Brightfield ICC Profile Select an applicable preset color profile to be used for Brightfield scanning from the drop-down menu. Settings according to the profile has effect on the color set, color temperature, and gamma value of the scanned image.
  • Page 39 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Filter Settings Figure 19 – Filter settings The settings on this panel apply to Fluorescent mode, therefore irrelevant and inactive. June 4, 2018 - Rev. 2 3DHISTECH Ltd. 39(82)
  • Page 40 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Camera Rotation Figure 20 – Camera rotation Caution! If you have transported the product, or changed the camera, there is a possibility, that the camera does not display the images properly. If you experience such problems, contact service support.

  • Page 41: Event Call Settings

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.2.4 Event Call Settings Figure 21 – Event Call Settings By moving the mouse cursor over the buttons, the software displays information about the setting you are interested in. Two of the three settings are responsible for opening external applications (.exe or .bat) as defined (for example, Pannoramic Viewer software can be opened after scanning is...
  • Page 42 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Run defines the window mode that the external application will start in. The following window modes can be set: Normal window • Minimized • Maximized • You can test whether the Scanning Error Occurred settings work by clicking In the Message Call section of the window complex messaging functions can be defined.
  • Page 43 Pannoramic DESK II 1.23 User’s Guide 4 Software Description In the Messenger Setup window the following parameters can be set: Message Type – E-mail (SMTP) • Host Address • Authentication – User ID and User Password are required. • Secure connection (SSL) – If activated, the messages will be sent through secured •...

  • Page 44: Using Brightfield Manual Mode

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.3 Using Brightfield Manual Mode The Brightfield Manual mode is perfect for digitizing slides that require unique settings, or single slides. You can use this mode to set the scanning, preview, and image quality settings. You can also set profile settings here, and start scanning in Brightfield Manual mode.

  • Page 45: Routine Work Tab

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.3.1 Routine work tab Figure 25 – Routine work tab The Routine work tab provides the following basic digitization functions: To insert and remove slides To move the specimen holder to load/unload position, click •...
  • Page 46 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Set server parameters Set connection to the dedicated CaseCenter server. See section 4.4 Scan to Server for more • information. Output resolution The resolution of the output digital slide can be modified to result a smaller file size. You can •...

  • Page 47: Scan Options Window

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.3.2 Scan Options window Figure 26 – Scan options window These settings will determine the resulting quality and storage location of the virtual slide. You can choose the following image formats in the Image Encoding field: JPEG –...
  • Page 48 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Scan Settings Focus distance in field-of-views – the distance of focal points can be set to create a focus • map, based on which the software calculates interpolated focus values (one step in distance means one FOV).

  • Page 49: Preview Tab

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.3.3 Preview tab The Preview tab offers options for threshold setting and selection of the area to be scanned. Figure 27 – Preview tab Use threshold Scan specimen with auto threshold – This automatically detects the specimen in the preview •...
  • Page 50 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Scan specimen with user set threshold – This sets the sensitivity of the specimen detection. • To set the desired threshold value manually, change the setting in the Specimen threshold level selection box in the range of 0-255. The default value is 35.
  • Page 51 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Remove coverslip – If selected, the edges of the coverslip are not detected as specimen to • be scanned. Depending on the threshold level, it is possible that the edges of the coverslip are detected as areas to be digitized.

  • Page 52: Preview Area Scanned By Preview Camera

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description Use saved scan area This function is useful if the slides contain areas of interest that cannot be detected by the preview camera. This function along with saving profiles helps to scan all slides with the most adequate settings.
  • Page 53 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Areas that will be scanned These areas are digitized on the basis of user settings (for example, Fill holes in scan area) and not detected by the selected threshold value. Detected sample areas that will be scanned These areas are digitized.
  • Page 54 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Navigation Tool: This tool offers easy and punctual slide navigation in distinctive steps. Set Step Size (FoV) – 1, ½, or ¼ to determine the size of movement in proportion of one FOV. Click an arrow to move to the required direction with the set step size.
  • Page 55 Pannoramic DESK II 1.23 User’s Guide 4 Software Description To select an existing TMA object, click on the object name. The selected object • name will highlight. To delete an existing TMA object, click Delete. • Block tab Figure 35 – TMA tool: Block Set the type of TMA block on this panel.
  • Page 56 Pannoramic DESK II 1.23 User’s Guide 4 Software Description The Move tab allows the rotation and moving of the TMA selection spots that are displayed on the preview image. To move the spots enter the pixel value in the Move field or use the arrows.

  • Page 57: Focus Tab

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.3.5 Focus tab Figure 39 – Focus tab Live To display a live image of the current specimen in the Live image display field, click . It will work only if you select an area in the preview image with the Position sample.

  • Page 58: Service Tab

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description for example, one current, two focus levels above and below FOVs are captured. Therefore, it is advised to choose an odd number for the focus levels. Focus step size determines the spacing between focus levels. One step is around 0.2 µm.
  • Page 59 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Figure 42 – Display field and toolbar for live images Navigation on the live image Click a desired area of the slide in the Live image to display that area in the center of the Live image field.

  • Page 60: Start Scan

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description – Zoom in • – Zoom out • – One pixel on the monitor corresponds to one pixel on the camera • – The live image completely fills the display area •...

  • Page 61: Scan To Server

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description Caution! Before starting digitization process ensure that you have enough free space on the destination drive. You can check the available free disk space in the last line (Available disk space) of the information area of Scan Progress.
  • Page 62 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Figure 44 – Scan to CaseCenter option 3. Type in or select the server address from the Connect to: drop-down list, and give the User name and Password. Activate the Secure connection option to enable HTTPS connection. Click Connect when finished.
  • Page 63 Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4. Click the Connect button in the Scan Options window to connect to the server. If the connection is established the server status will be modified from Not connected to Connected.
  • Page 64 Pannoramic DESK II 1.23 User’s Guide 4 Software Description Use masks for automatic fills. Activate the Use mask option and the mask fields turn • fillable. To save masks, click the Save Mask button, then fill the slide data fields. To modify masks click Set Mask.
  • Page 65 Pannoramic DESK II 1.23 User’s Guide 4 Software Description To erase/reset fields to default, click the Reset all button. • If modification is done to the folder tree of the CaseCenter server while the Scan To • Server window is open, click Update Tree to refresh the tree view.

  • Page 66: Using Profiles

    Pannoramic DESK II 1.23 User’s Guide 4 Software Description 4.5 Using Profiles Note: Saved profile settings cannot be applied if using a different type of Pannoramic scanner. The following settings are saved in a profile: Scan Mode Brightfield • Scan Options Focus frequency •...

  • Page 67: Troubleshooting And Maintenance

    Pannoramic DESK II 1.23 User’s Guide 5 Troubleshooting and Maintenance 5 Troubleshooting and Maintenance 5.1 Safety Information Check cables, plugs and connections before operation, and if defective or cannot be replaced, contact Thermo Fisher Scientific Service for support. Check whether the device is compatible with your local line voltage. It can operate at line voltages 100V to 240V and 50/60 Hz.

  • Page 68: Troubleshooting

    • 1. Exit the application software. 2. Switch off the power supply and disconnect the power cable. 3. Remove the protective cover of the Pannoramic DESK II. Follow the steps in section 5.3.1 Removing the protective cover. 4. Remove slide manually (see section 5.3.2 Removing a...
  • Page 69 Pannoramic DESK II 1.23 User’s Guide 5 Troubleshooting and Maintenance Imaging error (for example, chromatic aberrations) • Contact Thermo Fisher Scientific Service for support. • Warning! Do not modify the camera driver settings, as the default factory settings give the most accurate images.

  • Page 70: Maintenance

    Pannoramic DESK II 1.23 User’s Guide 5 Troubleshooting and Maintenance 5.3 Maintenance This section describes the most common maintenance solutions that can be performed by the user of the product. For maintenance solutions not described in this manual, contact the service personnel.

  • Page 71: Removing A Slide

    Pannoramic DESK II 1.23 User’s Guide 5 Troubleshooting and Maintenance 1. Remove both Allen screws (2) located at the left and right sides of the cover (1) by using an Allen screwdriver (3). 2. Carefully push the cover (5) horizontally frontward with light pressure until it can be removed completely from the basic unit (4).
  • Page 72 Pannoramic DESK II 1.23 User’s Guide 5 Troubleshooting and Maintenance Figure 53 – Removing a slide 1. Exit control software. 2. Switch off power supply and disconnect power cable. 3. Remove the cover (see section 5.3.1 Removing the protective cover ).

  • Page 73: Transporting Pannoramic Desk Ii

    5.3.3 Transporting Pannoramic DESK II The following procedure shows how to transport Pannoramic DESK II. Caution! Do not lift or transport Pannoramic DESK II by holding its protective covers! Note: The base unit does not have carrying handles for transporting. One or two persons can carry the base unit by holding it by its base plate.

  • Page 74: Technical Data

    Pannoramic DESK II 1.23 User’s Guide 6 Technical Data 6 Technical Data Dimensions (width x depth x height) Base unit approx. 380 mm (W) x 306 mm (D) x 249 mm (H) 24V 100W power supply approx. 189 mm x 90 mm x 44 mm...
  • Page 75 Pannoramic DESK II 1.23 User’s Guide 6 Technical Data Operating data Intended site closed room facilities Electrical protection class Internal protection degree IP 20 Electrical safety in compliance with DIN EN 61010-1:2001, DIN EN 61010-2- 101:2002 (IEC 61010-1:2001, IEC 61010-2-101:2002)
  • Page 76 Pannoramic DESK II 1.23 User’s Guide 6 Technical Data Slide identification Slides are identified by a VRmagic VRmC-8+ PRO preview camera. Label area is saved as an image and the barcode is interpreted as a character string. Scan camera Point Grey Grasshopper 3 (GS3-U3-51S5M-C)

  • Page 77: Appendix - Packaging Instructions

    Pannoramic DESK II 1.23 User’s Guide Appendix – Packaging instructions Appendix – Packaging instructions Figure 1. Preparations 1. Unload the slide from the device by using the scanner software. Remove your slide. 2. Close the scanner software normally. 3. Turn off the scanner with the main switch located on the backside of the device. Disconnect all the cables of the scanner.

  • Page 78: Packaging

    Pannoramic DESK II 1.23 User’s Guide Appendix – Packaging instructions 3. BOX 3 – carton box dedicated for the PC a) PC (placed inside the dedicated foam) b) power cable for PC c) power cable for scanner d) USB cable of the scanner e) keyboard and mouse (has a separated small carton box) 4.
  • Page 79 Pannoramic DESK II 1.23 User’s Guide Appendix – Packaging instructions 1. BOX 2 – scanner box 2. Fixing foams for underneath BOX 2 3. Fixing foams for above BOX 2 4. Bottom part of BOX 1 5. Top of BOX 1 6.

  • Page 80: Packing The Desk Ii Scanner

    Pannoramic DESK II 1.23 User’s Guide Appendix – Packaging instructions Packing the DESK II scanner The most sensitive and most heavy unit is the DESK II scanner. Please handle it with care, the weight of the device is about 20-22 kg.

  • Page 81: Handling Of The Package

    Pannoramic DESK II 1.23 User’s Guide Appendix – Packaging instructions Handling of the package Please be aware to observe the following instructions on Figure 5.: Designation Symbol Explanation The package must always be transported, handled and stored in such a way that the arrows always point upwards.

  • Page 82: Index

    Marker..........................49, 52, 67 Pannoramic............................6 Pannoramic DESK II........6, 8, 9, 10, 12, 13, 15, 16, 17, 18, 22, 26, 28, 29, 69, 71, 74 Pannoramic Viewer......................17, 58, 69, 70 Preview............19, 21, 27, 29, 34, 43, 48, 50, 51, 52, 53, 55, 56, 58, 77 RGB..............................61...

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