Quantabio Q-qPCR Quick Start Manual

Hardware and Software Installation
 Screw in the Bluetooth antenna; or connect the instrument using the USB cable
 Connect the power supply to the instrument and turn the instrument on
A blue light on the front of the instrument illuminates to indicate it is powered on
 Install the Q-qPCR software from provided USB key
The USB key also contains an electronic copy of the user manual
 Open the Q-qPCR software
The instrument will be automatically detected and appear in the top right corner
Reaction Setup
 Load reactions into the pre-racked tubes and use the Capping Tool to help fitting caps properly
 Insert the Tubes into the rotor, ensuring the tab is aligned with the marks on the rotor
To achieve optimum temperature uniformity, it is very important to
insert tubes loaded with water (at reaction volume) into all of the
unused positions on the rotor.
See reverse for more details.
 Secure the tubes into the rotor with the Tube Clamp
Starting a Run
 Select New Run from the toolbar
 Add (Assays+) an assay to the run from the assay list
Additional assays are easily created by selecting New Assay from the toolbar
 Click on the instrument (top right of toolbar) and select Start Run
 Confirm the reaction volume is correct and select Start
A green light on the front of the instrument will indicate the run has begun
 Before analysis, fill in the sample information on the Sample page
 Drag and drop the assay for each well onto the sample editor
Assays are used to link samples together for later analysis
Analysis

Select the required analysis to the run from the options provided
Cycling
Melt
High Resolution Melt (optional)
Absolute Quantification (indicates Standard Curve)
Allelic Discrimination
Relative Quantification

Select the target to analyze and configure the parameters as required
MK-BL-0004 REV 01 Q Quick Start Guide 1018
Quick Start Guide
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