Summary of Contents for Antec ROXY
- Page 1 Antec Scientific Industrieweg 12 2382 NV Zoeterwoude The Netherlands ROXY Installation Guide 210.7011, Edition 5, 2018 T +31 71 5813333 info@AntecScientific.com www.AntecScientific.com...
- Page 2 (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from the copyright of the owner. Antec reserves the right to make changes to the design and speci- fications of the instrument and this manual without prior notice.
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Page 3: Table Of Contents
IQ and OQ ..................... 11 PQ ......................11 Relevant documents for installation ............13 Software download ................13 Contents of ROXY ................. 14 Installation ....................15 Electrical connections ................16 Software installation ................18 Installation of a flow cell ................ 20 Installation of grounding kit .............. -
Page 4: Warning Symbols
4 (of 33) ROXY installation guide, ed. 4 Warning Symbols The following symbols are used in this guide: This sign warns about the risk of electric shock. It calls attention to a procedure or practice which, if not adhered to, could result personal injury or even loss of life by elec- trocution. - Page 5 Installation guide 5 (of 33) The attention sign signals relevant information. Read this information. The note sign signals additional information. It provides advice or a suggestion that may support you in using the equipment.
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Page 6: Intended Use
In Vitro Diagnostics Directive. Operation of the ROXY EC(/LC) system can involve the use of hazardous materials including corrosive fluids and flammable liquids. The system should only be operated by users with the following expertise: •... -
Page 7: Weee Directive
Else the protective and safety equipment of the device could fail. The ROXY EC system is ROHS compliant and in conformity with Directive 2011/65/EU Restricted use of Hazardous Substances in electrical and electronic Equipment (ROHS). -
Page 8: Warranty, Spare Parts And Service
ROXY installation guide, ed. 4 Warranty, spare parts and service The warranty period of this ROXY EC system is 1 year on workmanship, wear and tear parts are excluded. Manufacturer provides operational spare parts of the instrument and current accessories for a period of five years after shipment of the final production run of the flow cell. - Page 9 When the system or parts of it needs to be shipped to Antec for service/repair or dis- posal, it has to be accompanied with a decontamination form which should be completely filled in and signed by the customer.
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Page 10: Introduction
Fig. 1. ROXY EC/MS with syringe pump for continuous flow experiments. This document is intended as a general check list to guide you through the installation of a ROXY EC system and by no means a replacement of the installation sections in the individual user manuals supplied with... -
Page 11: Preparing For Installation
ROXY EC system. The ‘site preparation reply form’ in the document confirming that all requirements are met, has to be signed and returned by the user prior to installation. - Page 12 Amodiaquine and Insulin, respectively. Which test is required is depending on the specific ROXY system installed and/or customers application (OX/RED). A PQ should be performed as last step of the installation a to assure the proper operation of the full ROXY EC system (Syringe pump, ROXY poten- tiostat, flow cell) in combination with MS.
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Page 13: Relevant Documents For Installation
Installation guide 13 (of 33) Relevant documents for installation The detailed description of ROXY EC system installation is part of the fol- lowing user manuals: 210.7050 - ROXY Installation Requirements (prior to installation!) 210.7011 - ROXY EC Installation guide (this document) 210.7010 - ROXY Potentiostat user manual... -
Page 14: Contents Of Roxy
14 (of 33) ROXY installation guide, ed. 4 Contents of ROXY Although there are a few exceptions, most ROXY EC systems are deliv- ered as the following parts: Part no. Description 210.0010A ROXY Potentiostat, High Current 210.0200D ROXY Potentiostat accessory kit 188.0035... -
Page 15: Installation
7. Follow the installation instructions in the next section of this docu- ment. Fig. 2. ROXY EC with syringe pump. A tutorial movie how to install a ROXY with an µ-PrepCell is available on the Antec website: https://www.antecscientific.com/support/tutorials/roxy-installation... -
Page 16: Electrical Connections
Electrical connections 1. Connect the power cable, trigger cable and serial communication ca- ble to the ROXY Potentiostat as it is shown in the Fig. 3. Use cables provided by Antec or ensure that the used cables meet all relevant safety and EMC and local requirements. - Page 17 Installation guide 17 (of 33) 7. Check in the Windows Device manager the numbers assigned to USB serial ports. Look for “USB Serial Port”. The highest number is “Serial 2” the other is “Serial 1”. Also the Legato port is recognizable as such. In case of doubt unplug the cable and identify the port that disappears in the device manager.
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Page 18: Software Installation
2. After installing Dialogue software, switch on potentiostat and syringe pump. 3. Run the Dialogue software from Windows start menu under 'Antec software'. The program starts with the 'device selector', where active devices and ports can be selected. Choose the correct port numbers to connect Potentiostat and syringe pump, respectively. - Page 19 Installation guide 19 (of 33) 5. The green LEDs in the left bottom corner of the Dialogue window show that the devices are successfully connected. 6. Use the 'Send to device' button to apply changes in settings as the green arrow (right bottom) indicates. 'Read from device' refreshes the current settings.
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Page 20: Installation Of A Flow Cell
2. The schematic drawing how to connect the inlet and outlet tubing with the cell and to place the grounded union is delivered with the ROXY EC system (see Fig. 4). For successful installation it is of utmost importance to install the reactor cell bubble free. - Page 21 Installation guide 21 (of 33) 5. Connect the outlet tubing to the cell and, if the tubing is filled, to MS 6. Ensure that all connections are tight and leak-free. Fig. 4. The schematic of installation of LC connections (180.7169). The details on cell installation, maintenance and troubleshooting are described in the following user manuals: 210.7014 - ReactorCell user manual...
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Page 22: Installation Of Grounding Kit
Of course, the ROXY should be grounded as well, by using a grounded power socket. Fig. 5. The grounding kit inside the ROXY (left) or for external use (right). Performing an experiment The general instruction how to perform an experiment using ROXY EC system are summarized in the steps below. - Page 23 Installation guide 23 (of 33) Do not overtighten the nut holding the WE contact. Screw it gently into the threaded hole until you feel resistance and stop.It is recommended to check if none of the electrodes are short-circuited using a voltmeter. Step 3 - Mobile phase The EC reactor cells require an electrolyte of 10 mM or higher (e.g.
- Page 24 Step 8 – Selecting the operation mode In the ROXY potentiostat the DC and Scan mode are available for efficient metabolite synthesis. The DC mode is based on applying one constant po- tential. Note that the synthesis of different metabolites of one compound may require operation at different potential settings.
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Page 25: Pq Oxidative Mode (Metabolite Synthesis)
PQ Oxidative mode (metabolite synthesis) PQ procedure For a successful performance test it is important that the ROXY EC has been installed as described and that the MS has been optimized for the analysis of the substrate. Set up the system according to the conditions as indicated in table below. -
Page 26: Results
26 (of 33) ROXY installation guide, ed. 4 Results Below mass spectra are from AQ and its oxidative metabolites, measured intensities vary from MS to MS. At 400mV: a dehydrogenation reaction re- sults in m/z 354. At 1200mV several reaction products are found: N- dealkylation (m/z 326), aldehyde formation (m/z 299), oxidation of the alde- hyde to carboxylic acid (m/z 315) and an N-oxidation of AQ (m/z 370). -
Page 27: Pq Reductive Mode (Disulfide Bond Reduction)
PQ Reductive mode (disulfide bond reduction) PQ procedure TiBlue electrode (DC mode) For a successful performance test it is important that the ROXY EC has been installed as described and that the MS has been optimized for the analysis of the substrate. In case a Ti working electrode is used, please follow the instructions in the next chapter. -
Page 28: Pq Procedure Ti Electrode (Pulse Mode)
ROXY installation guide, ed. 4 PQ procedure Ti electrode (Pulse mode) For a successful performance test it is important that the ROXY EC has been installed as described and that the MS has been optimized for the analysis of the substrate. In case a TiBlue working electrode is used, please follow the instructions in the previous chapter. -
Page 29: Results
Installation guide 29 (of 33) Results Below mass spectra are from Insulin and its reductive metabolites, meas- ured intensities vary from MS to MS. The molecule has a light chain and heavy chain that fall apart when the disulfide bridges are reduced. Fig. -
Page 30: Trouble Shooting
When starting up the ROXY it may happen that products are not immediately found in the MS spectra. Give the ROXY and MS some time to stabilize, and wait for the product to reach the MS. - Page 31 Installation guide 31 (of 33)
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Page 32: Dialogue Elite
32 (of 33) ROXY installation guide, ed. 4 C H A P T E R Dialogue Elite Hands-on training This hands-on training is intended for end-users who want to get familiar with the Dialogue software that is used for control and data-acquisition with the ROXY EC system. - Page 33 Check the User manuals & guides for maintenance procedure and op- erational details: ReactorCell user manual p/n 210.7014 µ-PrepCell 2.0 user manual p/n 204.0010A ROXY Potentiostat user manual p/n 210.7010 For tutorials see screen recordings on Antec’s website: https://www.antecscientific.com/support/software-recordings/dialogue-elite- software-tutorials...