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thermo spectronic GENESYS 10 Operator's Manual

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GENESYS™ 10 Series
Spectrophotometers
Operator's Manual

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Summary of Contents for thermo spectronic GENESYS 10

  • Page 1 GENESYS™ 10 Series Spectrophotometers Operator’s Manual...
  • Page 2 Copyright © 2002, Thermo Spectronic All rights reserved.
  • Page 3 Connecteur courant A/C Stromanschluss Connettore alimentazione CA RS232C port Salida RS232C Port RS232C Figure 2a Back panel of the GENESYS 10 Vis and RS232C Port GENESYS 10 UV spectrophotometer Porta RS232C Panel posterior del espectrofotómetro Analog/BNC connector Panneau arrière du spectrophotomètre Conector analogo Rückwand des Spektrophotometer...
  • Page 4 Conector C/A Connecteur courant A/C Stromanschluss Connettore alimentazione CA Parallel port Puerto Paralelo Port parallèle Figure 2b Back panel of the GENESYS 10 UVvscanning spectrophotometer Parallelanschluss Panel posterior del espectrofotómetro Porta parallela Panneau arrière du spectrophotomètre RS232C port Rückwand des Spektrophotometer...
  • Page 5 La tastiera sullo spettrofotometro GENESYS™ 10 Figure 5 Installing the Single-Position Cell Holder (335916) Instalando el portaceldas sencillo (335916) Installation du porte-cuves à emplacement unique (335916) Installieren des Einfach-Kuevettenhalters (335916) Installazione del supporto cella a posizione singola (335916) GENESYS 10 Operator’s Manual...
  • Page 6 Aligning the lamp assembly Replazando la lámpara de tungsteno Alineando el ensamble de la lámpara Remplacement de la lampe à tungstène Alignement du bloc lampe Ausbau der Wolframlampe Justieren der Lampe Sostituzione della lampada incandescente Allineamento del portalampada GENESYS 10 Operator’s Manual...
  • Page 7 Removiendo el porta fusible Enlèvement du porte-fusibles Entfernen des Sicherungshalters Rimozione del portafusibile Figure 10 Removing and replacing the fuses Removiendo y reemplazando los fusibles Enlèvement et remplacement des fusibles Tauschen der Sicherungen Rimozione e sostituzione dei fusibili GENESYS 10 Operator’s Manual...
  • Page 8 GENESYS 10 Operator’s Manual...
  • Page 11 However, as part of Thermo Spectronic's on-going program of product development, the specifications and operating instructions may be modified or changed from time to time. Thermo Spectronic reserves the right to change such operating instructions and specifications. Under no circumstances shall Thermo Spectronic...
  • Page 12 UPDATE POLICY Thermo Spectronic may, from time to time, revise the performance of its products, and in doing so incur no obligation to furnish any such revisions to any Thermo Spectronic customer. GENESYS 10 Operator’s Manual...
  • Page 13 SOFTWARE PASSWORD This password allows you to enter the security section of the software used on the Thermo Spectronic spectrophotometer. Through the security section, you can "lock" test setups (test parameters) so that they may not be altered. The password also allows you to remove the security so that you may edit the test parameters.
  • Page 14 GENESYS 10 Operator’s Manual...

  • Page 15: Table Of Contents

    Recalling a test...........................2-9 Setting up test parameters ......................2-9 Measuring a standard......................2-10 Entering a factor........................2-10 Measuring unknowns.......................2-11 Standard Curve..........................2-11 Recalling a standard curve......................2-11 Setting the parameters for a standard curve................2-11 Measuring the standards for a standard curve ..............2-12 GENESYS 10 Operator’s Manual...
  • Page 16 Wavelength Accuracy - Standards ....................3-2 Adding wavelengths........................3-2 Deleting wavelengths.........................3-3 Photometric Accuracy ........................3-3 Selecting the mode ........................3-3 Adding standards ........................3-3 Deleting standards ........................3-4 Running the test.........................3-4 Noise Measurement.........................3-4 Stray Light ............................3-5 Running the test.........................3-5 Internal Printer Test ..........................3-5 GENESYS 10 Operator’s Manual...
  • Page 17 Cleaning and maintenance of cells...................5-1 Cleaning the windows of the sample compartment..............5-2 Changing the fuse ..........................5-2 Replacing the tungsten lamp......................5-2 Replacement parts ...........................5-3 Appendix A - Specifications Appendix B - Parameters Appendix C - Calculations Index GENESYS 10 Operator’s Manual...
  • Page 18 Table of Contents viii GENESYS 10 Operator’s Manual...

  • Page 19: Chapter 1 - Setting Up The Instrument

    Calibrating grating Setting up the instrument 9. For the GENESYS 10 Vis unit only: When the 1. Carefully unpack the shipping carton and verify power-on sequence is completed, allow the that you have received all the items listed lamp to warm up for 30 minutes before taking below.

  • Page 20: Setting Utility Parameters

    15 minutes. Since the tungsten lamp of the GENESYS Vis spectrophotometer requires a 30- Setting the date and time minute warm-up, your GENESYS 10 Vis To access the date and time settings: spectrophotometer has been pre-set at the factory •...

  • Page 21: Setting Baseline Expiration Time

    • Parallel port to an HP PCL formatted printer • Enter the desired time into the Entry baseline (only available on the GENESYS 10 expiration time field. Press ENTER. UVscanning model only - catalog numbers Setting the screen contrast 335906 and 335907).

  • Page 22: Correcting For Cell Variability

    Although it would be ideal to use exactly the same cell for every reading, it is usually inconvenient to clean cells between readings. The GENESYS 10 UVscanning model uses an automated program to correct for cell variability, allowing you to use up to six unmatched cuvettes.

  • Page 23: Chapter 2 - Using The Instrument

    In addition to these units, you can create your own 1. After setting up the values for the test custom unit, using a character list like the one parameters, press Save Test. The described in Specifying names for tests. Once Create Test Name screen appears. GENESYS 10 Operator’s Manual...

  • Page 24: Using The Smartstart Feature

    Using the SmartStart feature (GENESYS 10 UVscanning model only) 1. With the list of basic units displayed, use the arrow keys to highlight [Unit] and press ENTER. The SmartStart feature enables you to select the test methods you use most frequently and have them appear when you start up your instrument.

  • Page 25: Running The Cell Correction Program

    When it starts up again, the list of tests you’ve selected will be displayed. Running the cell correction program (GENESYS 10 UVscanning model only) Note: The Cell Correction program is not active in the Main (Basic Absorbance/%T/Basic Concentration) screen.
  • Page 26 Cell Correction program will be run. A list of wavelengths appears. 5. If you selected Scan mode in the preceding step, press Run Corr. to start the Cell Correction program. If you selected Discrete nms mode, first specify the wavelengths using GENESYS 10 Operator’s Manual...

  • Page 27: Taking Measurements

    Once you select the values for the parameters, you This process is completely manual. In fact, the GENESYS 10 Operator’s Manual...

  • Page 28: Basic Absorbance/%T Measurements

    %T and you use the Change Mode function key to switch to concentration measurements. The spectrophotometer allows you to measure concentration using either a factor or a standard to convert absorbance readings to concentration units. GENESYS 10 Operator’s Manual...

  • Page 29: Setting The Wavelength & Mode

    1. If necessary, press Change Mode to switch to the Concentration with Standard mode. 6. Press Measure Standard to measure the standard. When the instrument is finished measuring the absorbance of the standard, it displays the absorbance and calculated factor. GENESYS 10 Operator’s Manual...

  • Page 30: Entering A Factor

    • Select the measurement mode you want to use (Absorbance or %Transmittance) • Run Cell Correction program (GENESYS 10 UVscanning model only) • Recall a test OR set up your test parameters 3. Use the arrow keys to highlight the entry in the •...

  • Page 31: Setting Up Test Parameters

    • Set up test parameters Taking measurements manually (using Manual • Run Cell Correction program (GENESYS 10 6 or Single Cell Platform) UVscanning model only) 1. With the Advanced A/%T/C screen displayed • Save a test and the parameters set, press Measure •...

  • Page 32: Measuring A Standard

    Run Standard. The Measure Standard screen appears. 2. Use the number keys to enter the concentration value of the standard and press ENTER. 3. Place the blank and standard in the correct cell positions. 2-10 GENESYS 10 Operator’s Manual...

  • Page 33: Entering A Factor

    Measurement Mode set to Conc/Std or Conc/Factor, press Run Test. The Advanced A/%T/C measurement screen appears. 2. Place the blank and unknown in the correct cell position. If your instrument is equipped with a GENESYS 10 Operator’s Manual 2-11...

  • Page 34: Recalling A Standard Curve

    4. When you have entered all the concentration values, the Standards screen appears. • Run the Cell Correction program (GENESYS 10 UVscanning model only) Measuring the standards for a standard curve Measuring standards automatically (using Auto 6 or Auto 3) 1.

  • Page 35: Measuring Unknowns

    Standard Curve screen appears, showing the absorbance and concentration of each unknown. If you want to switch between tabular and graphical displays, press View Graph/Tabular. GENESYS 10 Operator’s Manual 2-13...

  • Page 36: Absorbance Ratio

    2. Use the arrow keys to highlight Delete can perform these tasks: Standard. Press ENTER to delete the • Recall a test OR set up your test parameters standard. • Run the Cell Correction program (GENESYS 10 UVscanning model only) 2-14 GENESYS 10 Operator’s Manual...

  • Page 37: Recalling A Test

    1. With the Absorbance Ratio screen displayed, press Run Test. From this screen, you can: • Set up test parameters • Run the Cell Correction program (GENESYS 10 UVscanning model only) • Save a test • View the list of stored tests • Measure a blank •...

  • Page 38: Absorbance Difference

    Set up test parameters position. If your instrument is equipped with a 6-Position Cell Holder, you can place up to five • Set up Cell Correction program samples in the cell holder. (GENESYS 10 UVscanning model only) 2-16 GENESYS 10 Operator’s Manual...

  • Page 39: Kinetics

    Save Test to save the test or Run Test to measure only one unknown at a time. measure a blank or an unknown. Note: The Kinetics program allows you to collect up to 400 data points per run. When you GENESYS 10 Operator’s Manual 2-17...

  • Page 40: Measuring Unknowns

    When your results are displayed on the screen, you can modify the range (start and 2-18 GENESYS 10 Operator’s Manual...

  • Page 41: Rescaling & Recalculating Tabular Kinetics Results

    The instrument displays the data for the 2. Press Start Time or End Time. The instrument selected point. displays the recalculated rate and result. GENESYS 10 Operator’s Manual 2-19...

  • Page 42: Survey Scan/Scanning

    Collect a baseline • Scan unknowns The Survey Scan/Scanning program allows you to scan a wavelength range. The GENESYS 10 Setting up test parameters UVscanning model supports full range scanning 1. With the Survey Scan/Scanning screen (190-1100nm); GENESYS 10 Vis and UV models displayed, use the arrow keys to highlight the can scan a range of up to 100nm only.

  • Page 43: Scanning An Unknown

    Edit Graph before you can perform other functions on the scan data. When the edit graph screen appears, you can: • Rescale the graph • Perform mathematical operations on the graph GENESYS 10 Operator’s Manual 2-21...
  • Page 44 (designated for the left, center and right wavelengths) appears. 2. To set the minimum or maximum value for the X- or Y-axis, press Min Y, Max Y, Min X Max X. A screen appears prompting you to enter the appropriate value. 2-22 GENESYS 10 Operator’s Manual...
  • Page 45 3. Use the arrow keys to select the type of labels you want displayed and press ENTER. The instrument labels the selected items on your scan data plot. Note: Up to nine peaks or valleys can be calculated and displayed. GENESYS 10 Operator’s Manual 2-23...

  • Page 46: 3-Point Net

    1. With your table of scan data displayed on the • Run the Cell Correction program edit screen, use the arrow keys on the keypad (GENESYS 10 UVscanning model only) to move the highlight symbol (→ → ) to the appropriate data point in the table. •...

  • Page 47: Multiple Wavelengths

    3. Press Measure Blank to measure the blank. If the screen. your instrument is equipped with a 6-Position From this screen, you can: Cell Holder, it automatically moves to the B position to measure the blank. When it • Set up test parameters GENESYS 10 Operator’s Manual 2-25...

  • Page 48: Setting Up Test Parameters

    Using the Instrument • Run the Cell Correction program 4. Enter the values for the wavelength and factor (GENESYS 10 UVscanning model only) and press ENTER. • Save a test 5. When the values are correct, press Add nm. •...
  • Page 49 6-Position Cell Holder, press the cell position buttons on the keypad to reposition the cell holder and measure the rest of the unknowns manually. GENESYS 10 Operator’s Manual 2-27...
  • Page 50 Using the Instrument 2-28 GENESYS 10 Operator’s Manual...

  • Page 51: Chapter 3 - Using The Performance Validation Program

    • UV-Vis instruments - measures wavelength accuracy for the xenon lamp at 229, 529 and 883nm. • Vis instruments - measures wavelength accuracy for the internal didymium filter at 585 and 807nm. GENESYS 10 Operator’s Manual...

  • Page 52: Wavelength Accuracy - Standards

    SPECTRONIC Standards wavelength standard is certified. The wavelength accuracy 4. When the value is correct, press Add test passes or fails based on this wavelength Tolerance to add the tolerance. alone. The other two wavelengths may be GENESYS 10 Operator’s Manual...

  • Page 53: Deleting Wavelengths

    %transmittance) of a set of standards and compares the results with specified tolerances. The absorbances and tolerances are preset, but you should change them to the values on the report of calibration included with your standards. GENESYS 10 Operator’s Manual...

  • Page 54: Noise Measurement

    2. Press Start Test to run the test. The results of Noise Measurement. the test appear on the screen, indicating pass 2. Press ENTER. The Noise Measurement or fail for each wavelength. screen appears. GENESYS 10 Operator’s Manual...

  • Page 55: Internal Printer Test

    Position #3 should be used for SRE 400. 2. With the Performance Validation screen You may use other filters, but they must have displayed, use the arrow keys to highlight < – 0.1%T at the wavelength of interest. Internal Printer Test. GENESYS 10 Operator’s Manual...

  • Page 56: Rs232C Test

    Stop Test to 2. With the Performance Validation screen stop it. displayed, use the arrow keys to highlight If the test fails, contact your distributor for technical RS232C Test. support. GENESYS 10 Operator’s Manual...

  • Page 57: Chapter 4 - Connecting & Using Accessories

    Peristaltic pump, 335171 Optical glass flowcell, 335916 Single cell platform, 335920 Accessory door kit and pump tubing, 110 VAC. 335976* Sipper System - Same as 335975 except for measurements in the 190-1100 wavelength range, 110 VAC. GENESYS 10 Operator’s Manual...

  • Page 58: Changing Cell Holders

    3. With the other hand, pull straight up on the 6- 2. Tighten the screws securely. Position Cell Holder and lift it out of the sample compartment. Note: If the cell holder is not aligned correctly, you will not be able to tighten the screws. GENESYS 10 Operator’s Manual...

  • Page 59: Internal Printer

    2. Install the desired accessory cell holders by External Printers tightening the screw on each cell holder. GENESYS 10 models series 335906 and 335907 Note: You can install only three each of some are able to print to external desktop printers accessory cell holders.
  • Page 60 Connecting & Using Accessories GENESYS 10 Operator’s Manual...

  • Page 61: Chapter 5 - Performing Maintenance Procedures

    (with stirring) 800mL of concentrated sulfuric acid to 458mL of distilled water containing 92 g of sodium dichromate (Na •2H O). This cleaning solution should be red-brown. Discard, using proper disposal methods, when green tinge appears. GENESYS 10 Operator’s Manual...

  • Page 62: Cleaning The Windows Of The Sample Compartment

    Changing the fuse Replacing the tungsten lamp The fuse is located in the power entry module located at the center of the back panel of the (for GENESYS 10 VIS spectrophotometers only) instrument (Figure 2). • 120VAC, 2.5A, Slo-Blo •...

  • Page 63: Replacement Parts

    Service Manual, includes printed service manual and CD ROM. 335901-10040 GENESYS 10 Service Manual, includes CD ROM only. 335971 Replacement silicon tubing for 335982 Peristaltic pump, 5mm OD, 1.5mm ID, 25 foot roll. 335923 Tungsten Lamp Assembly - For all GENESYS 10 Vis spectrophotometers. GENESYS 10 Operator’s Manual...
  • Page 64 Performing Maintenance Procedures GENESYS 10 Operator’s Manual...

  • Page 65: Appendix A - Specifications

    Appendix A - Specifications Specifications Table 1 Specifications for GENESYS 10 spectrophotometers GENESYS 10 Vis GENESYS 10 UV GENESYS 10 UV Scanning Spectral Bandwidth Optical System Single-beam, grating- Split-beam, grating-based, based, single detector dual detectors Lamp Source; Tungsten-halogen; Xenon; 5 years typical...
  • Page 66 24 hours after taking it out of storage. Temperature should be maintained at ±4° F. Relative humidity should be maintained to ±5%. Altitude From below sea level to 2000 meters (6562 feet) For indoor use only Installation Category II Pollution Degree 2 GENESYS 10 Operator’s Manual...

  • Page 67: Appendix B - Parameters

    Number of samples to be measured in the whole test Number of Standards Number of standards to be measured for standard curve Phot Accy Tolerance ±Photometric accuracy acceptable Pk-Pk Noise Tolerance ±Noise acceptable Printer Selects output mode GENESYS 10 Operator’s Manual...
  • Page 68 OFF, and/or when test parameters are changed, and/or when test is saved (or resaved) Std Concentration Concentration of analyte in standard solution Stop Wavelength Ending wavelength for a scan Test Name User-defined name for test GENESYS 10 Operator’s Manual GENESYS 10 Operator’s Manual...
  • Page 69 The segmented model requires: Segmented model • Data for at least two standard data points with different concentrations and absorbances • Slopes of all segments must be ascending (positive) OR descending (negative) GENESYS 10 Operator’s Manual...
  • Page 70 − Abs Absλ Absλ Absorbance Difference ∗ factor 2 Result = Absλ * factor 1 - Absλ (Absλ - Absλ ) * factor 1 - (Absλ - Absλ ) * factor 2 GENESYS 10 Operator’s Manual GENESYS 10 Operator’s Manual...
  • Page 71 Appendix C - Calculations Calculation Calculation(s) Graphs Baseline corrected absorbance = 3-Point Net λ  λ   A λ  λ 3-Point Net Absorbance sample curve GENESYS 10 Operator’s Manual...
  • Page 72 Appendix C - Calculations GENESYS 10 Operator’s Manual...
  • Page 73 Cleaning ..... . .5-1, 5-2 rescaling & recalculating results ..Concentration measurements ..2-6, 2-10 2-18, 2-20 Concentration units ..2-1, 2-2, 2-6, 2-7 GENESYS 10 Operator’s Manual...
  • Page 74 2-20, 2-21, 2-24, 2-25, 2-26, A-1 Utility parameters, setting ..1-2, 1-3 Screen contrast, setting ....1-3 GENESYS 10 Operator’s Manual...
  • Page 75 ..... . .3-2 Z-dimensions ..... . .1-2 GENESYS 10 Operator’s Manual...
  • Page 76 Index GENESYS 10 Operator’s Manual...
  • Page 77 THERMO SPECTRONIC (Asia Pacific, Middle East, Africa, India and Latin America) Contact Rochester, New York, USA SPECTRONIC is a registered trademark and GENESYS is a trademark of Thermo Spectronic. Thermo Spectronic is an ISO 9001 Systems Certified Company. Themo Electron Spectroscopy...

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